Reagents for Histology: Fixing, Clearing, and Dehydrating
EMS is now offering a complete line of reagents for Histopathology:The highest quality for Fixing, Dehydrating/Clearing and Staining.
Acetate Buffer
| RT | 11482-42 | Acetate Buffer, 0.2M, pH 4.2 |  |
500 ml | 22.00 | Add to Cart |
| RT | 11482-50 | Acetate Buffer, 0.2M, pH 5.0 | |
500 ml | 22.00 | Add to Cart |
| RT | 11482-56 | Acetate Buffer, 0.2M, pH 5.6 | |
500 ml | 22.00 | Add to Cart |
Acetic Acid, Glacial, Reagent A.C.S
C2H4O2 F.W. 60.05 CAS 64-19-7
| Assay min | 99.7% |
| RT | 10040 | Acetic Acid, Glacial | 500 ml | 59.00 | Add to Cart |
| RT | 10041 | Acetic Acid, Glacial | 2.5 L | 120.00 | Add to Cart |
Acetic Acid, Aqueous Solution
Prepared from acetic acid glacial with de-ionized water.
| RT | 10042-02 | Acetic Acid, 0.2% (v/v) Solution | |
500 ml | 19.00 | Add to Cart |
| RT | 10042-05 | Acetic Acid, 0.5% (v/v) Solution | |
500 ml | 20.00 | Add to Cart |
| RT | 10042-10 | Acetic Acid, 1.0% (v/v) Solution | |
500 ml | 22.00 | Add to Cart |
| RT | 10042-50 | Acetic Acid, 5.0% (v/v) Solution | |
500 ml | 30.00 | Add to Cart |
Acetone, Reagent, A.C.S.
CH3COCH3 F.W. 58.08 CAS #67-64-1
A.C.S. Specifications
| Assay | Min 99.5% | |
| Aldehyde (as HCHO) | 0.002% | |
| Color | Max. APHA 10 | |
| Density (G/ml) at 25°C | Max. 0.7857 | |
| Titratable Acid | 0.0003 meq/g | |
| Titratable Base | 0.0006 meq/g | |
| Isopropyl Alchohol | 0.05% | |
| Methanol (CH3OH) | 0.05% | |
| Residue after Evaporation | 0.001% | |
| Solubilty in water | To pass test | |
| Substances Reducing Permanganate |
To pass test | |
| Water | 0.5% |
| RT | 10000 | 1 qt. (946 ml) | 14.00 | Add to Cart | |
| RT | 10010 | 4 x 1 qt. | 49.00 | Add to Cart | |
| RT | 10012 | 250 ml | 8.00 | Add to Cart | |
| RT | 10014 | 4 x 250 ml | 26.00 | Add to Cart |
Acetone: Alcohol, 1:1 & Buffered Acetone
| RT | 10017 | Acetone: Alcohol, 1:1 | |
500 ml | 21.00 | Add to Cart |
| RT | 10018 | Buffered Acetone Fixative | |
500 ml | 25.00 | Add to Cart |
Acid Alcohols
An acidified alcohol solution for the differentiation of regressive stains.
| RT | 26071-01 | Acid Alcohol, 0.5% HCl in 70% Alcohol | |
1 L | 37.00 | Add to Cart |
| RT | 26071-04 | Acid Alcohol, 0.5% HCl in 70% Alcohol | 4 x 1 L | 138.00 | Add to Cart | |
| RT | 26072-01 | Acid Alcohol, 1% HCl in 70% Alcohol | |
1 L | 33.00 | Add to Cart |
| RT | 26072-04 | Acid Alcohol, 1% HCl in 70% Alcohol | 4 x 1 L | 120.00 | Add to Cart | |
| RT | 26073-01 | Acid Alcohol, 1% HCl in 95% Alcohol | |
1 L | 43.00 | Add to Cart |
| RT | 26073-04 | Acid Alcohol, 1% HCl in 95% Alcohol | 4 x 1 L | 160.00 | Add to Cart |
Ethyl Alcohol, Specially Denatured
C2H5OH F.W. 46.07 CAS #64-17-5
Blended 95% Ethanol and 5% Methanol.
Suitable for all routine histology/cytology preparations.
Specifications
| Evaporation Residue | 5ppm max. | |
| Water | 0.1% | |
| Filtered SDA-3A | 95% |
| RT | 15050 | 1 qt | 22.00 | Add to Cart | |
| RT | 15051 | 4 x 1 qt | 78.00 | Add to Cart |
Ethyl Alcohol, Anhydrous
(Ethanol) – 200 Proof
CH3CH2OH F.W. 46.07 CAS # 64-17-5
Specifications
| Specific Gravity | <0.7942 |
| Purity | >99.9% |
| Acidity | <0.002% |
| Water (Karl Fisher) | <0.1% |
| Non-Volatile Matter | <0.0025% |
| RT | 15055 | 450 ml | 20.00* | Add to Cart | |
| RT | 15056 | 4 x 450 ml | 70.00* | Add to Cart | |
| RT | 15058 | 1 liter | 35.00 | Add to Cart |
*Tax has already been paid
Alkaline Alcohol
| RT | 10380 | 10% NH4OH in 85.5% Alcohol | |
500 ml | 24.00 | Add to Cart |
| RT | 10381 | 1.6% NaCl in 80% Alcohol | |
500 ml | 38.00 | Add to Cart |
| RT | 10382 | 0.2% KOH in 80% Alcohol | |
500 ml | 40.00 | Add to Cart |
Ammonium Hydroxide, 28% Reagent, A.C.S.
NH4OH F.W. 35.05 CAS #1336-21-6 Specific Gravity: 0.91.
| Assay | 28-30% |
Specifications
| Residue After Ignition | 0.002% | |
| Carbon Dioxide | 0.002% | |
| Chloride | 0.5 ppm | |
| Phosphate | 2 ppm | |
| Sulfate | 2 ppm | |
| Heavy Metals | 0.5 ppm | |
| Iron | 0.2 ppm |
| RT | 10600 | 450 ml | 19.00 | Add to Cart | |
| RT | 10601 | 4 x 450 ml | 70.00 | Add to Cart |
Amphibian Ringer Solution
Ingredients: NaCl; KCl; CaCl2·6H2O and Distilled water.
| RT | 11762-05 | Amphibian Ringer Solution | 500 ml | 25.00 | Add to Cart |
| RT | 11762-10 | Amphibian Ringer Solution | 1 L | 35.00 | Add to Cart |
B-5 Fixative
For lymph nodes.
| RT | 64122-05 | B-5 Fixative | 500 ml | 64.00 | Add to Cart |
| RT | 64122-06 | B-5 Fixative | 1 lt | 120.00 | Add to Cart |
| RT | 64122-10 | B-5 Fixative | 4 lt | 450.00 | Add to Cart |
Borate Buffer
Please see the Buffer Section.
Bouin's Solution
Bouin's solution can be used as a fixation and a staining fluid. Bouin's fixative is excellent for use on biopsy specimens of the gastrointestinal tract. Tissue from the endocrine system are well fixed and many antibodies react well with tissue fixed in Bouin's. Bouin's solution may be used as a routine fixative or a staining solution, but should not be used as a preservative for tissue. Tissue cannot be held in Bouin's solution for extended periods. The maximum fixation time in this solution should be less than 48 hours and should be transferred to alcohol.
Packaged in leak-proof histo-containers, prefilled and ready-to-use.
Formulated with Picric Acid saturated aqueous solution 75%, Formalin (40% aqueous formaldehyde) 25% and Glacial Acetic acid 5% in de-ionized water.
| RT | 15990-01 | Bouin's Fluid | 1 L | 47.00 | Add to Cart |
| RT | 15990-04 | Bouin's Fluid | 4 L | 163.00 | Add to Cart |
| RT | 15990-10 | Bouin's Fluid Pre-filled cups | 50x10 ml/cs | 83.00 | Add to Cart |
| RT | 15990-20 | Bouin's Fluid Pre-filled cups | 50x20 ml/cs | 103.00 | Add to Cart |
| RT | 15990-30 | Bouin's Fluid Pre-filled cups | 20x30 ml/cs | 47.00 | Add to Cart |
Buffers
Acetate Buffer
| RT | 11482-42 | Acetate Buffer, 0.2M, pH 4.2 | |
500 ml | 23.00 | Add to Cart |
| RT | 11482-50 | Acetate Buffer, 0.2M, pH 5.0 | |
500 ml | 23.00 | Add to Cart |
| RT | 11482-56 | Acetate Buffer, 0.2M, pH 5.6 | |
500 ml | 23.00 | Add to Cart |
Antibody Dilution Buffer with Immunoglobulin-Free BSA
This ready-to-use Antibody Dilution Buffer is designed for the dilution of antibodies in all immunoassays, including immunofluorescence, IHC, ELISA, WB. This buffer contains immunoglobulin free BSA and does not contain phosphate, sodium azide, or mercury preservatives. It can be used for the dilution of all antibodies, including peroxidase, and antibodies to phophoproteins. However, it is not suitable for dilution of antibodies to S100 proteins. This buffer contains green food color.
Reagent Supplied: Ready to use buffer.
Application: Dilute antibodies, primary or secondary as recommended in the protocol by the manufacturer of these products.
Storage: 2-8°C. Diluted antibodies can be stored at 2-8°C for several weeks, depending upon the stability of antibodies.
| 2–8°C | 25886-01 | Antibody Dilution Buffer, free BSA | 100 ml | 62.00 | Add to Cart |
| 2–8°C | 25886-05 | Antibody Dilution Buffer, free BSA | 500 ml | 176.00 | Add to Cart |
| 1227SK | Antigen Preserve Solution VII | 1 L | 154.00 | Add to Cart |
Borate Buffer
| RT | 11455-78 | Borate Buffer, pH 7.8 | |
500 ml | 33.00 | Add to Cart |
| RT | 11455-82 | Borate Buffer, pH 8.2 | |
500 ml | 34.00 | Add to Cart |
| RT | 11455-90 | Borate Buffer, pH 9.0 | |
500 ml | 34.00 | Add to Cart |
Citrate Buffer
Citric acid and Sodium citrate
0.1M
pH range 4.6-6.2.
| 0-5°C | 11490 | Citrate Buffer, 0.1M | 225 ml | 18.00 | Add to Cart |
| 0-5°C | 11492 | Citrate Buffer, 0.1M | 500 ml | 23.00 | Add to Cart |
Michel's Buffer
| RT | 18041-05 | Michel's Buffer | 500 ml | 22.00 | Add to Cart |
| RT | 18041-10 | Michel's Buffer | 1 L | 29.00 | Add to Cart |
Millonig's Phosphate Buffer
Millonig (1964); Karlsson and Schultz (1965)
Ingredients:
Sodium Phosphate, monobasic (NaH2PO4xH2O)
Sodium Phosphate, dibasic (Na2HPO4xH2O)
0.5% Sodium Chloride (NaCl)
Shipping Conditions: Ambient Temperature
The pH of this buffer is 7.4 (0.1M); the osmolarity is 440 mosmols, so this buffer is hypertonic to most body fluids. Millonig recommended to use this buffer for the fixation of very hydrated tissues with osmium tetroxide. For marine organisms, a higher concentration of sodium chloride (3%) is recommended. We supply it in a double strength (0.2M), pH 7.4. Dilution is needed before use.
| 0-5°C | 11582-05 | Millonig's Phosphate Buffer 0.2M | 500 ml | 23.00 | Add to Cart |
| 0-5°C | 11582-10 | Millonig's Phosphate Buffer 0.2M | 1 L | 32.00 | Add to Cart |
PHEM Buffer
EMS is proud to announce and release of our newest Buffer for EM PHEM.
