Reagents for Histology: Fixing, Clearing, and Dehydrating

EMS is now offering a complete line of reagents for Histopathology: The highest quality for Fixing, Dehydrating/Clearing and Staining.

Acetate Buffer

RT	11482-42	Acetate Buffer, 0.2M, pH 4.2		500 ml	23.00	Add to Cart
RT	11482-42-1L	Acetate Buffer, 0.2M, pH 4.2		1 L	46.00	Add to Cart
RT	11482-50	Acetate Buffer, 0.2M, pH 5.0		500 ml	23.00	Add to Cart
RT	11482-50-1L	Acetate Buffer, 0.2M, pH 5.0		1 L	46.00	Add to Cart
RT	11482-56	Acetate Buffer, 0.2M, pH 5.6		500 ml	23.00	Add to Cart
RT	11482-56-1L	Acetate Buffer, 0.2M, pH 5.6		1 L	46.00	Add to Cart

Acetic Acid, Glacial, Reagent A.C.S

C2H4O2 F.W. 60.05 CAS 64-19-7

Assay min

99.7%

RT	10040	Acetic Acid, Glacial	500 ml	59.00	Add to Cart
RT	10041	Acetic Acid, Glacial	2.5 L	120.00	Add to Cart

Acetic Acid, Aqueous Solution

Prepared from acetic acid glacial with de-ionized water.

RT	10042-02	Acetic Acid, 0.2% (v/v) Solution		500 ml	19.00	Add to Cart
RT	10042-05	Acetic Acid, 0.5% (v/v) Solution		500 ml	20.00	Add to Cart
RT	10042-10	Acetic Acid, 1.0% (v/v) Solution		500 ml	22.00	Add to Cart
RT	10042-50	Acetic Acid, 5.0% (v/v) Solution		500 ml	30.00	Add to Cart

Acetone, Reagent, A.C.S.

CH₃COCH₃   F.W. 58.08   CAS #67-64-1

A.C.S. Specifications

Assay		Min 99.5%
Aldehyde (as HCHO)		0.002%
Color		Max. APHA 10
Density (G/ml) at 25°C		Max. 0.7857
Titratable Acid		0.0003 meq/g
Titratable Base		0.0006 meq/g
Isopropyl Alchohol		0.05%
Methanol (CH3OH)		0.05%
Residue after Evaporation		0.001%
Solubilty in water		To pass test
Substances Reducing Permanganate		To pass test
Water		0.5%

RT	10000		1 qt. (946 ml)	15.00	Add to Cart
RT	10010		4 x 1 qt.	52.00	Add to Cart
RT	10012		250 ml	8.25	Add to Cart
RT	10014		4 x 250 ml	28.00	Add to Cart

Acetone: Alcohol, 1:1 & Buffered Acetone

RT	10017	Acetone: Alcohol, 1:1		500 ml	21.00	Add to Cart
RT	10018	Buffered Acetone Fixative		500 ml	25.00	Add to Cart

Acid Alcohols

An acidified alcohol solution for the differentiation of regressive stains.

RT	26071-01	Acid Alcohol, 0.5% HCl in 70% Alcohol		1 L	37.00	Add to Cart
RT	26071-04	Acid Alcohol, 0.5% HCl in 70% Alcohol		4 x 1 L	138.00	Add to Cart
RT	26072-01	Acid Alcohol, 1% HCl in 70% Alcohol		1 L	33.00	Add to Cart
RT	26072-04	Acid Alcohol, 1% HCl in 70% Alcohol		4 x 1 L	120.00	Add to Cart
RT	26073-01	Acid Alcohol, 1% HCl in 95% Alcohol		1 L	43.00	Add to Cart
RT	26073-04	Acid Alcohol, 1% HCl in 95% Alcohol		4 x 1 L	160.00	Add to Cart

Ethyl Alcohol, Specially Denatured

C₂H₅OH   F.W. 46.07   CAS #64-17-5

Blended 95% Ethanol and 5% Methanol.

Suitable for all routine histology/cytology preparations.

Specifications

Evaporation Residue		5ppm max.
Water		0.1%
Filtered SDA-3A		95%

RT	15050		1 qt	25.00	Add to Cart
RT	15051		4 x 1 qt	82.00	Add to Cart

Ethyl Alcohol, Anhydrous

(Ethanol) – 200 Proof
CH₃CH₂OH   F.W. 46.07   CAS # 64-17-5

Specifications

Specific Gravity	<0.7942
Purity	>99.9%
Acidity	<0.002%
Water (Karl Fisher)	<0.1%
Non-Volatile Matter	<0.0025%

RT	15055		450 ml	22.00*	Add to Cart
RT	15056		4 x 450 ml	72.00*	Add to Cart
RT	15058		1 liter	36.00	Add to Cart

*Tax has already been paid

Alkaline Alcohol

RT	10380	10% NH4OH in 85.5% Alcohol		500 ml	24.00	Add to Cart
RT	10381	1.6% NaCl in 80% Alcohol		500 ml	39.00	Add to Cart
RT	10382	0.2% KOH in 80% Alcohol		500 ml	40.00	Add to Cart

Ammonium Hydroxide, 28% Reagent, A.C.S.

NH₄OH   F.W. 35.05   CAS #1336-21-6 Specific Gravity: 0.91.

Assay

28-30%

Specifications

Residue After Ignition		0.002%
Carbon Dioxide		0.002%
Chloride		0.5 ppm
Phosphate		2 ppm
Sulfate		2 ppm
Heavy Metals		0.5 ppm
Iron		0.2 ppm

RT	10600		450 ml	19.00	Add to Cart
RT	10601		4 x 450 ml	70.00	Add to Cart

Amphibian Ringer Solution

Ingredients: NaCl; KCl; CaCl₂·6H₂O and Distilled water.

RT	11762-05	Amphibian Ringer Solution	500 ml	25.00	Add to Cart
RT	11762-10	Amphibian Ringer Solution	1 L	35.00	Add to Cart

B-5 Fixative

For lymph nodes.

RT	64122-05	B-5 Fixative	500 ml	64.00	Add to Cart
RT	64122-06	B-5 Fixative	1 lt	120.00	Add to Cart
RT	64122-10	B-5 Fixative	4 lt	450.00	Add to Cart

Bouin's Solution

Bouin's solution can be used as a fixation and a staining fluid. Bouin's fixative is excellent for use on biopsy specimens of the gastrointestinal tract. Tissue from the endocrine system are well fixed and many antibodies react well with tissue fixed in Bouin's. Bouin's solution may be used as a routine fixative or a staining solution, but should not be used as a preservative for tissue. Tissue cannot be held in Bouin's solution for extended periods. The maximum fixation time in this solution should be less than 48 hours and should be transferred to alcohol. Packaged in leak-proof histo-containers, prefilled and ready-to-use.

Formulated with Picric Acid saturated aqueous solution 75%, Formalin (40% aqueous formaldehyde) 25% and Glacial Acetic acid 5% in de-ionized water.

RT	15990-01	Bouin's Fluid	1 L	47.00	Add to Cart
RT	15990-04	Bouin's Fluid	4 L	76.00	Add to Cart
RT	15990-04-5G	Bouin's Fluid	5 Gal.	175.00	Add to Cart
RT	15990-10	Bouin's Fluid Pre-filled cups	50x10 ml/cs	83.00	Add to Cart
RT	15990-20	Bouin's Fluid Pre-filled cups	50x20 ml/cs	103.00	Add to Cart
RT	15990-30	Bouin's Fluid Pre-filled cups	20x30 ml/cs	47.00	Add to Cart

Buffers

Antibody Dilution Buffer with Immunoglobulin-Free BSA

This ready-to-use Antibody Dilution Buffer is designed for the dilution of antibodies in all immunoassays, including immunofluorescence, IHC, ELISA, WB. This buffer contains immunoglobulin free BSA and does not contain phosphate, sodium azide, or mercury preservatives. It can be used for the dilution of all antibodies, including peroxidase, and antibodies to phophoproteins. However, it is not suitable for dilution of antibodies to S100 proteins. This buffer contains green food color.

Reagent Supplied: Ready to use buffer.

Application: Dilute antibodies, primary or secondary as recommended in the protocol by the manufacturer of these products.

Storage: 2-8°C. Diluted antibodies can be stored at 2-8°C for several weeks, depending upon the stability of antibodies.

2–8°C	25886-01	Antibody Dilution Buffer, free BSA	100 ml	62.00	Add to Cart
2–8°C	25886-05	Antibody Dilution Buffer, free BSA	500 ml	176.00	Add to Cart
	1227SK	Antigen Preserve Solution VII	1 L	154.00	Add to Cart

Borate Buffer

RT	11455-78	Borate Buffer, pH 7.8		500 ml	33.00	Add to Cart
RT	11455-82	Borate Buffer, pH 8.2		500 ml	34.00	Add to Cart
RT	11455-90	Borate Buffer, pH 9.0		500 ml	34.00	Add to Cart

Citrate Buffer

Citric Acid and Sodium Citrate, 0.1M. pH range 4.6-6.2.

0-5°C	11490	Citrate Buffer, 0.1M	225 ml	18.00	Add to Cart
0-5°C	11492	Citrate Buffer, 0.1M	500 ml	23.00	Add to Cart
0-5°C	11492-1L	Citrate Buffer, 0.1M	1 L	42.00	Add to Cart

Michel's Buffer

RT	18041-05	Michel's Buffer	500 ml	22.00	Add to Cart
RT	18041-10	Michel's Buffer	1 L	29.00	Add to Cart
RT	18041-10-1G	Michel's Buffer	1 Gal.	95.00	Add to Cart
RT	18041-10-5G	Michel's Buffer	5 Gal.	450.00	Add to Cart

Millonig's Phosphate Buffer

Millonig (1964); Karlsson and Schultz (1965)
Ingredients:
Sodium Phosphate, monobasic (NaH₂PO₄xH₂O)
Sodium Phosphate, dibasic (Na₂HPO₄xH₂O)
0.5% Sodium Chloride (NaCl)
Shipping Conditions: Ambient Temperature

The pH of this buffer is 7.4 (0.1M); the osmolarity is 440 mosmols, so this buffer is hypertonic to most body fluids. Millonig recommended to use this buffer for the fixation of very hydrated tissues with osmium tetroxide. For marine organisms, a higher concentration of sodium chloride (3%) is recommended. We supply it in a double strength (0.2M), pH 7.4. Dilution is needed before use.

0-5°C	11582-05	Millonig's Phosphate Buffer 0.2M	500 ml	23.00	Add to Cart
0-5°C	11582-10	Millonig's Phosphate Buffer 0.2M	1 L	32.00	Add to Cart
0-5°C	11582-10-1G	Millonig's Phosphate Buffer 0.2M	1 Gal.	120.00	Add to Cart
0-5°C	11582-10-5G	Millonig's Phosphate Buffer 0.2M	5 Gal.	525.00	Add to Cart

PHEM Buffer

EMS is proud to announce and release of our newest Buffer for EM PHEM.

PHEM buffer has been used primarily for tissues and cell cultures being processed for immunocytochemical studies.  Most antigens, especially intra cellular ones, stain better using PHEM than those processed using PBS, it also has a more limited effect on biochemical reactions and enzymes. Very good preservation of cellular ultrastructure, notably microtubules, is also an advantage of PHEM use.

References

1. Schliwa, Manfred, and Jonathan Van Blerkom. "Structural interaction of cytoskeletal components." The Journal of Cell Biology 90.1 (1981): 222-235.
2. Jacqueline Montanaro: Improved ultrastructure of marine invertebrates using non-toxic buffers

Academic Editor: James Reimer, published online March 31, 2016

2-8°C	11162	PHEM Buffer, 0.1M	225ml	42.00	Add to Cart
2-8°C	11163	PHEM Buffer, 0.1M	500ml	74.00	Add to Cart
2-8°C	11164	PHEM Buffer, 0.2M	225ml	48.00	Add to Cart
2-8°C	11165	PHEM Buffer, 0.2M	500ml	82.00	Add to Cart

Phosphate Buffer, 0.1M*

Prepared from Sodium Phosphate Monobasic Hydrate and Sodium Phosphate Dibasic Heptahydrate. Non-Sterile. Meant for research purposes only.

