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Electron Microscopy Sciences

Histology and Light Microscopy

arrow13Reagents for Histology: Fixing, Clearing, and Dehydrating

EMS is now offering a complete line of reagents for Histopathology:The highest quality for Fixing, Dehydrating/Clearing and Staining.

arrowAcetate Buffer

RT 11482-42 Acetate Buffer, 0.2M, pH 4.2 msds 500 ml 22.00 Add to Cart
RT 11482-50 Acetate Buffer, 0.2M, pH 5.0 msds 500 ml 22.00 Add to Cart
RT 11482-56 Acetate Buffer, 0.2M, pH 5.6 msds 500 ml 22.00 Add to Cart

msdsarrowAcetic Acid, Glacial, Reagent A.C.S

C2H4O2 F.W. 60.05 CAS 64-19-7

Assay min   99.7%
RT 10040 Acetic Acid, Glacial 500 ml 59.00 Add to Cart
RT 10041 Acetic Acid, Glacial 2.5 L 120.00 Add to Cart

arrowAcetic Acid, Aqueous Solution

Prepared from acetic acid glacial with de-ionized water.

RT 10042-02 Acetic Acid, 0.2% (v/v) Solution msds 500 ml 19.00 Add to Cart
RT 10042-05 Acetic Acid, 0.5% (v/v) Solution msds 500 ml 20.00 Add to Cart
RT 10042-10 Acetic Acid, 1.0% (v/v) Solution msds 500 ml 22.00 Add to Cart
RT 10042-50 Acetic Acid, 5.0% (v/v) Solution msds 500 ml 30.00 Add to Cart

msdsarrowAcetone, Reagent, A.C.S.

CH3COCH3   F.W. 58.08   CAS #67-64-1

A.C.S. Specifications

Assay   Min 99.5%
Aldehyde (as HCHO)   0.002%
Color   Max. APHA 10
Density (G/ml) at 25°C   Max. 0.7857
Titratable Acid   0.0003 meq/g
Titratable Base   0.0006 meq/g
Isopropyl Alchohol   0.05%
Methanol (CH3OH)   0.05%
Residue after Evaporation   0.001%
Solubilty in water   To pass test
Substances Reducing
Permanganate		   To pass test
Water   0.5%
RT 10000   1 qt. (946 ml) 14.00 Add to Cart
RT 10010   4 x 1 qt. 49.00 Add to Cart
RT 10012   250 ml 8.00 Add to Cart
RT 10014   4 x 250 ml 26.00 Add to Cart

arrowAcetone: Alcohol, 1:1 & Buffered Acetone

RT 10017 Acetone: Alcohol, 1:1 msds 500 ml 21.00 Add to Cart
RT 10018 Buffered Acetone Fixative msds 500 ml 25.00 Add to Cart

arrowAcid Alcohols

An acidified alcohol solution for the differentiation of regressive stains.

RT 26071-01 Acid Alcohol, 0.5% HCl in 70% Alcohol msds 1 L 37.00 Add to Cart
RT 26071-04 Acid Alcohol, 0.5% HCl in 70% Alcohol 4 x 1 L 138.00 Add to Cart
RT 26072-01 Acid Alcohol, 1% HCl in 70% Alcohol msds 1 L 33.00 Add to Cart
RT 26072-04 Acid Alcohol, 1% HCl in 70% Alcohol 4 x 1 L 120.00 Add to Cart
RT 26073-01 Acid Alcohol, 1% HCl in 95% Alcohol msds 1 L 43.00 Add to Cart
RT 26073-04 Acid Alcohol, 1% HCl in 95% Alcohol 4 x 1 L 160.00 Add to Cart

msdsarrow12Ethyl Alcohol, Specially Denatured

C2H5OH   F.W. 46.07   CAS #64-17-5

Blended 95% Ethanol and 5% Methanol.

Suitable for all routine histology/cytology preparations.

Specifications

Evaporation Residue   5ppm max.
Water   0.1%
Filtered SDA-3A   95%
RT 15050   1 qt 25.00 Add to Cart
RT 15051   4 x 1 qt 82.00 Add to Cart

msdsarrow12Ethyl Alcohol, Anhydrous

(Ethanol) – 200 Proof
CH3CH2OH   F.W. 46.07   CAS # 64-17-5

Specifications

Specific Gravity <0.7942
Purity >99.9%
Acidity <0.002%
Water (Karl Fisher) <0.1%
Non-Volatile Matter <0.0025%
RT 15055   450 ml 22.00* Add to Cart
RT 15056   4 x 450 ml 72.00* Add to Cart
RT 15058   1 liter 36.00 Add to Cart

*Tax has already been paid

arrow12Alkaline Alcohol

RT 10380 10% NH4OH in 85.5% Alcohol msds 500 ml 24.00 Add to Cart
RT 10381 1.6% NaCl in 80% Alcohol msds 500 ml 39.00 Add to Cart
RT 10382 0.2% KOH in 80% Alcohol msds 500 ml 40.00 Add to Cart

msdsarrowAmmonium Hydroxide, 28% Reagent, A.C.S.

NH4OH   F.W. 35.05   CAS #1336-21-6 Specific Gravity: 0.91.

Assay   28-30%

Specifications

Residue After Ignition   0.002%
Carbon Dioxide   0.002%
Chloride   0.5 ppm
Phosphate   2 ppm
Sulfate   2 ppm
Heavy Metals   0.5 ppm
Iron   0.2 ppm
RT 10600   450 ml 19.00 Add to Cart
RT 10601   4 x 450 ml 70.00 Add to Cart

msdsarrow11Amphibian Ringer Solution

Ingredients: NaCl; KCl; CaCl2·6H2O and Distilled water.

RT 11762-05 Amphibian Ringer Solution 500 ml 25.00 Add to Cart
RT 11762-10 Amphibian Ringer Solution 1 L 35.00 Add to Cart

msdsarrow11B-5 Fixative

For lymph nodes.

RT 64122-05 B-5 Fixative 500 ml 64.00 Add to Cart
RT 64122-06 B-5 Fixative 1 lt 120.00 Add to Cart
RT 64122-10 B-5 Fixative 4 lt 450.00 Add to Cart

arrow12Borate Buffer

Please see the Buffer Section.

arrow12msdsBouin's Solution

Bouin's solution can be used as a fixation and a staining fluid. Bouin's fixative is excellent for use on biopsy specimens of the gastrointestinal tract. Tissue from the endocrine system are well fixed and many antibodies react well with tissue fixed in Bouin's. Bouin's solution may be used as a routine fixative or a staining solution, but should not be used as a preservative for tissue. Tissue cannot be held in Bouin's solution for extended periods. The maximum fixation time in this solution should be less than 48 hours and should be transferred to alcohol.

Packaged in leak-proof histo-containers, prefilled and ready-to-use.

Formulated with Picric Acid saturated aqueous solution 75%, Formalin (40% aqueous formaldehyde) 25% and Glacial Acetic acid 5% in de-ionized water.

RT 15990-01 Bouin's Fluid 1 L 47.00 Add to Cart
RT 15990-04 Bouin's Fluid 4 L 163.00 Add to Cart
RT 15990-10 Bouin's Fluid Pre-filled cups 50x10 ml/cs 83.00 Add to Cart
RT 15990-20 Bouin's Fluid Pre-filled cups 50x20 ml/cs 103.00 Add to Cart
RT 15990-30 Bouin's Fluid Pre-filled cups 20x30 ml/cs 47.00 Add to Cart

arrowBuffers

arrowAcetate Buffer

RT 11482-42 Acetate Buffer, 0.2M, pH 4.2 msds 500 ml 23.00 Add to Cart
RT 11482-50 Acetate Buffer, 0.2M, pH 5.0 msds 500 ml 23.00 Add to Cart
RT 11482-56 Acetate Buffer, 0.2M, pH 5.6 msds 500 ml 23.00 Add to Cart

msdsAraldite 502arrowAntibody Dilution Buffer with Immunoglobulin-Free BSA

This ready-to-use Antibody Dilution Buffer is designed for the dilution of antibodies in all immunoassays, including immunofluorescence, IHC, ELISA, WB. This buffer contains immunoglobulin free BSA and does not contain phosphate, sodium azide, or mercury preservatives. It can be used for the dilution of all antibodies, including peroxidase, and antibodies to phophoproteins. However, it is not suitable for dilution of antibodies to S100 proteins. This buffer contains green food color.

Reagent Supplied: Ready to use buffer.

Application: Dilute antibodies, primary or secondary as recommended in the protocol by the manufacturer of these products.

Storage: 2-8°C. Diluted antibodies can be stored at 2-8°C for several weeks, depending upon the stability of antibodies.

2–8°C 25886-01 Antibody Dilution Buffer, free BSA 100 ml 62.00 Add to Cart
2–8°C 25886-05 Antibody Dilution Buffer, free BSA 500 ml 176.00 Add to Cart
  1227SK Antigen Preserve Solution VII 1 L 154.00 Add to Cart

arrow11Borate Buffer

RT 11455-78 Borate Buffer, pH 7.8 msds 500 ml 33.00 Add to Cart
RT 11455-82 Borate Buffer, pH 8.2 msds 500 ml 34.00 Add to Cart
RT 11455-90 Borate Buffer, pH 9.0 msds 500 ml 34.00 Add to Cart

msdsarrow12Citrate Buffer

Citric acid and Sodium citrate
0.1M
pH range 4.6-6.2.

0-5°C 11490 Citrate Buffer, 0.1M 225 ml 18.00 Add to Cart
0-5°C 11492 Citrate Buffer, 0.1M 500 ml 23.00 Add to Cart

msdsarrow11Michel's Buffer

RT 18041-05 Michel's Buffer 500 ml 22.00 Add to Cart
RT 18041-10 Michel's Buffer 1 L 29.00 Add to Cart

msdsarrow12Millonig's Phosphate Buffer

Millonig (1964); Karlsson and Schultz (1965)
Ingredients:
Sodium Phosphate, monobasic (NaH2PO4xH2O)
Sodium Phosphate, dibasic (Na2HPO4xH2O)
0.5% Sodium Chloride (NaCl)
Shipping Conditions: Ambient Temperature

The pH of this buffer is 7.4 (0.1M); the osmolarity is 440 mosmols, so this buffer is hypertonic to most body fluids. Millonig recommended to use this buffer for the fixation of very hydrated tissues with osmium tetroxide. For marine organisms, a higher concentration of sodium chloride (3%) is recommended. We supply it in a double strength (0.2M), pH 7.4. Dilution is needed before use.

0-5°C 11582-05 Millonig's Phosphate Buffer 0.2M 500 ml 23.00 Add to Cart
0-5°C 11582-10 Millonig's Phosphate Buffer 0.2M 1 L 32.00 Add to Cart

msdsmsdsarrow12PHEM Buffer

EMS is proud to announce and release of our newest Buffer for EM PHEM.