PHEM buffer has been used primarily for tissues and cell cultures being processed for immunocytochemical studies. Most antigens, especially intra cellular ones, stain better using PHEM than those processed using PBS, it also has a more limited effect on biochemical reactions and enzymes. Very good preservation of cellular ultrastructure, notably microtubules, is also an advantage of PHEM use.
References
- Schliwa, Manfred, and Jonathan Van Blerkom. "Structural interaction of cytoskeletal components." The Journal of Cell Biology 90.1 (1981): 222-235.
- Jacqueline Montanaro: Improved ultrastructure of marine invertebrates using non-toxic buffers
| 2-8°C | 11162 | PHEM Buffer | 225ml | 42.00 | Add to Cart |
| 2-8°C | 11163 | PHEM Buffer | 500ml | 74.00 | Add to Cart |
Phosphate Buffer, 0.1M*
Prepared from Sodium Phosphate Monobasic Hydrate and Sodium Phosphate Dibasic Heptahydrate. Non-Sterile. Meant for research purposes only.
| 19340-60 | Phosphate Buffer, pH 6.0 | |
1 L | 40.00 | Add to Cart | |
| 19340-65 | Phosphate Buffer, pH 6.5 | |
1 L | 40.00 | Add to Cart | |
| 19340-70 | Phosphate Buffer, pH 7.0 | |
1 L | 40.00 | Add to Cart | |
| 19340-72 | Phosphate Buffer, pH 7.2 | |
1 L | 40.00 | Add to Cart |
*Meant for biological research only. Not to be used for medical or any other purposes.
Phosphate Buffer Saline (PBS)
PBS is commonly used in biochemistry. It is a salty solution containing sodium chloride, potassium chloride, sodium phosphate and potassium phosphate. PBS is isotonic and non-toxic to cells. 1X PBS final concentration is 8.0 g/L NaCl, 0.2 g/L KCl, 1.42 g/L Na2HPO4, 0.24 g/L KH2PO4 and pH 7.4.
| RT | 19342-10 | Phosphate Buffered Saline, 10X | |
1 L | 32.00 | Add to Cart |
| RT | 19242-40 | Phosphate Buffered Saline, 10X | 1 gal | 98.00 | Add to Cart | |
| RT | 19343-10 | Phosphate Buffered Saline, 2X | |
1 L | 28.00 | Add to Cart |
| RT | 19343-40 | Phosphate Buffered Saline, 2X | 1 gal | 88.00 | Add to Cart | |
| RT | 19344-10 | Phosphate Buffered Saline, 1X | |
1 L | 24.00 | Add to Cart |
| RT | 19344-20 | Phosphate Buffered Saline, 1X | 1 gal | 76.00 | Add to Cart |
Sorensen's Phosphate Buffer
A Phosphate buffered mixed salt prepared from sodium phosphate monobasic and sodium phosphate dibasic.
| 0-5°C | 11600-10 | Sorensens Phosphate Buffer, 0.2M, pH 7.2 | |
1 L | 32.00 | Add to Cart |
| 0-5°C | 11600-40 | Sorensens Phosphate Buffer, 0.2M, pH 7.2 | 4 L | 110.00 | Add to Cart | |
| 0-5°C | 11601-10 | Sorensens Phosphate Buffer, 0.2M, pH 7.4 | |
1 L | 33.00 | Add to Cart |
| 0-5°C | 11601-40 | Sorensens Phosphate Buffer, 0.2M, pH 7.4 | 4 L | 110.00 | Add to Cart |
Tris, (hydroxymethyl) aminomethane maleate
Tris Maleate Buffer
| 0-5°C | 11730 | Tris Maleate Buffer 0.2M pH range 6.4-8.0 | 250 ml | 23.00 | Add to Cart |
| 0-5°C | 11740 | Tris Maleate Buffer 0.2M pH range 6.4-8.0 | 500 ml | 30.00 | Add to Cart |
Tris Buffer
| RT | 11730-05 | Tris Buffer 0.2M pH 8.0 | 250 ml | 23.00 | Add to Cart |
| RT | 11730-06 | Tris Buffer 0.2M pH 8.0 | 500 ml | 30.00 | Add to Cart |
Tris Buffered Saline
| RT | 11730-08 | Tris Buffered Saline pH 7.4 | 250 ml | 23.00 | Add to Cart |
| RT | 11730-09 | Tris Buffered Saline pH 7.4 | 500 ml | 30.00 | Add to Cart |
Tris-HCL Buffer
| RT | 11730-12 | Tris-HCL Buffer 1M pH range 6-8 | 250 ml | 23.00 | Add to Cart |
| RT | 11730-15 | Tris-HCL Buffer 1M pH range 6-8 | 500 ml | 30.00 | Add to Cart |
Tris HCL Buffer
| RT | 11730-20 | Tris HCL Buffer pH range 7.2-9.0 | 250 ml | 23.00 | Add to Cart |
| RT | 11730-25 | Tris HCL Buffer pH range 7.2-9.0 | 500 ml | 30.00 | Add to Cart |
Universal Immunoassay Buffer™, 10X
Immunoassay buffer is used as a 1X in all immunoassays (immunofluorescence, IHC, ELISA, WB etc.) as a washing buffer and it can be used as a dilution buffer for antibodies; however it does not contain any carrier proteins.
This buffer also does not contain any phosphate, sodium azide or mercury as preservatives and can be used for dilution of all antibodies, including peroxidase, antibodies to phosphoproteins. It is not suitable for dilution of antibodies to S100 proteins.
Reagent Supplied: 10X Buffer.
Application: Dilute this 10X buffer 10 times with DI water. (90 ml water + 10 ml this buffer – mixed well). Wash 3-5 times with this buffer in between the steps of your immunoassay protocol.
Storage: Room temperature.
| RT | 25880-01 | Universal Immunoassay Buffer, 10X | 100 ml | 58.00 | Add to Cart |
| RT | 25880-10 | Universal Immunoassay Buffer, 10X | 1 L | 160.00 | Add to Cart |
Universal Antibody Dilution Buffer™
This ready-to-use Universal Antibody Dilution Buffer is designed for dilution of antibodies in all immunoassays, including immunofluorescence, IHC, ELISA, WB. This buffer does not contain any mammalian proteins, phosphate, sodium azide, or mercury preservatives and can be used for dilution of all antibodies, including peroxidase, and antibodies to phophoproteins. However, it is not suitable for dilution of antibodies to S100 proteins.
Reagent Supplied: Ready to use buffer.
Application: Dilute antibodies, primary or secondary as recommended in the protocol by the manufacturer of these products.
Storage: 2-8°C. Diluted antibodies can be stored at 2-8°C for several weeks, depending upon the stability of antibodies.
| 2-8°C | 25885-01 | Universal Antibody Dilution Buffer | 100 ml | 58.00 | Add to Cart |
| 2-8°C | 25885-05 | Universal Antibody Dilution Buffer | 500 ml | 162.00 | Add to Cart |
n-Butyl Alcohol, Reagent, A.C.S.
(Butanol)
CH3CH2CH2CH2OH F.W. 74.12 CAS # 71-36-3
| Specific Gravity: | 0.81 | |
| Assay | 99.4% | |
| Residue after evaporation | 0.005% | |
| Titratable acid | 0.0008meq/g | |
| Butyl Ether | 0.2% | |
| Water | 0.1% |
| RT | 11920 | 450 ml | 30.00 | Add to Cart | |
| RT | 11921 | 4x450 ml | 104.00 | Add to Cart |
Carnoy's Fixatives
Stock solution.
| RT | 64130-05 | Carnoy's Fluid | |
500 ml | 26.00 | Add to Cart |
| RT | 64130-50 | Carnoy's Fluid Without Chloroform | |
500 ml | 24.00 | Add to Cart |
Cedar Wood Oil
CAS #8000-27-9 Refractive Index @ 20°C 1.504+/-.002
For clearing.
| RT | 12420 | 100 ml | 60.00 | Add to Cart |
Chloroform, Reagent, A.C.S.
CHCl3 FW 119.38 CAS #67-66-3
A.C.S. Specifications
| Color (APHA) | 10 | |
| Density @ 25°C | 1.471-1.476 | |
| Residue after Evaporation | 0.001% | |
| Acetone & Aldehyde (about 0.00%) | To Pass Test | |
| Acid & Chloride | To Pass Test | |
| Free Chlorine (Cl) | To Pass Test | |
| Lead (Pb) | 0.05ppm | |
| Subs. darkened by H2SO4 | To Pass Test | |
| Suitability for Dithizone Test | To Pass Test |
| RT | 12540 | 450 ml | 18.00 | Add to Cart | |
| RT | 12541 | 4x450 ml | 66.00 | Add to Cart |
Chromic Acid, Reagent, Crystals, A.C.S.
CrO3 FW 99.99 CAS #1333-82-0
A.C.S. Specifications
| Chloride (Cl) | 0.005% | |
| Insoluble Matter | 0.01% | |
| Nitrate (NO3) | 0.05% | |
| Sodium (Na) | 0.2% | |
| Iron, Aluminum, Barium | 0.03% | |
| Sulfate (SO4) | 0.05% | |
| Assay | 98% |
| RT | 12555 | 100g | 45.00 | Add to Cart |
Chromic Acid 4% Aqueous Solution
| RT | 12557 | Chromic Acid 4% Aqueous Solution | |
500 ml | 30.00 | Add to Cart |
| RT | 12558 | Chromic Acid 5% Aqueous Solution | |
500 ml | 34.00 | Add to Cart |
Clearing Solvent Xylene Substitutes
These clearing solvents offer Histology and Cytology laboratories a safer alternative to xylene.
- Contains no xylene or toluene
- No citrus odor
- For use in manual and automated systems
| RT | 64129-10 | Clearing Solvent | 1 gal | 52.00 | Add to Cart |
RDO Rapid Decalcifier
RDO and RDO Gold were developed with the basic premise of making your work routine quicker and easier without sacraficing the quality and integrity of your samples.
RDO and RDO Gold are remarkably effective multipurpose decacifiers that can be tailored to suit your specific lab routine.
