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Electron Microscopy Sciences


arrow13Glow Discharge System for TEM Grids

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EMS GloQube™
  • EMS GloQube™ Start-up Screen
  • EMS GloQube™ Clean Chamber
  • EMS GloQube™ Vapor Chamber
  • EMS GloQube™ Vapor Delivery System
  • EMS GloQube™ Vapor Delivery System
  • EMS GloQube™ Easy Sample Loading
  • EMS GloQube™ Easy Sample Loading
  • EMS GloQube™ Easy Sample Loading
  • EMS GloQube™ Stored Profiles
  • EMS GloQube™ Selecting a New Profile
  • EMS GloQube™ A Typical Process Run
  • EMS GloQube™ Clean Chamber During a Process Run
  • EMS GloQube™ Pumping cycle
  • EMS GloQube-D and Optional Pfeiffer DUO 6 Rotary Pump

arrow13EMS GloQube™

Rapid, reliable results with the EMS GloQube™

The EMS GloQube™ is the first-of-its-kind compact, easy to use, stand-alone glow discharge system.

Unique Dual Chamber Processing, Safe Handling of Reagents

The GloQube has two independent vacuum chambers: a clean chamber, designed for applications requiring hydrophobic/hydrophilic conversion, typically using air as the process gas; and a vapor chamber, designed for use with reagents such as methanol and alkylamine. With operator safety firmly in mind, reusable septum-sealed reagent vials are used. Loading and removing reagents is convenient and reliable – the vial, located in its holder, is inserted into a shielded needle using a simple bayonet fitting.

To prevent accidental damage, the high voltage lead is shielded. The plasma current is variable by adjustment of the vacuum level using an argon leak valve with the plasma voltage being preset. For maximum sputter coating efficiency, the gas injector system ensures that argon gas enters the chamber close to the plasma discharge. Venting is to argon.

Key Features

  • Dual independent chambers
  • Hydrophilic/hydrophobic and negative/positive modes
  • Fully automatic, short process times
  • Intuitive touch screen control
  • Safe vapor delivery using septum-sealed vials
  • Automatic valving between chambers to prevent cross-contamination
  • Quick and easy sample loading
  • Controlled venting to prevent sample disturbance
  • Consistent, reliable results
  • Three-year warranty

The primary application of the EMS GloQube™ is the hydrophilization (wetting) of carbon-coated TEM support films and grids which otherwise have the tendency to be hydrophobic. Glow discharge treatment with air will make film surfaces negatively charged and hydrophilic and allow the easy spread of aqueous solutions. This and other processes are outlined below.

Glow Discharge Process
Surface State Charge Atmosphere Typical applications
Hydrophilic Negative Air Carbon coated TEM grids
Hydrophilic Positive Air – with magnesium acetate post-treatment Nucleic acid adhesion to carbon films
Hydrophilic Positive Alkylamine Proteins, antibodies and nucleic acids
Hydrophilic Negative Methanol Positively charged protein molecules
(e.g. ferritin, cytochrome c)

Easy sample loading, fast turnaround times

Each chamber can accommodate two 25 x 75 mm glass microscopes slides. Loading could not be easier using draw-style chamber doors and specimen stages. The stages are height adjustable and fitted with removable glass slide holders. For additional convenience – and to allow easy access for chamber cleaning – the stages can be completely removed.

Touch screen control – rapid data input, simple operation

The intuitive touch screen allows multiple users to rapidly input and store preferred process "recipes". Typical default glow discharge protocols are loaded as standard. Additionally, help files and useful maintenance data such as system on time and time since last clean are readily available to the operator. An Ethernet communications port is included for software updates.

Vacuum, automatic valving and controlled venting

The GloQube™ has automatic valving between chambers which maintains cleanliness by preventing cross-contamination. At the end of a process run, automatic soft venting to atmosphere through filtered inlets ensures TEM grids are not disturbed. The GloQube™ requires a single vacuum pump working in the 0.1 to 1 mbar range. A typical pump time to operational vacuum is 60 seconds.


