Cryo-Embedding
PolarChucks™
PolarChuck™ cryostat embedding chucks are made of anodized aluminum. Deep rectangular waffle grid or circular pattern creates a more stable and secure tissue mount.
| 70171-01 | PolarChuck™ L for Leica Cryostats, Waffle Grid, 30 mm | each | 60.00 | Add to Cart |
| 70171-02 | PolarChuck™ L for Leica Cryostats, Waffle Grid, 40 mm | each | 81.00 | Add to Cart |
| 70171-03 | PolarChuck™ Circular Pattern, 30mm Thin St | each | 60.00 | Add to Cart |
| 70171-04 | PolarChuck™ Circular Pattern, 40mm Thin St | each | 75.00 | Add to Cart |
| 70171-05 | PolarChuck™ A for Avantik/Microm Cryostats, Waffle Grid, 30 mm | each | 60.00 | Add to Cart |
| 70171-06 | PolarChuck™ A for Avantik/Microm Cryostats, Waffle Grid, 40 mm | each | 81.00 | Add to Cart |
Microtome Chucks
Aluminum Microtome Chucks with grooved surfaces for good specimen adhesion.
| EMS065 | Aluminum Microtome Chuck, Surface Diameter 45 mm with a 9.4 mm spigot | each | 123.25 | Add to Cart |
| EMS066 | Aluminum Microtome Chuck, Surface Diameter 26 mm with a 9.4 mm spigot | each | 107.10 | Add to Cart |
| EMS067 | Aluminum Barrel Microtome Chuck, Surface Diameter 22 mm with a 9.4 mm spigot | each | 54.40 | Add to Cart |
The Davidson® Cryocup® System
Streamline the work of frozen tissue preparation.
The Davidson® Cryocup® System works with your existing equipment, such as microtomes and cryostats. Compatibility includes Leica, Microm (Avantik), Sakura, and Thermo-Shandon equipment.
Benefits
- Maintains cold temperature for quick freezing.
- Facilitates perfect flattening of oriented tissue specimens during freezing. Samples can be produced quickly, ready for slicing with little or no facing required.
- Quickly fills in if your cryostat loses temperature to help prevent work interruption.
- Portable, space-saving, and economical. Use in the field or in a satellite lab.
How to use the Cryocup® System
Following this process can achieve a perfectly flattened specimen, already faced, mounted on a chuck, and ready for slicing, in under a minute:
- Place dyed specimen into a Cryocup®.
- Fill with OCT up to the ledge.
- Place a Cryo Chuck (or one you already have) on top of the specimen.
- Place the Cryocup® in the Cryo Holder and lower the cover onto the Cryocup®.
- Dip the Cryocup® into liquid nitrogen (10 seconds).
- Drain. Remove Cryocup®.
- Warm Cryocup® slightly to release tissue specimen.
Ordering Information
Base your size choice on the size of chuck you may already have with your equipment, or the size of specimens you typically process.
NOTE: BPI indicates compatibility with most Leica, Sakura, and old style Thermo-Shandon equipment.
Cryocup® Complete Systems
Include Holder, 3 Cryocups®, Nitrogen Reservoir, Water Basin. Chucks are not included.| Catalog # | System Size | Chuck Compatibility | Qty. | Price | |
| 61736-01 | 30mm | BPI/Leica 30mm | each | 575.00 | Add to Cart |
| 61736-02 | 30mm | Microm 30mm | each | 575.00 | Add to Cart |
| 61736-03 | 40mm | BPI 40mm | each | 600.00 | Add to Cart |
| 61736-04 | 40mm | Leica 40mm | each | 600.00 | Add to Cart |
| 61736-05 | 40mm | Microm 40mm | each | 600.00 | Add to Cart |
| 61736-06 | 50mm | BPI/Leica 50mm | each | 650.00 | Add to Cart |
Cryocup® Holders
Includes dipper, cover, and clip.| Catalog # | Holder Size | Compatibility | Qty. | Price | |
| 61736-07 | 30mm | BPI/Leica (S cover, S hole) | each | 275.00 | Add to Cart |
| 61736-08 | 30mm | Microm (S cover, S hole) | each | 275.00 | Add to Cart |
| 61736-09 | 40-50mm | BPI/Leica (L cover, L hole) | each | 295.00 | Add to Cart |
| 61736-10 | 40mm | Microm (L cover, L hole) | each | 290.00 | Add to Cart |
Cryocup®
| Catalog # | Cup Size | Chuck Compatibility | Qty. | Price | |
| 61736-11 | 30mm | BPI/Leica/Microm 30mm | each | 75.00 | Add to Cart |
| 61736-12 | 40mm | BPI/Leica 40mm | each | 85.00 | Add to Cart |
| 61736-13 | 40mm | Microm 40mm | each | 85.00 | Add to Cart |
| 61736-14 | 50mm | BPI 50mm | each | 105.00 | Add to Cart |
Cryocup® Chucks
| Catalog # | Chuck Size | Qty. | Price | |
| 61736-15 | 30mm | each | 55.00 | Add to Cart |
| 61736-16 | 40mm | each | 70.00 | Add to Cart |
| 61736-17 | 50mm | each | 85.00 | Add to Cart |
Precision Cryo-Embedding System
This section offers you a simple system of stainless steel well bars, chucks and freezing blocks, kept cold in cryostat, allow you to perform a variety of precision embedding techniques addressing the most difficult embedding tasks with ease and minimum time needed.