Both RDO and RDO Gold
- Offer crisp nuclear staining and definition
- Give distinguishable chromatin
- Show great surface decalcification
- Safe enough to be disposed down to lab sink to drain
How is RDO Gold different from the original RDO
- RDO Gold is designated for those biopsies which contain minimal amounts of calcium
- No filtration is required with RDO Gold
- RDO Gold gives stellar morphological representation of bone biopsies
- There is no folding of tissues with RDO Gold
- RDO Gold is clear and non-precipitate
Both RDO and RDO Gold are
- Far Superior to other Decalcifiers
- Great Cellular Differentiation
- Safe – Non Toxic – Non-Corrosive
- Dilutable
- Microwavable
- Superb Staining Quality
- Biodegradable
- Translucent
- Available in Wide Mouth Liter Bottle
References
- Gayle Callis et al. "Dacalcification of Bone: Literature Review and Practical Study of Various Decalcifying Agents, Methods, and Their Effects on Bone Histology". The J. of Histology, Vol. 21, No. 1, March 1998
- Sherley A. Powell, HT (ASCP) HTL. "Method to Facilitate Sectioning Incompletely Decalcified Bone". Mercer University, School of Medicine. Microtime Georgia, State Newsletter
- Becky Scholes, HTL, MT (ASCP).:Bone Marrow Biopsies". H.I.S.T.O. Iowa State Newsletter
| RT | 64143-01 | RDO Decalcifier | |
1 L | 26.00 | Add to Cart |
| RT | 64143-06 | RDO Decalcifier | 6 x 1 L/cs | 126.00 | Add to Cart | |
| RT | 64143-40 | RDO Decalcifier | 1 gal | 56.00 | Add to Cart | |
| RT | 64143-44 | RDO Decalcifier | 4 x 1 gal/cs | 195.00 | Add to Cart | |
| RT | 64144-01 | RDO – Gold Decalcifier | |
1 L | 44.00 | Add to Cart |
| RT | 64144-06 | RDO – Gold Decalcifier | 6 x 1 L/cs | 245.00 | Add to Cart | |
| RT | 64144-40 | RDO – Gold Decalcifier | 1 gal | 84.00 | Add to Cart | |
| RT | 64144-44 | RDO – Gold Decalcifier | 4 x 1 gal/cs | 299.00 | Add to Cart |
Technical Tips and Articles
RDO Rapid Decalcifier
Animal Tissue Techniques
Parafin and Plastic Techniques for Preparation of Bone Marrow Specimen
Bone Marrow Biopsies
Decalcifier
- Ready-to-use
- For hard specimens
- Protects cellular structure
- Minimal interference with subsequent staining
- For use with human and animal calcified tissue, bone, cartilage and other hard specimens
| RT | 64147-01 | Decalcifier | 1 L | 32.00 | Add to Cart |
| RT | 64147-06 | Decalcifier | 6 x 1 L | 138.00 | Add to Cart |
| RT | 64147-10 | Decalcifier | 10 L | 179.00 | Add to Cart |
Deionized Water, Reagent Grade A.C.S.
H2O F.W. 18.02 CAS #7732-18-5
EMS Reagent Grade Water is typically prepared at 18 megohm/cm specific resistance using a reverse osmosis, mixed deionization, activated filtration and final filtration at 0.2 microns.
| Color (APHA) | <+/-5 | |
| Bacteriological purity | 0 CFUs/L | |
| Residue after evaporation | 10ppm | |
| Coliform | negative |
| RT | 22800-01 | Deionized Water | 1 gal | 12.00 | Add to Cart |
| RT | 22800-05 | Deionized Water | 5 gal | 50.00 | Add to Cart |
EMS Rapid Pro Processing System
A Revolutionary Rapid Tissue Processing System
Tissue processing has not changed in more than a century... until now. The EMS Rapid Pro Processing System can cut total processing time to as little as 46 minutes.
Advantages over traditional processing
- Speed – no other processing chemicals will deliver results this fast
- Increased throughput
- Suitable for both fresh and frozen sections
- Catalyzed versions of standard processing chemicals eliminate the need for new safety features, storage, or learning curves
- Effective for manual or automatic tissue processing
- Compatible with existing tissue processing equipment
- "Results While You Wait" option for patients
For more staining protocols, see the Technical Data Sheet.
A proven and reliable method for obtaining rapid results in tissue processing
| Process | Standard Processing Time | EMS Rapid Pro Processing Time |
| Formalin | ½ hr - 1 hr | 3 min |
| 70% Ethanol | 1 hr | 3 min |
| 95% Ethanol | 1 hr | 3 min |
| 98% Ethanol | 1 hr | 3 min |
| 90% Xylenes | ½ hr - 1 hr | 3 min |
| 98% Ethanol | 1 hr | 3 min |
| 90% Xylenes | ½ hr - 1 hr | 3 min |
| Paraffin | 2 - 3 hrs | 25 min |
| Total Processing TIme | 7½ - 10 hrs | 46 min |
Ordering
| 26750-10 | EMS Rapid ProKit | kit | 36.00 | Add to Cart | |
| 26750-01 | EMS Rapid Pro Solution 1 | |
250 ml | 12.00 | Add to Cart |
| 26750-02 | EMS Rapid Pro Solution 2 | |
250 ml | 11.00 | Add to Cart |
| 26750-03 | EMS Rapid Pro Solution 3 | |
250 ml | 4.00 | Add to Cart |
| 26750-04 | EMS Rapid Pro Solution 4 | |
250 ml | 4.00 | Add to Cart |
| 26750-05 | EMS Rapid Pro Solution 5 | |
250 ml | 9.00 | Add to Cart |
Ferric Ammonium Sulfate
| RT | 15505-40 | Ferric Ammonium Sulfate, 4% Aqueous | |
500 ml | 31s.00 | Add to Cart |
| RT | 15505-50 | Ferric Ammonium Sulfate, 5% Aqueous | |
500 ml | 32.50 | Add to Cart |
Ferric Chloride, Hexahydrate, Reagent, A.C.S.
FeCl3·6H2O F.W. 270.30 CAS #13478-10-9
Specifications
| Assay | 97.0-102% |
| Insoluble Matters. | 0.01% |
| Nitrate (about 0.001%) | To Pass Test |
| Phosphorous Compound | 0.01% |
| Sulfate | 0.01% |
| Arsenic | 0.002% |
| Copper | 0.003% |
| Zinc | 0.003% |
References for use
Membrane stains
- Basic, G., Berwick et al (1963). Hale stain for sialic acid containing mucins. J. Cell Biol., 19:223
- Gasic, G., Berwick, et al (1968). Positive and Negative colloidal iron as cell surface electron stains. Lab. Invest., 18:63
- Blanquet, P.R. and Loiez, A. (1974). Colloidal iron used at pHs lower than 1 as electron stain for surface proteins. J. Histochem. Cytochem., 22:368
- Matukafs, V.J., Panner et al (1967). Studies on ultrastructural identification and distribution of protein-polysaccharides in cartilage matrix. J. Cell Biol., 32:365
- Benedetti, E.L. and Emmelot, P. (1967). Studies on plasma membranes IV. The Ultrastructural localization and content of sialic acid in plasma membranes isolated from rat liver and hepatoma. J. Cell Sci., 2:499
- Rowley, J.R. (1971). Resolution of channels in the exine by translocation of colloidal iron. Pro. 29th Ann. EMSA Meet., p.352. Claitor's Pub. Division, Baton Rouge, LA
- Nicolson, G.L. (1973). Anionic sites of human erythrocyte membranes.I. Effects of trypsin, phospholipase C, and pH on the topography of bound positively charged colloidal particles. J. Cell Biol., 57:373
- Hendy, R. (1971). Electron microscopy of lipofucsin pigment stained by the Schmorl and Fontana technique. Histochemie, 26:311
| RT | 15510 | Ferric Chloride, Hexahydrate, Reagent | 500g | 45.00 | Add to Cart |
Ferric Chloride 10%, Aqueous Solution
| RT | 15512-02 | Ferric Chloride 2% Aqueous | |
500 ml | 37.00 | Add to Cart |
| RT | 15512-10 | Ferric Chloride 10% Aqueous | |
500 ml | 37.00 | Add to Cart |
Fixatives
B-5 Fixative
For lymph nodes.
| RT | 64122-05 | B-5 Fixative | 500 ml | 64.00 | Add to Cart |
| RT | 64122-06 | B-5 Fixative | 1 lt | 120.00 | Add to Cart |
| RT | 64122-10 | B-5 Fixative | 4 lt | 450.00 | Add to Cart |
Bouin's Solution
Bouin's solution can be used as a fixation and a staining fluid. Bouin's fixative is excellent for use on biopsy specimens of the gastrointestinal tract. Tissue from the endocrine system are well fixed and many antibodies react well with tissue fixed in Bouin's. Bouin's solution may be used as a routine fixative or a staining solution, but should not be used as a preservative for tissue. Tissue cannot be held in Bouin's solution for extended periods. The maximum fixation time in this solution should be less than 48 hours and should be transferred to alcohol.
Packaged in leak-proof histo-containers, prefilled and ready-to-use.
Formulated with Picric Acid saturated aqueous solution 75%, Formalin (40% aqueous formaldehyde) 25% and Glacial Acetic acid 5% in de-ionized water.
| RT | 15990-01 | Bouin's Fluid | 1 L | 47.00 | Add to Cart |
| RT | 15990-04 | Bouin's Fluid | 4 L | 163.00 | Add to Cart |
Bouin's Fluid in Pre-filled cups.
| RT | 15990-10 | Bouin's Fluid | 50 x 10 ml/cs | 83.00 | Add to Cart |
| RT | 15990-20 | Bouin's Fluid | 50 x 20 ml/cs | 103.00 | Add to Cart |
| RT | 15990-30 | Bouin's Fluid | 20 x 30 ml/cs | 47.00 | Add to Cart |
Brasil Fixative
Ethanol, Picric Acid, Formalin, Acetic Acid, Glacial.
This fixative is most popular when glycogen is the target. The solution is similar to Bouins Solution (alcoholic) offering quick hardening and good staining with acid dyes.
| RT | 15995-10 | Brasil Fixative | 10x10 ml | 42.00 | Add to Cart |
| RT | 15995-20 | Brasil Fixative | 100 ml | 42.00 | Add to Cart |
Buffered Ethanol Fixative, 70% BE-70
EMS is proud to introduce this ready-to-use new fixative designed to offer the latest preservation of structural integrity with good immunostaining results. This fixative is a phosphate buffered ethanol (BE-70) with the addition of glycerol and glacial acetic acid.
Reference: Journal of Histochemistry & Cytochemistry 2017, Vol 65(4) p. 251-252
| RT | 15988 | 1 L | 70.00 | Add to Cart |
Carnoy's Fixatives
Stock solution.
| RT | 64130-05 | Carnoy's Fluid | |
500 ml | 26.00 | Add to Cart |
| RT | 64130-50 | Carnoy's Fluid Without Chloroform | |
500 ml | 24.00 | Add to Cart |
Carson's Phosphate Buffered Formalin
Millonig's phosphate buffered formalin as modified by Carson, Martin and Lynn, by using 4% formalin in 0.1M sodium phosphate monobasic with sodium hydroxide to pH 7.2.
| RT | 15732-05 | Carson's Phosphate Buffered Formalin | 500 ml | 20.00 | Add to Cart |
| RT | 15732-10S | Carson's Phosphate Buffered Formalin | 1 L | 33.00 | Add to Cart |
Carson's Phosphate Buffered Paraformaldehyde
For some tissues, the use of formalin (contains 10-15% Methanol) is not suitable, we offer the alternative formaldehyde (prepared from paraformaldehyde – contains no methanol) for your convenience.
| RT | 15733-05 | Carson's Phosphate Buffered Paraformaldehyde | 500 ml | 23.00 | Add to Cart |
| RT | 15733-10 | Carson's Phosphate Buffered Paraformaldehyde | 1 L | 37.00 | Add to Cart |
CytoPrep™ Fixative
CytoPrep Fixative forms a clear protective coating over specimens while fixing cells. It is ideal for cytological smears and it dries within 4 to 6 minutes. Good for safe storage and transportation of slides. It is water soluble and contains no chlorofluorocarbons. Comes in a 4 oz. spray can.
| RT | 64145-01 | CytoPrep Fixative | 4 oz | 40.00 | Add to Cart |
| RT | 64145-12 | CytoPrep Fixative | 12 x 4 oz | 410.00 | Add to Cart |
CytoFix
Cytology fixative spray designed for slide based cytology specimens for cellular preservation and transport.
