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Electron Microscopy Sciences

Technical Data Sheets

Darrow Red

EMS Catalog #26674

Fixation

Will follow most fixatives; Formalin is usually used.

Sections

Paraffin, Frozen or Celloidin

Staining

  1. Stain well washed Weil stained sections or Kluver-Barrera stained sections (with either of these the sections should be no thicker than 20 µ) or unstained sections for 20 minutes in Darrow Red Staining Solution.
  2. Pass rapidly through ascending alcohols.
  3. Two changes of n-butyl alcohol for a total of 4 minutes.
  4. Terpineol: Xylene (1:4) overnight.
  5. Clear and mount.

Staining Results

Nissl Bodies and Nuclear Chromatin Red

References

Clark, G.: Staining Procedures , Williams and Wilkins Co., Baltimore , 3 rd Ed., c. 1973, p. 89.

M.M., Clark, G., Darrow, M.A. and Emmel, V.M., Staining Techn ., 35:19-21, 1960.

Powers, M.M. and Clark, G., Stain Techn ., 38:289, 1963.

Ordering

Darrow Red Staining Solution, N-Butyl Alcohol and Terpineol-Xylene 1:4 for Darrow Red are available by calling EMS.