PHEM buffer has been used primarily for tissues and cell cultures being processed for immunocytochemical studies.  Most antigens, especially intra cellular ones, stain better using PHEM than those processed using PBS, it also has a more limited effect on biochemical reactions and enzymes. Very good preservation of cellular ultrastructure, notably microtubules, is also an advantage of PHEM use.

References

  1. Schliwa, Manfred, and Jonathan Van Blerkom. "Structural interaction of cytoskeletal components." The Journal of Cell Biology 90.1 (1981): 222-235.
  2. Jacqueline Montanaro: Improved ultrastructure of marine invertebrates using non-toxic buffers
Academic Editor: James Reimer, published online March 31, 2016
2-8°C 11162 PHEM Buffer, 0.1M 225ml 42.00 Add to Cart
2-8°C 11163 PHEM Buffer, 0.1M 500ml 74.00 Add to Cart
2-8°C 11164 PHEM Buffer, 0.2M 225ml 48.00 Add to Cart
2-8°C 11165 PHEM Buffer, 0.2M 500ml 82.00 Add to Cart

arrow12Phosphate Buffer, 0.1M*

Prepared from Sodium Phosphate Monobasic Hydrate and Sodium Phosphate Dibasic Heptahydrate. Non-Sterile. Meant for research purposes only.

  19340-60 Phosphate Buffer, pH 6.0 msds 1 L 40.00 Add to Cart
  19340-65 Phosphate Buffer, pH 6.5 msds 1 L 40.00 Add to Cart
  19340-70 Phosphate Buffer, pH 7.0 msds 1 L 40.00 Add to Cart
  19340-72 Phosphate Buffer, pH 7.2 msds 1 L 40.00 Add to Cart

*Meant for biological research only. Not to be used for medical or any other purposes.

arrow12Phosphate Buffer Saline (PBS)

PBS is commonly used in biochemistry. It is a salty solution containing sodium chloride, potassium chloride, sodium phosphate and potassium phosphate. PBS is isotonic and non-toxic to cells. 1X PBS final concentration is 8.0 g/L NaCl, 0.2 g/L KCl, 1.42 g/L Na2HPO4, 0.24 g/L KH2PO4 and pH 7.4.

RT 19342-10 Phosphate Buffered Saline, 10X msds 1 L 32.00 Add to Cart
RT 19242-40 Phosphate Buffered Saline, 10X 1 gal 98.00 Add to Cart
RT 19343-10 Phosphate Buffered Saline, 2X msds 1 L 28.00 Add to Cart
RT 19343-40 Phosphate Buffered Saline, 2X 1 gal 88.00 Add to Cart
RT 19344-10 Phosphate Buffered Saline, 1X msds 1 L 24.00 Add to Cart
RT 19344-20 Phosphate Buffered Saline, 1X 1 gal 76.00 Add to Cart

arrow12Sorensen's Phosphate Buffer

A Phosphate buffered mixed salt prepared from sodium phosphate monobasic and sodium phosphate dibasic.

0-5°C 11600-10 Sorensens Phosphate Buffer, 0.2M, pH 7.2 msds 1 L 32.00 Add to Cart
0-5°C 11600-40 Sorensens Phosphate Buffer, 0.2M, pH 7.2 4 L 110.00 Add to Cart
0-5°C 11601-10 Sorensens Phosphate Buffer, 0.2M, pH 7.4 msds 1 L 33.00 Add to Cart
0-5°C 11601-40 Sorensens Phosphate Buffer, 0.2M, pH 7.4 4 L 110.00 Add to Cart

arrow12Tris, (hydroxymethyl) aminomethane maleate

msdsTris Maleate Buffer
0-5°C 11730 Tris Maleate Buffer 0.2M pH range 6.4-8.0 250 ml 23.00 Add to Cart
0-5°C 11740 Tris Maleate Buffer 0.2M pH range 6.4-8.0 500 ml 30.00 Add to Cart
msdsTris Buffer
RT 11730-05 Tris Buffer 0.2M pH 8.0 250 ml 23.00 Add to Cart
RT 11730-06 Tris Buffer 0.2M pH 8.0 500 ml 30.00 Add to Cart
msdsTris Buffered Saline
RT 11730-08 Tris Buffered Saline pH 7.4 250 ml 23.00 Add to Cart
RT 11730-09 Tris Buffered Saline pH 7.4 500 ml 30.00 Add to Cart
msdsTris-HCL Buffer
RT 11730-12 Tris-HCL Buffer 1M pH range 6-8 250 ml 23.00 Add to Cart
RT 11730-15 Tris-HCL Buffer 1M pH range 6-8 500 ml 30.00 Add to Cart
msdsTris HCL Buffer
RT 11730-20 Tris HCL Buffer pH range 7.2-9.0 250 ml 23.00 Add to Cart
RT 11730-25 Tris HCL Buffer pH range 7.2-9.0 500 ml 30.00 Add to Cart

msdstechnical data sheetarrow12Universal Immunoassay Buffer™, 10X

Immunoassay buffer is used as a 1X in all immunoassays (immunofluorescence, IHC, ELISA, WB etc.) as a washing buffer and it can be used as a dilution buffer for antibodies; however it does not contain any carrier proteins.

This buffer also does not contain any phosphate, sodium azide or mercury as preservatives and can be used for dilution of all antibodies, including peroxidase, antibodies to phosphoproteins. It is not suitable for dilution of antibodies to S100 proteins.

Reagent Supplied: 10X Buffer.

Application: Dilute this 10X buffer 10 times with DI water. (90 ml water + 10 ml this buffer – mixed well). Wash 3-5 times with this buffer in between the steps of your immunoassay protocol.

Storage: Room temperature.

RT 25880-01 Universal Immunoassay Buffer, 10X 100 ml 60.00 Add to Cart
RT 25880-10 Universal Immunoassay Buffer, 10X 1 L 160.00 Add to Cart

msdstechnical data sheetarrow12Universal Antibody Dilution Buffer™

This ready-to-use Universal Antibody Dilution Buffer is designed for dilution of antibodies in all immunoassays, including immunofluorescence, IHC, ELISA, WB. This buffer does not contain any mammalian proteins, phosphate, sodium azide, or mercury preservatives and can be used for dilution of all antibodies, including peroxidase, and antibodies to phophoproteins. However, it is not suitable for dilution of antibodies to S100 proteins.

Reagent Supplied: Ready to use buffer.

Application: Dilute antibodies, primary or secondary as recommended in the protocol by the manufacturer of these products.

Storage: 2-8°C. Diluted antibodies can be stored at 2-8°C for several weeks, depending upon the stability of antibodies.

2-8°C 25885-01 Universal Antibody Dilution Buffer 100 ml 60.00 Add to Cart
2-8°C 25885-05 Universal Antibody Dilution Buffer 500 ml 165.00 Add to Cart

msdsarrown-Butyl Alcohol, Reagent, A.C.S.

(Butanol)
CH3CH2CH2CH2OH   F.W. 74.12   CAS # 71-36-3

Specific Gravity:   0.81
Assay   99.4%
Residue after evaporation   0.005%
Titratable acid   0.0008meq/g
Butyl Ether   0.2%
Water   0.1%
RT 11920   450 ml 30.00 Add to Cart
RT 11921   4x450 ml 104.00 Add to Cart

arrow12Carnoy's Fixatives

Stock solution.

RT 64130-05 Carnoy's Fluid msds 500 ml 26.00 Add to Cart
RT 64130-50 Carnoy's Fluid Without Chloroform msds 500 ml 24.00 Add to Cart

msdsarrow12Cedar Wood Oil

CAS #8000-27-9 Refractive Index @ 20°C 1.504+/-.002

For clearing.

RT 12420   100 ml 66.00 Add to Cart

msdsarrow12Chloroform, Reagent, A.C.S.

CHCl3   FW 119.38   CAS #67-66-3

A.C.S. Specifications

Color (APHA)   10
Density @ 25°C   1.471-1.476
Residue after Evaporation   0.001%
Acetone & Aldehyde (about 0.00%)   To Pass Test
Acid & Chloride   To Pass Test
Free Chlorine (Cl)   To Pass Test
Lead (Pb)   0.05ppm
Subs. darkened by H2SO4   To Pass Test
Suitability for Dithizone Test   To Pass Test
RT 12540   450 ml 20.00 Add to Cart
RT 12541   4x450 ml 78.00 Add to Cart

msdsarrow12Chromic Acid, Reagent, Crystals, A.C.S.

CrO3   FW 99.99   CAS #1333-82-0

A.C.S. Specifications

Chloride (Cl)   0.005%
Insoluble Matter   0.01%
Nitrate (NO3)   0.05%
Sodium (Na)   0.2%
Iron, Aluminum, Barium   0.03%
Sulfate (SO4)   0.05%
Assay   98%
RT 12555   100g 45.00 Add to Cart

arrow11Chromic Acid 4% Aqueous Solution

RT 12557 Chromic Acid 4% Aqueous Solution msds 500 ml 30.00 Add to Cart
RT 12558 Chromic Acid 5% Aqueous Solution msds 500 ml 34.00 Add to Cart

msdstechnical data sheetarrow11Clearing Solvent Xylene Substitutes

These clearing solvents offer Histology and Cytology laboratories a safer alternative to xylene.

  • Contains no xylene or toluene
  • No citrus odor
  • For use in manual and automated systems
RT 64129-10 Clearing Solvent 1 gal 52.00 Add to Cart

RDO Rapid Decalcifierarrow11RDO Rapid Decalcifier

RDO and RDO Gold were developed with the basic premise of making your work routine quicker and easier without sacraficing the quality and integrity of your samples.

RDO and RDO Gold are remarkably effective multipurpose decacifiers that can be tailored to suit your specific lab routine.