Speed
- 20 to 60 seconds to freeze (depending on the well size)
- Multiple samples handled quickly
- No monitoring necessary (for placement of heat extractor)
Precision
- Precise flat or on edge embedding
- Predictable orientation
- Liquid samples easily embedded
- No crush artifact caused by heat extractor weight
Reduced Tissue Waste
- Less trimming the result of flat plane of embedding
- Minute samples embedding in the same plane
Easy and Comfortable
- Less cryostat adjustments
- Comfortably preformed on a shelf just inside most cryostats
- Easy to used – and safe time
1. Embedding Wells
| 61710-18 | Embedding Bar, 4-Well of 18 x 18mm |  |
each | 280.00 | Add to Cart |
| 61710-24 | Embedding Bar, 4 well of 24 x 24 mm |  |
each | 280.00 | Add to Cart |
| 61710-30 | Embedding Bar, 3 well of 30 x 30 mm |  |
each | 280.00 | Add to Cart |
| 61712-34 | Embedding Bar, 2 wells of 30 & 24 mm |  |
each | 290.00 | Add to Cart |
| 61713-50 | Embedding Bar, 2 well of 50 x 30 mm completely fills a standard glass slide |  |
each | 280.00 | Add to Cart |
| 61714-20 | Embedding Bar, 20 x 20 mm, 9mm Deep Wells |  |
each | 280.00 | Add to Cart |
| 61715-24 | Embedding Bar, 24 x 24 mm Deep Wells |  |
each | 280.00 | Add to Cart |
| 61716-10 | Embedding Bar, 10 x10 mm Wells | |
each | 280.00 | Add to Cart |
2. Stainless Steel Chucks
These stainless steel chucks are designed to be used cold for rapid freezing, and to maximize the gripping power required to hold the embedded tissue block. The stem of the chuck provides the focal point for a sharp tap resulting in an easily release of the block from the well. These chucks fit many of the major brand cryostat and can be used in most cryostats with use of an adapter. These Chuck Adapters have stem measures 20mm L x 9mm diameter, and fit Leica and most Microm cryostats. We also offer adapters to use these chuck for other cryostats.
| 61720-28 | Small Chuck – Made of stainless steel, 28 mm square with gridded surface | each | 70.00 | Add to Cart |
| 61720-36 | Large Chuck – Made of stainless steel, 36 mm square with gridded surface | each | 80.00 | Add to Cart |
| 61721-55 | Extra Large Chuck – Stainless steel 55 x 36 mm rectangular shape | each | 90.00 | Add to Cart |
3. Stainless Steel Over Chucks Freezing Blocks
Thee stainless steel rectangular blocks are a useful tool for a number of tasks. They serve as a heat extractor, designed to fit over the stem of the chuck. The flat surfaces can also be used as an extra heat extractor when using conventional cryostat embedding technique. The freezing block also served as a dislodging tool. A light tap of the chuck stem cleaves the plane of adhesion holding of the formed block to the well. These blocks also serve as a convenient hand held flat freezing surface useful in gently cooling the tissue without the use of sprays and in plastering technique.
| 61725-01 | Over-Chuck Freezing Block | each | 28.00 | Add to Cart |
4. Chucks Bin
A sturdy stainless steel bin to store over-chuck freezing block while staying cold in cryostat. Removable cover for easy access the contents.
| 61725-50 | Chuck Storage Bin | each | 30.00 | Add to Cart |
5. Dispensing Slides
These thin transparent polyvinyl slides serve as a surface to precisely orient the tissue into the desired position and as a means to accurately transfer tissue to the embedding well floor. Tissue is applied face down to the end of the dispensing slide where it can be visualized from below and manipulated into position. The face that is visible will be laid onto the well floor and will ultimately be the embedded surface to be sectioned.
| 61727-08 | Dispensing Slide | 8/pk | 28.00 | Add to Cart |
6. Embedding Shelf
This removable shelf can be installed below the opening of the cryostat in the most convenient and location available. The selves can be made in various sizes to accommodate most instruments.
