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Electron Microscopy Sciences

Technical Data Sheets

Woelck’s Method for Myelin Sheath

EMS Catalog #: 26684-Series

Fixation:

Formalin 10% Neutral Buffered (#15740).

Sections:

Paraffin @ 15 microns.

Staining Procedures:

  1. Deparaffinize and hydrate to distilled water.
  2. Mordant in Ferric Ammonium Sulfate, 2.5% (#26684-01) overnight. Rinse in two changes of distilled water.
  3. Working Hematoxylin solution for 2 hours. To prepare working solution mix the following ingredients in order listed just before use:
    45.0 ml Distilled water
    10.0 ml Hematoxylin Stock (#26684-02) – filtered
    7.0 ml Lithinum Carbonate, Saturated (#26684-03) – filtered
    45.0 ml Distilled water
  4. Rinse in two changes of distilled water.
  5. Differentiate in 80% Alcohol until background is clear and myelin stands out sharply.
  6. Dehydrate with 95% Alcohol, absolute alcohol, and clear in Xylene (#23400) two changes each. Mount.

Stain Results:  

Glial Cells, Nucleoli of Neurons Black
Myelin Sheath Blue
Background Clear

References:

AFIP, Manual of Histological Staining Methods, 3rd ed., Ed. L. Luna: New York: McGraw-Hill Publications. C. 1968, p. 206.

Online Ordering

Woelck’s Method for Myelin Sheath is available online from the EMS Catalog. For ordering or product information, click here.