A flexible thermoplastic fluoropolymer films with high optical clarity and a smooth surface.
Macrophages selectively adhere to its surface. Lymphocytes do not. ACLAR® films can be cut into discs (using our Disc Punches, Cat #77850-08 for disc size 7.94mm diameter; Cat. #77850-09 for disc size 9.54mm diameter; Cat. #77850-12 for disc size 12.7mm diameter, and Cat #77850-25 for disc size 25.4mm diameter), as a substrate in standard tissue culture, after washing, can produce an almost pure collection of macrophages. Discs can then be fixed, dehydrated, critical point dried and attached to SEM specimen mount stubs.
For HRP studies, ACLAR® film is helpful for LM evaluation, prior to thin sectioning. After processing through HRP , fixation and dehydration series and embedding procedure schedules, one can place a slice of tissue sample with a drop of epoxy resin on a small piece of ACLAR®. Film. Place another piece of ACLAR® on top, and press between two pieces of glass slide. Polymerize in the oven. Once polymerized, ACLAR® film peels off easily to reveal a thin plate of embedded tissue which is conveniently examined under the light microscope
"Ultrastructure of HRP -Labelled Neurons: A Comparison of Two Sensitive Techniques." G.M Mawe, J.C. Bresnahan and M.S. Beattie. Brain Research Bulletin, Vol. 10, pp. 551 - 558, 1983.
"Fluoroplastic Coverslip for Long-Term Nerve Tissue Culture. " Edmund B. Masurovsky and Richard P. Bunge. Stain Technology, Vol. 43, No 3, 1968.
Kingsley R. E., Cole N. L. "Preparation of cultured mammalian cells for transmission and scanning electron microscopy using ACLAR FILM ". J. Of Electron Microscopy Technique, 10, pp. 77-85, 1988.
Larue D. T., Wimer J. A. "Postembedding immunocytochemistry of large sections of brain tissue: an improved flat-embedding technique". J. Of Neurosciences Methods", 68, pp125-132, 1996
Stratton C. J., Baguinov Y, Sanders K. M., Ward S. M. "Ultrastructural analysis of the transdifferentiation of smooth muscle to skeletal muscle in the murine esophagus. Cell Tissue Research, 301, p 284, 2000