0-5°C	19340-60	Phosphate Buffer, pH 6.0		1 L	40.00	Add to Cart
0-5°C	19340-60-1G	Phosphate Buffer, pH 6.0		1 Gal.	104.00	Add to Cart
0-5°C	19340-65	Phosphate Buffer, pH 6.5		1 L	40.00	Add to Cart
0-5°C	19340-65-1G	Phosphate Buffer, pH 6.5		1 Gal.	104.00	Add to Cart
0-5°C	19340-70	Phosphate Buffer, pH 7.0		1 L	40.00	Add to Cart
0-5°C	19340-70-1G	Phosphate Buffer, pH 7.0		1 Gal.	104.00	Add to Cart
0-5°C	19340-72	Phosphate Buffer, pH 7.2		1 L	40.00	Add to Cart
0-5°C	19340-72-1G	Phosphate Buffer, pH 7.2		1 Gal.	104.00	Add to Cart

*Meant for biological research only. Not to be used for medical or any other purposes.

Phosphate Buffer Saline (PBS)

PBS is commonly used in biochemistry. It is a salty solution containing sodium chloride, potassium chloride, sodium phosphate and potassium phosphate. PBS is isotonic and non-toxic to cells. 1X PBS final concentration is 8.0 g/L NaCl, 0.2 g/L KCl, 1.42 g/L Na₂HPO₄, 0.24 g/L KH₂PO₄ and pH 7.4.

0-5°C	19342-10	Phosphate Buffered Saline, 10X		1 L	32.00	Add to Cart
0-5°C	19242-40	Phosphate Buffered Saline, 10X		1 gal	98.00	Add to Cart
0-5°C	19343-10	Phosphate Buffered Saline, 2X		1 L	28.00	Add to Cart
0-5°C	19343-40	Phosphate Buffered Saline, 2X		1 gal	88.00	Add to Cart
0-5°C	19344-10	Phosphate Buffered Saline, 1X		1 L	24.00	Add to Cart
0-5°C	19344-20	Phosphate Buffered Saline, 1X		1 gal	76.00	Add to Cart

Sorensen's Phosphate Buffer

A Phosphate buffered mixed salt prepared from sodium phosphate monobasic and sodium phosphate dibasic.

0-5°C	11600-10	Sorensens Phosphate Buffer, 0.2M, pH 7.2		1 L	32.00	Add to Cart
0-5°C	11600-40	Sorensens Phosphate Buffer, 0.2M, pH 7.2		4 L	110.00	Add to Cart
0-5°C	11601-10	Sorensens Phosphate Buffer, 0.2M, pH 7.4		1 L	33.00	Add to Cart
0-5°C	11601-40	Sorensens Phosphate Buffer, 0.2M, pH 7.4		4 L	110.00	Add to Cart

Tris, (hydroxymethyl) aminomethane maleate

Tris Maleate Buffer 0.2M pH range 6.4-8.0

0-5°C	11730	Tris Maleate Buffer 0.2M pH range 6.4-8.0	250 ml	23.00	Add to Cart
0-5°C	11740	Tris Maleate Buffer 0.2M pH range 6.4-8.0	500 ml	30.00	Add to Cart
0-5°C	11740-1L	Tris Maleate Buffer 0.2M pH range 6.4-8.0	1 L	50.00	Add to Cart
0-5°C	11740-1G	Tris Maleate Buffer 0.2M pH range 6.4-8.0	1 Gal.	175.00	Add to Cart

Tris Buffer 0.2M pH 8

RT	11730-05	Tris Buffer 0.2M pH 8.0	250 ml	23.00	Add to Cart
RT	11730-06	Tris Buffer 0.2M pH 8.0	500 ml	30.00	Add to Cart
RT	11730-06-1L	Tris Buffer 0.2M pH 8.0	1 L	50.00	Add to Cart
RT	11730-06-1G	Tris Buffer 0.2M pH 8.0	1 Gal.	175.00	Add to Cart

Tris Buffered Saline pH 7.4

RT	11730-08	Tris Buffered Saline pH 7.4	250 ml	23.00	Add to Cart
RT	11730-09	Tris Buffered Saline pH 7.4	500 ml	30.00	Add to Cart
RT	11730-09-1L	Tris Buffered Saline pH 7.4	1 L	50.00	Add to Cart
RT	11730-09-1G	Tris Buffered Saline pH 7.4	1 Gal.	175.00	Add to Cart

Tris HCL Buffer 1M pH 6-8

RT	11730-12	Tris HCL Buffer 1M pH range 6-8	250 ml	23.00	Add to Cart
RT	11730-15	Tris HCL Buffer 1M pH range 6-8	500 ml	30.00	Add to Cart
RT	11730-15-1L	Tris HCL Buffer 1M pH range 6-8	1 L	50.00	Add to Cart
RT	11730-15-1G	Tris HCL Buffer 1M pH range 6-8	1 Gal.	175.00	Add to Cart

Tris HCL Buffer pH range 7.2 - 9.0

RT	11730-20	Tris HCL Buffer pH range 7.2-9.0	250 ml	23.00	Add to Cart
RT	11730-25	Tris HCL Buffer pH range 7.2-9.0	500 ml	30.00	Add to Cart
RT	11730-25-1L	Tris HCL Buffer pH range 7.2-9.0	1 L	50.00	Add to Cart
RT	11730-25-1G	Tris HCL Buffer pH range 7.2-9.0	1 Gal.	175.00	Add to Cart

Universal Immunoassay Buffer™, 10X

Immunoassay buffer is used as a 1X in all immunoassays (immunofluorescence, IHC, ELISA, WB etc.) as a washing buffer and it can be used as a dilution buffer for antibodies; however it does not contain any carrier proteins.

This buffer also does not contain any phosphate, sodium azide or mercury as preservatives and can be used for dilution of all antibodies, including peroxidase, antibodies to phosphoproteins. It is not suitable for dilution of antibodies to S100 proteins.

Reagent Supplied: 10X Buffer.

Application: Dilute this 10X buffer 10 times with DI water. (90 ml water + 10 ml this buffer – mixed well). Wash 3-5 times with this buffer in between the steps of your immunoassay protocol.

Storage: Room temperature.

RT	25880-01	Universal Immunoassay Buffer, 10X	100 ml	60.00	Add to Cart
RT	25880-10	Universal Immunoassay Buffer, 10X	1 L	160.00	Add to Cart

Universal Antibody Dilution Buffer™

This ready-to-use Universal Antibody Dilution Buffer is designed for dilution of antibodies in all immunoassays, including immunofluorescence, IHC, ELISA, WB. This buffer does not contain any mammalian proteins, phosphate, sodium azide, or mercury preservatives and can be used for dilution of all antibodies, including peroxidase, and antibodies to phophoproteins. However, it is not suitable for dilution of antibodies to S100 proteins.

Reagent Supplied: Ready to use buffer.

Application: Dilute antibodies, primary or secondary as recommended in the protocol by the manufacturer of these products.

Storage: 2-8°C. Diluted antibodies can be stored at 2-8°C for several weeks, depending upon the stability of antibodies.

2-8°C	25885-01	Universal Antibody Dilution Buffer	100 ml	60.00	Add to Cart
2-8°C	25885-05	Universal Antibody Dilution Buffer	500 ml	165.00	Add to Cart

n-Butyl Alcohol, Reagent, A.C.S.

(Butanol)
CH₃CH₂CH₂CH₂OH   F.W. 74.12   CAS # 71-36-3

Specific Gravity:		0.81
Assay		99.4%
Residue after evaporation		0.005%
Titratable acid		0.0008meq/g
Butyl Ether		0.2%
Water		0.1%

RT	11920		450 ml	30.00	Add to Cart
RT	11921		4x450 ml	104.00	Add to Cart

Carnoy's Fixatives

Stock solution.

RT	64130-05	Carnoy's Fluid		500 ml	26.00	Add to Cart
RT	64130-50	Carnoy's Fluid Without Chloroform		500 ml	24.00	Add to Cart

Cedar Wood Oil

CAS #8000-27-9 Refractive Index @ 20°C 1.504+/-.002

For clearing.

RT

12420

100 ml

66.00

Add to Cart

Chloroform, Reagent, A.C.S.

CHCl₃   FW 119.38   CAS #67-66-3

A.C.S. Specifications

Color (APHA)		10
Density @ 25°C		1.471-1.476
Residue after Evaporation		0.001%
Acetone & Aldehyde (about 0.00%)		To Pass Test
Acid & Chloride		To Pass Test
Free Chlorine (Cl)		To Pass Test
Lead (Pb)		0.05ppm
Subs. darkened by H2SO4		To Pass Test
Suitability for Dithizone Test		To Pass Test

RT	12540		450 ml	20.00	Add to Cart
RT	12541		4x450 ml	78.00	Add to Cart

Chromic Acid, Reagent, Crystals, A.C.S.

CrO₃   FW 99.99   CAS #1333-82-0

A.C.S. Specifications

Chloride (Cl)		0.005%
Insoluble Matter		0.01%
Nitrate (NO3)		0.05%
Sodium (Na)		0.2%
Iron, Aluminum, Barium		0.03%
Sulfate (SO4)		0.05%
Assay		98%

RT

12555

100g

45.00

Add to Cart

Chromic Acid 4% Aqueous Solution

RT	12557	Chromic Acid 4% Aqueous Solution		500 ml	30.00	Add to Cart
RT	12558	Chromic Acid 5% Aqueous Solution		500 ml	34.00	Add to Cart

Clearing Solvent Xylene Substitutes

These clearing solvents offer Histology and Cytology laboratories a safer alternative to xylene.

• Contains no xylene or toluene
• No citrus odor
• For use in manual and automated systems

RT

64129-10

Clearing Solvent

1 gal

52.00

Add to Cart

RDO Rapid Decalcifier

RDO and RDO Gold were developed with the basic premise of making your work routine quicker and easier without sacraficing the quality and integrity of your samples.

RDO and RDO Gold are remarkably effective multipurpose decacifiers that can be tailored to suit your specific lab routine.

Both RDO and RDO Gold

• Offer crisp nuclear staining and definition
• Give distinguishable chromatin
• Show great surface decalcification
• Safe enough to be disposed down to lab sink to drain

How is RDO Gold different from the original RDO

• RDO Gold is designated for those biopsies which contain minimal amounts of calcium
• No filtration is required with RDO Gold
• RDO Gold gives stellar morphological representation of bone biopsies
• There is no folding of tissues with RDO Gold
• RDO Gold is clear and non-precipitate

Both RDO and RDO Gold are

• Far Superior to other Decalcifiers
• Great Cellular Differentiation
• Safe – Non Toxic – Non-Corrosive
• Dilutable
• Microwavable
• Superb Staining Quality
• Biodegradable
• Translucent
• Available in Wide Mouth Liter Bottle

References

1. Gayle Callis et al. "Dacalcification of Bone: Literature Review and Practical Study of Various Decalcifying Agents, Methods, and Their Effects on Bone Histology". The J. of Histology, Vol. 21, No. 1, March 1998
2. Sherley A. Powell, HT (ASCP) HTL. "Method to Facilitate Sectioning Incompletely Decalcified Bone". Mercer University, School of Medicine. Microtime Georgia, State Newsletter
3. Becky Scholes, HTL, MT (ASCP).:Bone Marrow Biopsies". H.I.S.T.O. Iowa State Newsletter

RT	64143-01	RDO Decalcifier		1 L	26.00	Add to Cart
RT	64143-06	RDO Decalcifier		6 x 1 L/cs	126.00	Add to Cart
RT	64143-40	RDO Decalcifier		1 gal	56.00	Add to Cart
RT	64143-44	RDO Decalcifier		4 x 1 gal/cs	195.00	Add to Cart
RT	64144-01	RDO – Gold Decalcifier		1 L	44.00	Add to Cart
RT	64144-06	RDO – Gold Decalcifier		6 x 1 L/cs	245.00	Add to Cart
RT	64144-40	RDO – Gold Decalcifier		1 gal	84.00	Add to Cart
RT	64144-44	RDO – Gold Decalcifier		4 x 1 gal/cs	299.00	Add to Cart

Technical Tips and Articles

RDO Rapid Decalcifier

Animal Tissue Techniques
Parafin and Plastic Techniques for Preparation of Bone Marrow Specimen
Bone Marrow Biopsies

Decalcifier

• Ready-to-use
• For hard specimens
• Protects cellular structure
• Minimal interference with subsequent staining
• For use with human and animal calcified tissue, bone, cartilage and other hard specimens

RT	64147-01	Decalcifier	1 L	32.00	Add to Cart
RT	64147-06	Decalcifier	6 x 1 L	138.00	Add to Cart
RT	64147-10	Decalcifier	10 L	179.00	Add to Cart

Deionized Water, Reagent Grade A.C.S.

