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Electron Microscopy Sciences

Technical Data Sheets

Aldehyde Fuchsin (pH 1.0) Method

EMS Catalog #: 26328-Series    

Fixation:  

10% Buffered Neutral Formalin (#15740) or fixative of choice.

Sections: 

Paraffin at 6µm sections.

Staining Procedures:

  1. Deparaffinize and hydrate to distilled water.
  2. Rinse in several changes of Alcohol, 80% pH 1.0 (#26328-03).
  3. Stain in Aldehyde Fuchsin Solution (#26328-01) for 30 minutes.
  4. Rinse off excess stain with Alcohol, 80% pH 1.0 (#26328-03).
  5. Counterstain with Metanil Yellow Solution (#26328-02) until background is a light yellow (approximately 1 minute). Rinse in distilled water.
  6. Dehydrate in 95% alcohol, absolute alcohol, and clear in Xylene (#23400), two changes each.
  7. Mount with Permount (#17986-01) or DPX (#13510).

Stain Results:

All mucins except the highly acidic sulfated mucosubstances are aldehyde fuchsin negative at pH values of 1.0 and below.

References:

Johnson, W.C., Graham, J.H., and Helwig, E.B., J. Invest. Derm., 42:215-224, 1964.

Luna, L.G., (ed), Manual of Histologic Staining Methods of the AFIP, 3rd edition, McGraw-Hill, c 1968, p.166

Online Ordering

Aldehyde Fuchsin (pH 1.0) Method is available online from the EMS Catalog. For ordering or product information, click here.