French Flag   chineseFlag   japaneseFlag   germanFlag   SpanishFlag   argentinaFlag   mexicoFlag   russianFlag

View Cart || Request a 2017 Catalog

Electron Microscopy Sciences

Technical Data Sheets

Picrosirius Red Staining Of Cardiac Muscle Following Phosphomolybdic Acid Treatment

EMS Catalog #26357

This method eliminates cytoplasmic staining, revealing thin septa and collegen fibers clearly.

Fixation

Bouins' Fixative

Sections

Parrafin @ 7 microns.

Staining

  1. Deparaffinize and hydrate to distilled water.
  2. Wash in running tap water, 10 minutes.
  3. Rinse in distilled water.
  4. Treat in Phosphomolybdic Acid 0.2% Aqueous (1-5 minutes)
  5. Stain in Sirius Red, 0.1% in Sat'd Picric Acid , for 90 minutes.
  6. Wash for 2 minutes in 0.01 N Hydrochloric Acid .
  7. Rinse in 70% Alcohol for 45 seconds.
  8. Dehydrate, clear and mount in Permount

Results

Septa Red

References

Dolber, Paul C. and Madison S. Spach. Picrosirus Red Staining of Cardiac Muscle Following Phosphomolybdic Acid Treatment , Stain Technology , Vol.62 No.1 (1987) 23-26.

Online Ordering

Phosphomolybdic Acid, Sirius Red, and Hydrochloric Acid for Picrosirius Red Staining Of Cardiac Muscle are available online from the EMS Catalog. For ordering or product information, click here.