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Electron Microscopy Sciences

Technical Data Sheets

A Modified Verhoeff Elastic-Van Gieson Stain

EMS Catalog #26369

Consistent and shorter staining time!

Fixation

10% Buffered Neutral Formalin

Sections

4-5 microns

Staining

  1. Deparaffinize and hydrate sections.
  2. Stain in modified Verhoeff at room temperature for 7 minutes or in a 60 °C oven for 3 1/2 minutes.

    To prepare modified Verhoeff Working Elastic solution, mix just before use:
    •  3 parts Alcoholic Hematoxylin, 3%
    •  2 parts Ferric Chloride 2%, aqueous
    •  1 part Lugol's Iodine
  3. Wash in running warm tap water for 1 minute.
  4. Differentiate in Ferric Chloride, 0.4% aq. for 75 seconds.
  5. Wash in running warm tap water for 5 minutes.
  6. Counterstain in Van Geisons solution for 60 seconds.
  7. Dehydrate, clear and mount with Permount.

Results

Nuclei Greyish-black
Elastic Fibers Bluish-black
Collagen fibers Red
Muscle Greenish yellow

References

Garvey, Winsome et al "A modified Verhoff Elastic Van Geison Stain" The Journal of Histotechnology, Vol. 14, No. 2 pp113-114 June 1991.

Online Ordering

Alcoholic Hematoxylin, Ferric Chloride 2% aqueous, Lugol’s Iodine, Ferric Chloride 0.4% aqueous and Van Geisons Solution for a Modified Verhoeff Elastic-Van Gieson Stain are available online from the EMS Catalog. For ordering or product information, click here.