Quickly spray a fine mist over the slide (covering the entire specimen) for cellular fixation and reduce air drying of the material.
CytoFix forms a thin, tough, clear protective coating over the specimen with a water and alcohol solution of Polyethylene glycol for cellular fixation and does not require rehydration by the laboratory prior to staining. Allows safe storing or transporting of slides.
Allow specimens to dry before sending to lab or for storage.
- Dries in 4 to 6 minutes
- Water soluble
- Won't cloud or obscure specimen
- Contains no chlorofluorocarbons
Available in pump bottle or aerosol can.
| 22805-01 | CytoFix (Non-Aerosol) | 100 ml | 14.50 | Add to Cart |
| 22805-02 | CytoFix Can | 4 oz | 14.50 | Add to Cart |
Davidson's Fixative (Hartmann's/ Davidson's Fixative)
This is rapid fixative. Results with good nuclear detail and minimal formalin pigment. Davidson's (Hartmann's) Fixative is useful for preparing tumors, bone marrow, gynecologic specimen, fatty breast, medical biopsies. Fixation of small specimen is rapid. Exposure to the fixative should be limited to 24 hours (tissue may then be transferred to neutral buffered formalin or 70% alcohol for storage).
Based on the volume of 1125 ml solution, there are 2 parts Formalin (35-39%), 3 parts Ethanol, 1 part Glacial Acetic acid, and 3 parts tap water.
| RT | 64133-10 | Davidson's (Hartmann's) Fixative | 1 L | 82.00 | Add to Cart |
| RT | 64133-14 | Davidson's (Hartmann's) Fixative | 4 L | 232.00 | Add to Cart |
Modified Davidson's Fixative
(14% Ethyl Alcohol, Denatured, 37.5% Formalin 37-39%, 6.25% Glacial Acetic Acid, and D.I. Water).
| RT | 64133-50 | Modified Davidson's Fixative | 1 L | 39.00 | Add to Cart |
Dietrich's Fixative
Ethanol, formalin, glacial acetic acid, distilled water.
| RT | 15958-10 | Dietrich's Fixative | 10 x 10 ml | 45.00 | Add to Cart |
| RT | 15958-20 | Dietrich's Fixative | 100 ml | 44.00 | Add to Cart |
Fixative/Decalcifier
- A solution that fixes and decalcifies in one step
- Prevents loss of cellular structure
- Enhances hematoxylin staining
| RT | 64148-01 | Fixative/Decalcifier | 1 L | 52.00 | Add to Cart |
| RT | 64148-06 | Fixative/Decalcifier | 6 x 1 L | 310.00 | Add to Cart |
Formalde-Fresh Low Odor Solution; Histology Grade
A specially formulated odor-mask which neutralizes the formalin odor without affecting subsequent tissue processing, handling, or staining. Low odor, ready-to-use 10% formalin solution. May be used as a fixative or preservative of specimens. Its shelf life is 24 months.
| RT | 15678-01 | Formalde-Fresh | 4 L | 95.00 | Add to Cart |
| RT | 15678-04 | Formalde-Fresh | 4 x 4 L | 364.00 | Add to Cart |
Buffered Formalde-Fresh, Low-Odor 10% Formalin Solution
Low odor, prepared w/v, pH 6.9 to 7.1 at 25°C. 10% formalin in Phosphate buffer according to the A.F.I.P. Manual of Histology. This solution allows for long-term storage of tissue specimens.
| RT | 15741-01 | Low-Odor Formalin 10% Buffered | 4 L | 65.00 | Add to Cart |
| RT | 15741-04 | Low-Odor Formalin 10% Buffered | 4 x 4 L | 239.00 | Add to Cart |
Formalin, Neutral, Buffered 10% w/v in Acetate Buffer
Buffered with Sodium Acetate in accordance with the A.F.I.P. manual of Histology. Filtered and ready-to-use.
pH 6.0 to ph 7.1 at 20°C.
| RT | 15738-01 | Formalin 10% Buffered in Acetate | 1 L | 24.00 | Add to Cart |
| RT | 15738-04 | Formalin 10% Buffered in Acetate | 4 L | 56.00 | Add to Cart |
Formalin, Neutral, Buffered 10% w/v in Phosphate Buffer
Buffered with phosphate in accordance with the A.f.I.P. manual of Histology. Filtered and ready-to-use.
pH 6.9 to 7.1 at 25°C.
| RT | 15740 | Formalin 10% Buffered in Phosphate | 450 ml | 17.00 | Add to Cart |
| RT | 15740-01 | Formalin 10% Buffered in Phosphate | 1 L | 24.00 | Add to Cart |
| RT | 15740-04 | Formalin 10% Buffered in Phosphate | 4 L | 62.00 | Add to cart |
Formalin 1% Non Buffered
| RT | 15734-01 | Formalin 1% Non-Buffered | 1 L | 22.00 | Add to Cart |
| RT | 15734-04 | Formalin 1% Non-Buffered | 4 L | 57.00 | Add to Cart |
Formalin 2%, Non-Buffered
| RT | 15735-01 | Formalin 2% Non-Buffered | 1 L | 22.00 | Add to Cart |
| RT | 15735-04 | Formalin 2% Non-Buffered | 4 L | 57.00 | Add to Cart |
Formalin 3%, Non-Buffered
| RT | 15735-10 | Formalin 3% Non-Buffered | 1 L | 23.00 | Add to Cart |
| RT | 15735-20 | Formalin 3% Non-Buffered | 4 L | 58.00 | Add to Cart |
Formalin 4%, Non-Buffered
| RT | 15735-30 | Formalin 4% Non-Buffered | 1 L | 23.00 | Add to Cart |
| RT | 15735-40 | Formalin 4% Non-Buffered | 4 L | 57.00 | Add to Cart |
Formalin 5%, Non-Buffered
| RT | 15735-50 | Formalin 5% Non-Buffered | 1 L | 24.00 | Add to Cart |
| RT | 15735-60 | Formalin 5% Non-Buffered | 4 L | 58.00 | Add to Cart |
TissuePrep Buffered 10% Formalin
Prefilled with 10% formalin Cat# 15740. Ready-to-use in pre-filled cups. Labeled in accordance to OSHA requirements, which gives you immediate information regarding handling and first aid procedures. Pre-measured in wide-mouth, leak-proof, opaque polypropylene containers which limits your exposure to formaldehyde and saves you time.
| Catalog # | Cup Size | Filled | Pack/cs | Price | ||
| RT | 15742-10 | 15 ml | 10 ml | 50 | 77.00 | Add to Cart |
| RT | 15742-20 | 30 ml | 20 ml | 50 | 98.00 | Add to Cart |
| RT | 15742-30 | 60 ml | 30 ml | 20 | 44.00 | Add to Cart |
| RT | 15742-60 | 120 ml | 60 ml | 20 | 48.00 | Add to Cart |
Paraformaldehyde, Prepared Fixatives
In addition to our complete line of Formaldehyde solutions we have now added to the line.
Paraformaldehyde 0.5% in 0.1M phosphate buffer pH7.4
| RT | 15951-10 | Paraformaldehyde, 0.5% each in 0.1M Phosphate Buffer | 10 x 10 ml | 39.00 | Add to Cart |
| RT | 15951-15 | Paraformaldehyde, 0.5% each in 0.1M Phosphate Buffer | 500 ml | 105.00 | Add to Cart |
Paraformaldehyde 1% Aqueous
| RT | 15953-10 | Paraformaldehyde, 1% each Aqueous | 10 x 10 ml | 27.00 | Add to Cart |
| RT | 15953-15 | Paraformaldehyde, 1% each Aqueous | 500 ml | 97.00 | Add to Cart |
Paraformaldehyde 1% in 0.1M Phosphate Buffer pH, 7.4
| RT | 15952-10 | Paraformaldehyde, 1% each in 0.1M Phosphate Buffer | 10 x 10 ml | 41.00 | Add to Cart |
| RT | 15952-15 | Paraformaldehyde, 1% each in 0.1M Phosphate Buffer | 500 ml | 105.00 | Add to Cart |
Paraformaldehyde 1% Glut 2% in 0.1M Phosphate buffer, pH 7.4
| RT | 15949-30 | Paraformaldehyde 1% Glutaraldehyde 2% in 0.1M Phosphate Buffer | 10 x 10 ml | 41.00 | Add to Cart |
| RT | 15949-35 | Paraformaldehyde 1% Glutaraldehyde 2% in 0.1M Phosphate Buffer | 500 ml | 108.00 | Add to Cart |
Paraformaldehyde 3% Glutaraldehyde 0.35% in 0.1M Sodium Cacodylate, pH 7.4
| RT | 15949-50 | Paraformaldehyde 3% Glutaraldehyde 0.35% in 0.1M Sodium Cacodylate Buffer | 10 x 10 ml | 44.00 | Add to Cart |
| RT | 15949-60 | Paraformaldehyde 3% Glutaraldehyde 0.35% in 0.1M Sodium Cacodylate Buffer | 500 ml | 115.00 | Add to Cart |
Paraformaldehyde 4% and Glutaraldehyde 1% in Phosphate Buffer, pH 7.0
| RT | 15949-70 | Paraformaldehyde 4% Glutaraldehyde 1% in 0.1M Phosphate Buffer | 10 x 10 ml | 48.00 | Add to Cart |
| RT | 15949-80 | Paraformaldehyde 4% Glutaraldehyde 1% in 0.1M Phosphate Buffer | 500 ml | 115.00 | Add to Cart |
Paraformaldehyde 4% in 0.1M Sodium Cacodylate, pH7.4
| RT | 15952-10S | Paraformaldehyde 4% in 0.1M Sodium Cacodylate Buffer | 10 x 10 ml | 51.00 | Add to Cart |
| RT | 15952-15S | Paraformaldehyde 4% in 0.1M Sodium Cacodylate Buffer | 500 ml | 131.00 | Add to Cart |
Paraformaldehyde 4% in 0.1M Phosphate Buffer, pH 7.4
| RT | 15735-10S | Paraformaldehyde 4% in 0.1M Phosphate Buffer | 10 x 10 ml | 39.00 | Add to Cart |
| RT | 15735-20S | Paraformaldehyde 4% in 0.1M Phosphate Buffer | 500 ml | 109.00 | Add to Cart |
Paraformaldehyde 4% in 0.2M Phosphate Buffer, pH 7.4
| RT | 15735-30S | Paraformaldehyde 4% in 0.2M Phosphate Buffer | 10 x 10 ml | 38.00 | Add to Cart |
| RT | 15735-40S | Paraformaldehyde 4% in 0.2M Phosphate Buffer | 500 ml | 108.00 | Add to Cart |
Paraformaldehyde 4% in 0.1M Phosphate Buffer, pH 7.2
| RT | 15735-50S | Paraformaldehyde 4% in 0.1M Phosphate Buffer | 10 x 10 ml | 44.00 | Add to Cart |
| RT | 15735-60S | Paraformaldehyde 4% in 0.1M Phosphate Buffer | 500 ml | 112.00 | Add to Cart |
Paraformaldehyde 4% in 0.2M Phosphate Buffer, pH 7.2
| RT | 15735-70 | Paraformaldehyde 4% in 0.2M Phosphate Buffer | 10 x 10 ml | 44.00 | Add to Cart |
| RT | 15735-80 | Paraformaldehyde 4% in 0.2M Phosphate Buffer | 500 ml | 110.00 | Add to Cart |
Paraformaldehyde 4% in 0.1M Phosphate BufferSaline, pH7.4
| RT | 15735-85 | Paraformaldehyde 4% in 0.1M Phosphate Buffer Saline | 10 x 10 ml | 45.00 | Add to Cart |
| RT | 15735-90 | Paraformaldehyde 4% in 0.1M Phosphate Buffer Saline | 500 ml | 114.00 | Add to Cart |
Paraformaldehyde 4% in 0.2M Phosphate Buffer Saline, pH 7.4
| RT | 15735-95 | Paraformaldehyde 4% in 0.2M Phosphate Buffer | 10 x 10 ml | 46.00 | Add to Cart |
| RT | 15735-100 | Paraformaldehyde 4% in 0.2M Phosphate Buffer | 500 ml | 115.00 | Add to Cart |
Formaldehyde, Reagent, A.C.S. (Formalin)
HCHO F.W. 30.03 CAS #50-00-0
| Specific Gravity | 1.09 |
A.C.S. Specifications
| Assay | 35.5 to 38.0% | |
| Residue after ignition | 0.005% | |
| Color (A.P.H.A.) | 10 | |
| Sulfate (SO4) | 0.002% | |
| Heavy Metal (as Pb) | 5ppm | |
| Titrate Acid | 0.006 meq/g | |
| Chloride (Cl) | 5ppm | |
| Iron (Fe) | 5ppm |
| RT | 15680 | 450 ml | 16.00 | Add to Cart | |
| RT | 15681 | 4 x 450 ml | 54.00 | Add to Cart | |
| RT | 15682 | 1 qt (946 ml) | 25.00 | Add to Cart |
Formaldehyde, 37%, Microfiltered (Formalin Microfiltered)
HCHO F.W. 30.03 CAS# 50-00-0
Microfiltered with a 0.45 micron membrane; suitable as an EM fixative when a methanol free solution is not necessary.
