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Electron Microscopy Sciences

Technical Data Sheets

Modified Movats Stain

EMS Catalog #26385

Fixation

10% Buffered Neutral Formalin or Bouin's Solution

Note: If formalin fixative is used, decerated tissue sections must be mordanted in Bouins Fluid for 1 hour in a 50 °C oven. Wash well in running water to remove picric acid deposits.

Sections

4-6 microns

Procedure

  1. Decerate section and hydrate to distilled water.
  2. Stain in Alcian Blue, 1% for 20 minutes.
  3. Dip five times in distilled water.
  4. Place in Alkaline Alcohol in a 56°C oven for 10 minutes.
  5. Wash in running tap water for 2 minutes.
  6. Stain in Orcein-Verhoeff working solution for 2 hours.

    To prepare Orcein-Verhoeff working solution:
    Immediately before use, in the following order, combine the solutions below:

    Orcein 0.2% 25.0 ml
    Alcoholic Hematoxylin 5% 8.0 ml
    Ferric Chloride 10% 5.0 ml
    Lugol's Iodine 5.0 ml

  7. Wash in running tap water for 3 minutes.
  8. Stain in Woodstain Scarlet- Acid Fuchsin working solution for 2 1/2 minutes.
  9. Place in Acetic Acid, 0.5% for 30 seconds.
  10. Differentiate in Phosphotungstic Acid, 5% for 5-10 minutes, well differentiated sections demonstrate colorless collagen and blue-green mucopolysaccharides.
  11. Rinse in Acetic Acid, 0.5% for 30 seconds.
  12. Three changes of 100% ethyl alcohol, 1 minute each,
  13. Stain in Alcoholic Saffron for 8 minutes.
  14. Dehydrate in 100% ethyl alcohol, two changes.
  15. Clear in xylene, three changes.
  16. Coverslip.

Stain Results

Nuclei Black
Cytoplasm Red
Elastic Fibers Purple to Black
Collagen and Bone Yellow
Mucopolysaccharides Blue-Green
Muscle Red

 

References

Movat, H.Z .,: Demonstation of all C onnecti ve Tissue Elements in a Single S ection.
Arch Path., 60:289-295, 1955.

Roman N., et al: Orcein-Hematoxylin in I odize d Ferric Chloride as a Stain for E lastic T issue.
Stain Techn ., 42:199-202, 1967.

Silverman Jefferey., A Modified Movat Pentachrome Stain. Histologic , p. 33. 1972

Online Ordering

Alcian Blue 1%, Alkaline Alcohol, Orcein 0.2%, Hematoxylin Alcoholic 5%, Ferric Chloride 10%, Lugol’s Iodine, Woodstain Scarlet-Acid Fuchsin Working Solution, Acetic Acid 0.5%, Phosphotungstic Acid 5%, and Alcoholic Saffron for Modified Movat's Stain are available online from the EMS Catalog. For ordering or product information, click here.