Both RDO and RDO Gold

  • Offer crisp nuclear staining and definition
  • Give distinguishable chromatin
  • Show great surface decalcification
  • Safe enough to be disposed down to lab sink to drain

How is RDO Gold different from the original RDO

  • RDO Gold is designated for those biopsies which contain minimal amounts of calcium
  • No filtration is required with RDO Gold
  • RDO Gold gives stellar morphological representation of bone biopsies
  • There is no folding of tissues with RDO Gold
  • RDO Gold is clear and non-precipitate

Both RDO and RDO Gold are

  • Far Superior to other Decalcifiers
  • Great Cellular Differentiation
  • Safe – Non Toxic – Non-Corrosive
  • Dilutable
  • Microwavable
  • Superb Staining Quality
  • Biodegradable
  • Translucent
  • Available in Wide Mouth Liter Bottle

References

  1. Gayle Callis et al. "Dacalcification of Bone: Literature Review and Practical Study of Various Decalcifying Agents, Methods, and Their Effects on Bone Histology". The J. of Histology, Vol. 21, No. 1, March 1998
  2. Sherley A. Powell, HT (ASCP) HTL. "Method to Facilitate Sectioning Incompletely Decalcified Bone". Mercer University, School of Medicine. Microtime Georgia, State Newsletter
  3. Becky Scholes, HTL, MT (ASCP).:Bone Marrow Biopsies". H.I.S.T.O. Iowa State Newsletter
RT 64143-01 RDO Decalcifier msds 1 L 26.00 Add to Cart
RT 64143-06 RDO Decalcifier 6 x 1 L/cs 126.00 Add to Cart
RT 64143-40 RDO Decalcifier 1 gal 56.00 Add to Cart
RT 64143-44 RDO Decalcifier 4 x 1 gal/cs 195.00 Add to Cart
RT 64144-01 RDO – Gold Decalcifier msds 1 L 44.00 Add to Cart
RT 64144-06 RDO – Gold Decalcifier 6 x 1 L/cs 245.00 Add to Cart
RT 64144-40 RDO – Gold Decalcifier 1 gal 84.00 Add to Cart
RT 64144-44 RDO – Gold Decalcifier 4 x 1 gal/cs 299.00 Add to Cart

Technical Tips and Articles

RDO Rapid Decalcifier

Animal Tissue Techniques
Parafin and Plastic Techniques for Preparation of Bone Marrow Specimen
Bone Marrow Biopsies

msdsAlcoholic Stain Solutionsarrow11Decalcifier

  • Ready-to-use
  • For hard specimens
  • Protects cellular structure
  • Minimal interference with subsequent staining
  • For use with human and animal calcified tissue, bone, cartilage and other hard specimens
RT 64147-01 Decalcifier 1 L 32.00 Add to Cart
RT 64147-06 Decalcifier 6 x 1 L 138.00 Add to Cart
RT 64147-10 Decalcifier 10 L 179.00 Add to Cart

msdsarrowDeionized Water, Reagent Grade A.C.S.

H2O F.W. 18.02 CAS #7732-18-5
EMS Reagent Grade Water is typically prepared at 18 megohm/cm specific resistance using a reverse osmosis, mixed deionization, activated filtration and final filtration at 0.2 microns.

Color (APHA)   <+/-5
Bacteriological purity   0 CFUs/L
Residue after evaporation   10ppm
Coliform   negative
RT 22800-01 Deionized Water 1 gal 12.00 Add to Cart
RT 22800-05 Deionized Water 5 gal 50.00 Add to Cart

Training ManualTraining ManualEMS Rapid Pro TDSEMS Rapid Pro Processing SystemarrowEMS Rapid Pro Processing System

A Revolutionary Rapid Tissue Processing System

Tissue processing has not changed in more than a century... until now. The EMS Rapid Pro Processing System can cut total processing time to as little as 46 minutes.

Advantages over traditional processing

  • Speed – no other processing chemicals will deliver results this fast
  • Increased throughput
  • Suitable for both fresh and frozen sections
  • Catalyzed versions of standard processing chemicals eliminate the need for new safety features, storage, or learning curves
  • Effective for manual or automatic tissue processing
  • Compatible with existing tissue processing equipment
  • "Results While You Wait" option for patients

For more staining protocols, see the Technical Data Sheet.

A proven and reliable method for obtaining rapid results in tissue processing

Process Standard Processing Time EMS Rapid Pro Processing Time
Formalin ½ hr - 1 hr 3 min
70% Ethanol 1 hr 3 min
95% Ethanol 1 hr 3 min
98% Ethanol 1 hr 3 min
90% Xylenes ½ hr - 1 hr 3 min
98% Ethanol 1 hr 3 min
90% Xylenes ½ hr - 1 hr 3 min
Paraffin 2 - 3 hrs 25 min
Total Processing TIme 7½ - 10 hrs 46 min

Ordering

26750-10 EMS Rapid ProKit   kit 36.00 Add to Cart
26750-01 EMS Rapid Pro Solution 1 msds 250 ml 12.00 Add to Cart
26750-02 EMS Rapid Pro Solution 2 msds 250 ml 11.00 Add to Cart
26750-03 EMS Rapid Pro Solution 3 msds 250 ml 4.00 Add to Cart
26750-04 EMS Rapid Pro Solution 4 msds 250 ml 4.00 Add to Cart
26750-05 EMS Rapid Pro Solution 5 msds 250 ml 9.00 Add to Cart

arrow12Ferric Ammonium Sulfate

RT 15505-40 Ferric Ammonium Sulfate, 4% Aqueous msds 500 ml 31s.00 Add to Cart
RT 15505-50 Ferric Ammonium Sulfate, 5% Aqueous msds 500 ml 32.50 Add to Cart

msdsarrow12Ferric Chloride, Hexahydrate, Reagent, A.C.S.

FeCl3·6H2O   F.W. 270.30   CAS #13478-10-9

Specifications

Assay 97.0-102%
Insoluble Matters. 0.01%
Nitrate (about 0.001%) To Pass Test
Phosphorous Compound 0.01%
Sulfate 0.01%
Arsenic 0.002%
Copper 0.003%
Zinc 0.003%

References for use

Membrane stains

  • Basic, G., Berwick et al (1963). Hale stain for sialic acid containing mucins. J. Cell Biol., 19:223
  • Gasic, G., Berwick, et al (1968). Positive and Negative colloidal iron as cell surface electron stains. Lab. Invest., 18:63
  • Blanquet, P.R. and Loiez, A. (1974). Colloidal iron used at pHs lower than 1 as electron stain for surface proteins. J. Histochem. Cytochem., 22:368
  • Matukafs, V.J., Panner et al (1967). Studies on ultrastructural identification and distribution of protein-polysaccharides in cartilage matrix. J. Cell Biol., 32:365
  • Benedetti, E.L. and Emmelot, P. (1967). Studies on plasma membranes IV. The Ultrastructural localization and content of sialic acid in plasma membranes isolated from rat liver and hepatoma. J. Cell Sci., 2:499
  • Rowley, J.R. (1971). Resolution of channels in the exine by translocation of colloidal iron. Pro. 29th Ann. EMSA Meet., p.352. Claitor's Pub. Division, Baton Rouge, LA
  • Nicolson, G.L. (1973). Anionic sites of human erythrocyte membranes.I. Effects of trypsin, phospholipase C, and pH on the topography of bound positively charged colloidal particles. J. Cell Biol., 57:373
  • Hendy, R. (1971). Electron microscopy of lipofucsin pigment stained by the Schmorl and Fontana technique. Histochemie, 26:311
RT 15510 Ferric Chloride, Hexahydrate, Reagent 500g 45.00 Add to Cart

arrow12Ferric Chloride 10%, Aqueous Solution

RT 15512-02 Ferric Chloride 2% Aqueous msds 500 ml 37.00 Add to Cart
RT 15512-10 Ferric Chloride 10% Aqueous msds 500 ml 37.00 Add to Cart

Fixatives

msdsarrow12B-5 Fixative

For lymph nodes.

RT 64122-05 B-5 Fixative 500 ml 64.00 Add to Cart
RT 64122-06 B-5 Fixative 1 lt 120.00 Add to Cart
RT 64122-10 B-5 Fixative 4 lt 450.00 Add to Cart

msdsarrow11Bouin's Solution

Bouin's solution can be used as a fixation and a staining fluid. Bouin's fixative is excellent for use on biopsy specimens of the gastrointestinal tract. Tissue from the endocrine system are well fixed and many antibodies react well with tissue fixed in Bouin's. Bouin's solution may be used as a routine fixative or a staining solution, but should not be used as a preservative for tissue. Tissue cannot be held in Bouin's solution for extended periods. The maximum fixation time in this solution should be less than 48 hours and should be transferred to alcohol.

Packaged in leak-proof histo-containers, prefilled and ready-to-use.

Formulated with Picric Acid saturated aqueous solution 75%, Formalin (40% aqueous formaldehyde) 25% and Glacial Acetic acid 5% in de-ionized water.

RT 15990-01 Bouin's Fluid 1 L 47.00 Add to Cart
RT 15990-04 Bouin's Fluid 4 L 163.00 Add to Cart

Bouin's Fluid in Pre-filled cups.

RT 15990-10 Bouin's Fluid 50 x 10 ml/cs 83.00 Add to Cart
RT 15990-20 Bouin's Fluid 50 x 20 ml/cs 103.00 Add to Cart
RT 15990-30 Bouin's Fluid 20 x 30 ml/cs 47.00 Add to Cart

msdsarrow11Brasil Fixative

Ethanol, Picric Acid, Formalin, Acetic Acid, Glacial.
This fixative is most popular when glycogen is the target. The solution is similar to Bouins Solution (alcoholic) offering quick hardening and good staining with acid dyes.

RT 15995-10 Brasil Fixative 10x10 ml 42.00 Add to Cart
RT 15995-20 Brasil Fixative 100 ml 42.00 Add to Cart

msdsarrowBuffered Ethanol Fixative, 70% BE-70

EMS is proud to introduce this ready-to-use new fixative designed to offer the latest preservation of structural integrity with good immunostaining results. This fixative is a phosphate buffered ethanol (BE-70) with the addition of glycerol and glacial acetic acid.
Reference: Journal of Histochemistry & Cytochemistry 2017, Vol 65(4) p. 251-252

RT 15988   1 L 70.00 Add to Cart

arrow11Carnoy's Fixatives

Stock solution.

RT 64130-05 Carnoy's Fluid msds 500 ml 26.00 Add to Cart
RT 64130-50 Carnoy's Fluid Without Chloroform msds 500 ml 24.00 Add to Cart

msdsarrow11Carson's Phosphate Buffered Formalin

Millonig's phosphate buffered formalin as modified by Carson, Martin and Lynn, by using 4% formalin in 0.1M sodium phosphate monobasic with sodium hydroxide to pH 7.2.

RT 15732-05 Carson's Phosphate Buffered Formalin 500 ml 20.00 Add to Cart
RT 15732-10S Carson's Phosphate Buffered Formalin 1 L 33.00 Add to Cart

msdsarrow11Carson's Phosphate Buffered Paraformaldehyde

For some tissues, the use of formalin (contains 10-15% Methanol) is not suitable, we offer the alternative formaldehyde (prepared from paraformaldehyde – contains no methanol) for your convenience.

RT 15733-05 Carson's Phosphate Buffered Paraformaldehyde 500 ml 23.00 Add to Cart
RT 15733-10 Carson's Phosphate Buffered Paraformaldehyde 1 L 37.00 Add to Cart

msdsarrow11CytoPrep™ Fixative

CytoPrep Fixative forms a clear protective coating over specimens while fixing cells. It is ideal for cytological smears and it dries within 4 to 6 minutes. Good for safe storage and transportation of slides. It is water soluble and contains no chlorofluorocarbons. Comes in a 4 oz. spray can.