H₂O F.W. 18.02 CAS #7732-18-5
EMS Reagent Grade Water is typically prepared at 18 megohm/cm specific resistance using a reverse osmosis, mixed deionization, activated filtration and final filtration at 0.2 microns.

Color (APHA)		<+/-5
Bacteriological purity		0 CFUs/L
Residue after evaporation		10ppm
Coliform		negative

RT	22800-01	Deionized Water	1 gal	12.00	Add to Cart
RT	22800-05	Deionized Water	5 gal	50.00	Add to Cart

EMS Rapid Pro Processing System

A Revolutionary Rapid Tissue Processing System

Tissue processing has not changed in more than a century... until now. The EMS Rapid Pro Processing System can cut total processing time to as little as 46 minutes.

Advantages over traditional processing

• Speed – no other processing chemicals will deliver results this fast
• Increased throughput
• Suitable for both fresh and frozen sections
• Catalyzed versions of standard processing chemicals eliminate the need for new safety features, storage, or learning curves
• Effective for manual or automatic tissue processing
• Compatible with existing tissue processing equipment
• "Results While You Wait" option for patients

For more staining protocols, see the Technical Data Sheet.

A proven and reliable method for obtaining rapid results in tissue processing

Process	Standard Processing Time	EMS Rapid Pro Processing Time
Formalin	½ hr - 1 hr	3 min
70% Ethanol	1 hr	3 min
95% Ethanol	1 hr	3 min
98% Ethanol	1 hr	3 min
90% Xylenes	½ hr - 1 hr	3 min
98% Ethanol	1 hr	3 min
90% Xylenes	½ hr - 1 hr	3 min
Paraffin	2 - 3 hrs	25 min
Total Processing TIme	7½ - 10 hrs	46 min

Ordering

26750-10	EMS Rapid ProKit		kit	36.00	Add to Cart
26750-01	EMS Rapid Pro Solution 1		250 ml	12.00	Add to Cart
26750-02	EMS Rapid Pro Solution 2		250 ml	11.00	Add to Cart
26750-03	EMS Rapid Pro Solution 3		250 ml	4.00	Add to Cart
26750-04	EMS Rapid Pro Solution 4		250 ml	4.00	Add to Cart
26750-05	EMS Rapid Pro Solution 5		250 ml	9.00	Add to Cart

Ferric Ammonium Sulfate

RT	15505-40	Ferric Ammonium Sulfate, 4% Aqueous		500 ml	31s.00	Add to Cart
RT	15505-50	Ferric Ammonium Sulfate, 5% Aqueous		500 ml	32.50	Add to Cart

Ferric Chloride, Hexahydrate, Reagent, A.C.S.

FeCl₃·6H₂O   F.W. 270.30   CAS #13478-10-9

Specifications

Assay	97.0-102%
Insoluble Matters.	0.01%
Nitrate (about 0.001%)	To Pass Test
Phosphorous Compound	0.01%
Sulfate	0.01%
Arsenic	0.002%
Copper	0.003%
Zinc	0.003%

References for use

Membrane stains

• Basic, G., Berwick et al (1963). Hale stain for sialic acid containing mucins. J. Cell Biol., 19:223
• Gasic, G., Berwick, et al (1968). Positive and Negative colloidal iron as cell surface electron stains. Lab. Invest., 18:63
• Blanquet, P.R. and Loiez, A. (1974). Colloidal iron used at pHs lower than 1 as electron stain for surface proteins. J. Histochem. Cytochem., 22:368
• Matukafs, V.J., Panner et al (1967). Studies on ultrastructural identification and distribution of protein-polysaccharides in cartilage matrix. J. Cell Biol., 32:365
• Benedetti, E.L. and Emmelot, P. (1967). Studies on plasma membranes IV. The Ultrastructural localization and content of sialic acid in plasma membranes isolated from rat liver and hepatoma. J. Cell Sci., 2:499
• Rowley, J.R. (1971). Resolution of channels in the exine by translocation of colloidal iron. Pro. 29th Ann. EMSA Meet., p.352. Claitor's Pub. Division, Baton Rouge, LA
• Nicolson, G.L. (1973). Anionic sites of human erythrocyte membranes.I. Effects of trypsin, phospholipase C, and pH on the topography of bound positively charged colloidal particles. J. Cell Biol., 57:373
• Hendy, R. (1971). Electron microscopy of lipofucsin pigment stained by the Schmorl and Fontana technique. Histochemie, 26:311

RT

15510

Ferric Chloride, Hexahydrate, Reagent

500g

45.00

Add to Cart

Ferric Chloride 10%, Aqueous Solution

RT	15512-02	Ferric Chloride 2% Aqueous		500 ml	37.00	Add to Cart
RT	15512-10	Ferric Chloride 10% Aqueous		500 ml	37.00	Add to Cart

Fixatives

B-5 Fixative

For lymph nodes.

RT	64122-05	B-5 Fixative	500 ml	64.00	Add to Cart
RT	64122-06	B-5 Fixative	1 L	120.00	Add to Cart
RT	64122-10	B-5 Fixative	4 L	450.00	Add to Cart

Bouin's Solution

Bouin's solution can be used as a fixation and a staining fluid. Bouin's fixative is excellent for use on biopsy specimens of the gastrointestinal tract. Tissue from the endocrine system are well fixed and many antibodies react well with tissue fixed in Bouin's. Bouin's solution may be used as a routine fixative or a staining solution, but should not be used as a preservative for tissue. Tissue cannot be held in Bouin's solution for extended periods. The maximum fixation time in this solution should be less than 48 hours and should be transferred to alcohol.

Packaged in leak-proof histo-containers, prefilled and ready-to-use.

Formulated with Picric Acid saturated aqueous solution 75%, Formalin (40% aqueous formaldehyde) 25% and Glacial Acetic acid 5% in de-ionized water.

RT	15990-01	Bouin's Fluid	1 L	47.00	Add to Cart
RT	15990-04	Bouin's Fluid	4 L	163.00	Add to Cart

Bouin's Fluid in Pre-filled cups.

RT	15990-10	Bouin's Fluid	50 x 10 ml/cs	83.00	Add to Cart
RT	15990-20	Bouin's Fluid	50 x 20 ml/cs	103.00	Add to Cart
RT	15990-30	Bouin's Fluid	20 x 30 ml/cs	47.00	Add to Cart

Brasil Fixative

Ethanol, Picric Acid, Formalin, Acetic Acid, Glacial.
This fixative is most popular when glycogen is the target. The solution is similar to Bouins Solution (alcoholic) offering quick hardening and good staining with acid dyes.

RT	15995-10	Brasil Fixative	10x10 ml	42.00	Add to Cart
RT	15995-20	Brasil Fixative	100 ml	42.00	Add to Cart
RT	15995-20-1L	Brasil Fixative	1 L	77.00	Add to Cart
RT	15995-20-1G	Brasil Fixative	1 Gal.	132.00	Add to Cart
RT	15995-20-5G	Brasil Fixative	5 Gal.	475.00	Add to Cart

Buffered Ethanol Fixative, 70% BE-70

EMS is proud to introduce this ready-to-use new fixative designed to offer the latest preservation of structural integrity with good immunostaining results. This fixative is a phosphate buffered ethanol (BE-70) with the addition of glycerol and glacial acetic acid.
Reference: Journal of Histochemistry & Cytochemistry 2017, Vol 65(4) p. 251-252

RT	15988	Buffered Ethanol Fixative	1 L	70.00	Add to Cart
RT	15988-1G	Buffered Ethanol Fixative	1 Gal.	225.00	Add to Cart

Carnoy's Fixatives

Stock solution.

RT	64130-05	Carnoy's Fluid		500 ml	26.00	Add to Cart
RT	64130-50	Carnoy's Fluid Without Chloroform		500 ml	24.00	Add to Cart

Carson's Phosphate Buffered Formalin

Millonig's phosphate buffered formalin as modified by Carson, Martin and Lynn, by using 4% formalin in 0.1M sodium phosphate monobasic with sodium hydroxide to pH 7.2.

RT	15732-05	Carson's Phosphate Buffered Formalin	500 ml	20.00	Add to Cart
RT	15732-10S	Carson's Phosphate Buffered Formalin	1 L	32.00	Add to Cart
RT	15732-10S-1G	Carson's Phosphate Buffered Formalin	1 Gal.	52.00	Add to Cart

Carson's Phosphate Buffered Paraformaldehyde

For some tissues, the use of formalin (contains 10 – 15% Methanol) is not suitable, we offer the alternative formaldehyde (prepared from paraformaldehyde – contains no methanol) for your convenience. Contains 4% paraformaldehyde.

RT	15733-05	Carson's Phosphate Buffered Paraformaldehyde	500 ml	23.00	Add to Cart
RT	15733-10	Carson's Phosphate Buffered Paraformaldehyde	1 L	37.00	Add to Cart
RT	15733-10-1G	Carson's Phosphate Buffered Paraformaldehyde	1 Gal.	59.00	Add to Cart

CytoPrep^™ Fixative

CytoPrep Fixative forms a clear protective coating over specimens while fixing cells. It is ideal for cytological smears and it dries within 4 to 6 minutes. Good for safe storage and transportation of slides. It is water soluble and contains no chlorofluorocarbons. Comes in a 4 oz. spray can.

RT

64145-01

CytoPrep Fixative

4 oz

40.00

Add to Cart

CytoFix

Cytology fixative spray designed for slide based cytology specimens for cellular preservation and transport.

Quickly spray a fine mist over the slide (covering the entire specimen) for cellular fixation and reduce air drying of the material.

CytoFix forms a thin, tough, clear protective coating over the specimen with a water and alcohol solution of Polyethylene glycol for cellular fixation and does not require rehydration by the laboratory prior to staining. Allows safe storing or transporting of slides.

Allow specimens to dry before sending to lab or for storage.

• Dries in 4 to 6 minutes
• Water soluble
• Won't cloud or obscure specimen
• Contains no chlorofluorocarbons

Available in pump bottle or aerosol can.

22805-01	CytoFix (Non-Aerosol)	100 ml	14.50	Add to Cart
22805-02	CytoFix Can	4 oz	14.50	Add to Cart

Davidson's Fixative (Hartmann's/ Davidson's Fixative)

This is rapid fixative. Results with good nuclear detail and minimal formalin pigment. Davidson's (Hartmann's) Fixative is useful for preparing tumors, bone marrow, gynecologic specimen, fatty breast, medical biopsies. Fixation of small specimen is rapid. Exposure to the fixative should be limited to 24 hours (tissue may then be transferred to neutral buffered formalin or 70% alcohol for storage).

Based on the volume of 1125 ml solution, there are 2 parts Formalin (35-39%), 3 parts Ethanol, 1 part Glacial Acetic acid, and 3 parts tap water.

RT	64133-10	Davidson's (Hartmann's) Fixative	1 L	82.00	Add to Cart
RT	64133-14	Davidson's (Hartmann's) Fixative	4 L	232.00	Add to Cart

Modified Davidson's Fixative

(14% Ethyl Alcohol, Denatured, 37.5% Formalin 37-39%, 6.25% Glacial Acetic Acid, and D.I. Water).

RT	64133-50	Modified Davidson's Fixative	1 L	39.00	Add to Cart
RT	64133-50-1G	Modified Davidson's Fixative	1 Gal.	150.00	Add to Cart

Dietrich's Fixative

Ethanol, formalin, glacial acetic acid, distilled water.

