Biobond Tissue Section Adhesive
For immunohistochemistry the choice of specimen preparation is critical for the preservation of antigens in the sample. Of greatest importance in the preparation schedules are the specimen fixation and embedding. The protocol must satisfy the requirements for preservation of structural integrity and antigenicity.
Why Coat Slides?
Having prepared the tissue specimen for immunolabelling it is then imperative to perform the incubations with a protocol designed to maximize the specific signal and minimize the background. Some incubation conditions may cause tissue sections to be removed from the glass slide. Typical tissue section adhesives such as poly-L-Lysine, Elmer’s glue, chrome alum, et cetera, are not suitable for use with Immunogold labeling because of the increased background caused by attraction of gold particles to the adhesive on the slide.
In addition the surface of glass slides is uneven and is activated by the silicon tetrahedral structure. It therefore provides active sites for absorption of proteins or reactions with chemicals and reagents. It is therefore important to minimize this possibility by coating the surface with a material that is of low reactivity towards reagents.
Biobond produces a very strong adhesion between the glass and the tissue section for subsequent incubations. Biobond coats the glass slide with a protective layer to minimize interaction of charged glass surface with reagents. This is also of particular importance for reproducibility of results because of the variations that occur between glass slides obtained from different sources and in different countries. It is particularly effective for use with sever incubating conditions such as those used in situ hybridization.
Simple Coating Procedure
With Biobond there are not complicated procedures for coating slides. The following protocol will coat 100 or more slides.
Wear disposable rubber gloves while handling Biobond. Please contact our technical services department for extra copies of full safety instructions enclosed with this product.
- Load racks (metal or plastic) with slides. A rack of 25 at a time is most convenient.
- Place the racks in a 2% solution of Decon detergent and leave soaking for 1 hour to clean.
- Rinse thoroughly with tap water to remove all traces of Decon. If the local tap water is unclean or very hard then use distilled water.
- Air-dry the slides at room temperature or at 40°C. Cover to protect from dust.
- Prepare a 2% solution of Biobond in acetone (2ml in 100ml). General-purpose reagent will be sufficient.
- Dip the rack of slides in Biobond solution for 4 minutes.
- Rinse for 5 minutes in clean tap water or distilled water.
- Air dry
- Once the slides are completely dry store in a dust free environment until needed (usually in the original slide box).
The slides are then ready for mounting sections. They may be stored indefinitely, A 100ml solution of 2^ Biobond will coat at least 100 slides by this method.
Biobond is suitable for all kinds of tissue specimens including paraffin wax or resin sections, cell smears, cytospins or cryostat sections. Biobond is supplied in 20ml unit volumes, sufficient to coat at least 100 slides.
For further assistance with any of our reagents please feel welcome to call our Technical Services Department.