RT 64145-01 CytoPrep Fixative 4 oz 40.00 Add to Cart
RT 64145-12 CytoPrep Fixative 12 x 4 oz 410.00 Add to Cart

msdsCytoFixCytoFixarrow11CytoFix

Cytology fixative spray designed for slide based cytology specimens for cellular preservation and transport.

Quickly spray a fine mist over the slide (covering the entire specimen) for cellular fixation and reduce air drying of the material.

CytoFix forms a thin, tough, clear protective coating over the specimen with a water and alcohol solution of Polyethylene glycol for cellular fixation and does not require rehydration by the laboratory prior to staining. Allows safe storing or transporting of slides.

Allow specimens to dry before sending to lab or for storage.

  • Dries in 4 to 6 minutes
  • Water soluble
  • Won't cloud or obscure specimen
  • Contains no chlorofluorocarbons

Available in pump bottle or aerosol can.

22805-01 CytoFix (Non-Aerosol) 100 ml 14.50 Add to Cart
22805-02 CytoFix Can 4 oz 14.50 Add to Cart

msdsarrow11Davidson's Fixative (Hartmann's/ Davidson's Fixative)

This is rapid fixative. Results with good nuclear detail and minimal formalin pigment. Davidson's (Hartmann's) Fixative is useful for preparing tumors, bone marrow, gynecologic specimen, fatty breast, medical biopsies. Fixation of small specimen is rapid. Exposure to the fixative should be limited to 24 hours (tissue may then be transferred to neutral buffered formalin or 70% alcohol for storage).

Based on the volume of 1125 ml solution, there are 2 parts Formalin (35-39%), 3 parts Ethanol, 1 part Glacial Acetic acid, and 3 parts tap water.

RT 64133-10 Davidson's (Hartmann's) Fixative 1 L 82.00 Add to Cart
RT 64133-14 Davidson's (Hartmann's) Fixative 4 L 232.00 Add to Cart

msdsarrow11Modified Davidson's Fixative

(14% Ethyl Alcohol, Denatured, 37.5% Formalin 37-39%, 6.25% Glacial Acetic Acid, and D.I. Water).

RT 64133-50 Modified Davidson's Fixative 1 L 39.00 Add to Cart

msdsarrow11Dietrich's Fixative

Ethanol, formalin, glacial acetic acid, distilled water.

RT 15958-10 Dietrich's Fixative 10 x 10 ml 45.00 Add to Cart
RT 15958-20 Dietrich's Fixative 100 ml 44.00 Add to Cart

msdsAlcoholic Stain Solutionsarrow11Fixative/Decalcifier

  • A solution that fixes and decalcifies in one step
  • Prevents loss of cellular structure
  • Enhances hematoxylin staining
RT 64148-01 Fixative/Decalcifier 1 L 52.00 Add to Cart
RT 64148-06 Fixative/Decalcifier 6 x 1 L 310.00 Add to Cart

msdsarrow12Formalde-Fresh Low Odor Solution; Histology Grade

A specially formulated odor-mask which neutralizes the formalin odor without affecting subsequent tissue processing, handling, or staining. Low odor, ready-to-use 10% formalin solution. May be used as a fixative or preservative of specimens. Its shelf life is 24 months.

RT 15678-01 Formalde-Fresh 4 L 95.00 Add to Cart
RT 15678-04 Formalde-Fresh 4 x 4 L 364.00 Add to Cart

msdsarrow11Buffered Formalde-Fresh, Low-Odor 10% Formalin Solution

Low odor, prepared w/v, pH 6.9 to 7.1 at 25°C. 10% formalin in Phosphate buffer according to the A.F.I.P. Manual of Histology. This solution allows for long-term storage of tissue specimens.

RT 15741-01 Low-Odor Formalin 10% Buffered 4 L 65.00 Add to Cart
RT 15741-04 Low-Odor Formalin 10% Buffered 4 x 4 L 239.00 Add to Cart

msdsarrow12Formalin, Neutral, Buffered 10% w/v in Acetate Buffer

Buffered with Sodium Acetate in accordance with the A.F.I.P. manual of Histology. Filtered and ready-to-use.
pH 6.0 to ph 7.1 at 20°C.

RT 15738-01 Formalin 10% Buffered in Acetate 1 L 25.00 Add to Cart
RT 15738-04 Formalin 10% Buffered in Acetate 4 L 57.00 Add to Cart

msdsarrow12Formalin, Neutral, Buffered 10% w/v in Phosphate Buffer

Buffered with phosphate in accordance with the A.f.I.P. manual of Histology. Filtered and ready-to-use.
pH 6.9 to 7.1 at 25°C.

RT 15740 Formalin 10% Buffered in Phosphate 450 ml 18.00 Add to Cart
RT 15740-01 Formalin 10% Buffered in Phosphate 1 L 25.00 Add to Cart
RT 15740-04 Formalin 10% Buffered in Phosphate 4 L 63.00 Add to cart

msdsarrow11Formalin 1% Non Buffered

RT 15734-01 Formalin 1% Non-Buffered 1 L 22.00 Add to Cart
RT 15734-04 Formalin 1% Non-Buffered 4 L 57.00 Add to Cart

msdsarrow11Formalin 2%, Non-Buffered

RT 15735-01 Formalin 2% Non-Buffered 1 L 22.00 Add to Cart
RT 15735-04 Formalin 2% Non-Buffered 4 L 57.00 Add to Cart

msdsarrow12Formalin 3%, Non-Buffered

RT 15735-10 Formalin 3% Non-Buffered 1 L 23.00 Add to Cart
RT 15735-20 Formalin 3% Non-Buffered 4 L 58.00 Add to Cart

msdsarrow12Formalin 4%, Non-Buffered

RT 15735-30 Formalin 4% Non-Buffered 1 L 23.00 Add to Cart
RT 15735-40 Formalin 4% Non-Buffered 4 L 57.00 Add to Cart

msds arrow11Formalin 5%, Non-Buffered

RT 15735-50 Formalin 5% Non-Buffered 1 L 24.00 Add to Cart
RT 15735-60 Formalin 5% Non-Buffered 4 L 58.00 Add to Cart

msdsarrow12TissuePrep Buffered 10% Formalin

Prefilled with 10% formalin Cat# 15740. Ready-to-use in pre-filled cups. Labeled in accordance to OSHA requirements, which gives you immediate information regarding handling and first aid procedures. Pre-measured in wide-mouth, leak-proof, opaque polypropylene containers which limits your exposure to formaldehyde and saves you time.

  Catalog # Cup Size Filled Pack/cs Price  
RT 15742-10 15 ml 10 ml 50 77.00 Add to Cart
RT 15742-20 30 ml 20 ml 50 98.00 Add to Cart
RT 15742-30 60 ml 30 ml 20 44.00 Add to Cart
RT 15742-60 120 ml 60 ml 20 48.00 Add to Cart

arrow12Paraformaldehyde, Prepared Fixatives

In addition to our complete line of Formaldehyde solutions we have now added to the line.

msdsParaformaldehyde 0.5% in 0.1M phosphate buffer pH7.4
RT 15951-10 Paraformaldehyde, 0.5% each in 0.1M Phosphate Buffer 10 x 10 ml 39.00 Add to Cart
RT 15951-15 Paraformaldehyde, 0.5% each in 0.1M Phosphate Buffer 500 ml 105.00 Add to Cart
msdsParaformaldehyde 1% Aqueous
RT 15953-10 Paraformaldehyde, 1% each Aqueous 10 x 10 ml 27.00 Add to Cart
RT 15953-15 Paraformaldehyde, 1% each Aqueous 500 ml 97.00 Add to Cart
msdsParaformaldehyde 1% in 0.1M Phosphate Buffer pH, 7.4
RT 15952-10 Paraformaldehyde, 1% each in 0.1M Phosphate Buffer 10 x 10 ml 41.00 Add to Cart
RT 15952-15 Paraformaldehyde, 1% each in 0.1M Phosphate Buffer 500 ml 105.00 Add to Cart
msdsParaformaldehyde 1% Glut 2% in 0.1M Phosphate buffer, pH 7.4
RT 15949-30 Paraformaldehyde 1% Glutaraldehyde 2% in 0.1M Phosphate Buffer 10 x 10 ml 41.00 Add to Cart
RT 15949-35 Paraformaldehyde 1% Glutaraldehyde 2% in 0.1M Phosphate Buffer 500 ml 108.00 Add to Cart
msdsParaformaldehyde 3% Glutaraldehyde 0.35% in 0.1M Sodium Cacodylate, pH 7.4
RT 15949-50 Paraformaldehyde 3% Glutaraldehyde 0.35% in 0.1M Sodium Cacodylate Buffer 10 x 10 ml 44.00 Add to Cart
RT 15949-60 Paraformaldehyde 3% Glutaraldehyde 0.35% in 0.1M Sodium Cacodylate Buffer  500 ml 115.00 Add to Cart
msdsParaformaldehyde 4% and Glutaraldehyde 1% in Phosphate Buffer, pH 7.0
RT 15949-70 Paraformaldehyde 4% Glutaraldehyde 1% in 0.1M Phosphate Buffer 10 x 10 ml 48.00 Add to Cart
RT 15949-80 Paraformaldehyde 4% Glutaraldehyde 1% in 0.1M Phosphate Buffer 500 ml 115.00 Add to Cart
msdsParaformaldehyde 4% in 0.1M Sodium Cacodylate, pH7.4
RT 15952-10S Paraformaldehyde 4% in 0.1M Sodium Cacodylate Buffer 10 x 10 ml 51.00 Add to Cart
RT 15952-15S Paraformaldehyde 4% in 0.1M Sodium Cacodylate Buffer 500 ml 131.00 Add to Cart
msdsParaformaldehyde 4% in 0.1M Phosphate Buffer, pH 7.4
RT 15735-10S Paraformaldehyde 4% in 0.1M Phosphate Buffer 10 x 10 ml 39.00 Add to Cart
RT 15735-20S Paraformaldehyde 4% in 0.1M Phosphate Buffer 500 ml 109.00 Add to Cart
msdsParaformaldehyde 4% in 0.2M Phosphate Buffer, pH 7.4
RT 15735-30S Paraformaldehyde 4% in 0.2M Phosphate Buffer 10 x 10 ml 38.00 Add to Cart
RT 15735-40S Paraformaldehyde 4% in 0.2M Phosphate Buffer 500 ml 108.00 Add to Cart
msdsParaformaldehyde 4% in 0.1M Phosphate Buffer, pH 7.2
RT 15735-50S Paraformaldehyde 4% in 0.1M Phosphate Buffer 10 x 10 ml 44.00 Add to Cart
RT 15735-60S Paraformaldehyde 4% in 0.1M Phosphate Buffer 500 ml 112.00 Add to Cart
msdsParaformaldehyde 4% in 0.2M Phosphate Buffer, pH 7.2
RT 15735-70 Paraformaldehyde 4% in 0.2M Phosphate Buffer 10 x 10 ml 44.00 Add to Cart
RT 15735-80 Paraformaldehyde 4% in 0.2M Phosphate Buffer 500 ml 110.00 Add to Cart
msdsParaformaldehyde 4% in 0.1M Phosphate BufferSaline, pH7.4
RT 15735-85 Paraformaldehyde 4% in 0.1M Phosphate Buffer Saline 10 x 10 ml 45.00 Add to Cart
RT 15735-90 Paraformaldehyde 4% in 0.1M Phosphate Buffer Saline 500 ml 114.00 Add to Cart
msdsParaformaldehyde 4% in 0.2M Phosphate Buffer Saline, pH 7.4
RT 15735-95 Paraformaldehyde 4% in 0.2M Phosphate Buffer 10 x 10 ml 46.00 Add to Cart
RT 15735-100 Paraformaldehyde 4% in 0.2M Phosphate Buffer 500 ml 115.00 Add to Cart

msdsarrow12Formaldehyde, Reagent, A.C.S. (Formalin)