RT	15958-10	Dietrich's Fixative	10 x 10 ml	45.00	Add to Cart
RT	15958-20	Dietrich's Fixative	100 ml	44.00	Add to Cart
RT	15958-20-1L	Dietrich's Fixative	1 L	125.00	Add to Cart
RT	15958-20-1G	Dietrich's Fixative	1 Gal.	290.00	Add to Cart

Fixative/Decalcifier

• A solution that fixes and decalcifies in one step
• Prevents loss of cellular structure
• Enhances hematoxylin staining

RT	64148-01	Fixative/Decalcifier	1 L	52.00	Add to Cart
RT	64148-06	Fixative/Decalcifier	6 x 1 L	310.00	Add to Cart

Low Odor, 10% Formalin Solution; Histology Grade

A specially formulated odor-mask which neutralizes the formalin odor without affecting subsequent tissue processing, handling, or staining. Low odor, ready-to-use 10% formalin solution. May be used as a fixative or preservative of specimens. Its shelf life is 24 months.

RT	15678-01-1L	Low Odor, 10% Formalin; Histology Grade	1 L	30.00	Add to Cart
RT	15678-01	Low Odor, 10% Formalin; Histology Grade	4 L	95.00	Add to Cart
RT	15678-04	Low Odor, 10% Formalin; Histology Grade	4x4 L	364.00	Add to Cart

Buffered, Low-Odor 10% Formalin Solution

Low odor, prepared w/v, pH 6.9 to 7.1 at 25°C. 10% formalin in Phosphate buffer according to the A.F.I.P. Manual of Histology. This solution allows for long-term storage of tissue specimens.

RT	15741-01-1L	Low-Odor 10% Formalin Buffered	1 L	26.00	Add to Cart
RT	15741-01	Low-Odor 10% Formalin Buffered	4 L	65.00	Add to Cart
RT	15741-04	Low-Odor 10% Formalin Buffered	4x4 L	239.00	Add to Cart

Formalin, Neutral, Buffered 10% w/v in Acetate Buffer

Buffered with Sodium Acetate in accordance with the A.F.I.P. manual of Histology. Filtered and ready-to-use.
pH 6.0 to ph 7.1 at 20°C.

RT	15738-01	Formalin 10% Buffered in Acetate	1L	25.00	Add to Cart
RT	15738-04	Formalin 10% Buffered in Acetate	4L	57.00	Add to Cart
RT	15738-05	Formalin 10% Buffered in Acetate	5 Gal.	119.00	Add to Cart

Formalin, Neutral, Buffered 10% w/v in Phosphate Buffer

Buffered with phosphate in accordance with the A.F.I.P. Manual of Histology. Filtered and ready-to-use.
pH 6.9 to 7.1 at 25°C.

RT	15740	Formalin 10% Buffered in Phosphate	450 ml	19.50	Add to Cart
RT	15740-01	Formalin 10% Buffered in Phosphate	1 L	26.00	Add to Cart
RT	15740-04	Formalin 10% Buffered in Phosphate	4 L	65.00	Add to Cart
RT	15740-5G	Formalin 10% Buffered in Phosphate	5 Gal.	130.00	Add to Cart

Formalin 1% Non Buffered

RT	15734-01	Formalin 1% Non-Buffered	1 L	22.00	Add to Cart
RT	15734-04	Formalin 1% Non-Buffered	4 L	57.00	Add to Cart

Formalin 2%, Non-Buffered

RT	15735-01	Formalin 2% Non-Buffered	1 L	22.00	Add to Cart
RT	15735-04	Formalin 2% Non-Buffered	4 L	57.00	Add to Cart

Formalin 3%, Non-Buffered

RT	15735-10	Formalin 3% Non-Buffered	1 L	23.00	Add to Cart
RT	15735-20	Formalin 3% Non-Buffered	4 L	58.00	Add to Cart

Formalin 4%, Non-Buffered

RT	15735-30	Formalin 4% Non-Buffered	1 L	23.00	Add to Cart
RT	15735-40	Formalin 4% Non-Buffered	4 L	57.00	Add to Cart

Formalin 5%, Non-Buffered

RT	15735-50	Formalin 5% Non-Buffered	1 L	24.00	Add to Cart
RT	15735-60	Formalin 5% Non-Buffered	4 L	58.00	Add to Cart

TissuePrep Buffered 10% Formalin

Prefilled with 10% formalin Cat# 15740. Ready-to-use in pre-filled cups. Labeled in accordance to OSHA requirements, which gives you immediate information regarding handling and first aid procedures. Pre-measured in wide-mouth, leak-proof, opaque polypropylene containers which limits your exposure to formaldehyde and saves you time.

	Catalog #	Cup Size	Filled	Pack/cs	Price
RT	15742-10	15 ml	10 ml	50	77.00	Add to Cart
RT	15742-20	30 ml	20 ml	50	98.00	Add to Cart
RT	15742-30	60 ml	30 ml	20	44.00	Add to Cart
RT	15742-60	120 ml	60 ml	20	48.00	Add to Cart

Paraformaldehyde, Prepared Fixatives

In addition to our complete line of Formaldehyde solutions we have now added to the line.

Paraformaldehyde 0.5% in 0.1M phosphate buffer pH7.4

RT	15951-10	Paraformaldehyde, 0.5% each in 0.1M Phosphate Buffer	10 x 10 ml	39.00	Add to Cart
RT	15951-15	Paraformaldehyde, 0.5% each in 0.1M Phosphate Buffer	500 ml	105.00	Add to Cart
RT	15951-15-1L	Paraformaldehyde, 0.5% each in 0.1M Phosphate Buffer	1 L	160.00	Add to Cart
RT	15951-15-1G	Paraformaldehyde, 0.5% each in 0.1M Phosphate Buffer	1 Gal.	395.00	Add to Cart

Paraformaldehyde 1% Aqueous

RT	15953-10	Paraformaldehyde, 1% each Aqueous	10 x 10 ml	27.00	Add to Cart
RT	15953-15	Paraformaldehyde, 1% each Aqueous	500 ml	97.00	Add to Cart
RT	15953-15-1L	Paraformaldehyde, 1% each Aqueous	1 L	150.00	Add to Cart
RT	15953-15-1G	Paraformaldehyde, 1% each Aqueous	1 Gal.	360.00	Add to Cart

Paraformaldehyde 1% in 0.1M Phosphate Buffer pH, 7.4

RT	15952-10	Paraformaldehyde, 1% each in 0.1M Phosphate Buffer	10 x 10 ml	41.00	Add to Cart
RT	15952-15	Paraformaldehyde, 1% each in 0.1M Phosphate Buffer	500 ml	105.00	Add to Cart
RT	15952-15-1L	Paraformaldehyde, 1% each in 0.1M Phosphate Buffer	1 L	160.00	Add to Cart
RT	15952-15-1G	Paraformaldehyde, 1% each in 0.1M Phosphate Buffer	1 Gal.	395.00	Add to Cart

Paraformaldehyde 1% Glut 2% in 0.1M Phosphate buffer, pH 7.4

RT	15949-30	Paraformaldehyde 1% Glutaraldehyde 2% in 0.1M Phosphate Buffer	10 x 10 ml	41.00	Add to Cart
RT	15949-35	Paraformaldehyde 1% Glutaraldehyde 2% in 0.1M Phosphate Buffer	500 ml	108.00	Add to Cart
RT	15949-35-1L	Paraformaldehyde 1% Glutaraldehyde 2% in 0.1M Phosphate Buffer	1 L	162.00	Add to Cart
RT	15949-35-1G	Paraformaldehyde 1% Glutaraldehyde 2% in 0.1M Phosphate Buffer	1 Gal.	398.00	Add to Cart

Paraformaldehyde 3% Glutaraldehyde 0.35% in 0.1M Sodium Cacodylate, pH 7.4

RT	15949-50	Paraformaldehyde 3% Glutaraldehyde 0.35% in 0.1M Sodium Cacodylate Buffer	10 x 10 ml	44.00	Add to Cart
RT	15949-60	Paraformaldehyde 3% Glutaraldehyde 0.35% in 0.1M Sodium Cacodylate Buffer	500 ml	115.00	Add to Cart
RT	15949-60-1L	Paraformaldehyde 3% Glutaraldehyde 0.35% in 0.1M Sodium Cacodylate Buffer	1 L	185.00	Add to Cart
RT	15949-60-1G	Paraformaldehyde 3% Glutaraldehyde 0.35% in 0.1M Sodium Cacodylate Buffer	1 Gal.	398.00	Add to Cart

Paraformaldehyde 4% and Glutaraldehyde 1% in Phosphate Buffer, pH 7.0

RT	15949-70	Paraformaldehyde 4% Glutaraldehyde 1% in 0.1M Phosphate Buffer	10 x 10 ml	48.00	Add to Cart
RT	15949-80	Paraformaldehyde 4% Glutaraldehyde 1% in 0.1M Phosphate Buffer	500 ml	115.00	Add to Cart
RT	15949-80-1L	Paraformaldehyde 4% Glutaraldehyde 1% in 0.1M Phosphate Buffer	1 L	185.00	Add to Cart
RT	15949-80-1G	Paraformaldehyde 4% Glutaraldehyde 1% in 0.1M Phosphate Buffer	1 Gal.	398.00	Add to Cart

Paraformaldehyde 4% in 0.1M Sodium Cacodylate, pH7.4

RT	15952-10S	Paraformaldehyde 4% in 0.1M Sodium Cacodylate Buffer	10 x 10 ml	51.00	Add to Cart
RT	15952-15S	Paraformaldehyde 4% in 0.1M Sodium Cacodylate Buffer	500 ml	131.00	Add to Cart
RT	15952-15S-1L	Paraformaldehyde 4% in 0.1M Sodium Cacodylate Buffer	1 L	142.00	Add to Cart
RT	15952-15S-1G	Paraformaldehyde 4% in 0.1M Sodium Cacodylate Buffer	1 Gal.	405.00	Add to Cart

Paraformaldehyde 4% in 0.1M Phosphate Buffer, pH 7.4

RT	15735-10S	Paraformaldehyde 4% in 0.1M Phosphate Buffer	10 x 10 ml	39.00	Add to Cart
RT	15735-20S	Paraformaldehyde 4% in 0.1M Phosphate Buffer	500 ml	109.00	Add to Cart
RT	15735-20S-1L	Paraformaldehyde 4% in 0.1M Phosphate Buffer	1 L	175.00	Add to Cart
RT	15735-20S-1G	Paraformaldehyde 4% in 0.1M Phosphate Buffer	1 Gal.	430.00	Add to Cart

Paraformaldehyde 4% in 0.2M Phosphate Buffer, pH 7.4

RT	15735-30S	Paraformaldehyde 4% in 0.2M Phosphate Buffer	10 x 10 ml	38.00	Add to Cart
RT	15735-40S	Paraformaldehyde 4% in 0.2M Phosphate Buffer	500 ml	108.00	Add to Cart
RT	15735-40S-1L	Paraformaldehyde 4% in 0.2M Phosphate Buffer	1 L	175.00	Add to Cart
RT	15735-40S-1G	Paraformaldehyde 4% in 0.2M Phosphate Buffer	1 Gal.	430.00	Add to Cart

Paraformaldehyde 4% in 0.1M Phosphate Buffer, pH 7.2

RT	15735-50S	Paraformaldehyde 4% in 0.1M Phosphate Buffer	10 x 10 ml	44.00	Add to Cart
RT	15735-60S	Paraformaldehyde 4% in 0.1M Phosphate Buffer	500 ml	112.00	Add to Cart
RT	15735-60S-1L	Paraformaldehyde 4% in 0.1M Phosphate Buffer	1 L	175.00	Add to Cart
RT	15735-60S-1G	Paraformaldehyde 4% in 0.1M Phosphate Buffer	1 Gal.	430.00	Add to Cart

Paraformaldehyde 4% in 0.2M Phosphate Buffer, pH 7.2

RT	15735-70	Paraformaldehyde 4% in 0.2M Phosphate Buffer	10 x 10 ml	44.00	Add to Cart
RT	15735-80	Paraformaldehyde 4% in 0.2M Phosphate Buffer	500 ml	110.00	Add to Cart
RT	15735-80-1L	Paraformaldehyde 4% in 0.2M Phosphate Buffer	1 L	175.00	Add to Cart
RT	15735-80-1G	Paraformaldehyde 4% in 0.2M Phosphate Buffer	1 Gal.	430.00	Add to Cart

Paraformaldehyde 4% in 0.1M Phosphate BufferSaline, pH7.4

RT	15735-85	Paraformaldehyde 4% in 0.1M Phosphate Buffer Saline	10 x 10 ml	45.00	Add to Cart
RT	15735-90	Paraformaldehyde 4% in 0.1M Phosphate Buffer Saline	500 ml	114.00	Add to Cart
RT	15735-90-1L	Paraformaldehyde 4% in 0.1M Phosphate Buffer Saline	1 L	176.00	Add to Cart
RT	15735-90-1G	Paraformaldehyde 4% in 0.1M Phosphate Buffer Saline	1 Gal.	432.00	Add to Cart

Paraformaldehyde 4% in 0.2M Phosphate Buffer Saline, pH 7.4

RT	15735-95	Paraformaldehyde 4% in 0.2M Phosphate Buffer	10 x 10 ml	46.00	Add to Cart
RT	15735-100	Paraformaldehyde 4% in 0.2M Phosphate Buffer	500 ml	115.00	Add to Cart
RT	15735-100-1L	Paraformaldehyde 4% in 0.2M Phosphate Buffer	1 L	176.00	Add to Cart
RT	15735-100-1G	Paraformaldehyde 4% in 0.2M Phosphate Buffer	1 Gal.	432.00	Add to Cart

Formaldehyde, Reagent, A.C.S. (Formalin)

HCHO F.W. 30.03 CAS #50-00-0
Specific Gravity: 1.09
A.C.S.

