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Electron Microscopy Sciences

Technical Data Sheets

Toren's Method For Mast Cells

EMS Catalog #26472

Fixation

10% Buffered Neutral Formalin

Section

Paraffin, @ 6 Microns

Staining Procedure

  1. Deparaffinize and hydrate to distilled water.
  2. Stain in *Working Giemsa Solution for 75 minutes. Rinse in distilled water.

    ***Prepare Working Giemsa Solution by adding 40 drops of
    Giemsa Stock Solution, to 40ml of distilled water and mixing thoroughly.
    Prepare fresh, immediately before use.
  3. Place in Acetic Acid, 0.5%, for 5 to 10 seconds. Rinse in distilled water.
  4. Stain in Pollack's Trichrome Solution, for 10 to 15 seconds.
  5. Rinse in Acetic Acid, 0.5%, until no more stain washes off, 3 to 5 seconds.
  6. Dehydrate in 95% Alcohol, Absolute Alcohol and clear in Xylene.
  7. Mount with Permount.

Results

Mast Cell Granules deep blue
Collagen & Cartilage blue green
Muscle & Elastic Fibers red
Fibrin purple
Bone green
Colloid, Keratin & Erythrocytes orange red to orange

References

Toren, D.A.: Stain Techn ., 38:249-250, 1963.

Luna, L., (ed.), Manual of Histologic Staining Methods of the AFIP , 3 rd . ed.,
McGraw-Hill , New York , c. 1968, pp. 116-117.

Online Ordering

Giemsa Stain Solution, Acetic Acid 0.5% Aqueous and Pollacks’s Trichrome Solution for Toren's Method For Mast Cells are available online from the EMS Catalog. For ordering or product information, click here.