French Flag   chineseFlag   japaneseFlag   germanFlag   SpanishFlag   argentinaFlag   mexicoFlag   russianFlag

View Cart || Request a 2019 Catalog

Electron Microscopy Sciences

Technical Data Sheets

EMS Rapid Pro Kit Protocols

EMS Catalog #: 26750-Series

Protocols for Automatic Processing Machines

General approach is 1 min of each station from 1 to 7 per 1x1mm of tissue thickness(size), so if tissue is 1x1mm it should be kept in every station for 1 minutes(see below)

In case you want to perform even faster microscopic analysis of the specimen you can increase the temperature of all Stations (steps) from 1 to 7.

Temperature of all Stations (1-7) with processing solutions should be kept at 46-50° Celsius.

Temperature of paraffin stations should be kept at 60° Celsius.

In case of troubleshooting, please refer to routine formalin processing adjusting to processor time

Protocols

  1. Speedy with vivid picture: protocol #1 Time: 20 min Tissue Size: 0.5x0.5mm to 2x2mm
  2. Speedy with vivid picture: protocol #2 Time: 30 min Tissue Size: 1x1mm to 4x4mm
  3. Speedy with vivid picture: protocol #3 Time: 60 min Tissue Size: 1x1mm to 4x4mm including the vessel 5x2 mm
  4. Fast: protocol #4 Time: 39 min Tissue Size: 0.5x0.5mm to 4x4mm
  5. Fast: protocol #5 Time: 46 min Tissue Size: 2x2mm to 4x4mm
  6. Fast: protocol #6 Time: 53 min Tissue Size: 4x4mm to 5x5mm
  7. Fast: protocol #7 Time: 1 hr 75 min Tissue Size: 5x5mm to 10x10mm
  8. Classic: protocol #8 Time: 46 min Tissue Size: 1x1mm to 2x2mm
  9. For larger tissue samples: protocol #9 Time: 60-150 min Tissue Size: 1cm to 2cm
P# Station Cutting water bath temp
  1
PPS1
2
PPS2
3
PPS3
4
PPS4
5
PPS4
6
PPS5
7
PPS5
8-10
Skip
11
Paraffin
12
Paraffin
13
Paraffin
14 Skip
(Paraffin)
1 2 min. 2 min. 2 min. 2 min. 2 min. 2 min. 2 min. Skip 2 min. 2 min. 2 min. Skip 36-40°C
2 3 min. 3 min. 3 min. 3 min. 3 min. 3 min. 3 min. Skip 3 min. 3 min. 3 min. Skip 36-40°C
3 6 min. 6 min. 6 min. 6 min. 6 min. 6 min. 6 min. Skip 6 min. 6 min. 6 min. Skip 36-40°C
4 2 min. 2 min. 2 min. 2 min. 2 min. 2 min. 2 min. Skip 5 min. 10 min. 10 min. Skip 30-36°C
5 3 min. 3 min. 3 min. 3 min. 3 min. 3 min. 3 min. Skip 5 min. 10 min. 10 min. Skip 30-36°C
6 4 min. 4 min. 4 min. 4 min. 4 min. 4 min. 4 min. Skip 5 min. 10 min. 10 min. Skip 36-40°C
7 5-10 min. 5-10 min. 5-10 min. 5-10 min. 5-10 min. 5-10 min. 5-10 min. Skip 5 min. 10 min. 10 min. Skip 36-40°C
8 3 min. 3 min. 3 min. 3 min. 3 min. 3 min. 3 min. Skip 5 min. 5 min. 5 min. Skip 46-47°C
9 10-15 min. 10-15 min. 10-15 min. 10-15 min. 10-15 min. 10-15 min. 10-15 min. Skip 10-15 min. 10-15 min. 10-15 min. Skip 25-30°C

Product Information

EMS Rapid Pro Processing System