Specifications:

Assay		35.5 to 38.0%
Residue after ignition		0.005%
Color (A.P.H.A.)		10
Sulfate (SO4)		0.002%
Heavy Metal (as Pb)		5ppm
Titrate Acid		0.006 meq/g
Chloride (Cl)		5ppm
Iron (Fe)		5ppm

RT	15680	Formaldehyde, Reagent, A.C.S.	450 ml	16.00	Add to Cart
RT	15681	Formaldehyde, Reagent, A.C.S.	4 x 450 ml	54.00	Add to Cart
RT	15682	Formaldehyde, Reagent, A.C.S.	1 qt (946 ml)	25.00	Add to Cart
RT	15682-1G	Formaldehyde, Reagent, A.C.S.	1 Gal.	79.00	Add to Cart
RT	15682-5G	Formaldehyde, Reagent, A.C.S.	5 Gal.	192.00	Add to Cart

Formaldehyde, 37%, Microfiltered (Formalin Microfiltered)

HCHO F.W. 30.03 CAS# 50-00-0

Microfiltered with a 0.45 micron membrane; suitable as an EM fixative when a methanol free solution is not necessary.

RT

15686

10 x 10 ml

34.00

Add to Cart

Formaldehyde

(Paraformaldehyde) GLUTARALDEHYDE

Karnovsky's Fixative Kit, please click here.

RT	15720		kit	28.00	Add to Cart
RT	15730		Lot of 5 kits	126.00	Add to Cart

Fixative Reagent Grade (Karnovsky's)

This fixative contains 3% glutaraldehyde and 2% formaldehyde in 0.1M phosphate buffer, pH 7.4.

Karnovsky's with formalin 2% and Biological glutaraldehyde 3% in 0.1m Phosphate Buffer.

RT	15731-10	Karnovsky's Fixative Reagent Grade	1 L	260.00	Add to Cart
RT	15731-10-1G	Karnovsky's Fixative Reagent Grade	1 Gal.	825.00	Add to Cart
RT	15731-10-5G	Karnovsky's Fixative Reagent Grade	5 Gal.	2000.00	Add to Cart

Karnovsky's with paraformaldehyde solution and EM grade glutaraldehyde

Please Note: This kit can be formulated to fit your requirements. Please contact us.

0-5°C	15732-10	Karnovsky's Fixative EM Grade	1 L	286.00	Add to Cart
0-5°C	15732-10-1G	Karnovsky's Fixative EM Grade	1 Gal.	850.00	Add to Cart
0-5°C	15732-10-5G	Karnovsky's Fixative EM Grade	5 Gal.	2200.00	Add to Cart

Formaldehyde Aqueous Solution (Paraformaldehyde Aqueous Solution) EM Grade

Paraformaldehyde – methanol free solution. A more efficient and rapid penetrant fixative used in combination with Glutaraldehyde and Acrolein fixatives. Easy to break, prescored, 10 ml ampoules sealed under inert gas, or 100 ml bottles.

CONTAINS NO PRESERVATIVES

RT	15700	16% Aqueous Solution		10 ml	6.00	Add to Cart
RT	15710	16% Aqueous Solution		10x10 ml	31.00	Add to Cart
RT	15710-S	16% Aqueous Solution		100 ml bottle	30.00	Add to Cart
RT	15711	16% Aqueous Solution		10 bxs/lot	280.00	Add to Cart
RT	15700-1L	16% Aqueous Solution		1 L	260.00	Add to Cart
RT	15700-1G	16% Aqueous Solution		1 Gal.	775.00	Add to Cart
RT	15700-5G	16% Aqueous Solution		5 Gal.	3100.00	Add to Cart
RT	15712	10% Aqueous Solution		10x10 ml	30.00	Add to Cart
RT	15712-S	10% Aqueous Solution		100 ml	29.00	Add to Cart
RT	15712-1L	10% Aqueous Solution		1 L	240.00	Add to Cart
RT	15712-1G	10% Aqueous Solution		1 Gal.	750.00	Add to Cart
RT	15712-5G	10% Aqueous Solution		5 Gal.	2950.00	Add to Cart
RT	15713	20% Aqueous Solution		10x10 ml	34.00	Add to Cart
RT	15713-S	20% Aqueous Solution		100 ml	33.00	Add to Cart
RT	15713-1L	20% Aqueous Solution		1 L	295.00	Add to Cart
RT	15713-1G	20% Aqueous Solution		1 Gal.	990.00	Add to Cart
RT	15713-5G	20% Aqueous Solution		5 Gal.	4150.00	Add to Cart
RT	15714	32% Aqueous Solution		10x10 ml	39.00	Add to Cart
RT	15714-S	32% Aqueous Solution		100 ml	38.00	Add to Cart
RT	15714-1L	32% Aqueous Solution		1 L	299.00	Add to Cart
RT	15714-1G	32% Aqueous Solution		1 Gal.	995.00	Add to Cart
RT	15714-5G	32% Aqueous Solution		5 Gal.	4200.00	Add to Cart

In addition to our complete line of Formaldehyde solutions shown above we have now added to the line.

Since the DOT (Department of Transportation) has changed the definition of what a non dangerous solution of formaldehyde is to under 10%, we are now offering an 8% and a 4% solution. This will allow us to ship overnight as well as overseas without any hazardous charges saving our customers a good deal of money.

The quality of our 8% and 4% aqueous Paraformaldehyde solution is as good as all of the other concentrations that we offer.

CONTAINS NO PRESERVATIVES

RT	157-4	4% Aqueous Solution		10x10 ml	27.00	Add to Cart
RT	157-4-100	4% Aqueous Solution		100 ml	26.00	Add to Cart
RT	157-4-1L	4% Aqueous Solution		1 L	225.00	Add to Cart
RT	157-4-1G	4% Aqueous Solution		1 Gal.	720.00	Add to Cart
RT	157-4-5G	4% Aqueous Solution		5 Gal.	2950.00	Add to Cart
RT	157-8	8% Aqueous Solution		10x10 ml	26.00	Add to Cart
RT	157-8-100	8% Aqueous Solution		100 ml	25.00	Add to Cart
RT	157-8-1L	8% Aqueous Solution		1 L	220.00	Add to Cart
RT	157-8-1G	8% Aqueous Solution		1 Gal.	700.00	Add to Cart
RT	157-8-5G	8% Aqueous Solution		5 Gal.	2850.00	Add to Cart

Formaldehyde/Zinc Fixative

Prepared: Ready-to-use.

Features

• Preserves fine nuclear details
• Good for immunostaining
• Contains no mercury
• Can be used with automated tissue processors
• Works well with standard and special stains because zinc is a stain enhancing mordant

RT	15675-01	Formaldehyde/Zinc Fixative	1 L	21.00	Add to Cart
RT	15675-04	Formaldehyde/Zinc Fixative	4 L	69.00	Add to Cart

Glyoxal 3% Fixative, Ready to Use

An alternative fixative to Formalin and Formaldehyde

EMS is proud to introduce an alternative to Formalin and Formaldehyde in many different applications.

Our unique kit comes premixed and measured and ready to use. The kit consists of 2 vials: Solution A & Solution B (Absolute Ethanol, Glyoxal Solution, Acetic Acid and water).

Instructions

Add solution A to solution B. Vortex the two solutions together and bring to a pH of 4.5 with the addition of NaOH 1M. The solution needs to be used immediately once mixed.

References

Sabatini DD. et al (1963) "The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation". J. Cell Biology. 17:19-58

Janigan DT. et al (1965) "The effects of aldehyde fixation on acid phosphatase activity in tissue blocks". J. Histochemistry. Cytochemistry. 13: 476-483

Dapson RW. et al (2006) "Glyoxal fixation and its relationship to immunocytochemistry." J. Histotechnology. 29: 65-76

Dapson RW. Et al (2007) "Glyoxal fixation: how it works and why it only occasionally needs antigen retrieval" lnforma healthcare, Dapson & Dapson, LLC

Richter K. et al (2018) "Glyoxal as an alternative to formaldehyde in immunostaining and super-resolution microscopy" The EMBO Journal. 37: 139-159

2-8°C

16525

Glyoxal 3% Fixative

100 ml

52.00

Add to Cart

Ampoules Breakers and Crackers

Please see the Sample Preparation section.

Perfusion Wash and Fixative Ready to Use

For all of the best results we offer ready to use Perfusion washes and fixatives which are recommended by the NeuroScience Association. All of our solutions are ready made and are of the highest quality.

We have three groups of solutions commercially available.

Group I:

Perfusion wash and Fixative Technical Grade. To be used for Standard stains:

Perfusion Wash (1220SK): Phosphate Buffered Saline 01.M Phosphate Buffer pH 7.2-7.4

Perfusion Fixative (1221SK): 10% Phosphate Buffered Formaldehyde

4-8°C	1220SK	Perfusion Wash, Technical		1000 ml	34.00	Add to Cart
4-8°C	1220SK-1G	Perfusion Wash, Technical		1 Gal.	105.00	Add to Cart
4-8°C	1220SK-5G	Perfusion Wash, Technical		5 Gal.	450.00	Add to Cart
4-8°C	1221SK	Perfusion Fixative, Technical		1000 ml	48.00	Add to Cart
4-8°C	1221SK-1G	Perfusion Fixative, Technical		1 Gal.	150.00	Add to Cart
4-8°C	1221SK-5G	Perfusion Fixative, Technical		5 Gal.	625.00	Add to Cart

Group II:

Perfusion wash and Fixative Reagent Grade. To be used for Standard and ImmunoHistochemistry Stains.

Perfusion Wash (1219SK): Phosphate Buffered Saline 0.1M Phosphate Buffer pH 7.2-7.4

Perfusion Fixative (1224SK): Paraformaldehyde 4%/Sucrose 4% in Phosphate Buffer 0.1M Ph 7.2

4-8°C	1219SK	Perfusion Wash, Reagent		1000 ml	32.00	Add to Cart
4-8°C	1219SK-1G	Perfusion Wash, Reagent		1 Gal.	102.00	Add to Cart
4-8°C	1219SK-5G	Perfusion Wash, Reagent		5 Gal.	445.00	Add to Cart
4-8°C	1224SK	Perfusion Fixative, Reagent		1000 ml	49.00	Add to Cart
4-8°C	1224SK-1G	Perfusion Fixative, Reagent		1 Gal.	156.00	Add to Cart
4-8°C	1224SK-5G	Perfusion Fixative, Reagent		5 Gal.	700.00	Add to Cart

Group III:

Perfusion wash and Fixative Superior Reagent Grade.

