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Electron Microscopy Sciences

Technical Data Sheets

Leica Bright-Line Counting Chamber

EMS Catalog #63514-10, 63514-11, 63514-12 and 63514-14

Directions for Use:

Usage Cell counts.
Cell Depth 0.100mm +/- 2% (1/10mm)
Volume 0.1 Microliter
Ruling Pattern Improved Neubauer, 1/400 Square mm

Rulings cover 9 square millimeters. Boundary lines of the Neubauer ruling are the center lines of the groups of three. (These are indicated in the illustration below.) The central square millimeter is ruled into 25 groups of 16 small squares, each group separated by triple lines, the middle one of which is the boundary. The ruled surface is 0.10mm below the cover glass, so that the volume over each of the 16 small squares is .00025 cubic mm.

Neubauer Ruling

Neubauer Ruling

The number of cells per cubic millimeter = 
         Number of cells counted per square millimeter X dilution (if used) X 10

The number of cells per milliliter = 
        Number of cells counted per square millimeter X dilution (if used) X 10,000

One (1) Milliliter = 1000 cubic millimeters (cu mm)

One (1) Microliter (ul) = One (1) cubic millimeter (cu mm)


After completing the count, remove the cover glass and clean the counting chamber with water or a mild cleaning solution (10% solution of bleach). Dry the counting chamber with a soft cloth or wipe, or rinse with acetone.

Online Ordering

Bright-Line Chamber is available online from the EMS Catalog. For ordering or product information, click here.