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Electron Microscopy Sciences

Technical Data Sheets

Harris Hematoxylin (With or Without Mercury)

EMS Catalog #: 26754-Series

Fixation:

Formalin 10% Neutral Buffered (#15740) or fixative of choice

Sections:

Paraffin @ 5 -6 microns

Staining Procedures:

  1. Deparaffinize and hydrate to distilled water
    1. Stain in Harris Hematoxylin (#26754-01) containing 2 – 4 ml Glacial Acetic Acid per 100 ml for 5 – 15 minutes. Wash in water.
    2. If using Harris Hematoxylin w/o Mercury (#26754-1A) containing 2 – 4 ml Glacial Acetic Acid per 100 ml for 5 – 15 minutes. Wash in water.
  2. Differentiate in Acid Alcohol (#26754-03) until the sections turn red.
  3. Blue section in Sodium Acetate, 1% (#26754-04) or Ammonium Water (#26754-4A) or Lithium Carbonate Solution 26754-4B).
  4. Wash thoroughly and counterstain.

References:

Harris, H.F., J. Appl. Microscopy, III: 777-781, 1900.

Mallory, F.B., Pathological Technique. W.B. Saunders Co, Philadelphia, 1938.

Clark, G., (ed). Staining Procedures, 3rd edition, Williams & Wilkins, Baltimore, pp 33-35, 1973.

Luna, L.G. (Ed), Manual of Histologic Staining Methods of the AFIP, 3rd Edition, McGraw-Hill, NY, pp 34 and 38, c. 1968.

Online Ordering

Harris Hematoxylin (With or Without Mercury) is available online from the EMS Catalog. For ordering or product information, click here.