For the best results use this group. This is Sodium cacodylate based for NSA'S Disintegrative Degeneration Stain.

Perfusion Wash (1222SK): 0.8% NaCl/0.4% Dextrose/0.8% Sucrose/ 0.023% CaCl2 /Sodium.

Cacodylate 0.034%

Perfusion Fixative (1223SK): 4% Paraformaldehyde/4% Sucrose/ 1.4% Sodium Cacodylate.

4-8°C	1222SK	Perfusion Wash, Super Reagent		1000 ml	35.00	Add to Cart
4-8°C	1222SK-1G	Perfusion Wash, Super Reagent		1 Gal.	106.00	Add to Cart
4-8°C	1222SK-5G	Perfusion Wash, Super Reagent		5 Gal.	450.00	Add to Cart
4-8°C	1223SK	Perfusion Fixative, Super Reagent		1000 ml	52.00	Add to Cart
4-8°C	1223SK-1G	Perfusion Fixative, Super Reagent		1 Gal.	165.00	Add to Cart
4-8°C	1223SK-5G	Perfusion Fixative, Super Reagent		5 Gal.	725.00	Add to Cart

Technical Tip:

The Fixative for Use in Fixing Cornea

CoreDish™ Multiple Biopsy Containers Half Prefilled with 10% Formalin

Few recommendations concerning how the biopsies should be handled have been published. Performing a large number of biopsies means an increase in the number of containers handled and consequently a technical overload of the transmission network, which occurs without any financial counterpart. A new approach had to be developed in order to increase productivity.

We are proud to offer a multi-compartment container in the shape of a dish and half prefilled with 10% Neutral buffered Formalin, for holding and transporting biopsies. It is supplied with a leak-proof closure with O-ring ensuring total protection of contents. It conforms to OSHA directives. These CoreDisn™ measure only 15mm H x 95 mm Diameter. Each compartment is clearly identified to allow proper placement and visualization of the biopsy being inserted. Thanks to CoreDish™ it is no more necessary to use a multiple of individual containers, thereby reducing cost and risks of confusion.

CoreDish™ available in many configurations to serve in different biopsy, such as prostate, upper GI tract and lower GI tract. A label allows recording the related information, patient ID, doctor, date and time.

M970-D5B-2 CoreDish™ Breast Biopsy Container

Half Prefilled with 10% Formalin

This multi-compartment container (out of five compartments, four are labeled: LUQ, RUQ, LLQ, RLQ) in the shape of a dish and half prefilled with 10% Neutral Buffered Formalin, for holding and transporting biopsies. It is supplied with a leak-proof screw closure with O-ring ensuring total protection of contents. It conforms to OSHA directives. The dish measures only 15 x 95 mm in diameter. Each compartment is clearly identified to allow proper placement and visualization of the breast biopsy being inserted. A writing area for patient information is provided.

RT

15736-10

Breast, 5 compartments

10/pk

64.00

Add to Cart

M970-D8P CoreDish™ Prostate Biopsy Container

Half Prefilled with 10% Formalin

For prostate biopsies. Eight compratments. Leakproof seal with O-ring lid. An area for patient information is provided. Seven labeled compartments: Base, Lateral Base, Medium, Lateral Medium, Apex, Lateral Apex.

RT

15736-20

Prostate, 8 Compartments

10/pk

64.00

Add to Cart

M970-D8UGI CoreDish™ Upper GI Biopsy Container

Half Prefilled with 10% Formalin

For upper GI track biopsies. Eight compratments. Leakproof seal with O-ring lid. An area for patient information is provided. Seven labeled compartments: Gastric Card, Gastric Body, GE Junction, Gastric ATR, Distal Esophage, Pylorus, Duodeum.

RT

15736-30

Upper GI, 8 Compartments

10/pk

64.00

Add to Cart

M970-D8LGI CoreDish™ Lower Gi Biopsy Container

Half Prefilled with 10% Formalin

For lower GI track biopsies. Twelve compratments. Leakproof seal with O-ring lid. An area for patient information is provided. Ten labeled compartments: Proximal Flexure Colon, Hepatic Fleure Colon, Distal Transverse Colon, Ascending Colon, Splenic flexure Colon, Cecum, Decending Colon, Terminal lleum, Rectum, Sigmmoid Colon.

RT

15736-40

Lower GI, 12 Compartments

10/pk

64.00

Add to Cart

M970-D12P CoreDish™ Prostate Biopsy Container

Half Prefilled with 10% Formalin

For prostate biopsies. Twelve compratments. Leakproof seal with O-ring lid. An area for patient information is provided. Twelve labeled compartments: L Base, R Base, L Lateral Base, R Lateral Base, L Medium, R Medium, L Lateral Medium, R Lateral Medium, L Lateral, L Apex, R Apex, R Lateral Apex.

RT

15736-50

Prostate, 12 Compartments

10/pk

64.00

Add to Cart

Glutaraldehyde

OCHCH₂CH₂CH₂CHO F.W. 100.12 CAS #111-30-8
Specific Gravity @20/20°C: 1.131

EM Grade Distillation Purified

• The Universal Fixative for Electron Microscopy
• Purified to EM Grade from commercial grade by "all-glass vacuum-distillation."
• THE HIGHEST PURITY, free from polymers and other contaminants
• Each lot is tested and assayed in our lab before we ship and guarantee its purity
• Sealed under dry nitrogen
• Refrigerated prior to shipment

After glutaraldehyde has been purified to EM grade, it has a high risk of polymerizing if it is not handled properly. Electron Microscopy Sciences takes special precautions to assure a polymer-free, pure glutaraldehyde.

Every lot of our EM grade aqueous glutaraldehyde is assayed prior to shipping assuring you consistent purity and the highest possible quality. If stored properly, our Glutaraldehyde has a very long shelf life.

We package our aqueous glutaraldehyde in 10 ml, single dose ampoules, sealed under dry nitrogen, and kept refrigerated until it is shipped. We offer our glutaraldehyde in a wide range of percentages to simplify mixing procedures and avoiding waste.

EM grade aqueous glutaraldehyde, stored in volumes greater than 100 ml, with repeated defrosting and recapping, will polymerize in time, producing an absorption peak of 235 nm rather than the 280 nm of pure EM grade. The presence of polymerized glutaraldehyde greatly reduces its efficiency as a cross-linking agent, preventing proper fixation. For this reason, we provide 10 ml ampoules and 100 ml bottle quantities for larger applications.

8% Aqueous Solution

4 to (-18)°C	16000		10 ml ampoule	6.00	Add to Cart
4 to (-18)°C	16019		Box 10x2ml	23.00	Add to Cart
4 to (-18)°C	16020		Box 10 x 10 ml ampoules	27.00	Add to Cart
4 to (-18)°C	16021	same as 16020	10 bxs	260.00	Add to Cart
4 to (-18)°C	16022	same as 16020	20 bxs	445.00	Add to Cart
4 to (-18)°C	16010		100 ml bottle	26.00	Add to Cart
4 to (-18)°C	16010-1L		1 L	225.00	Add to Cart

10% Aqueous Solution

4 to (-18)°C	16100		10 ml ampoule	6.00	Add to Cart
4 to (-18)°C	16120		Box 10 x 10 ml ampoules	30.00	Add to Cart
4 to (-18)°C	16121	same as 16120	10 bxs	278.00	Add to Cart
4 to (-18)°C	16122	same as 16120	20 bxs	475.00	Add to Cart
4 to (-18)°C	16110		100 ml bottle	26.00	Add to Cart
4 to (-18)°C	16110-1L		1 L	225.00	Add to Cart

25% Aqueous Solution

4 to (-18)°C	16200		10 ml ampoule	7.00	Add to Cart
4 to (-18)°C	16220		Box 10 x 10 ml ampoules	33.00	Add to Cart
4 to (-18)°C	16221	same as 16220	10 bxs	315.00	Add to Cart
4 to (-18)°C	16222	same as 16220	20 bxs	550.00	Add to Cart
4 to (-18)°C	16210		100 ml bottle	26.00	Add to Cart
4 to (-18)°C	16210-1L		1 L	230.00	Add to Cart

50% Aqueous Solution

4 to (-18)°C	16300		10 ml ampoule	7.50	Add to Cart
4 to (-18)°C	16320		Box 10 x 10 ml ampoules	45.00	Add to Cart
4 to (-18)°C	16321	same as 16320	10 bxs	430.00	Add to Cart
4 to (-18)°C	16322	same as 16320	20 bxs	780.00	Add to Cart
4 to (-18)°C	16310		100 ml bottle	44.00	Add to Cart
4 to (-18)°C	16310-1L		1 L	350.00	Add to Cart

70% Aqueous Solution

4 to (-18)°C	16350		10 ml ampoule	8.50	Add to Cart
4 to (-18)°C	16360		box 10 x 10 ml ampoules	62.00	Add to Cart
4 to (-18)°C	16365		box 10 x 2ml ampoules	37.00	Add to Cart

Biological Grade

4 to (-18)°C	16400	25% Solution	450 ml	20.00	Add to Cart
4 to (-18)°C	16401	25% Solution	4x450 ml	76.00	Add to Cart
4 to (-18)°C	16410	25% Solution	1 gal.	74.00	Add to Cart
4 to (-18)°C	16411	25% Solution	4x1 gal.	269.00	Add to Cart
4 to (-18)°C	16500	50%Solution	450 ml	23.00	Add to Cart
4 to (-18)°C	16501	50% Solution	4x450 ml	80.00	Add to Cart
4 to (-18)°C	16520	50% Solution	1 liter	43.00	Add to Cart
4 to (-18)°C	16521	50% Solution	4x1 liter	154.00	Add to Cart

Serum Vial Aqueous Glutaraldehyde

Over the years, many of our valued customers have requested purchasing our aqueous Glutaraldehyde in serum vials. This packaging allows the user to puncture the special non-reactive seal with a hypodermic syringe and draw out the exact quantity of aqueous Glutaraldehyde needed without opening the container. This packaging assures freshness and purity of the aqueous Glutaraldehyde at all times.

4 to (-18)°C	16216	25% EM Grade Aqueous Glutaraldehyde in Serum Vial	10 x10 ml	46.00	Add to Cart
4 to (-18)°C	16214	25% EM Grade Aqueous Glutaraldehyde in Serum Vial	100 ml	38.00	Add to Cart
4 to (-18)°C	16316	50% EM Grade Aqueous Glutaraldehyde in Serum Vial	10 x10 ml	58.00	Add to Cart
4 to (-18)°C	16314	50% EM Grade Aqueous Glutaraldehyde in Serum Vial	100 ml	47.00	Add to Cart

* All other concentrations are available in serum vials upon request.

For any special concentration, or packaging requirements other than those already listed, please call us today. We will manufacture in accordance to your specifications.

Anhydrous EM Grade Glutaraldehyde: Specially Prepared-10% Solution

Rapid-Freezing on a polished metal surface, at the temp. of liquid helium or liquid nitrogen, followed by freeze-substitution in osmium-acetone, has been shown to be a very useful technique for capturing rapid cellular events and for preserving cell ultrastructure.
* John M. Robinson and Morris J. Karnovsky, 1991. "Rapid-freezing Cytochemistry: Preservation of Tubular Lysosomes and Enzyme Activity". J. of Histochem., Cytochem. Vol. 39, No. 6, pp 787-792.

Ordering Information: (please call for additional information)

0-5°C	16530	10% in Acetone		10 x 10 ml	88.00	Add to Cart
0-5°C	16531	10% in Ethanol		10 x 10 ml	88.00	Add to Cart
0-5°C	16532	10% in Methanol		10 x 10 ml	88.00	Add to Cart
0-5°C	16533	10% in Solvent of your choice		10 x 10 ml	88.00	Add to Cart

We would be pleased to fill your specific glutaraldehyde needs. Please call for prices on special concentrations or size requirements.

