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Electron Microscopy Sciences

Technical Data Sheets

Periodic Acid Leucofushin Method

EMS Catalog #: 26853- Series

Fixation:

Zenker Fluid or 10% Formalin

Sections:

Paraffin @ 6 microns

Staining Procedures:            

  1. Deparaffinize and hydrate to distilled water.
  2. Rinse in tap water. Oxidize in Periodic Acid 1% Aqueous for ten minutes. Wash five minutes in running water.
  3. Stain ten minutes with Schiff’s Reagent
  4. Pass directly to three successive baths of two minutes each in Sodium Bisulfite 0.05M. Wash in running tap water for ten minutes.
  5. Stain in Weigert’s Hematoxylin or Mayer’s Acid Hemalum for two to five minutes, wash in tap water.
    To prepare Weigert’s Hematoxylin:
    Mix equal parts of Weigert’s A and Weigert’s B just before use.
    Counterstain for one minute woth Picric Acid, Saturated (Aq) or Orange G, 1%. (A gram-Weigert staining procedure may be substituted for steps 6-8).
  6. Dehydrate in two changes each of 95% and absolute alcohol. Clear through Alcohol-Xylene and two changes of Xylene
  7. Mount in Clarita.

Stain Results:  

Nuclei
Black on Blue
Collagen
Pink (Orange if Picric Acid is used as counterstain)
Reticulm Purplish Red (Orange-red if Picric Acid is used)
Glycogen- Dark Purplish Red
Epithelial Mucin Red-Purple to Violet
Filbrin Pink (Pink to Violet if the Weigert Fibrin Variant was used as a counterstain)
Cytoplasm Gray, yellow or Orange (depending on the counterstain)

References:

Clark, G: Staining Procedures, Williams & Wilkins. Co., Baltimore, 3rd Ed. C.1973, P. 156.

Online Ordering

Periodic Acid Leucofushin Method is available online from the EMS Catalog. For ordering or product information, click here.