HCHO F.W. 30.03 CAS #50-00-0

Specific Gravity   1.09

A.C.S. Specifications

Assay   35.5 to 38.0%
Residue after ignition   0.005%
Color (A.P.H.A.)   10
Sulfate (SO4)   0.002%
Heavy Metal (as Pb)   5ppm
Titrate Acid   0.006 meq/g
Chloride (Cl)   5ppm
Iron (Fe)   5ppm
RT 15680   450 ml 16.00 Add to Cart
RT 15681   4 x 450 ml 54.00 Add to Cart
RT 15682   1 qt (946 ml) 25.00 Add to Cart

msdsarrow12Formaldehyde, 37%, Microfiltered (Formalin Microfiltered)

HCHO F.W. 30.03 CAS# 50-00-0

Microfiltered with a 0.45 micron membrane; suitable as an EM fixative when a methanol free solution is not necessary.

RT 15686   10 x 10 ml 34.00 Add to Cart

Alcoholic Stain Solutions msds arrow12Formaldehyde

(Paraformaldehyde) GLUTARALDEHYDE

Karnovsky's Fixative Kit, please click here.

RT 15720   kit 28.00 Add to Cart
RT 15730   Lot of 5 kits 126.00 Add to Cart
msdsFixative Reagent Grade (Karnovsky's)

This fixative contains 3% glutaraldehyde and 2% formaldehyde in 0.1M phosphate buffer, pH 7.4.

Karnovsky's with formalin 2% and Biological glutaraldehyde 3% in 0.1m Phosphate Buffer.

RT 15731-10 Karnovsky Fixative Reagent Grade 1 L 260.00 Add to Cart
msdsKarnovsky's with paraformaldehyde solution and EM grade glutaraldehyde

Please Note: This kit can be formulated to fit your requirements. Please contact us.

RT 15732-10 Karnovsky Fixative EM Grade 1 L 286.00 Add to Cart
Formaldehyde Solution

arrow11Formaldehyde Aqueous Solution (Paraformaldehyde Aqueous Solution) EM Grade

Paraformaldehyde – methanol free solution. A more efficient and rapid penetrant fixative used in combination with Glutaraldehyde and Acrolein fixatives. Easy to break, prescored, 10 ml ampoules sealed under inert gas, or 100 ml bottles.

CONTAINS NO PRESERVATIVES

RT 15700 16% Paraformaldehyde (formaldehyde) aqueous solution msds 10 ml 6.00 Add to Cart
RT 15710 16% Paraformaldehyde (formaldehyde) aqueous solution msds 10x10 ml 31.00 Add to Cart
RT 15710-S 16% Paraformaldehyde (formaldehyde) aqueous solution 100 ml bottle 30.00 Add to Cart
RT 15711 16% Paraformaldehyde (formaldehyde) aqueous solution 10 bxs/lot 280.00 Add to Cart
RT 15712 10% Paraformaldehyde (formaldehyde) aqueous solution msds 10x10 ml 30.00 Add to Cart
RT 15712-S 10% Paraformaldehyde (formaldehyde) aqueous solution 100 ml 29.00 Add to Cart
RT 15713 20% Paraformaldehyde (formaldehyde) aqueous solution msds 10x10 ml 34.00 Add to Cart
RT 15713-S 20% Paraformaldehyde (formaldehyde) aqueous solution 100 ml 33.00 Add to Cart
RT 15714 32% Paraformaldehyde (formaldehyde) aqueous solution msds 10x10 ml 39.00 Add to Cart
RT 15714-S 32% Paraformaldehyde (formaldehyde) aqueous solution 100 ml 38.00 Add to Cart

In addition to our complete line of Formaldehyde solutions shown above we have now added to the line.

Since the DOT (Department of Transportation) has changed the definition of what a non dangerous solution of formaldehyde is to under 10%, we are now offering an 8% and a 4% solution. This will allow us to ship overnight as well as overseas without any hazardous charges saving our customers a good deal of money.

The quality of our 8% and 4% aqueous Paraformaldehyde solution is as good as all of the other concentrations that we offer.

CONTAINS NO PRESERVATIVES

RT 157-4 4% Paraformaldehyde (formaldehyde) aqueous solution msds 10 x 10 ml 27.00 Add to Cart
RT 157-4-100 4% Paraformaldehyde (formaldehyde) aqueous solution 100 ml 26.00 Add to Cart
RT 157-8 8% Paraformaldehyde (formaldehyde) aqueous solution msds 10 x 10 ml 26.00 Add to Cart
RT 157-8-100 8% Paraformaldehyde (formaldehyde) aqueous solution 100 ml 25.00 Add to Cart

msdsaarrow11Formaldehyde/Zinc Fixative

Prepared: Ready-to-use.

Features

  • Preserves fine nuclear details
  • Good for immunostaining
  • Contains no mercury
  • Can be used with automated tissue processors
  • Works well with standard and special stains because zinc is a stain enhancing mordant
RT 15675-01 Formaldehyde/Zinc Fixative 1 L 21.00 Add to Cart
RT 15675-04 Formaldehyde/Zinc Fixative 4 L 69.00 Add to Cart

msdsarrow12Glyoxal 3% Fixative, Ready to Use

An alternative fixative to Formalin and Formaldehyde

EMS is proud to introduce an alternative to Formalin and Formaldehyde in many different applications.

Our unique kit comes premixed and measured and ready to use. The kit consists of 2 vials: Solution A & Solution B (Absolute Ethanol, Glyoxal Solution, Acetic Acid and water).

Instructions

Add solution A to solution B. Vortex the two solutions together and bring to a pH of 4.5 with the addition of NaOH 1M. The solution needs to be used immediately once mixed.

References

Sabatini DD. et al (1963) "The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation". J. Cell Biology. 17:19-58

Janigan DT. et al (1965) "The effects of aldehyde fixation on acid phosphatase activity in tissue blocks". J. Histochemistry. Cytochemistry. 13: 476-483

Dapson RW. et al (2006) "Glyoxal fixation and its relationship to immunocytochemistry." J. Histotechnology. 29: 65-76

Dapson RW. Et al (2007) "Glyoxal fixation: how it works and why it only occasionally needs antigen retrieval" lnforma healthcare, Dapson & Dapson, LLC

Richter K. et al (2018) "Glyoxal as an alternative to formaldehyde in immunostaining and super-resolution microscopy" The EMBO Journal. 37: 139-159

2-8°C 16525 Glyoxal 3% Fixative 100 ml 52.00 Add to Cart

arrow11Ampoules Breakers and Crackers

Please see the Sample Preparation section.

arrow12Perfusion Wash and Fixative Ready to Use

For all of the best results we offer ready to use Perfusion washes and fixatives which are recommended by the NeuroScience Association. All of our solutions are ready made and are of the highest quality.

We have three groups of solutions commercially available.

Group I

Perfusion wash and Fixative Technical Grade. To be used for Standard stains.

Perfusion Wash (1220SK): Phosphate Buffered Saline 01.M
Phosphate Buffer pH 7.2-7.4

Perfusion Fixative(1221SK): 10% Phosphate Buffered
Formaldehyde.

Group II

Perfusion wash and Fixative Reagent Grade. To be used for Standard and ImmunoHistochemistry Stains.

Perfusion Wash (1219SK): Phosphate Buffered Saline 0.1M
Phosphate Buffer pH 7.2-7.4.

Perfusion Fixative (1224SK): Paraformaldehye 4%/Sucrose
4% in Phosphate Buffer 0.1M Ph 7.2.

Group III

Perfusion wash and Fixative Superior Reagent Grade.

For the best results use this group. This is Sodium cacodylate based for NSA'S Disintegrative Degeneration Stain.

Perfusion Wash (1222SK): 0.8% NaCl/0.4% Dextrose/0.8% Sucrose/ 0.023% CaCl2 /Sodium.

Cacodylate 0.034%.

Perfusion Fixative (1223SK): 4% Paraformaldehyde/4% Sucrose/ 1.4% Sodium Cacodylate.

For a complete procedure and protocol for the use of these washes and fixatives please see the following site: www.neuroscienceassociates.com/perf-solution1 or 2 or 3.htm.

  1220SK Perfusion Wash, Technical msds 1000 ml 34.00 Add to Cart
  1221SK Perfusion Fixative, Technical msds 1000 ml 48.00 Add to Cart
  1219SK Perfusion Wash, Reagent msds 1000 ml 32.00 Add to Cart
  1224SK Perfusion Fixative, Reagent msds 1000 ml 49.00 Add to Cart
  1222SK Perfusion Wash, Super Reagent msds 1000 ml 35.00 Add to Cart
  1223SK Perfusion Fixative, Super Reagent msds 1000 ml 52.00 Add to Cart

If you want a custom wash or fixative made please just let us know and we will be happy to make you whatever you require.

Technical Tip:

The Fixative for Use in Fixing Cornea

arrow11CoreDish™ Multiple Biopsy Containers Half Prefilled with 10% Formalin

Few recommendations concerning how the biopsies should be handled have been published. Performing a large number of biopsies means an increase in the number of containers handled and consequently a technical overload of the transmission network, which occurs without any financial counterpart. A new approach had to be developed in order to increase productivity.

We are proud to offer a multi-compartment container in the shape of a dish and half prefilled with 10% Neutral buffered Formalin, for holding and transporting biopsies. It is supplied with a leak-proof closure with O-ring ensuring total protection of contents. It conforms to OSHA directives. These CoreDisn™ measure only 15mm H x 95 mm Diameter. Each compartment is clearly identified to allow proper placement and visualization of the biopsy being inserted. Thanks to CoreDish™ it is no more necessary to use a multiple of individual containers, thereby reducing cost and risks of confusion.

