EMS Rapid Pro Kit Protocols
EMS Catalog #: 26750-Series
Protocols for Automatic Processing Machines
General approach is 1 min of each station from 1 to 7 per 1x1mm of tissue thickness(size), so if tissue is 1x1mm it should be kept in every station for 1 minutes(see below)
In case you want to perform even faster microscopic analysis of the specimen you can increase the temperature of all Stations (steps) from 1 to 7.
Temperature of all Stations (1-7) with processing solutions should be kept at 46-50° Celsius.
Temperature of paraffin stations should be kept at 60° Celsius.
In case of troubleshooting, please refer to routine formalin processing adjusting to processor time
Protocols
- Speedy with vivid picture: protocol #1 Time: 20 min Tissue Size: 0.5x0.5mm to 2x2mm
- Speedy with vivid picture: protocol #2 Time: 30 min Tissue Size: 1x1mm to 4x4mm
- Speedy with vivid picture: protocol #3 Time: 60 min Tissue Size: 1x1mm to 4x4mm including the vessel 5x2 mm
- Fast: protocol #4 Time: 39 min Tissue Size: 0.5x0.5mm to 4x4mm
- Fast: protocol #5 Time: 46 min Tissue Size: 2x2mm to 4x4mm
- Fast: protocol #6 Time: 53 min Tissue Size: 4x4mm to 5x5mm
- Fast: protocol #7 Time: 1 hr 75 min Tissue Size: 5x5mm to 10x10mm
- Classic: protocol #8 Time: 46 min Tissue Size: 1x1mm to 2x2mm
- For larger tissue samples: protocol #9 Time: 60-150 min Tissue Size: 1cm to 2cm
| P# | Station | Cutting water bath temp | |||||||||||
| 1 PPS1 |
2 PPS2 |
3 PPS3 |
4 PPS4 |
5 PPS4 |
6 PPS5 |
7 PPS5 |
8-10 Skip |
11 Paraffin |
12 Paraffin |
13 Paraffin |
14 Skip (Paraffin) |
||
| 1 | 2 min. | 2 min. | 2 min. | 2 min. | 2 min. | 2 min. | 2 min. | Skip | 2 min. | 2 min. | 2 min. | Skip | 36-40°C |
| 2 | 3 min. | 3 min. | 3 min. | 3 min. | 3 min. | 3 min. | 3 min. | Skip | 3 min. | 3 min. | 3 min. | Skip | 36-40°C |
| 3 | 6 min. | 6 min. | 6 min. | 6 min. | 6 min. | 6 min. | 6 min. | Skip | 6 min. | 6 min. | 6 min. | Skip | 36-40°C |
| 4 | 2 min. | 2 min. | 2 min. | 2 min. | 2 min. | 2 min. | 2 min. | Skip | 5 min. | 10 min. | 10 min. | Skip | 30-36°C |
| 5 | 3 min. | 3 min. | 3 min. | 3 min. | 3 min. | 3 min. | 3 min. | Skip | 5 min. | 10 min. | 10 min. | Skip | 30-36°C |
| 6 | 4 min. | 4 min. | 4 min. | 4 min. | 4 min. | 4 min. | 4 min. | Skip | 5 min. | 10 min. | 10 min. | Skip | 36-40°C |
| 7 | 5-10 min. | 5-10 min. | 5-10 min. | 5-10 min. | 5-10 min. | 5-10 min. | 5-10 min. | Skip | 5 min. | 10 min. | 10 min. | Skip | 36-40°C |
| 8 | 3 min. | 3 min. | 3 min. | 3 min. | 3 min. | 3 min. | 3 min. | Skip | 5 min. | 5 min. | 5 min. | Skip | 46-47°C |
| 9 | 10-15 min. | 10-15 min. | 10-15 min. | 10-15 min. | 10-15 min. | 10-15 min. | 10-15 min. | Skip | 10-15 min. | 10-15 min. | 10-15 min. | Skip | 25-30°C |