Also available two more models of 3 pieces shelf size 4 x 8" for temporary or permanently installation. One for Leica or Micron cryotats, and one for Shandon Cryostat
| 61728-01 | Embedding Shelf, size 4 x 8" | each | 100.00 | Add to Cart |
| 61728-LM | Embedding Shelf for Leica/Micron | each | 225.00 | Add to Cart |
| 61728-Sh | Embedding Shelf for Shandon | each | 210.00 | Add to Cart |
7. Chuck Adapter
This Chuck Adapter machined to fit Shandon and Ames / Tissue-Tek cryostats.
| 61731-01 | Chuck Adapter for Shandon | each | 110.00 | Add to Cart |
| 61732-01 | Chuck Adapter for Ames/Tissue-Tek | each | 110.00 | Add to Cart |
8. Frozen Section Brushes
These flat white boar bristle brush which is cut into an angle. This stiffness bristle will prevent the frozen section from curling through the brush. We offer a set of four brushes: two #1 with ⅜" angle cut and #2 with ¼" angle cut.
| 61735-01 | Frozen Section Brush | 4/set | 40.00 | Add to Cart |
Well Bar Sets
We offer these Well Bar Sets to make your ordering easier and to offer you a complete set for facilitate your work:
Well Bars Set 101
Includes: Three sizes well bars (61710-18, 61710-24, 61710-30); Six small chucks (6 x 61720-28); Four large chucks (4 x 61720-36); Four Over-Chuck Freezing Blocks (4 x 61725-01); One Chuck Bin (1 x 61725-50); 16 Dispensing Slide (61727-08); Cutting Board/Freezing Girdle (61733-01); 1 Elevated Freezing Block (61734-01); 1 Angle Embedding Forceps (78359-124); and accessories
| 61700-01 | Well Bars Set 101 | set | 2,100.00 | Add to Cart |
Well Bars Set 102
This set is dedicated for Ames / Tissue-Tek Cryostats.
Includes: Two sizes well bars (61710-18, 61710-24); Six small chucks (6 x 61720-28); Four large chucks, 36x36mm (4 x 61720-36); One Chuck Adapter (61732-01); Four Over-Chuck Freezing Blocks (4 x 61725-01); One Chuck Bin (1 x 61725-50); 16 Dispensing Slide (61727-08); Cutting Board/Freezing Girdle (61733-01); 1 Elevated Freezing Block (61734-01); 1 Angle Embedding Forceps (78359-124); and accessories
| 61701-01 | Well Bars Set 102 | set | 1,840.00 | Add to Cart |
Small Embedding Well Bar Set
Includes: One 18 mm Well Bar (61710-18); Four Small Chucks (61720-28); Two Over Chuck Freezing Blocks (61725-01); Dispensing Slides (61727-08); Accessories included.
| 61704-01 | Small Embedding Well Bar Set | 550 | 640.00 | Add to Cart |
Medium Embedding Well Bar Set
Includes: One 24 mm Well Bar (61710-24); Four Small Chucks (61720-28); Two Over Chuck Freezing Blocks (61725-01); Dispensing Slides (61727-08); Accessories included.
| 61704-02 | Medium Embedding Well Bar Set | set | 680.00 | Add to Cart |
Large Embedding Well Bar Set
Includes: One 30 mm Well Bar (61710-30); Four Small Chucks (61720-36); Two Over Chuck Freezing Blocks (61725-01); Dispensing Slides (61727-08); Accessories included.
| 61704-03 | Large Embedding Well Bar Set | set | 680.00 | Add to Cart |
24/30 mm Well Bar Set
Includes: One Well Bar with two wells 24 mm and two wells 30mm (61712-34); Two Large Chucks (61720-36); Two Small Chucks (61720-28); Two Over Chuck Freezing Blocks (61625-01); 8 Dispensing Slides (61727-08)
| 61704-04 | 24/30 mm Well Bar Set | set | 670.00 | Add to Cart |
50/30 mm Well Bar Set
Includes: One Well Bar 50 x 30 mm (61713-50); Two Large Chucks (61720-36) Two Small Chucks 55 x 36 mm (61721-55)
| 61704-05 | 50/30 Well Bar Set | set | 470.00 | Add to Cart |
20 x 20 mm Deep Well Bar Set
One 20 x 20 mm Deep Well Bar (61714-20); Two Small Chucks (61720-28); Two Over Chuck Freezing Blocks (61725-01); 8 Dispensing Slides (61727-08)
| 61704-06 | 20 x 20 mm Deep Well Bar Set | set | 640.00 | Add to Cart |
Freezing Griddle/ Elevated Freezing Block Set
Includes
Cutting board / Freezing griddle: The steel "freezing griddle" serves as a freezing surface on which to prepare frozen blocks when used in conjunction with the elevated freezing block. The small attached cutting board serves as a cold surface to cut the frozen block while maintaining the freezing temperature. This simple piece of apparatus is kept cold in the cryostat and is removed to the work bench only during the process of cutting the frozen block. As sections are cut, they are kept cold on the freezing griddle.
Elevated Freezing Block:This steel block is kept cold in the cryostat and in used in conjunction with the freezing griddle. The two broad sides of the block are equipped with feet measuring 3.0 mm on one side and 4.5 mm on the other. The feet create a space between the two steel bars in which the frozen blocks are prepared. Additional elevating bars are supplied to further elevate the block when preparing very thick portion of tissue.
Epoxy Coated Forceps
Putty knife and extra elevating bars
| 61706-01 | Freezing Griddle/ Elevated Freezing Block Set | each | 320.00 | Add to Cart |