| RT | 15686 | 10 x 10 ml | 34.00 | Add to Cart |
Formaldehyde
(Paraformaldehyde) GLUTARALDEHYDE
Karnovsky's Fixative Kit, please click here.
| RT | 15720 | kit | 28.00 | Add to Cart | |
| RT | 15730 | Lot of 5 kits | 126.00 | Add to Cart |
Fixative Reagent Grade (Karnovsky's)
This fixative contains 3% glutaraldehyde and 2% formaldehyde in 0.1M phosphate buffer, pH 7.4.
Karnovsky's with formalin 2% and Biological glutaraldehyde 3% in 0.1m Phosphate Buffer.
| RT | 15731-10 | Karnovsky Fixative Reagent Grade | 1 L | 260.00 | Add to Cart |
Karnovsky's with paraformaldehyde solution and EM grade glutaraldehyde
Please Note: This kit can be formulated to fit your requirements. Please contact us.
| RT | 15732-10 | Karnovsky Fixative EM Grade | 1 L | 286.00 | Add to Cart |
Formaldehyde Aqueous Solution (Paraformaldehyde Aqueous Solution) EM Grade
Paraformaldehyde – methanol free solution. A more efficient and rapid penetrant fixative used in combination with Glutaraldehyde and Acrolein fixatives. Easy to break, prescored, 10 ml ampoules sealed under inert gas, or 100 ml bottles.
CONTAINS NO PRESERVATIVES
| RT | 15700 | 16% Paraformaldehyde (formaldehyde) aqueous solution | |
10 ml | 6.00 | Add to Cart |
| RT | 15710 | 16% Paraformaldehyde (formaldehyde) aqueous solution | |
10x10 ml | 31.00 | Add to Cart |
| RT | 15710-S | 16% Paraformaldehyde (formaldehyde) aqueous solution | 100 ml bottle | 30.00 | Add to Cart | |
| RT | 15711 | 16% Paraformaldehyde (formaldehyde) aqueous solution | 10 bxs/lot | 280.00 | Add to Cart | |
| RT | 15712 | 10% Paraformaldehyde (formaldehyde) aqueous solution | |
10x10 ml | 30.00 | Add to Cart |
| RT | 15712-S | 10% Paraformaldehyde (formaldehyde) aqueous solution | 100 ml | 29.00 | Add to Cart | |
| RT | 15713 | 20% Paraformaldehyde (formaldehyde) aqueous solution | |
10x10 ml | 34.00 | Add to Cart |
| RT | 15713-S | 20% Paraformaldehyde (formaldehyde) aqueous solution | 100 ml | 33.00 | Add to Cart | |
| RT | 15714 | 32% Paraformaldehyde (formaldehyde) aqueous solution | |
10x10 ml | 39.00 | Add to Cart |
| RT | 15714-S | 32% Paraformaldehyde (formaldehyde) aqueous solution | 100 ml | 38.00 | Add to Cart |
In addition to our complete line of Formaldehyde solutions shown above we have now added to the line.
Since the DOT (Department of Transportation) has changed the definition of what a non dangerous solution of formaldehyde is to under 10%, we are now offering an 8% and a 4% solution. This will allow us to ship overnight as well as overseas without any hazardous charges saving our customers a good deal of money.
The quality of our 8% and 4% aqueous Paraformaldehyde solution is as good as all of the other concentrations that we offer.
CONTAINS NO PRESERVATIVES
| RT | 157-4 | 4% Paraformaldehyde (formaldehyde) aqueous solution | |
10 x 10 ml | 27.00 | Add to Cart |
| RT | 157-4-100 | 4% Paraformaldehyde (formaldehyde) aqueous solution | 100 ml | 26.00 | Add to Cart | |
| RT | 157-8 | 8% Paraformaldehyde (formaldehyde) aqueous solution | |
10 x 10 ml | 26.00 | Add to Cart |
| RT | 157-8-100 | 8% Paraformaldehyde (formaldehyde) aqueous solution | 100 ml | 25.00 | Add to Cart |
Formaldehyde/Zinc Fixative
Prepared: Ready-to-use.
Features
- Preserves fine nuclear details
- Good for immunostaining
- Contains no mercury
- Can be used with automated tissue processors
- Works well with standard and special stains because zinc is a stain enhancing mordant
| RT | 15675-01 | Formaldehyde/Zinc Fixative | 1 L | 21.00 | Add to Cart |
| RT | 15675-04 | Formaldehyde/Zinc Fixative | 4 L | 69.00 | Add to Cart |
Glyoxal 3% Fixative, Ready to Use
An alternative fixative to Formalin and Formaldehyde
EMS is proud to introduce an alternative to Formalin and Formaldehyde in many different applications.
Our unique kit comes premixed and measured and ready to use. The kit consists of 2 vials: Solution A & Solution B (Absolute Ethanol, Glyoxal Solution, Acetic Acid and water).
Instructions
Add solution A to solution B. Vortex the two solutions together and bring to a pH of 4.5 with the addition of NaOH 1M. The solution needs to be used immediately once mixed.
References
Sabatini DD. et al (1963) "The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation". J. Cell Biology. 17:19-58
Janigan DT. et al (1965) "The effects of aldehyde fixation on acid phosphatase activity in tissue blocks". J. Histochemistry. Cytochemistry. 13: 476-483
Dapson RW. et al (2006) "Glyoxal fixation and its relationship to immunocytochemistry." J. Histotechnology. 29: 65-76
Dapson RW. Et al (2007) "Glyoxal fixation: how it works and why it only occasionally needs antigen retrieval" lnforma healthcare, Dapson & Dapson, LLC
Richter K. et al (2018) "Glyoxal as an alternative to formaldehyde in immunostaining and super-resolution microscopy" The EMBO Journal. 37: 139-159
| 2-8°C | 16525 | Glyoxal 3% Fixative | 100 ml | 52.00 | Add to Cart |
Ampoules Breakers and Crackers
Please see the Sample Preparation section.
Perfusion Wash and Fixative Ready to Use
For all of the best results we offer ready to use Perfusion washes and fixatives which are recommended by the NeuroScience Association. All of our solutions are ready made and are of the highest quality.
We have three groups of solutions commercially available.
Group I
Perfusion wash and Fixative Technical Grade. To be used for Standard stains.
Perfusion Wash (1220SK): Phosphate Buffered Saline 01.M
Phosphate Buffer pH 7.2-7.4
Perfusion Fixative(1221SK): 10% Phosphate Buffered
Formaldehyde.
Group II
Perfusion wash and Fixative Reagent Grade. To be used for Standard and ImmunoHistochemistry Stains.
Perfusion Wash (1219SK): Phosphate Buffered Saline 0.1M
Phosphate Buffer pH 7.2-7.4.
Perfusion Fixative (1224SK): Paraformaldehye 4%/Sucrose
4% in Phosphate Buffer 0.1M Ph 7.2.
Group III
Perfusion wash and Fixative Superior Reagent Grade.
For the best results use this group. This is Sodium cacodylate based for NSA'S Disintegrative Degeneration Stain.
Perfusion Wash (1222SK): 0.8% NaCl/0.4% Dextrose/0.8% Sucrose/ 0.023% CaCl2 /Sodium.
Cacodylate 0.034%.
Perfusion Fixative (1223SK): 4% Paraformaldehyde/4% Sucrose/ 1.4% Sodium Cacodylate.
For a complete procedure and protocol for the use of these washes and fixatives please see the following site: www.neuroscienceassociates.com/perf-solution1 or 2 or 3.htm.
| 1220SK | Perfusion Wash, Technical | |
1000 ml | 34.00 | Add to Cart | |
| 1221SK | Perfusion Fixative, Technical | |
1000 ml | 48.00 | Add to Cart | |
| 1219SK | Perfusion Wash, Reagent | |
1000 ml | 32.00 | Add to Cart | |
| 1224SK | Perfusion Fixative, Reagent | |
1000 ml | 49.00 | Add to Cart | |
| 1222SK | Perfusion Wash, Super Reagent | |
1000 ml | 35.00 | Add to Cart | |
| 1223SK | Perfusion Fixative, Super Reagent | |
1000 ml | 52.00 | Add to Cart |
If you want a custom wash or fixative made please just let us know and we will be happy to make you whatever you require.
Technical Tip:
The Fixative for Use in Fixing Cornea
CoreDish™ Multiple Biopsy Containers Half Prefilled with 10% Formalin
Few recommendations concerning how the biopsies should be handled have been published. Performing a large number of biopsies means an increase in the number of containers handled and consequently a technical overload of the transmission network, which occurs without any financial counterpart. A new approach had to be developed in order to increase productivity.
We are proud to offer a multi-compartment container in the shape of a dish and half prefilled with 10% Neutral buffered Formalin, for holding and transporting biopsies. It is supplied with a leak-proof closure with O-ring ensuring total protection of contents. It conforms to OSHA directives. These CoreDisn™ measure only 15mm H x 95 mm Diameter. Each compartment is clearly identified to allow proper placement and visualization of the biopsy being inserted. Thanks to CoreDish™ it is no more necessary to use a multiple of individual containers, thereby reducing cost and risks of confusion.
