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Electron Microscopy Sciences

Technical Data Sheets

Lillies Method For Ferric and Ferrous Iron

EMS Catalog Number: 26604-Series

Fixation:

10% Buffered Neutral Formalin (#15740).

Sections:

Paraffin @ 6 microns

Staining Procedures:

(Use control slide. Use chemically clean glassware)

Preparation of Potassium Ferrocyanide Solution:

Prepare fresh, just before use

  • Potassium Ferrocyanide (#26604-01) - 0.4 g
  • Hydrochloric Acid (#26604-02) - 40.0 ml

Preparation of Potassium Ferricyanide Solution:

Prepare fresh just before use.

  • Potassium Ferricyanide (#26604-1A) - 0.4 g
  • Hydrochloric Acid (#26604-02) - 40.0 mg
  1. Deparaffinize and hydrate to distilled water.
  2. For Ferric Iron, place in Potassium Ferrocyanide Solution for 1 hour. For Ferrous Iron, place in Potassium Ferricyanide Solution for I hour.
  3. Wash in Acetic Acid, 1% (#26604-04).
  4. Stain for 10 minutes in Basic Fuchsin Solution (#26604-03). Rinse in distilled water, (For critical work, omit this step).
  5. Dehydrate in 95% alcohol, absolute alcohol and clear in xylene, two changes each.
  6. Mount with Permount (#17986-01)

Stain Results:

Ferric Iron Dark Prussian’ Blue
Ferrous Iron Dark Turnbull’s Blue
Background Light Red

References:

Lillie, R.D., Histopathologic Technic and Practical Histochemistry, 3rd ed. McGraw-Hill, N.Y., p.407, c 1965.

Luna, L.G. (ed). Manual of Histologic Staining Methods of the Armed Forces Institute of Pathology, 3rd ed., McGraw-Hill, N.Y., c. 1968, p.182.

Online Ordering

Lillies Method For Ferric and Ferrous Iron is available online from the EMS Catalog. For ordering or product information, click here.