CoreDish™ available in many configurations to serve in different biopsy, such as prostate, upper GI tract and lower GI tract. A label allows recording the related information, patient ID, doctor, date and time.

CoreDish™ Breast Biopsy Containerarrow11M970-D5B-2 CoreDish™ Breast Biopsy Container

Half Prefilled with 10% Formalin

This multi-compartment container (out of five compartments, four are labeled: LUQ, RUQ, LLQ, RLQ) in the shape of a dish and half prefilled with 10% Neutral Buffered Formalin, for holding and transporting biopsies. It is supplied with a leak-proof screw closure with O-ring ensuring total protection of contents. It conforms to OSHA directives. The dish measures only 15 x 95 mm in diameter. Each compartment is clearly identified to allow proper placement and visualization of the breast biopsy being inserted. A writing area for patient information is provided.

RT 15736-10 Breast, 5 compartments 10/pk 64.00 Add to Cart
CoreDish™ Prostate Biopsy Container arrow12M970-D8P CoreDish™ Prostate Biopsy Container

Half Prefilled with 10% Formalin

For prostate biopsies. Eight compratments. Leakproof seal with O-ring lid. An area for patient information is provided. Seven labeled compartments: Base, Lateral Base, Medium, Lateral Medium, Apex, Lateral Apex.

RT 15736-20 Prostate, 8 Compartments 10/pk 64.00 Add to Cart
CoreDish™ Upper GI Biopsy Container arrow12M970-D8UGI CoreDish™ Upper GI Biopsy Container

Half Prefilled with 10% Formalin

For upper GI track biopsies. Eight compratments. Leakproof seal with O-ring lid. An area for patient information is provided. Seven labeled compartments: Gastric Card, Gastric Body, GE Junction, Gastric ATR, Distal Esophage, Pylorus, Duodeum.

RT 15736-30 Upper GI, 8 Compartments 10/pk 64.00 Add to Cart
CoreDish™ Lower Gi Biopsy Container arrow11M970-D8LGI CoreDish™ Lower Gi Biopsy Container

Half Prefilled with 10% Formalin

For lower GI track biopsies. Twelve compratments. Leakproof seal with O-ring lid. An area for patient information is provided. Ten labeled compartments: Proximal Flexure Colon, Hepatic Fleure Colon, Distal Transverse Colon, Ascending Colon, Splenic flexure Colon, Cecum, Decending Colon, Terminal lleum, Rectum, Sigmmoid Colon.

RT 15736-40 Lower GI, 12 Compartments 10/pk 64.00 Add to Cart
CoreDish™ Prostate Biopsy Container arrow11M970-D12P CoreDish™ Prostate Biopsy Container

Half Prefilled with 10% Formalin

For prostate biopsies. Twelve compratments. Leakproof seal with O-ring lid. An area for patient information is provided. Twelve labeled compartments: L Base, R Base, L Lateral Base, R Lateral Base, L Medium, R Medium, L Lateral Medium, R Lateral Medium, L Lateral, L Apex, R Apex, R Lateral Apex.

RT 15736-50 Prostate, 12 Compartments 10/pk 64.00 Add to Cart

arrow11Glutaraldehyde

OCHCH2CH2CH2CHO F.W. 100.12 CAS #111-30-8
Specific Gravity @20/20°C: 1.131

EM Grade Distillation Purified

  • The Universal Fixative for Electron Microscopy
  • Purified to EM Grade from commercial grade by "all-glass vacuum-distillation"
  • THE HIGHEST PURITY, free from polymers and other contaminants
  • Each lot is tested and assayed in our lab before we ship and guarantee its purity
  • Sealed under dry nitrogen
  • Refrigerated prior to shipment

After glutaraldehyde has been purified to EM grade, it has a high risk of polymerizing if it is not handled properly. Electron Microscopy Sciences takes special precautions to assure a polymer-free, pure glutaraldehyde.

Every lot of our EM grade aqueous glutaraldehyde is assayed prior to shipping assuring you consistent purity and the highest possible quality. If stored properly, our Glutaraldehyde has a very long shelf life.

We package our aqueous glutaraldehyde in 10 ml, single dose ampoules, sealed under dry nitrogen, and kept refrigerated until it is shipped. We offer our glutaraldehyde in a wide range of percentages to simplify mixing procedures and avoiding waste.

EM grade aqueous glutaraldehyde, stored in volumes greater than 100 ml, with repeated defrosting and recapping, will polymerize in time, producing an absorption peak of 235nm rather than the 280nm of pure EM grade. The presence of polymerized glutaraldehyde greatly reduces its efficiency as a cross-linking agent, preventing proper fixation. For this reason, we provide 10 ml ampoules and 100 ml bottle quantities for larger applications.

msds8% Aqueous Solution
4 to (-18)°C 16000   10 ml ampoule 6.00 Add to Cart
4 to (-18)°C 16019   Box 10 x 2ml 23.00 Add to Cart
4 to (-18)°C 16020   Box 10 x 10 ml ampoules 27.00 Add to Cart
4 to (-18)°C 16021 same as 16020 10 bxs 260.00 Add to Cart
4 to (-18)°C 16022 same as 16020 20 bxs 445.00 Add to Cart
4 to (-18)°C 16010   100 ml bottle 26.00 Add to Cart
msds10% Aqueous Solution
4 to (-18)°C 16100   10 ml ampoule 6.00 Add to Cart
4 to (-18)°C 16120   Box 10 x 10 ml ampoules 30.00 Add to Cart
4 to (-18)°C 16121 same as 16120 10 bxs 278.00 Add to Cart
4 to (-18)°C 16122 same as 16120 20 bxs 475.00 Add to Cart
4 to (-18)°C 16110   100 ml bottle 26.00 Add to Cart
msds25% Aqueous Solution
4 to (-18)°C 16200   10 ml ampoule 7.00 Add to Cart
4 to (-18)°C 16220   Box 10 x 10 ml ampoules 33.00 Add to Cart
4 to (-18)°C 16221 same as 16220 10 bxs 315.00 Add to Cart
4 to (-18)°C 16222 same as 16220 20 bxs 550.00 Add to Cart
4 to (-18)°C 16210   100 ml bottle 26.00 Add to Cart
msds50% Aqueous Solution
4 to (-18)°C 16300   10 ml ampoule 7.50 Add to Cart
4 to (-18)°C 16320   Box 10 x 10 ml ampoules 45.00 Add to Cart
4 to (-18)°C 16321 same as 16320 10 bxs 430.00 Add to Cart
4 to (-18)°C 16322 same as 16320 20 bxs 780.00 Add to Cart
4 to (-18)°C 16310   100 ml bottle 44.00 Add to Cart
msds70% Aqueous Solution
4 to (-18)°C 16350   10 ml ampoule 8.50 Add to Cart
4 to (-18)°C 16360   box 10 x 10 ml ampoules 62.00 Add to Cart
4 to (-18)°C 16365   box 10 x 2ml ampoules 37.00 Add to Cart
msdsmsdsBiological Grade
4 to (-18)°C 16400 25% Solution 450 ml 20.00 Add to Cart
4 to (-18)°C 16401 25% Solution 4 x 450 ml 76.00 Add to Cart
4 to (-18)°C 16410 25% Solution 1 gal. 74.00 Add to Cart
4 to (-18)°C 16411 25% Solution 4 x 1 gal. 269.00 Add to Cart
4 to (-18)°C 16500 50% Solution 450 ml 23.00 Add to Cart
4 to (-18)°C 16501 50% Solution 4 x 450 ml 80.00 Add to Cart
4 to (-18)°C 16520 50% Solution 1 liter 43.00 Add to Cart
4 to (-18)°C 16521 50% Solution 4 x 1 liter 154.00 Add to Cart

msdsmsdsarrow11Serum Vial Aqueous Glutaraldehyde

Over the years, many of our valued customers have requested purchasing our aqueous Glutaraldehyde in serum vials. This packaging allows the user to puncture the special non-reactive seal with a hypodermic syringe and draw out the exact quantity of aqueous Glutaraldehyde needed without opening the container. This packaging assures freshness and purity of the aqueous Glutaraldehyde at all times.

4 to (-18)°C 16216 25% EM Grade Aqueous Glutaraldehyde in Serum Vial 10 x10 ml 46.00 Add to Cart
4 to (-18)°C 16214 25% EM Grade Aqueous Glutaraldehyde in Serum Vial 100 ml 38.00 Add to Cart
4 to (-18)°C 16316 50% EM Grade Aqueous Glutaraldehyde in Serum Vial 10 x10 ml 58.00 Add to Cart
4 to (-18)°C 16314 50% EM Grade Aqueous Glutaraldehyde in Serum Vial 100 ml 47.00 Add to Cart

* All other concentrations are available in serum vials upon request.

For any special concentration, or packaging requirements other than those already listed, please call us today. We will manufacture in accordance to your specifications.

arrow11Anhydrous EM Grade Glutaraldehyde: Specially Prepared-10% Solution

Rapid-Freezing on a polished metal surface, at the temp. of liquid helium or liquid nitrogen, followed by freeze-substitution in osmium-acetone, has been shown to be a very useful technique for capturing rapid cellular events and for preserving cell ultrastructure.
* John M. Robinson and Morris J. Karnovsky, 1991. "Rapid-freezing Cytochemistry: Preservation of Tubular Lysosomes and Enzyme Activity". J. of Histochem., Cytochem. Vol. 39, No. 6, pp 787-792.