CoreDish™ available in many configurations to serve in different biopsy, such as prostate, upper GI tract and lower GI tract. A label allows recording the related information, patient ID, doctor, date and time.
M970-D5B-2 CoreDish™ Breast Biopsy Container
Half Prefilled with 10% Formalin
This multi-compartment container (out of five compartments, four are labeled: LUQ, RUQ, LLQ, RLQ) in the shape of a dish and half prefilled with 10% Neutral Buffered Formalin, for holding and transporting biopsies. It is supplied with a leak-proof screw closure with O-ring ensuring total protection of contents. It conforms to OSHA directives. The dish measures only 15 x 95 mm in diameter. Each compartment is clearly identified to allow proper placement and visualization of the breast biopsy being inserted. A writing area for patient information is provided.
| RT | 15736-10 | Breast, 5 compartments | 10/pk | 63.00 | Add to Cart |
M970-D8P CoreDish™ Prostate Biopsy Container
Half Prefilled with 10% Formalin
For prostate biopsies. Eight compratments. Leakproof seal with O-ring lid. An area for patient information is provided. Seven labeled compartments: Base, Lateral Base, Medium, Lateral Medium, Apex, Lateral Apex.
| RT | 15736-20 | Prostate, 8 Compartments | 10/pk | 63.00 | Add to Cart |
M970-D8UGI CoreDish™ Upper GI Biopsy Container
Half Prefilled with 10% Formalin
For upper GI track biopsies. Eight compratments. Leakproof seal with O-ring lid. An area for patient information is provided. Seven labeled compartments: Gastric Card, Gastric Body, GE Junction, Gastric ATR, Distal Esophage, Pylorus, Duodeum.
| RT | 15736-30 | Upper GI, 8 Compartments | 10/pk | 63.00 | Add to Cart |
M970-D8LGI CoreDish™ Lower Gi Biopsy Container
Half Prefilled with 10% Formalin
For lower GI track biopsies. Twelve compratments. Leakproof seal with O-ring lid. An area for patient information is provided. Ten labeled compartments: Proximal Flexure Colon, Hepatic Fleure Colon, Distal Transverse Colon, Ascending Colon, Splenic flexure Colon, Cecum, Decending Colon, Terminal lleum, Rectum, Sigmmoid Colon.
| RT | 15736-40 | Lower GI, 12 Compartments | 10/pk | 54.00 | Add to Cart |
M970-D12P CoreDish™ Prostate Biopsy Container
Half Prefilled with 10% Formalin
For prostate biopsies. Twelve compratments. Leakproof seal with O-ring lid. An area for patient information is provided. Twelve labeled compartments: L Base, R Base, L Lateral Base, R Lateral Base, L Medium, R Medium, L Lateral Medium, R Lateral Medium, L Lateral, L Apex, R Apex, R Lateral Apex.
| RT | 15736-50 | Prostate, 12 Compartments | 10/pk | 63.00 | Add to Cart |
Glutaraldehyde
OCHCH2CH2CH2CHO F.W. 100.12 CAS #111-30-8
Specific Gravity @20/20°C: 1.131
EM Grade Distillation Purified
- The Universal Fixative for Electron Microscopy
- Purified to EM Grade from commercial grade by "all-glass vacuum-distillation"
- THE HIGHEST PURITY, free from polymers and other contaminants
- Each lot is tested and assayed in our lab before we ship and guarantee its purity
- Sealed under dry nitrogen
- Refrigerated prior to shipment
After glutaraldehyde has been purified to EM grade, it has a high risk of polymerizing if it is not handled properly. Electron Microscopy Sciences takes special precautions to assure a polymer-free, pure glutaraldehyde.
Every lot of our EM grade aqueous glutaraldehyde is assayed prior to shipping assuring you consistent purity and the highest possible quality. If stored properly, our Glutaraldehyde has a very long shelf life.
We package our aqueous glutaraldehyde in 10 ml, single dose ampoules, sealed under dry nitrogen, and kept refrigerated until it is shipped. We offer our glutaraldehyde in a wide range of percentages to simplify mixing procedures and avoiding waste.
EM grade aqueous glutaraldehyde, stored in volumes greater than 100 ml, with repeated defrosting and recapping, will polymerize in time, producing an absorption peak of 235nm rather than the 280nm of pure EM grade. The presence of polymerized glutaraldehyde greatly reduces its efficiency as a cross-linking agent, preventing proper fixation. For this reason, we provide 10 ml ampoules and 100 ml bottle quantities for larger applications.
8% Aqueous Solution
| 4 to (-18)°C | 16000 | 10 ml ampoule | 6.00 | Add to Cart | |
| 4 to (-18)°C | 16019 | Box 10 x 2ml | 23.00 | Add to Cart | |
| 4 to (-18)°C | 16020 | Box 10 x 10 ml ampoules | 27.00 | Add to Cart | |
| 4 to (-18)°C | 16021 | same as 16020 | 10 bxs | 260.00 | Add to Cart |
| 4 to (-18)°C | 16022 | same as 16020 | 20 bxs | 445.00 | Add to Cart |
| 4 to (-18)°C | 16010 | 100 ml bottle | 26.00 | Add to Cart |
10% Aqueous Solution
| 4 to (-18)°C | 16100 | 10 ml ampoule | 6.00 | Add to Cart | |
| 4 to (-18)°C | 16120 | Box 10 x 10 ml ampoules | 30.00 | Add to Cart | |
| 4 to (-18)°C | 16121 | same as 16120 | 10 bxs | 278.00 | Add to Cart |
| 4 to (-18)°C | 16122 | same as 16120 | 20 bxs | 475.00 | Add to Cart |
| 4 to (-18)°C | 16110 | 100 ml bottle | 26.00 | Add to Cart |
25% Aqueous Solution
| 4 to (-18)°C | 16200 | 10 ml ampoule | 7.00 | Add to Cart | |
| 4 to (-18)°C | 16220 | Box 10 x 10 ml ampoules | 33.00 | Add to Cart | |
| 4 to (-18)°C | 16221 | same as 16220 | 10 bxs | 315.00 | Add to Cart |
| 4 to (-18)°C | 16222 | same as 16220 | 20 bxs | 550.00 | Add to Cart |
| 4 to (-18)°C | 16210 | 100 ml bottle | 26.00 | Add to Cart |
50% Aqueous Solution
| 4 to (-18)°C | 16300 | 10 ml ampoule | 7.50 | Add to Cart | |
| 4 to (-18)°C | 16320 | Box 10 x 10 ml ampoules | 45.00 | Add to Cart | |
| 4 to (-18)°C | 16321 | same as 16320 | 10 bxs | 430.00 | Add to Cart |
| 4 to (-18)°C | 16322 | same as 16320 | 20 bxs | 780.00 | Add to Cart |
| 4 to (-18)°C | 16310 | 100 ml bottle | 44.00 | Add to Cart |
70% Aqueous Solution
| 4 to (-18)°C | 16350 | 10 ml ampoule | 8.50 | Add to Cart | |
| 4 to (-18)°C | 16360 | box 10 x 10 ml ampoules | 62.00 | Add to Cart | |
| 4 to (-18)°C | 16365 | box 10 x 2ml ampoules | 37.00 | Add to Cart |
Biological Grade
| 4 to (-18)°C | 16400 | 25% Solution | 450 ml | 20.00 | Add to Cart |
| 4 to (-18)°C | 16401 | 25% Solution | 4 x 450 ml | 76.00 | Add to Cart |
| 4 to (-18)°C | 16410 | 25% Solution | 1 gal. | 74.00 | Add to Cart |
| 4 to (-18)°C | 16411 | 25% Solution | 4 x 1 gal. | 269.00 | Add to Cart |
| 4 to (-18)°C | 16500 | 50% Solution | 450 ml | 23.00 | Add to Cart |
| 4 to (-18)°C | 16501 | 50% Solution | 4 x 450 ml | 80.00 | Add to Cart |
| 4 to (-18)°C | 16520 | 50% Solution | 1 liter | 43.00 | Add to Cart |
| 4 to (-18)°C | 16521 | 50% Solution | 4 x 1 liter | 154.00 | Add to Cart |
Serum Vial Aqueous Glutaraldehyde
Over the years, many of our valued customers have requested purchasing our aqueous Glutaraldehyde in serum vials. This packaging allows the user to puncture the special non-reactive seal with a hypodermic syringe and draw out the exact quantity of aqueous Glutaraldehyde needed without opening the container. This packaging assures freshness and purity of the aqueous Glutaraldehyde at all times.
| 4 to (-18)°C | 16216 | 25% EM Grade Aqueous Glutaraldehyde in Serum Vial | 10 x10 ml | 46.00 | Add to Cart |
| 4 to (-18)°C | 16214 | 25% EM Grade Aqueous Glutaraldehyde in Serum Vial | 100 ml | 38.00 | Add to Cart |
| 4 to (-18)°C | 16316 | 50% EM Grade Aqueous Glutaraldehyde in Serum Vial | 10 x10 ml | 58.00 | Add to Cart |
| 4 to (-18)°C | 16314 | 50% EM Grade Aqueous Glutaraldehyde in Serum Vial | 100 ml | 47.00 | Add to Cart |
* All other concentrations are available in serum vials upon request.
For any special concentration, or packaging requirements other than those already listed, please call us today. We will manufacture in accordance to your specifications.
Anhydrous EM Grade Glutaraldehyde:
Specially Prepared-10% Solution
Rapid-Freezing on a polished metal surface, at the temp. of liquid helium or liquid nitrogen, followed by freeze-substitution in osmium-acetone, has been shown to be a very useful technique for capturing rapid cellular events and for preserving cell ultrastructure.
* John M. Robinson and Morris J. Karnovsky, 1991. "Rapid-freezing Cytochemistry: Preservation of Tubular Lysosomes and Enzyme Activity". J. of Histochem., Cytochem. Vol. 39, No. 6, pp 787-792.
Ordering Information: (please call for additional information)
| 0-5°C | 16530 | 10% in Acetone | |
10 x 10 ml | 88.00 | Add to Cart |
| 0-5°C | 16531 | 10% in Ethanol | |
10 x 10 ml | 88.00 | Add to Cart |
| 0-5°C | 16532 | 10% in Methanol | |
10 x 10 ml | 88.00 | Add to Cart |
| 0-5°C | 16533 | 10% in Solvent of your choice | 10 x 10 ml | 88.00 | Add to Cart |
We would be pleased to fill your specific glutaraldehyde needs. Please call for prices on special concentrations or size requirements.
