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Electron Microscopy Sciences

Technical Data Sheets

PEPSIN Reagent, Ready to use, Antigen Retriever

EMS Catalog #64142-05, 64142-06

Description

Pepsin is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. Pepsin digestion of FFPE tissue section improves accessibility of antibodies to tissue antigens.

Storage

2-8°C, DO NOT FREEZE

Intended Use

Use as antigen retriever for some antibodies in IHC. Please refer to primary antibody protocol for IHC.

Reagent

Ready-to use Pepsin reagent.

Procedures

  1. Deparafinize FFPE tissue section is carried out as outlined in your protocol. Hydrate tissue with PBS or ImmunoBioScience Universal Immuno buffer.
  2. Remove buffer, add 2-4 drops of Pepsin reagent to cover tissue section.
  3. Incubate at 37°C or at 56°C for 5-10 minutes (the time required for optimum digestion of FFPE tissue section will vary with the extent of fixation, generally approx. 5-10 minutes are sufficient at the above temperatures. In some cases it may require 15 minutes).
  4. Discard Pepsin reagent, wash 3-5 times with buffer.
  5. The tissue sections are ready for further IHC protocol.

NOT RECOMMENDED FOR CYTOKERATIN (clone: AE3) ANTIBODY

Limitation: Over-digestion may detach tissue sections from slides or may result in the loss of tissue morphology. It may be necessary to use + charge, sialanized or polylysine coated slides.

Limitation and Warranty

Our warranty is limited to the actual price paid for the product. We are not liable for any property damage, personnel injury, time, effort or economic loss due to our product.

MSDS

This product contains 0.05% Proclin 300 as a preservative. Avoid skin and eye contact with all laboratory products. Use appropriate laboratory gear, laboratory coat, gloves and safety glasses. Do not ingest any laboratory products. This product is not approved for administration in human or animals.

For research use only; not for use in diagnostic procedures. FOR IN VITRO LABORATORY USE ONLY

Country of origin: USA

Online Ordering

Antigen Retrievers are available online from the EMS Catalog. For ordering or product information, click here.