Ordering Information: (please call for additional information)

0-5°C 16530 10% in Acetone msds 10 x 10 ml 88.00 Add to Cart
0-5°C 16531 10% in Ethanol msds 10 x 10 ml 88.00 Add to Cart
0-5°C 16532 10% in Methanol msds 10 x 10 ml 88.00 Add to Cart
0-5°C 16533 10% in Solvent of your choice   10 x 10 ml 88.00 Add to Cart

We would be pleased to fill your specific glutaraldehyde needs. Please call for prices on special concentrations or size requirements.

arrow11Glutaraldehyde, Pre-Made, Ready-to-Use, in Buffer

msdsGlutaraldehyde 0.625% in 0.1M Phosphate Buffer, pH 7.4
0-5°C 16535-05 Glutaraldehyde 0.625% in Phosphate Buffer, pH 7.4 500 ml 144.00 Add to Cart
0-5°C 16535-10 Glutaraldehyde 0.625% in Phosphate Buffer, pH 7.4 1 L 272.00 Add to Cart
msdsGlutaraldehyde 2% in 0.1M Phosphate Buffer, pH 7.4
0-5°C 16536-05 Glutaraldehyde 2% in Phosphate Buffer, pH 7.4 500 ml 144.00 Add to Cart
0-5°C 16536-10 Glutaraldehyde 2% in Phosphate Buffer, pH 7.4 1 L 280.00 Add to Cart
msdsGlutaraldehyde 2% in 0.1M Sodium Cacodylate Buffer, pH 7.4
0-5°C 16536-15 Glutaraldehyde 2% in Sodium Cacodylate Buffer, pH 7.4 500 ml 155.00 Add to Cart
0-5°C 16536-20 Glutaraldehyde 2% in Sodium Cacodylate Buffer, pH 7.4 1 L 292.00 Add to Cart
msdsGlutaraldehyde 2.5% in 0.1M Phosphate Buffer, pH 7.4
0-5°C 16537-05 Glutaraldehyde 2.5% in Phosphate Buffer, pH 7.4 500 ml 162.00 Add to Cart
0-5°C 16537-10 Glutaraldehyde 2.5% in Phosphate Buffer, pH 7.4 1 L 295.00 Add to Cart
msdsGlutaraldehyde 2.5% in 0.1M Sodium Cacodylate Buffer, pH 7.4
0-5°C 16537-15 Glutaraldehyde 2.5% in Sodium Cacodylate Buffer, pH 7.4 500 ml 161.00 Add to Cart
0-5°C 16537-20 Glutaraldehyde 2.5% in Sodium Cacodylate Buffer, pH 7.4 1 L 300.00 Add to Cart
msdsGlutaraldehyde 3% in 0.1M Phosphate Buffer, pH 7.4
0-5°C 16538-05 Glutaraldehyde 3% in Phosphate Buffer, pH 7.4 500 ml 166.00 Add to Cart
0-5°C 16538-10 Glutaraldehyde 3% in Phosphate Buffer, pH 7.4 1 L 300.00 Add to Cart
msdsGlutaraldehyde 3% in 0.1M Sodium Cacodylate Buffer, pH 7.4
0-5°C 16538-15 Glutaraldehyde 3% in Sodium Cacodylate Buffer, pH 7.4 500 ml 168.00 Add to Cart
0-5°C 16538-20 Glutaraldehyde 3% in Sodium Cacodylate Buffer, pH 7.4 1 L 305.00 Add to Cart
msdsGlutaraldehyde 3% Sodium Phosphate Buffer, pH 7.2
0-5°C 16539-45 Glutaraldehyde 3% in Sodium Phosphate Buffer, pH 7.2 10 x 10 ml 39.00 Add to Cart
0-5°C 16539-50 Glutaraldehyde 3% in Sodium Phosphate Buffer, pH 7.2 500 ml 139.00 Add to Cart
0-5°C 16539-55 Glutaraldehyde 3% in Sodium Phosphate Buffer, pH 7.2 1 L 282.00 Add to Cart
msdsGlutaraldehyde 4% in 0.1m Phosphate Buffer, pH 7.3
0-5°C 16539-60 Glutaraldehyde 4% in 0.1m Phosphate Buffer, pH 7.3 10 x 10 ml 41.00 Add to Cart
0-5°C 16539-65 Glutaraldehyde 4% in 0.1m Phosphate Buffer, pH 7.3 500 ml 139.00 Add to Cart
0-5°C 16539-70 Glutaraldehyde 4% in 0.1m Phosphate Buffer, pH 7.3 1 L 278.00 Add to Cart
msdsGlutaraldehyde 2% Paraformaldehyde 2% in Phosphate Buffer, pH 7.4
0-5°C 16536-06 Glutaraldehyde 2% Paraformaldehyde 2% in Phosphate Buffer, pH 7.4 100 ml 40.00 Add to Cart
0-5°C 16536-07 Glutaraldehyde 2% Paraformaldehyde 2% in Phosphate Buffer, pH 7.4 500 ml 159.00 Add to Cart
0-5°C 16536-08 Glutaraldehyde 2% Paraformaldehyde 2% in Phosphate Buffer, pH 7.4 1 Liter 286.00 Add to Cart
msdsGlutaraldehyde 2.5% Paraformaldehyde 1% in Phosphate Buffer, pH 7.4
0-5°C 16537-06 Glutaraldehyde 2.5% Paraformaldehyde 1% in Phosphate Buffer, pH 7.4 100 ml 38.00 Add to Cart
0-5°C 16537-07 Glutaraldehyde 2.5% Paraformaldehyde 1% in Phosphate Buffer, pH 7.4 500 ml 158.00 Add to Cart
0-5°C 16537-08 Glutaraldehyde 2.5% Paraformaldehyde 1% in Phosphate Buffer, pH 7.4 1 Liter 284.00 Add to Cart
msdsGlutaraldehyde 2.5% Aqueous
0-5°C 16537-16 Glutaraldehyde 2.5% Aqueous 10 x 10 ml 26.00 Add to Cart
0-5°C 16537-17 Glutaraldehyde 2.5% Aqueous 500 ml 68.00 Add to Cart
0-5°C 16537-18 Glutaraldehyde 2.5% Aqueous 1 Liter 118.00 Add to Cart
msdsGlutaraldehyde 3.0% Aqueous
0-5°C 16537-19 Glutaraldehyde 3% Aqueous 100 ml 28.00 Add to Cart
0-5°C 16537-20-S Glutaraldehyde 3% Aqueous 500 ml 68.00 Add to Cart
0-5°C 16537-21 Glutaraldehyde 3% Aqueous 1 Liter 118.00 Add to Cart
msdsGlutaraldehyde 3.0% Paraformaldehyde 3% in 0.1M Phosphate Buffer, pH 7.4
0-5°C 16538-06 Glutaraldehyde 3% Paraformaldehyde 3% in Phosphate Buffer, pH 7.4 10 x 10 ml 28.00 Add to Cart
0-5°C 16538-07 Glutaraldehyde 3% Paraformaldehyde 3% in Phosphate Buffer, pH 7.4 500 ml 68.00 Add to Cart
0-5°C 16538-08 Glutaraldehyde 3% Paraformaldehyde 3% in Phosphate Buffer, pH 7.4 1 Liter 98.00 Add to Cart
msdsGlutaraldehyde 4% in 0.1M in Sodium Cacodylate Buffer, pH 7.4
0-5°C 16539-06 Glutaraldehyde 4% in 0.1M Sodium Cacodylate Buffer, pH 7.4 10 x 10 ml 40.00 Add to Cart
0-5°C 16539-07 Glutaraldehyde 4% in 0.1M Sodium Cacodylate Buffer, pH 7.4 500 ml 140.00 Add to Cart
0-5°C 16539-08 Glutaraldehyde 4% in 0.1M Sodium Cacodylate Buffer, pH 7.4 1 Liter 280.00 Add to Cart
msdsGlutaraldehyde 5% Aqueous
0-5°C 16539-15 Glutaraldehyde 5% Aqueous 10 x 10 ml 27.00 Add to Cart
0-5°C 16539-20 Glutaraldehyde 5% Aqueous 500 ml 68.00 Add to Cart
0-5°C 16539-25 Glutaraldehyde 5% Aqueous 1 Liter 118.00 Add to Cart
msdsGlutaraldehyde 5% in 0.1M Phosphate Buffer, pH 7.4
0-5°C 16539-30 Glutaraldehyde 5% in 0.1M Phosphate Buffer, pH 7.4 10 x 10 ml 40.00 Add to Cart
0-5°C 16539-35 Glutaraldehyde 5% in 0.1M Phosphate Buffer, pH 7.4 500 ml 139.00 Add to Cart
0-5°C 16539-40 Glutaraldehyde 5% in 0.1M Phosphate Buffer, pH 7.4 1 Liter 279.00 Add to Cart

arrow11Glutaraldehyde/Formaldehyde

msdsFixative (Karnovsky's)
This fixative contains 3% glutaraldehyde and 2% formaldehyde in 0.1M phosphate buffer, pH 7.4.

Karnovsky's with formalin 2% and Biological glutaraldehyde 3% in 0.1m Phosphate Buffer.

0-5°C 15731-10 Karnovsky Fixative Reagent Grade 1 L 260.00 Add to Cart

msdsKarnovsky's with paraformaldehyde solution and EM grade glutaraldehyde.

Please Note: This kit can be formulated to fit your requirements. Please contact us.

RT 15732-10 Karnovsky Fixative EM Grade 1 L 286.00 Add to Cart
Ampoule Breaker; Soft Silicon

arrow12Ampoule Breaker; Soft Silicone

Reusable – Made from soft silicone. It offers a simple way to break pre-scored glass ampoules safely. Accommodates 5, 7, and 10 ml size ampoules. Reduces injury and spillage.

60600 Ampoule Breaker each 16.00 Add to Cart

Ampoule Crackerarrow11Ampoule Cracker™

The Ampoule Cracker™ is a disposable plastic fracture and safety collar, which is specially designed to aid in the breaking open of pre-scored glass ampoules. Simply slip the Ampoule Cracker™ over the top at the pre-scored portion, snap it, and discard both the ampoule top and the safety collar to prevent cross contamination.

60605-04 Ampoule Cracker for Ampoule Size 1-4 ml 500/pk 41.00 Add to Cart
60605-10 Ampoule Cracker for Ampoule Size 5-10 ml 500/pk 41.00 Add to Cart
60605-20 Ampoule Cracker for Ampoule Size 10-20 ml 500/pk 77.00 Add to Cart

Break-Safe Ampoule OpenerBreak-Safe Ampoule opener

arrow12Break-Safe™ Ampoule Opener

EMS Introduces Break-Safe™ allowing you to safely open up to 3 ampoules at one time.

Box style design allows one handed opening of up to 3 ampoules at a time. Gently pressing down on the lid snaps ampoules necks clean while keeping them contained within the device and away from fingers. After removing ampoules contents, simply turn the device upside down over a trash receptacle to safely discard ampoules and tops.

Features

  • Includes 4 inserts to allow opening of 1ml, 2ml, 5ml and 10 ml ampoules.
  • Open ampoules easily and safely
  • Transparency allows unobstructed viewing of ampoules contents at all times.
  • Copolyester plastic construction has broad chemical resistance
60607 Break-Safe™ Ampoule Opener each 50.00 Add to Cart

msdsarrow11HISTOCHOICE™MB®* – The Molecular Biology Fixative

  • SAFE – Contains no Formaldehyde, Glutaraldehyde or Mercury
  • NON-TOXIC – Is completely odorless and can be safely disposed
    of down the drain

HISTOCHOICE™ MB® Tissue Fixative is the first fixative designed for the molecular biologist. It is specialty formulated to preserve antigenic sites for antibody probes and nucleic acid sites for immunohistochemistry. HISTOCHOICE™MB® replaces formaldehyde based, alcohol based, Zenkers, B-5, B-3, Bouin's and other fixatives with superior results. Tissue fixed in HISTOCHOICE™MB® exhibit vibrant staining, better nuclear and cytoplasmic detail, and will retain a crisp appearance even after long-term fixation.