Glutaraldehyde, Pre-Made, Ready-to-Use, in Buffer
Glutaraldehyde 0.625% in 0.1M Phosphate Buffer, pH 7.4
| 0-5°C | 16535-05 | Glutaraldehyde 0.625% in Phosphate Buffer, pH 7.4 | 500 ml | 144.00 | Add to Cart |
| 0-5°C | 16535-10 | Glutaraldehyde 0.625% in Phosphate Buffer, pH 7.4 | 1 L | 272.00 | Add to Cart |
Glutaraldehyde 2% in 0.1M Phosphate Buffer, pH 7.4
| 0-5°C | 16536-05 | Glutaraldehyde 2% in Phosphate Buffer, pH 7.4 | 500 ml | 144.00 | Add to Cart |
| 0-5°C | 16536-10 | Glutaraldehyde 2% in Phosphate Buffer, pH 7.4 | 1 L | 280.00 | Add to Cart |
Glutaraldehyde 2% in 0.1M Sodium Cacodylate Buffer, pH 7.4
| 0-5°C | 16536-15 | Glutaraldehyde 2% in Sodium Cacodylate Buffer, pH 7.4 | 500 ml | 155.00 | Add to Cart |
| 0-5°C | 16536-20 | Glutaraldehyde 2% in Sodium Cacodylate Buffer, pH 7.4 | 1 L | 292.00 | Add to Cart |
Glutaraldehyde 2.5% in 0.1M Phosphate Buffer, pH 7.4
| 0-5°C | 16537-05 | Glutaraldehyde 2.5% in Phosphate Buffer, pH 7.4 | 500 ml | 162.00 | Add to Cart |
| 0-5°C | 16537-10 | Glutaraldehyde 2.5% in Phosphate Buffer, pH 7.4 | 1 L | 295.00 | Add to Cart |
Glutaraldehyde 2.5% in 0.1M Sodium Cacodylate Buffer, pH 7.4
| 0-5°C | 16537-15 | Glutaraldehyde 2.5% in Sodium Cacodylate Buffer, pH 7.4 | 500 ml | 161.00 | Add to Cart |
| 0-5°C | 16537-20 | Glutaraldehyde 2.5% in Sodium Cacodylate Buffer, pH 7.4 | 1 L | 300.00 | Add to Cart |
Glutaraldehyde 3% in 0.1M Phosphate Buffer, pH 7.4
| 0-5°C | 16538-05 | Glutaraldehyde 3% in Phosphate Buffer, pH 7.4 | 500 ml | 166.00 | Add to Cart |
| 0-5°C | 16538-10 | Glutaraldehyde 3% in Phosphate Buffer, pH 7.4 | 1 L | 300.00 | Add to Cart |
Glutaraldehyde 3% in 0.1M Sodium Cacodylate Buffer, pH 7.4
| 0-5°C | 16538-15 | Glutaraldehyde 3% in Sodium Cacodylate Buffer, pH 7.4 | 500 ml | 168.00 | Add to Cart |
| 0-5°C | 16538-20 | Glutaraldehyde 3% in Sodium Cacodylate Buffer, pH 7.4 | 1 L | 305.00 | Add to Cart |
Glutaraldehyde 3% Sodium Phosphate Buffer, pH 7.2
| 0-5°C | 16539-45 | Glutaraldehyde 3% in Sodium Phosphate Buffer, pH 7.2 | 10 x 10 ml | 39.00 | Add to Cart |
| 0-5°C | 16539-50 | Glutaraldehyde 3% in Sodium Phosphate Buffer, pH 7.2 | 500 ml | 139.00 | Add to Cart |
| 0-5°C | 16539-55 | Glutaraldehyde 3% in Sodium Phosphate Buffer, pH 7.2 | 1 L | 282.00 | Add to Cart |
Glutaraldehyde 4% in 0.1m Phosphate Buffer, pH 7.3
| 0-5°C | 16539-60 | Glutaraldehyde 4% in 0.1m Phosphate Buffer, pH 7.3 | 10 x 10 ml | 41.00 | Add to Cart |
| 0-5°C | 16539-65 | Glutaraldehyde 4% in 0.1m Phosphate Buffer, pH 7.3 | 500 ml | 139.00 | Add to Cart |
| 0-5°C | 16539-70 | Glutaraldehyde 4% in 0.1m Phosphate Buffer, pH 7.3 | 1 L | 278.00 | Add to Cart |
Glutaraldehyde 2% Paraformaldehyde 2% in Phosphate Buffer, pH 7.4
| 0-5°C | 16536-06 | Glutaraldehyde 2% Paraformaldehyde 2% in Phosphate Buffer, pH 7.4 | 100 ml | 40.00 | Add to Cart |
| 0-5°C | 16536-07 | Glutaraldehyde 2% Paraformaldehyde 2% in Phosphate Buffer, pH 7.4 | 500 ml | 159.00 | Add to Cart |
| 0-5°C | 16536-08 | Glutaraldehyde 2% Paraformaldehyde 2% in Phosphate Buffer, pH 7.4 | 1 Liter | 286.00 | Add to Cart |
Glutaraldehyde 2.5% Paraformaldehyde 1% in Phosphate Buffer, pH 7.4
| 0-5°C | 16537-06 | Glutaraldehyde 2.5% Paraformaldehyde 1% in Phosphate Buffer, pH 7.4 | 100 ml | 38.00 | Add to Cart |
| 0-5°C | 16537-07 | Glutaraldehyde 2.5% Paraformaldehyde 1% in Phosphate Buffer, pH 7.4 | 500 ml | 158.00 | Add to Cart |
| 0-5°C | 16537-08 | Glutaraldehyde 2.5% Paraformaldehyde 1% in Phosphate Buffer, pH 7.4 | 1 Liter | 284.00 | Add to Cart |
Glutaraldehyde 2.5% Aqueous
| 0-5°C | 16537-16 | Glutaraldehyde 2.5% Aqueous | 10 x 10 ml | 26.00 | Add to Cart |
| 0-5°C | 16537-17 | Glutaraldehyde 2.5% Aqueous | 500 ml | 68.00 | Add to Cart |
| 0-5°C | 16537-18 | Glutaraldehyde 2.5% Aqueous | 1 Liter | 118.00 | Add to Cart |
Glutaraldehyde 3.0% Aqueous
| 0-5°C | 16537-19 | Glutaraldehyde 3% Aqueous | 100 ml | 28.00 | Add to Cart |
| 0-5°C | 16537-20-S | Glutaraldehyde 3% Aqueous | 500 ml | 68.00 | Add to Cart |
| 0-5°C | 16537-21 | Glutaraldehyde 3% Aqueous | 1 Liter | 118.00 | Add to Cart |
Glutaraldehyde 3.0% Paraformaldehyde 3% in 0.1M Phosphate Buffer, pH 7.4
| 0-5°C | 16538-06 | Glutaraldehyde 3% Paraformaldehyde 3% in Phosphate Buffer, pH 7.4 | 10 x 10 ml | 28.00 | Add to Cart |
| 0-5°C | 16538-07 | Glutaraldehyde 3% Paraformaldehyde 3% in Phosphate Buffer, pH 7.4 | 500 ml | 68.00 | Add to Cart |
| 0-5°C | 16538-08 | Glutaraldehyde 3% Paraformaldehyde 3% in Phosphate Buffer, pH 7.4 | 1 Liter | 98.00 | Add to Cart |
Glutaraldehyde 4% in 0.1M in Sodium Cacodylate Buffer, pH 7.4
| 0-5°C | 16539-06 | Glutaraldehyde 4% in 0.1M Sodium Cacodylate Buffer, pH 7.4 | 10 x 10 ml | 40.00 | Add to Cart |
| 0-5°C | 16539-07 | Glutaraldehyde 4% in 0.1M Sodium Cacodylate Buffer, pH 7.4 | 500 ml | 140.00 | Add to Cart |
| 0-5°C | 16539-08 | Glutaraldehyde 4% in 0.1M Sodium Cacodylate Buffer, pH 7.4 | 1 Liter | 280.00 | Add to Cart |
Glutaraldehyde 5% Aqueous
| 0-5°C | 16539-15 | Glutaraldehyde 5% Aqueous | 10 x 10 ml | 27.00 | Add to Cart |
| 0-5°C | 16539-20 | Glutaraldehyde 5% Aqueous | 500 ml | 68.00 | Add to Cart |
| 0-5°C | 16539-25 | Glutaraldehyde 5% Aqueous | 1 Liter | 118.00 | Add to Cart |
Glutaraldehyde 5% in 0.1M Phosphate Buffer, pH 7.4
| 0-5°C | 16539-30 | Glutaraldehyde 5% in 0.1M Phosphate Buffer, pH 7.4 | 10 x 10 ml | 40.00 | Add to Cart |
| 0-5°C | 16539-35 | Glutaraldehyde 5% in 0.1M Phosphate Buffer, pH 7.4 | 500 ml | 139.00 | Add to Cart |
| 0-5°C | 16539-40 | Glutaraldehyde 5% in 0.1M Phosphate Buffer, pH 7.4 | 1 Liter | 279.00 | Add to Cart |
Glutaraldehyde/Formaldehyde
Fixative (Karnovsky's)
This fixative contains 3% glutaraldehyde and 2% formaldehyde in 0.1M phosphate buffer, pH 7.4.
Karnovsky's with formalin 2% and Biological glutaraldehyde 3% in 0.1m Phosphate Buffer.
| 0-5°C | 15731-10 | Karnovsky Fixative Reagent Grade | 1 L | 260.00 | Add to Cart |
Karnovsky's with paraformaldehyde solution and EM grade glutaraldehyde.
Please Note: This kit can be formulated to fit your requirements. Please contact us.
| RT | 15732-10 | Karnovsky Fixative EM Grade | 1 L | 286.00 | Add to Cart |
Ampoule Breaker; Soft Silicone
Reusable – Made from soft silicone. It offers a simple way to break pre-scored glass ampoules safely. Accommodates 5, 7, and 10 ml size ampoules. Reduces injury and spillage.
| 60600 | Ampoule Breaker | each | 16.00 | Add to Cart |
Ampoule Cracker™
The Ampoule Cracker™ is a disposable plastic fracture and safety collar, which is specially designed to aid in the breaking open of pre-scored glass ampoules. Simply slip the Ampoule Cracker™ over the top at the pre-scored portion, snap it, and discard both the ampoule top and the safety collar to prevent cross contamination.
| 60605-04 | Ampoule Cracker for Ampoule Size 1-4 ml | 500/pk | 41.00 | Add to Cart |
| 60605-10 | Ampoule Cracker for Ampoule Size 5-10 ml | 500/pk | 41.00 | Add to Cart |
| 60605-20 | Ampoule Cracker for Ampoule Size 10-20 ml | 500/pk | 77.00 | Add to Cart |
Break-Safe™ Ampoule Opener
EMS Introduces Break-Safe™ allowing you to safely open up to 3 ampoules at one time.
Box style design allows one handed opening of up to 3 ampoules at a time. Gently pressing down on the lid snaps ampoules necks clean while keeping them contained within the device and away from fingers. After removing ampoules contents, simply turn the device upside down over a trash receptacle to safely discard ampoules and tops.
Features
- Includes 4 inserts to allow opening of 1ml, 2ml, 5ml and 10 ml ampoules.
- Open ampoules easily and safely
- Transparency allows unobstructed viewing of ampoules contents at all times.
- Copolyester plastic construction has broad chemical resistance
| 60607 | Break-Safe™ Ampoule Opener | each | 47.00 | Add to Cart |
HISTOCHOICE™MB®* – The Molecular Biology Fixative
- SAFE – Contains no Formaldehyde, Glutaraldehyde or Mercury
- NON-TOXIC – Is completely odorless and can be safely disposed
of down the drain
HISTOCHOICE™ MB® Tissue Fixative is the first fixative designed for the molecular biologist. It is specialty formulated to preserve antigenic sites for antibody probes and nucleic acid sites for immunohistochemistry. HISTOCHOICE™MB® replaces formaldehyde based, alcohol based, Zenkers, B-5, B-3, Bouin's and other fixatives with superior results. Tissue fixed in HISTOCHOICE™MB® exhibit vibrant staining, better nuclear and cytoplasmic detail, and will retain a crisp appearance even after long-term fixation.