Power and Processes
Plasma current 1-40 mA
HV power supply 30 W
Maximum voltage 800 V
Electrode polarity – clean chamber DC glow positive DC glow negative
Electrode polarity – vapor chamber DC glow positive DC glow negative
Sample stage 125 x 100 mm (4.9" x 3.94") with location for two 25 x 75 mm (1" x 3") glass slides
Sample stage operational heights Adjustable 12.5 mm (0.5"), 22.5 mm (0.9") or 35 mm (1.38")
Pump hold time requirement 0-24 hours
Process time 1-600 seconds
Chamber vent inlets Filtered air inlets with slow vent to minimise sample disturbance
On-board reagent storage Reagents (e.g. methanol or alkylamine) are contained in reusable sealed glass vials to minimize exposure to hazards. (GloQube-D only)
High voltage safety interlocks Hardware safety interlocked and software for process control
Vacuum control Intergrated pirani gauge
Working vacuum range 0.1 to 1 mbar
Vacuum pump minimum requirements 6 m3/hr, 3600 l/m, 0.03 mbar ultimate vacuum. Inlet flange: KF 16
Pumping time Typical pump time to an operational vacuum of 0.27 mbar in 60 seconds
Vacuum isolation Isolation valves to switch vacuum and prevent process chamber cross-contamination
User Interface
User interface Full graphical interface with touch screen buttons and controls. In addition to displaying profiles, parameters, help screen and maintenance information are available
Profiles and profile logging Capability to store 100 user profiles (name, date, time, vacuum, current and polarity)
Dimensions and Communications
Chamber size 100 mm W x 100 mm H x 127 mm D (3.94" x 3.94" x 5")
Instrument size 336 mm H x 364 mm D (13.2" x 14.3")
Instrument weight 19.5 kg (42.9 lbs) (GloQube-D)
Pump (optional) 391 mm W x 127 mm D x 177 mm H (15.4" x 5" x 7")
Pump weight 16 kg (35.3 lbs)
Footprint with optional pump 366 mm W x 600 mm D x 336 mm H (14.4" x 23.6" x 13.2")
Power requirements 120 V 60 Hz, 15 A or 230 V 50 Hz, 10 A
Instrument power rating 100-240 V AC 60/50 Hz 700 VA including pump, IEC inlet
Optional pump power rating 115/230 V 60/50 Hz 450 W
Communication port Ethernet port for instrument software updates

Ordering Information

EMS-Glo-2 EMS GloQube, Dual chamber glow discharge system. Accessory kit, including: mains power lead, rotary pump power lead, oil mist filter and clamp, 750 mm long flexible stainless steel vacuum tube with clamps, fuses, glass vials, vial caps and sealing washers, needle (spare). Vacuum pump to be ordered separately. each 14,000.00 Add to Cart
Vacuum pumping
91003 5 m3/hr Pfeiffer DUO 6 two-stage rotary vacuum pump with oil mist filter each 2,600.00 Add to Cart
96001 Vacuum Pump Exhaust Filter including adapter (½" female NPT to ¾" BSPT with ¼" NPT plug) each 280.00 Add to Cart
Options, accessories and spares
EMS-Glo-11 Microscope Slide Tray each POR Quote
EMS-Glo-12 Glass Vial 10/pk POR Quote
EMS-Glo-13 Glass Vial Caps 3/pk POR Quote
EMS-Glo-14 Needle each POR Quote
EMS-Glo-15 Door Seal each POR Quote
EMS Grid Prep Holder, 36 Capacity
EMS Grid Prep Holder, 81 Capacity

arrow13EMS Grid Prep Holder

Electron Microscopy Sciences introduces unique preparation trays which may be used for staining and/or placing an evaporator for the coating of grids.

The trays have a M4 tapped hole in the center which allows for insertion of pin mount. They also have a screw pin in the middle that may be used as a handle for manipulating the trays.

They are made from medical grade aluminum and come in a plastic storage box.

Available in three sizes:

  1. 25 capacity: measures 1.4"(L) x 1.4"(W) x 0.250" Thick (35 mm x 35 mm x 6.4 mm)
  2. 36 capacity: measures 1.688"(L) x 1.688"(W) x 0.250" Thick (43 mm x 43 mm x 6 mm)
  3. 81 capacity: measures 2.563"(L) x 2.563"(W) x 0.250" Thick (65 mm x 65 mm x 6 mm)
71175-00 EMS Grid Prep Holder, 25 Capacity each 58.00 Add to Cart
71175-01 EMS Grid Prep Holder, 36 Capacity each 58.00 Add to Cart
71175-02 EMS Grid Prep Holder, 81 Capacity each 76.50 Add to Cart

Coaters and Replacement Parts arrow13arrow13