Because this formula is designed specially for molecular biology applications, HISTOCHOICE™MB® fixed sections do not require pre-digestion or other recovery procedures to make important sites available.This means you spend less time preparing slides and more time doing research.

More than a fixative, HISTOCHOICE™MB® is a preservative that leaves antigens and nucleic acids in their native state, allowing binding for specific probes. Primary antibodies can often be diluted several-fold due to the increased number of preserved antigenic sites, saving cost on every slide you process.

HISTOCHOICE™MB® fixed tissue retains a much more natural look and feel than formalin fixed tissue. It is important to remember that these tissues are not fixed by formalin, but rather preserved by HISTOCHOICE™MB®.

HISTOCHOICE™MB® is on long-term backorder... please check back for updates.

RT 64115-01 HISTOCHOICE™MB® Fixative 1 L 60.00 Add to Cart
RT 64115-04 HISTOCHOICE™MB® Fixative 4 L 190.00 Add to Cart
RT 64115-44 HISTOCHOICE™MB® Fixative 4 x 4 L 695.00 Add to Cart

* HISTOCHOICE is registered name of Amresco

msdsarrow11Hollande's Fixative

A modification of Bouin's solution. It is stable and will decalcify small bone specimens. Tissue that is fixed with Hollande's can be stained successfully with most stains, and the cupric acetate in the solution stabilizes red blood cell membranes and cosinophil and endocrine cell granules so that less lysis occurs than with Bouin's solution. Hollande's is widely used as a fixative for biopsy specimens of the gastrointestinal tract.

Thorough washing of the fixative prior to placing the specimen in a phosphate buffered formalin solution is necessary because the salts present in the solution will form an insoluble phosphate precipitate. Packaged in leak-proof histo-containers; prefilled ready-to-use.

  Catalog # Cup Size Filled Pack/cs Price  
RT 64120-10 15 ml 10 ml 50 78.00 Add to Cart
RT 64120-20 30 ml 20 ml 50 98.00 Add to Cart
RT 64120-30 60 ml 30 ml 20 46.00 Add to Cart

Bulk packaging is available

RT 64120-01 Hollande's Fixative 1 L 46.00 Add to Cart
RT 64120-04 Hollande's Fixative 4 L 168.00 Add to Cart

msdsarrow11McDowell Trumps Fixative

RT 18030-05 McDowell Trumps Fixative 500 ml 33.00 Add to Cart
RT 18030-10 McDowell Trumps Fixative 1 L 39.00 Add to Cart

msdsarrow11Michel's Fixative, pH 7.0-7.4

RT 18040-05 Michel's Fixative 500 ml 50.00 Add to Cart
RT 18040-10 Michel's Fixative 1 L 85.00 Add to Cart
RT 18040-20 Michel's Fixative Pre-filled 20 x 10 ml 75.00 Add to Cart

Technical Tip:

Preparation of Tissue Biopsy in Michel's Fixative.

arrow12Michel's Transport Medium

Michel's Transport Medium allows for the shipping of specimens for further immunofluorescence work. It preserves morphology and antigenicity during transport. It is not a buffer or fixative. It is simply a vehicle to transport samples. The solution must be stored at refrigerated temperatures. Do not freeze. Samples may be stored up to 5 days in this medium at room temperature.

Features

  • Comes in 250ml, 500ml, 1 Liter, Gallon, or 15ml fill in SecurTainer™
  • Can be used with Michel's Buffer (washing solution)
4-8°C 18042-05 Michel's Transport Medium 250 ml 28.00 Add to Cart
4-8°C 18042-10 Michel's Transport Medium 500 ml 48.00 Add to Cart
4-8°C 18042-15 Michel's Transport Medium 1 L 65.00 Add to Cart
4-8°C 18042-16 Michel's Transport Medium gal 142.00 Add to Cart
4-8°C 18042-17 Michel's Transport Medium, 15ml fill in SecurTainer™ 50/cs 80.00 Add to Cart

15560arrow11Mirsky's Fixative™

  • Saponin/Glyoxylate Fixative
  • Preserves Immunohistological activity
  • Contains no formaldehyde
  • Fast-acting

Mirsky's Fixative™ is a superior fixing agent for use in immunohistological and immunological protocols. Mirsky's contains no formaldehyde or glutaraldehyde. Therefore, it has considerably reduced toxicity and virtually no odor. Mirsky's Fixative is neutral, buffered, and isotonic (308 mOsmol). Additionally, Mirsky's Fixative does not contain toxic or hazardous buffers such as cacodylate or barbital. Hardening or shrinkage of tissue is considerably reduced.

Double reactive sites afford excellent cross-linking properties while maintaining sample enzyme activity. Samples processed in Mirsky's Fixative for light microscopy can subsequently be used in electron microscopy procedures. Special buffer systems may be used in place of the buffer provided.
Use as replacement for formalin and/or glutaraldehyde fixatives in immunohistological and immnocytological staining protocols. Tissue size is unlimited, as long as the sample is no thicker than 0.5cm in at least one plane to assure uniformity of tissue penetration. The gross visual appearance will be different than in formaldehyde and tissue may seem "raw". This is due to double site binding of the fixative with a resultant reduction in tissue shrinkage. Microscopic examination of tissue morphology will be noticeably improved. This material is intended to maintain enzyme and antibody activity. For best results, tissue section of high digestive enzyme content should be thoroughly rinsed in saline solution before fixation (e.g. trypsin in intestinal samples). Once fixed, tissue may be retained in Mirsky's Fixative and ethanol indefinitely.

Mirsky's Fixative is normally distributed as a concentrated two bottle system, although high through-put laboratories often use the single bottle ready-to-use format. The two bottle system is comprised of Mirsky's Fixative 10X Concentrated and Mirsky's Fixative 10X Buffer. To reconstitute to working strength, add 1 part Mirsky's Fixative 10X Buffer to 8 parts distilled or deionized water, mix thoroughly, then add 1 part Mirsky's Fixative 10X Concentrate and mix again.

The two bottle system has a shelf life of 12 months while the ready-to-use format has a shel life of 30 days.

RT 15560 Mirsky's Fixative System msds
msds		 200 ml 42.00 Add to Cart
RT 15561-05 Mirsky's Fixative Ready-to-Use in Ethanol msds 500 ml 24.00 Add to Cart
RT 15561-10 Mirsky's Fixative Ready-to-Use in Methanol 1L 42.00 Add to Cart
RT 15561-20 Mirsky's Fixative Ready-to-Use in Solvent of your choice 2L 82.00 Add to Cart

msdsarrow11Russell's Zenker Fixative (Russel's Modified Zenker)

Due to the hazardous nature of mercury, Zenker's fluid is no longer available. In place we are offering Russell's Zenker Fixative, which contains Zinc Chloride. The staining results produced by this fixative are comparable to those of Zenker's Stock solution. Russell's Modified Zenker's is used in the same manner as Zenker's stock solution.

RT 64123-05 Russell's Zenker Fixative 500 ml 27.00 Add to Cart
RT 64123-06 Russell's Zenker Fixative 1 L 46.00 Add to Cart

msdsarrow11Formic Acid 96%, Reagent, A.C.S.

HCOOH   F.W. 46.03   CAS #64-18-6
Specific Gravity: 1.22

RT 15750 Formic Acid, 96% 225 ml 19.00 Add to Cart
RT 15760 Formic Acid, 96% 1 liter 50.00 Add to Cart

msdsarrow11Gold Chloride, Hydrate(Chloroauric Acid; Hydrogen Tetrachloroaurate)

H(AuCl4)·H2O   F.W. 357.79   CAS #16961-25-4
Assay 99.9985%
Gold contents 49%+
A highly purified gold compound for the preparation of Colloidal Gold sols. Preweighed to facilitate your work. (Preparation instructions for Gold Sols included).

RT 16580   10 x 1/10g 156.00 Add to Cart
RT 16582   10 x ½g 625.00 Add to Cart
RT 16583   1g 140.00 Add to Cart
RT 16584   10x1g 1,195.00 Add to Cart

arrow11Gold Chloride Aqueous Solutions

RT 16586 Gold Chloride, 0.1% Aqueous msds 500 ml 72.00 Add to Cart
RT 16587 Gold Chloride, 0.2% Aqueous msds 500 ml 112.00 Add to Cart
RT 16588 Gold Chloride, 0.5% Aqueous msds 500 ml 226.00 Add to Cart
RT 16589 Gold Chloride, 1.0% Aqueous msds 500 ml 506.00 Add to Cart
RT 16590 Gold Chloride, 10.0% Aqueous msds 500 ml 3,995.00 Add to Cart

arrow11Gold, Immuno, see Section 2 ImmunoGold Reagents

arrow12Gum Mastic

0-4°C 16590-01 Gum Mastic, 2.5% in Alcohol msds 500 ml 111.00 Add to Cart
0-4°C 16591 Gum Mastic, 5% in Alcohol msds 500 ml 116.00 Add to Cart
0-4°C 16592 Gum Mastic, 10% in Alcohol msds 500 ml 143.00 Add to Cart
0-4°C 16595 Gum Mastic, 10% in Isopropanol 500 ml 143.00 Add to Cart

msdsmsdsarrow12Hexamethylenetetramine, A.C.S. Reagent, Granular

(Urotropine; Methenamine)

C6N4   F.W. 140.19   CAS #100-97-0

Assay:   99.0-100.5%

Specifications

Residue after Ignition   0.1%
Chloride   0.014%
Sulfate   To Pass Test
Heavy Metals.   0.001%

Used in silver stains.

Swift, J.A. (1968) Microsc. Soc. 88, 449.

RT 16710   50g 14.00 Add to Cart
RT 16710-25*   25g 8.50 Add to Cart
RT 16712 Methynamine, 3% Aqueous 500 ml 25.00 Add to Cart

msdsarrow12Hexylene Glycol, Reagent, A.C.S.

(2-Methyl-2,4-pentanediol)

(CH3)2C(OH)CH2CH(OH)CH3   F.W. 118.18   CAS #107-41-5

Specific Gravity @25°C 0.923

A.C.S. Specifications

Assay   99%
IR Spectrum   To Pass Test
Refractive Index @20°C   0.921-0.923

Mixed with water in all proportions. Used as a dehydrating agent for fat tissues.

RT 16740   450 ml 27.00 Add to Cart
RT 16750   4 x 450 ml 99.00 Add to Cart

Reagents: HISTOCHOICE to Zinc/Formalin arrow13arrow13

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Electron Microscopy Sciences specializes in the manufacturing, preparation and distribution of the highest quality laboratory chemicals and microscopy supplies and equipment for electron microscopy, light microscopy, and histology.