Because this formula is designed specially for molecular biology applications, HISTOCHOICE™MB® fixed sections do not require pre-digestion or other recovery procedures to make important sites available.This means you spend less time preparing slides and more time doing research.
More than a fixative, HISTOCHOICE™MB® is a preservative that leaves antigens and nucleic acids in their native state, allowing binding for specific probes. Primary antibodies can often be diluted several-fold due to the increased number of preserved antigenic sites, saving cost on every slide you process.
HISTOCHOICE™MB® fixed tissue retains a much more natural look and feel than formalin fixed tissue. It is important to remember that these tissues are not fixed by formalin, but rather preserved by HISTOCHOICE™MB®.
HISTOCHOICE™MB® is on long-term backorder... please check back for updates.
| RT | 64115-01 | HISTOCHOICE™MB® Fixative | 1 L | 60.00 | Add to Cart |
| RT | 64115-04 | HISTOCHOICE™MB® Fixative | 4 L | 190.00 | Add to Cart |
| RT | 64115-44 | HISTOCHOICE™MB® Fixative | 4 x 4 L | 695.00 | Add to Cart |
* HISTOCHOICE is registered name of Amresco
Hollande's Fixative
A modification of Bouin's solution. It is stable and will decalcify small bone specimens. Tissue that is fixed with Hollande's can be stained successfully with most stains, and the cupric acetate in the solution stabilizes red blood cell membranes and cosinophil and endocrine cell granules so that less lysis occurs than with Bouin's solution. Hollande's is widely used as a fixative for biopsy specimens of the gastrointestinal tract.
Thorough washing of the fixative prior to placing the specimen in a phosphate buffered formalin solution is necessary because the salts present in the solution will form an insoluble phosphate precipitate. Packaged in leak-proof histo-containers; prefilled ready-to-use.
| Catalog # | Cup Size | Filled | Pack/cs | Price | ||
| RT | 64120-10 | 15 ml | 10 ml | 50 | 78.00 | Add to Cart |
| RT | 64120-20 | 30 ml | 20 ml | 50 | 98.00 | Add to Cart |
| RT | 64120-30 | 60 ml | 30 ml | 20 | 46.00 | Add to Cart |
Bulk packaging is available
| RT | 64120-01 | Hollande's Fixative | 1 L | 46.00 | Add to Cart |
| RT | 64120-04 | Hollande's Fixative | 4 L | 168.00 | Add to Cart |
McDowell Trumps Fixative
| RT | 18030-05 | McDowell Trumps Fixative | 500 ml | 33.00 | Add to Cart |
| RT | 18030-10 | McDowell Trumps Fixative | 1 L | 39.00 | Add to Cart |
Michel's Fixative, pH 7.0-7.4
| RT | 18040-05 | Michel's Fixative | 500 ml | 50.00 | Add to Cart |
| RT | 18040-10 | Michel's Fixative | 1 L | 85.00 | Add to Cart |
| RT | 18040-20 | Michel's Fixative Pre-filled | 20 x 10 ml | 75.00 | Add to Cart |
Technical Tip:
Preparation of Tissue Biopsy in Michel's Fixative.
Michel's Transport Medium
Michel's Transport Medium allows for the shipping of specimens for further immunofluorescence work. It preserves morphology and antigenicity during transport. It is not a buffer or fixative. It is simply a vehicle to transport samples. The solution must be stored at refrigerated temperatures. Do not freeze. Samples may be stored up to 5 days in this medium at room temperature.
Features
- Comes in 250ml, 500ml, 1 Liter, Gallon, or 15ml fill in SecurTainer™
- Can be used with Michel's Buffer (washing solution)
| 4-8°C | 18042-05 | Michel's Transport Medium | 250 ml | 28.00 | Add to Cart |
| 4-8°C | 18042-10 | Michel's Transport Medium | 500 ml | 48.00 | Add to Cart |
| 4-8°C | 18042-15 | Michel's Transport Medium | 1 L | 65.00 | Add to Cart |
| 4-8°C | 18042-16 | Michel's Transport Medium | gal | 142.00 | Add to Cart |
| 4-8°C | 18042-17 | Michel's Transport Medium, 15ml fill in SecurTainer™ | 50/cs | 80.00 | Add to Cart |
Mirsky's Fixative™
- Saponin/Glyoxylate Fixative
- Preserves Immunohistological activity
- Contains no formaldehyde
- Fast-acting
Mirsky's Fixative™ is a superior fixing agent for use in immunohistological and immunological protocols. Mirsky's contains no formaldehyde or glutaraldehyde. Therefore, it has considerably reduced toxicity and virtually no odor. Mirsky's Fixative is neutral, buffered, and isotonic (308 mOsmol). Additionally, Mirsky's Fixative does not contain toxic or hazardous buffers such as cacodylate or barbital. Hardening or shrinkage of tissue is considerably reduced.
Double reactive sites afford excellent cross-linking properties while maintaining sample enzyme activity. Samples processed in Mirsky's Fixative for light microscopy can subsequently be used in electron microscopy procedures. Special buffer systems may be used in place of the buffer provided.
Use as replacement for formalin and/or glutaraldehyde fixatives in immunohistological and immnocytological staining protocols. Tissue size is unlimited, as long as the sample is no thicker than 0.5cm in at least one plane to assure uniformity of tissue penetration. The gross visual appearance will be different than in formaldehyde and tissue may seem "raw". This is due to double site binding of the fixative with a resultant reduction in tissue shrinkage. Microscopic examination of tissue morphology will be noticeably improved. This material is intended to maintain enzyme and antibody activity. For best results, tissue section of high digestive enzyme content should be thoroughly rinsed in saline solution before fixation (e.g. trypsin in intestinal samples). Once fixed, tissue may be retained in Mirsky's Fixative and ethanol indefinitely.
Mirsky's Fixative is normally distributed as a concentrated two bottle system, although high through-put laboratories often use the single bottle ready-to-use format. The two bottle system is comprised of Mirsky's Fixative 10X Concentrated and Mirsky's Fixative 10X Buffer. To reconstitute to working strength, add 1 part Mirsky's Fixative 10X Buffer to 8 parts distilled or deionized water, mix thoroughly, then add 1 part Mirsky's Fixative 10X Concentrate and mix again.
The two bottle system has a shelf life of 12 months while the ready-to-use format has a shel life of 30 days.
| RT | 15560 | Mirsky's Fixative System | |
200 ml | 42.00 | Add to Cart |
| RT | 15561-05 | Mirsky's Fixative Ready-to-Use in Ethanol | |
500 ml | 24.00 | Add to Cart |
| RT | 15561-10 | Mirsky's Fixative Ready-to-Use in Methanol | 1L | 42.00 | Add to Cart | |
| RT | 15561-20 | Mirsky's Fixative Ready-to-Use in Solvent of your choice | 2L | 82.00 | Add to Cart |
Russell's Zenker Fixative (Russel's Modified Zenker)
Due to the hazardous nature of mercury, Zenker's fluid is no longer available. In place we are offering Russell's Zenker Fixative, which contains Zinc Chloride. The staining results produced by this fixative are comparable to those of Zenker's Stock solution. Russell's Modified Zenker's is used in the same manner as Zenker's stock solution.
| RT | 64123-05 | Russell's Zenker Fixative | 500 ml | 27.00 | Add to Cart |
| RT | 64123-06 | Russell's Zenker Fixative | 1 L | 46.00 | Add to Cart |
Formic Acid 96%, Reagent, A.C.S.
HCOOH F.W. 46.03 CAS #64-18-6
Specific Gravity: 1.22
| RT | 15750 | Formic Acid, 96% | 225 ml | 18.00 | Add to Cart |
| RT | 15760 | Formic Acid, 96% | 1 liter | 48.00 | Add to Cart |
Gold
Chloride, Hydrate(Chloroauric Acid; Hydrogen Tetrachloroaurate)
H(AuCl4)·H2O F.W. 357.79 CAS #16961-25-4
Assay 99.9985%
Gold contents 49%+
A highly purified gold compound for the preparation of Colloidal Gold sols. Preweighed to facilitate your work. (Preparation instructions for Gold Sols included).
| RT | 16580 | 10 x 1/10g | 156.00 | Add to Cart | |
| RT | 16582 | 10 x ½g | 625.00 | Add to Cart | |
| RT | 16583 | 1g | 140.00 | Add to Cart | |
| RT | 16584 | 10x1g | 1,195.00 | Add to Cart |
Gold Chloride Aqueous Solutions
| RT | 16586 | Gold Chloride, 0.1% Aqueous | |
500 ml | 72.00 | Add to Cart |
| RT | 16587 | Gold Chloride, 0.2% Aqueous | |
500 ml | 112.00 | Add to Cart |
| RT | 16588 | Gold Chloride, 0.5% Aqueous | |
500 ml | 226.00 | Add to Cart |
| RT | 16589 | Gold Chloride, 1.0% Aqueous | |
500 ml | 506.00 | Add to Cart |
| RT | 16590 | Gold Chloride, 10.0% Aqueous | |
500 ml | 3,995.00 | Add to Cart |
Gold, Immuno, see Section 2 ImmunoGold Reagents
Gum Mastic
| 0-4°C | 16590-01 | Gum Mastic, 2.5% in Alcohol | |
500 ml | 111.00 | Add to Cart |
| 0-4°C | 16591 | Gum Mastic, 5% in Alcohol | |
500 ml | 116.00 | Add to Cart |
| 0-4°C | 16592 | Gum Mastic, 10% in Alcohol | |
500 ml | 143.00 | Add to Cart |
| 0-4°C | 16595 | Gum Mastic, 10% in Isopropanol | 500 ml | 143.00 | Add to Cart |
Hexamethylenetetramine, A.C.S. Reagent, Granular
(Urotropine; Methenamine)
C6N4 F.W. 140.19 CAS #100-97-0
| Assay: | 99.0-100.5% |
Specifications
| Residue after Ignition | 0.1% | |
| Chloride | 0.014% | |
| Sulfate | To Pass Test | |
| Heavy Metals. | 0.001% |
Used in silver stains.
Swift, J.A. (1968) Microsc. Soc. 88, 449.
| RT | 16710 | 50g | 14.00 | Add to Cart | |
| RT | 16710-25* | 25g | 8.50 | Add to Cart | |
| RT | 16712 | Methynamine, 3% Aqueous | 500 ml | 25.00 | Add to Cart |
Hexylene Glycol, Reagent, A.C.S.
(2-Methyl-2,4-pentanediol)
(CH3)2C(OH)CH2CH(OH)CH3 F.W. 118.18 CAS #107-41-5
Specific Gravity @25°C 0.923
A.C.S. Specifications
| Assay | 99% | |
| IR Spectrum | To Pass Test | |
| Refractive Index @20°C | 0.921-0.923 |
Mixed with water in all proportions. Used as a dehydrating agent for fat tissues.
| RT | 16740 | 450 ml | 25.00 | Add to Cart | |
| RT | 16750 | 4 x 450 ml | 92.00 | Add to Cart |
