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Electron Microscopy Sciences

Technical Data Sheets

Oil Red O Method For Lipofuchsin

EMS Catalog #26609


10% Buffered Neutral Formalin


Paraffin at 6 microns

Staining Procedure

  1. Deparaffinize and hydrate to distilled water.
  2. Stain in Oil Red O Solution for 72 hours.
  3. Agitate the slide in Propylene Glycol, 85% for 1 minute.
  4. Two changes of distilled water, 2 minutes each
  5. Stain in Mayer's Hematoxylin for 5 minutes. Wash in running water for 10 minutes.
  6. Mount with Glycerin Jelly

Staining Results

Lipofuchsin Red
Nuclei Blue


Luna, L.G. (ed.), Manual of Histologic Staining Methods of the AFIP , 3 rd edition, The AFIP, 3 rd edition, McGraw-Hill, NY, P. 186, c 1968.

Online Ordering

Oil Red O Solution 0.5%, Propylene Glycol 85% Aqueous and Mayer’s Hematoxylin for Oil Red O Method for Lipofuchsin are available online from the EMS Catalog. For ordering or product information, click here.