Glutaraldehyde, Pre-Made, Ready-to-Use, in Buffer

Glutaraldehyde 0.625% in 0.1M Phosphate Buffer, pH 7.4

0-5°C	16535-05	Glutaraldehyde 0.625% in Phosphate Buffer, pH 7.4	500 ml	145.00	Add to Cart
0-5°C	16535-10	Glutaraldehyde 0.625% in Phosphate Buffer, pH 7.4	1 L	272.00	Add to Cart
0-5°C	16535-10-1G	Glutaraldehyde 0.625% in Phosphate Buffer, pH 7.4	1 Gal.	875.00	Add to Cart

Glutaraldehyde 2% in 0.1M Phosphate Buffer, pH 7.4

0-5°C	16536-05	Glutaraldehyde 2% in Phosphate Buffer, pH 7.4	500 ml	145.00	Add to Cart
0-5°C	16536-10	Glutaraldehyde 2% in Phosphate Buffer, pH 7.4	1 L	280.00	Add to Cart
0-5°C	16536-10-1G	Glutaraldehyde 2% in Phosphate Buffer, pH 7.4	1 Gal.	880.00	Add to Cart

Glutaraldehyde 2% in 0.1M Sodium Cacodylate Buffer, pH 7.4

0-5°C	16536-15	Glutaraldehyde 2% in Sodium Cacodylate Buffer, pH 7.4	500 ml	155.00	Add to Cart
0-5°C	16536-20	Glutaraldehyde 2% in Sodium Cacodylate Buffer, pH 7.4	1 L	292.00	Add to Cart
0-5°C	16536-20-1G	Glutaraldehyde 2% in Sodium Cacodylate Buffer, pH 7.4	1 Gal.	925.00	Add to Cart

Glutaraldehyde 2.5% in 0.1M Phosphate Buffer, pH 7.4

0-5°C	16537-05	Glutaraldehyde 2.5% in Phosphate Buffer, pH 7.4	500 ml	162.00	Add to Cart
0-5°C	16537-10	Glutaraldehyde 2.5% in Phosphate Buffer, pH 7.4	1 L	295.00	Add to Cart
0-5°C	16537-10-1G	Glutaraldehyde 2.5% in Phosphate Buffer, pH 7.4	1 Gal.	940.00	Add to Cart

Glutaraldehyde 2.5% in 0.1M Sodium Cacodylate Buffer, pH 7.4

0-5°C	16537-15	Glutaraldehyde 2.5% in Sodium Cacodylate Buffer, pH 7.4	500 ml	161.00	Add to Cart
0-5°C	16537-20	Glutaraldehyde 2.5% in Sodium Cacodylate Buffer, pH 7.4	1 L	300.00	Add to Cart
0-5°C	16537-20-1G	Glutaraldehyde 2.5% in Sodium Cacodylate Buffer, pH 7.4	1 Gal.	1000.00	Add to Cart

Glutaraldehyde 3% in 0.1M Phosphate Buffer, pH 7.4

0-5°C	16538-05	Glutaraldehyde 3% in Phosphate Buffer, pH 7.4	500 ml	166.00	Add to Cart
0-5°C	16538-10	Glutaraldehyde 3% in Phosphate Buffer, pH 7.4	1 L	300.00	Add to Cart
0-5°C	16538-10-1G	Glutaraldehyde 3% in Phosphate Buffer, pH 7.4	1 Gal.	1000.00	Add to Cart

Glutaraldehyde 3% in 0.1M Sodium Cacodylate Buffer, pH 7.4

0-5°C	16538-15	Glutaraldehyde 3% in Sodium Cacodylate Buffer, pH 7.4	500 ml	168.00	Add to Cart
0-5°C	16538-20	Glutaraldehyde 3% in Sodium Cacodylate Buffer, pH 7.4	1 L	305.00	Add to Cart
0-5°C	16535-20-1G	Glutaraldehyde 3% in Sodium Cacodylate Buffer, pH 7.4	1 Gal.	1010.00	Add to Cart

Glutaraldehyde 3% Sodium Phosphate Buffer, pH 7.2

0-5°C	16539-45	Glutaraldehyde 3% in Sodium Phosphate Buffer, pH 7.2	10x10 ml	39.00	Add to Cart
0-5°C	16539-50	Glutaraldehyde 3% in Sodium Phosphate Buffer, pH 7.2	500 ml	139.00	Add to Cart
0-5°C	16539-55	Glutaraldehyde 3% in Sodium Phosphate Buffer, pH 7.2	1 L	282.00	Add to Cart
0-5°C	16539-55-1G	Glutaraldehyde 3% in Sodium Phosphate Buffer, pH 7.2	1 Gal.	975.00	Add to Cart

Glutaraldehyde 4% in 0.1m Phosphate Buffer, pH 7.3

0-5°C	16539-60	Glutaraldehyde 4% in 0.1m Phosphate Buffer, pH 7.3	10x10 ml	41.00	Add to Cart
0-5°C	16539-65	Glutaraldehyde 4% in 0.1m Phosphate Buffer, pH 7.3	500 ml	139.00	Add to Cart
0-5°C	16539-70	Glutaraldehyde 4% in 0.1m Phosphate Buffer, pH 7.3	1 L	278.00	Add to Cart
0-5°C	16539-70-1G	Glutaraldehyde 4% in 0.1m Phosphate Buffer, pH 7.3	1 Gal.	850.00	Add to Cart

Glutaraldehyde 2% Paraformaldehyde 2% in Phosphate Buffer, pH 7.4

0-5°C	16536-06	Glutaraldehyde 2% Paraformaldehyde 2% in Phosphate Buffer, pH 7.4	100 ml	40.00	Add to Cart
0-5°C	16536-07	Glutaraldehyde 2% Paraformaldehyde 2% in Phosphate Buffer, pH 7.4	500 ml	159.00	Add to Cart
0-5°C	16536-08	Glutaraldehyde 2% Paraformaldehyde 2% in Phosphate Buffer, pH 7.4	1 L	286.00	Add to Cart
0-5°C	16536-08-1G	Glutaraldehyde 2% Paraformaldehyde 2% in Phosphate Buffer, pH 7.4	1 Gal.	915.00	Add to Cart

Glutaraldehyde 2.5% Paraformaldehyde 1% in Phosphate Buffer, pH 7.4

0-5°C	16537-06	Glutaraldehyde 2.5% Paraformaldehyde 1% in Phosphate Buffer, pH 7.4	100 ml	38.00	Add to Cart
0-5°C	16537-07	Glutaraldehyde 2.5% Paraformaldehyde 1% in Phosphate Buffer, pH 7.4	500 ml	158.00	Add to Cart
0-5°C	16537-08	Glutaraldehyde 2.5% Paraformaldehyde 1% in Phosphate Buffer, pH 7.4	1 L	284.00	Add to Cart
0-5°C	16537-08-1G	Glutaraldehyde 2.5% Paraformaldehyde 1% in Phosphate Buffer, pH 7.4	1 Gal.	914.00	Add to Cart

Glutaraldehyde 2.5% Aqueous

0-5°C	16537-16	Glutaraldehyde 2.5% Aqueous	10x10 ml	26.00	Add to Cart
0-5°C	16537-17	Glutaraldehyde 2.5% Aqueous	500 ml	68.00	Add to Cart
0-5°C	16537-18	Glutaraldehyde 2.5% Aqueous	1 L	118.00	Add to Cart
0-5°C	16537-18-1G	Glutaraldehyde 2.5% Aqueous	1 Gal.	395.00	Add to Cart

Glutaraldehyde 3.0% Aqueous

0-5°C	16537-19	Glutaraldehyde 3% Aqueous	100 ml	28.00	Add to Cart
0-5°C	16537-20-S	Glutaraldehyde 3% Aqueous	500 ml	68.00	Add to Cart
0-5°C	16537-21	Glutaraldehyde 3% Aqueous	1 L	118.00	Add to Cart
0-5°C	16537-21-1G	Glutaraldehyde 3% Aqueous	1 Gal.	395.00	Add to Cart

Glutaraldehyde 3.0% Paraformaldehyde 3% in 0.1M Phosphate Buffer, pH 7.4

0-5°C	16538-06	Glutaraldehyde 3% Paraformaldehyde 3% in Phosphate Buffer, pH 7.4	10x10 ml	28.00	Add to Cart
0-5°C	16538-07	Glutaraldehyde 3% Paraformaldehyde 3% in Phosphate Buffer, pH 7.4	500 ml	68.00	Add to Cart
0-5°C	16538-08	Glutaraldehyde 3% Paraformaldehyde 3% in Phosphate Buffer, pH 7.4	1 L	98.00	Add to Cart
0-5°C	16538-08-1G	Glutaraldehyde 3% Paraformaldehyde 3% in Phosphate Buffer, pH 7.4	1 Gal.	350.00	Add to Cart

Glutaraldehyde 4% in 0.1M in Sodium Cacodylate Buffer, pH 7.4

0-5°C	16539-06	Glutaraldehyde 4% in 0.1M Sodium Cacodylate Buffer, pH 7.4	10x10 ml	40.00	Add to Cart
0-5°C	16539-07	Glutaraldehyde 4% in 0.1M Sodium Cacodylate Buffer, pH 7.4	500 ml	140.00	Add to Cart
0-5°C	16539-08	Glutaraldehyde 4% in 0.1M Sodium Cacodylate Buffer, pH 7.4	1 L	280.00	Add to Cart
0-5°C	16539-08-1G	Glutaraldehyde 4% in 0.1M Sodium Cacodylate Buffer, pH 7.4	1 Gal.	912.00	Add to Cart

Glutaraldehyde 5% Aqueous

0-5°C	16539-15	Glutaraldehyde 5% Aqueous	10x10 ml	27.00	Add to Cart
0-5°C	16539-20	Glutaraldehyde 5% Aqueous	500 ml	68.00	Add to Cart
0-5°C	16539-25	Glutaraldehyde 5% Aqueous	1 L	118.00	Add to Cart
0-5°C	16539-25-1G	Glutaraldehyde 5% Aqueous	1 Gal.	395.00	Add to Cart

Glutaraldehyde 5% in 0.1M Phosphate Buffer, pH 7.4

0-5°C	16539-30	Glutaraldehyde 5% in 0.1M Phosphate Buffer, pH 7.4	10x10 ml	40.00	Add to Cart
0-5°C	16539-35	Glutaraldehyde 5% in 0.1M Phosphate Buffer, pH 7.4	500 ml	139.00	Add to Cart
0-5°C	16539-40	Glutaraldehyde 5% in 0.1M Phosphate Buffer, pH 7.4	1 L	279.00	Add to Cart
0-5°C	16539-40-1G	Glutaraldehyde 5% in 0.1M Phosphate Buffer, pH 7.4	1 Gal.	910.00	Add to Cart

Glutaraldehyde/Formaldehyde

Fixative (Karnovsky's)
This fixative contains 3% glutaraldehyde and 2% formaldehyde in 0.1M phosphate buffer, pH 7.4.

Karnovsky's with formalin 2% and Biological glutaraldehyde 3% in 0.1m Phosphate Buffer.

0-5°C

15731-10

Karnovsky Fixative Reagent Grade

1 L

260.00

Add to Cart

Karnovsky's with paraformaldehyde solution and EM grade glutaraldehyde.

Please Note: This kit can be formulated to fit your requirements. Please contact us.

RT

15732-10

Karnovsky Fixative EM Grade

1 L

286.00

Add to Cart

Ampoule Breaker; Soft Silicone

Reusable – Made from soft silicone. It offers a simple way to break pre-scored glass ampoules safely. Accommodates 5, 7, and 10 ml size ampoules. Reduces injury and spillage.

60600

Ampoule Breaker

each

16.00

Add to Cart

Ampoule Cracker™

The Ampoule Cracker™ is a disposable plastic fracture and safety collar, which is specially designed to aid in the breaking open of pre-scored glass ampoules. Simply slip the Ampoule Cracker™ over the top at the pre-scored portion, snap it, and discard both the ampoule top and the safety collar to prevent cross contamination.

60605-04	Ampoule Cracker for Ampoule Size 1-4 ml	500/pk	41.00	Add to Cart
60605-10	Ampoule Cracker for Ampoule Size 5-10 ml	500/pk	41.00	Add to Cart
60605-20	Ampoule Cracker for Ampoule Size 10-20 ml	500/pk	77.00	Add to Cart

Break-Safe™ Ampoule Opener

EMS Introduces Break-Safe™ allowing you to safely open up to 3 ampoules at one time.

