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Electron Microscopy Sciences

Technical Data Sheets

Prussian Blue Method for Hemosiderin

EMS Catalog #: 26613-Series

Fixation:

10% Buffered Neutral Formalin (#15740).

Sections:

Paraffin @ 5 microns

Staining Procedures:   

  1. Deparaffinize and hydrate to distilled water.
  2. Place sections in working staining solution* for 30 minutes @ 60°C or for 1 hour at room temperature. Rinse in distilled water.
    1. Prepare working stain by mixing equal volumes of Potassium Ferrocyanide, 2% Aqueous (#26613-01) and Hydrochoric Acid, 2% (#26613-02). Prepare fresh just before use.
  3. Counter-stain in Safranin O, 0.2% (#26613-03) for 2 minutes. Wash in Acetic Acid, 1% (#26613-04)
  4. Dehydrate in 95% Alcohol, absolute alcohol, and clear in xylene, two changes each.
  5. Mount with Permount (#17986-01) or any synthetic resin.

Stain Results:  

Hemosiderin Blue or Green
Nuclei Red
Background Pink

References:

Perls, M., Virchov’s Arch. F. Path. Anat., 39:42-48, 1867.

Highman, B., Arch. Path., 33:937-938, 1942.

Lillie, R.D. Histopathologic Technic and Practical Histo-chemistry, 3rd edition, McGraw-Hill, N.Y., 1965.

Clark, G. (ed). Staining Procedures, 3rd ed., Williams & Wilkins, Baltimore, p. 157 c. 1973.

Online Ordering

Prussian Blue Method for Hemosiderin is available online from the EMS Catalog. For ordering or product information, click here.