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Electron Microscopy Sciences

Technical Data Sheets

Vogt's Method for Nerve Cell Products

EMS Catalog #26671-Series

Fixation

Formalin, 10% Neutral Buffered (#15740).

Sections

Paraffin @ 6 microns

Staining

  1. Deparaffinize and carry through absolute alcohol, two changes.
  2. Let stand in absolute alcohol for two hours.
  3. Stain in Cresyl Violet Acetate Working Solution (#26671-1A) for 40 – 60 minutes.
  4. Differentiate rapidly in 95% alcohol, then dehydrate in absolute alcohol, and clear in xylene, two changes each.
  5. Mount with Permount (#17986-01)

Staining Results

Nissl Substances

Intense Purple

Nuclei

Purple

Background

Clear

References

AFIP Manual of Histologic Staining Technique; ed. L. Luna., 3rd ed. McGraw-Hill Publications, c. 1968, p. 212.

Ordering

Vogt’s Method for Nerve Cell Products