Box style design allows one handed opening of up to 3 ampoules at a time. Gently pressing down on the lid snaps ampoules necks clean while keeping them contained within the device and away from fingers. After removing ampoules contents, simply turn the device upside down over a trash receptacle to safely discard ampoules and tops.

Features

• Includes 4 inserts to allow opening of 1ml, 2ml, 5ml and 10 ml ampoules.
• Open ampoules easily and safely
• Transparency allows unobstructed viewing of ampoules contents at all times.
• Copolyester plastic construction has broad chemical resistance

60607

Break-Safe™ Ampoule Opener

each

50.00

Add to Cart

HISTOCHOICE™MB®* – The Molecular Biology Fixative

• SAFE – Contains no Formaldehyde, Glutaraldehyde or Mercury
• NON-TOXIC – Is completely odorless and can be safely disposed
  of down the drain

HISTOCHOICE™ MB® Tissue Fixative is the first fixative designed for the molecular biologist. It is specialty formulated to preserve antigenic sites for antibody probes and nucleic acid sites for immunohistochemistry. HISTOCHOICE™MB® replaces formaldehyde based, alcohol based, Zenkers, B-5, B-3, Bouin's and other fixatives with superior results. Tissue fixed in HISTOCHOICE™MB® exhibit vibrant staining, better nuclear and cytoplasmic detail, and will retain a crisp appearance even after long-term fixation.

Because this formula is designed specially for molecular biology applications, HISTOCHOICE™MB® fixed sections do not require pre-digestion or other recovery procedures to make important sites available.This means you spend less time preparing slides and more time doing research.

More than a fixative, HISTOCHOICE™MB® is a preservative that leaves antigens and nucleic acids in their native state, allowing binding for specific probes. Primary antibodies can often be diluted several-fold due to the increased number of preserved antigenic sites, saving cost on every slide you process.

HISTOCHOICE™MB® fixed tissue retains a much more natural look and feel than formalin fixed tissue. It is important to remember that these tissues are not fixed by formalin, but rather preserved by HISTOCHOICE™MB®.

HISTOCHOICE™MB® is on long-term backorder... please check back for updates.

RT	64115-01	HISTOCHOICE™MB® Fixative	1 L	60.00	Add to Cart
RT	64115-04	HISTOCHOICE™MB® Fixative	4 L	190.00	Add to Cart
RT	64115-44	HISTOCHOICE™MB® Fixative	4 x 4 L	695.00	Add to Cart

* HISTOCHOICE is registered name of Amresco

Hollande's Fixative

A modification of Bouin's solution. It is stable and will decalcify small bone specimens. Tissue that is fixed with Hollande's can be stained successfully with most stains, and the cupric acetate in the solution stabilizes red blood cell membranes and cosinophil and endocrine cell granules so that less lysis occurs than with Bouin's solution. Hollande's is widely used as a fixative for biopsy specimens of the gastrointestinal tract.

Thorough washing of the fixative prior to placing the specimen in a phosphate buffered formalin solution is necessary because the salts present in the solution will form an insoluble phosphate precipitate. Packaged in leak-proof histo-containers; prefilled ready-to-use.

	Catalog #	Cup Size	Filled	Pack/cs	Price
RT	64120-10	15 ml	10 ml	50	78.00	Add to Cart
RT	64120-20	30 ml	20 ml	50	98.00	Add to Cart
RT	64120-30	60 ml	30 ml	20	46.00	Add to Cart

Bulk packaging available

RT	64120-01	Hollande's Fixative	1 L	46.00	Add to Cart
RT	64120-04	Hollande's Fixative	4 L	168.00	Add to Cart
RT	64120-01-5G	Hollande's Fixative	5 Gal.	675.00	Add to Cart

McDowell Trumps Fixative

RT	18030-05	McDowell Trumps Fixative	500 ml	33.00	Add to Cart
RT	18030-10	McDowell Trumps Fixative	1 L	39.00	Add to Cart
RT	18030-10-1G	McDowell Trumps Fixative	1 Gal.	160.00	Add to Cart
RT	18030-10-5G	McDowell Trumps Fixative	5 Gal.	600.00	Add to Cart

Michel's Fixative, pH 7.0 – 7.4

RT	18040-05	Michel's Fixative	500 ml	50.00	Add to Cart
RT	18040-10	Michel's Fixative	1 L	85.00	Add to Cart
RT	18040-20	Michel's Fixative Pre-filled	20 x 10 ml	75.00	Add to Cart
RT	18040-10-1G	Michel's Fixative	1 Gal.	250.00	Add to Cart
RT	18040-10-5G	Michel's Fixative	5 Gal.	624.00	Add to Cart

Technical Tip:

Preparation of Tissue Biopsy in Michel's Fixative.

Michel's Transport Medium

Michel's Transport Medium allows for the shipping of specimens for further immunofluorescence work. It preserves morphology and antigenicity during transport. It is not a buffer or fixative. It is simply a vehicle to transport samples. The solution must be stored at refrigerated temperatures. Do not freeze. Samples may be stored up to 5 days in this medium at room temperature. Can be used with Michel's Buffer (washing solution).

4-8°C	18042-05	Michel's Transport Medium	250 ml	28.00	Add to Cart
4-8°C	18042-10	Michel's Transport Medium	500 ml	48.00	Add to Cart
4-8°C	18042-15	Michel's Transport Medium	1 L	65.00	Add to Cart
4-8°C	18042-16	Michel's Transport Medium	1 gal.	142.00	Add to Cart
4-8°C	18042-17	Michel's Transport Medium, 15ml fill in SecurTainer™	50/cs	80.00	Add to Cart
4-8°C	18042-16-5G	Michel's Transport Medium	5 Gal.	625.00	Add to Cart

Mirsky's Fixative™

• Saponin/Glyoxylate Fixative
• Preserves Immunohistological activity
• Contains no formaldehyde
• Fast-acting

Mirsky's Fixative™ is a superior fixing agent for use in immunohistological and immunological protocols. Mirsky's contains no formaldehyde or glutaraldehyde. Therefore, it has considerably reduced toxicity and virtually no odor. Mirsky's Fixative is neutral, buffered, and isotonic (308 mOsmol). Additionally, Mirsky's Fixative does not contain toxic or hazardous buffers such as cacodylate or barbital. Hardening or shrinkage of tissue is considerably reduced.

Double reactive sites afford excellent cross-linking properties while maintaining sample enzyme activity. Samples processed in Mirsky's Fixative for light microscopy can subsequently be used in electron microscopy procedures. Special buffer systems may be used in place of the buffer provided.
Use as replacement for formalin and/or glutaraldehyde fixatives in immunohistological and immnocytological staining protocols. Tissue size is unlimited, as long as the sample is no thicker than 0.5cm in at least one plane to assure uniformity of tissue penetration. The gross visual appearance will be different than in formaldehyde and tissue may seem "raw". This is due to double site binding of the fixative with a resultant reduction in tissue shrinkage. Microscopic examination of tissue morphology will be noticeably improved. This material is intended to maintain enzyme and antibody activity. For best results, tissue section of high digestive enzyme content should be thoroughly rinsed in saline solution before fixation (e.g. trypsin in intestinal samples). Once fixed, tissue may be retained in Mirsky's Fixative and ethanol indefinitely.

Mirsky's Fixative is normally distributed as a concentrated two bottle system, although high through-put laboratories often use the single bottle ready-to-use format. The two bottle system is comprised of Mirsky's Fixative 10X Concentrated and Mirsky's Fixative 10X Buffer. To reconstitute to working strength, add 1 part Mirsky's Fixative 10X Buffer to 8 parts distilled or deionized water, mix thoroughly, then add 1 part Mirsky's Fixative 10X Concentrate and mix again.

The two bottle system has a shelf life of 12 months while the ready-to-use format has a shel life of 30 days.

RT	15560	Mirsky's Fixative System		200 ml	42.00	Add to Cart
RT	15561-05	Mirsky's Fixative Ready-to-Use in Ethanol		500 ml	24.00	Add to Cart
RT	15561-10	Mirsky's Fixative Ready-to-Use in Methanol		1L	42.00	Add to Cart
RT	15561-20	Mirsky's Fixative Ready-to-Use in Solvent of your choice		2L	82.00	Add to Cart

Russell's Zenker Fixative (Russel's Modified Zenker)

Due to the hazardous nature of mercury, Zenker's fluid is no longer available. In its place we are offering Russell's Zenker Fixative, which contains Zinc Chloride. The staining results produced by this fixative are comparable to those of Zenker's Stock solution. Russell's Modified Zenker's is used in the same manner as Zenker's stock solution.

RT	64123-05	Russell's Zenker Fixative	500 ml	27.00	Add to Cart
RT	64123-06	Russell's Zenker Fixative	1 L	46.00	Add to Cart
RT	64123-06-1G	Russell's Zenker Fixative	1 Gal.	160.00	Add to Cart
RT	64123-06-5G	Russell's Zenker Fixative	5 Gal.	495.00	Add to Cart

Formic Acid 96%, Reagent, A.C.S.

HCOOH   F.W. 46.03   CAS #64-18-6
Specific Gravity: 1.22

RT	15750	Formic Acid, 96%	225 ml	19.00	Add to Cart
RT	15760	Formic Acid, 96%	1 liter	50.00	Add to Cart

Gold Chloride, Hydrate(Chloroauric Acid; Hydrogen Tetrachloroaurate)

H(AuCl₄)·H₂O   F.W. 357.79   CAS #16961-25-4
Assay 99.9985%
Gold contents 49%+
A highly purified gold compound for the preparation of Colloidal Gold sols. Preweighed to facilitate your work. (Preparation instructions for Gold Sols included).

RT	16580		10 x 1/10g	159.50	Add to Cart
RT	16582		10 x ½g	650.00	Add to Cart
RT	16583		1g	152.00	Add to Cart
RT	16584		10x1g	1,395.00	Add to Cart

Gold Chloride Aqueous Solutions

RT	16586	Gold Chloride, 0.1% Aqueous		500 ml	72.00	Add to Cart
RT	16587	Gold Chloride, 0.2% Aqueous		500 ml	112.00	Add to Cart
RT	16588	Gold Chloride, 0.5% Aqueous		500 ml	226.00	Add to Cart
RT	16589	Gold Chloride, 1.0% Aqueous		500 ml	506.00	Add to Cart
RT	16590	Gold Chloride, 10.0% Aqueous		500 ml	3,995.00	Add to Cart

Gold, Immuno, see Section 2 ImmunoGold Reagents

Gum Mastic

0-4°C	16590-01	Gum Mastic, 2.5% in Alcohol		500 ml	111.00	Add to Cart
0-4°C	16591	Gum Mastic, 5% in Alcohol		500 ml	116.00	Add to Cart
0-4°C	16592	Gum Mastic, 10% in Alcohol		500 ml	143.00	Add to Cart
0-4°C	16595	Gum Mastic, 10% in Isopropanol		500 ml	143.00	Add to Cart

Hexamethylenetetramine, A.C.S. Reagent, Granular

(Urotropine; Methenamine)

C₆N₄   F.W. 140.19   CAS #100-97-0

Assay:

99.0-100.5%

Specifications

Residue after Ignition		0.1%
Chloride		0.014%
Sulfate		To Pass Test
Heavy Metals.		0.001%

Used in silver stains.

Swift, J.A. (1968) Microsc. Soc. 88, 449.

RT	16710		50g	14.00	Add to Cart
RT	16710-25*		25g	8.50	Add to Cart
RT	16712	Methynamine, 3% Aqueous	500 ml	25.00	Add to Cart

Hexylene Glycol, Reagent, A.C.S.

(2-Methyl-2,4-pentanediol)

(CH₃)₂C(OH)CH₂CH(OH)CH₃   F.W. 118.18   CAS #107-41-5

Specific Gravity @25°C 0.923

A.C.S. Specifications

Assay		99%
IR Spectrum		To Pass Test
Refractive Index @20°C		0.921-0.923

Mixed with water in all proportions. Used as a dehydrating agent for fat tissues.

RT	16740		450 ml	27.00	Add to Cart
RT	16750		4 x 450 ml	99.00	Add to Cart

Reagents: HISTOCHOICE to Zinc/Formalin