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Electron Microscopy Sciences

Technical Data Sheets

Neat Stain Hematology Stain Kit

EMS #27104-01

Three-step procedure for differentiation of morphological cell types in peripheral blood smears. Also stains microorganisms and spermatozoa. Neat Stain is for manual staining of blood smears. The staining characteristics are similar to the traditional Wright's and Wright-Giemsa stains. Neat Stain offers the flexibility of a three-to -five dip staining sequence in both the stain and counter-stain solution.

Storage Conditions

Store at controlled room temperature (15-30°C). Keep cuvettes tightly sealed when not in use. Do not use after specified expiration date.

Indications of Deterioration

Mold growth or excessive precipitation in reagents are indications of contamination and or degradation.

Specimen Collection & Preparation

Smears prepared from fresh uncoagulated venous or arterial blood should be air-dried. If the smears are not prepared immediately, 2mgs of EDTA/ml of blood sample should be added to prevent coagulation.

Procedure

Materials provided: Neat Stain Hematology Cuvettes (6), Stand & Foil Closures
Materials Not Provided: Microscope Slides; immersion oil

Technique

  1. Carefully open foil sealed cuvette. When not in use, reseal with supplied foil sheet(s).
  2. Prepare slides as instructed under Specimen Collection.
  3. Dip each slide to be processed for five, one second dips into the Fixative Solution. Allow excess reagent to drain or blot slide.
  4. Repeat this procedure substituting Solution I and Solution II respectively.
  5. Rinse slides with deionized water and allow to dry. Examine using immersion oil.

Note:

Staining intensity may be varied by increasing or decreasing the number of dips in Solution I or Solution II. For a more eosinophilic result (red intensity), increase the number of dips into Solution I; for a more basophilic result (blue intensity), increase the number of dips into Solution II. Ideally, five one second dips into each Solution will produce the desired results. Any variation of the procedure other than described above may adversely affect the results.

Stained Specimen Results

Specimen Nucleus Granules Cytoplasum
Erythrocytes n/a n/a Pink
Leukocytes Granular      
Polymorphonuclear      
Neutrophils Purple Red-lilac Light pink
Eosinophils Dark blue Red/orange Medium blue
Basophils Dark blue Dark purple Light blue
Leucocytes Nongranular Monocytes Violet n/a Light blue
Lymphocytes Violet n/a Medium blue
Platelets n/a Purple Lilac
Microorganisms Blue/violet n/a n/a
Spermatozoa Dark blue    

Precautions

Neat Stain, Fixative Solution contains methyl alcohol and is a Flammable Liquid.

Danger

– Poison may be fatal or cause blindness if swallowed. Vapor is harmful and will cause eye irritation. Do not breathe vapor. Avoid contact with eyes, skin or clothing. Keep container closed. Use with adequate ventilation. Keep from heat, sparks and open flame. Wash thoroughly after handling.

Cannot be made nonpoisonous.

First Aid:

If swallowed, call a physician immediately. In case of contact with eyes, immediately flush with water for 15 minutes and notify a physician. Wash exposed areas thoroughly after handling.

Warning:

Solution I contains trace amounts of sodium azide which may react with copper and lead plumbing to form highly explosive metal azides. On disposal, flush with a large volume of water to prevent azide build-up.

Online Ordering

Neat Stain Hematology Stain Kit is available online from the EMS Catalog. For ordering or product information, click here.