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Electron Microscopy Sciences

Illuminators, Magnifiers, Microscopes, & Graticles

arrow13NIGHTSEA™ Fluorescence Viewing Systems

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Image GalleryEclipse MicroTentarrow12Eclipse MicroTent
  • Tent
  • Tent
  • Tent

The patented (US Pat. No. 10,175,467) Eclipse MicroTent™ is a unique product for fluorescence microscopy that provides local darkness around conventional laboratory stereo microscopes. Fluorescence microscopes are kept in dark rooms for good reason - fluorescence can be weak and in many cases it can be difficult to see well if there is any ambient light. Microscopes may be on lab benches in shared spaces, near windows, or in other difficult-to-darken locations such as in the field. Turning off overhead lights can help but inconveniences others.

The Eclipse Microtent™ creates local darkness around a microscope while still providing easy access to the sample stage and the focus and zoom controls. It is designed for stereo microscopes but could potentially be used with many varieties of compound microscopes.

Features

  • Opening for the microscope oculars with elastic sleeve to minimize light entry
  • Large front flap provides easy access to the sample stage and can fasten open
  • Arm slots on sides to provide access to focus and zoom controls
  • User-customizable feedthrough patches to provide additional penetrations for camera port, power cords, CO2 lines, or other features as you need
  • Tru-Block™ Eye Shields included with every Eclipse MicroTent
  • Folds flat for storage
  • Dimensions: 46 x 30 x 50 cm (18 x 12 x 20 in.)

Please contact us if you require a custom-sized tent.

Ordering Information

SFA-TENT Eclipse MicroTent each 325.00 Add to Cart
Technical Data SheetEye Shieldsarrow12Eye Shields

Light entering your eyes from the side can interfere with what you want to see in microscopy in general, and fluorescence microscopy in particular. Eye cups are available, but the standard ones don't extend far from the microscope and don't do a good job as ambient light increases. Our soft, molded rubber high-sided microscope eye shields are the answer. The tall wings extend up far enough to truly shield your eyes from any level of ambient light and eliminate distractions so that you can see your subject better. Two pairs (one Standard, one Compact) will be included with every Eclipse MicroTent™, and you can also purchase them separately.

The Tru-Block Eye Shields are available in two sizes

  • Standard - fits 36 - 45mm (1.45 - 1.75")
  • Compact - fits 28 - 37mm (1.10 - 1.46")

Ordering Information

SFA-EYE-S Tru-Block Eye Shields - Standard set 18.00 Add to Cart
SFA-EYE-C Tru-Block Eye Shields - Compact set 18.00 Add to Cart
Modular White Light Headarrow12Modular White Light Head

This is an extremely convenient option for general illumination and as a focusing aid for fluorescence imaging. Now if you are using the NIGHTSEA system for fluorescence you do not need a separate white light source. Just exchange the fluorescence excitation light head module for the white-light module in a matter of seconds.

Ordering Information

SFA-LH-WH Modular White Light Head each 230.00 Add to Cart
Modular Infrared Light Head
850nm IR Light Head
Modular Infrared Light Head
850nm IR Light Head on SFA lamp base
arrow12Modular Infrared Light Head

Researchers sometimes need an infrared light source for non-intrusive observation of behavior. And sometimes the subjects are small and you need to make the observations with a stereo microscope.

The SFA-LH-IR850 is an 850nm light source that is compatible with the NIGHTSEA Model SFA Stereo Microscope Fluorescence Adapter. The light head plugs directly into the lamp base supplied with the SFA system. You can acquire the light head as a supplement to the SFA system that you already own for fluorescence observation, or you can acquire it in combination with a lamp base as a stand-alone device.

The light head incorporates a high power 850nm LED and a medium beamwidth diffusing lens to create a smooth illumination area. The output is centered at 850nm with a FWHM (full width at half maximum) of approximately 50nm. There is virtually no emission at wavelengths shorter than 750nm.

  • Conventional camera under normal room light
  • Test target for IR Light Head, Infrared

  • Click to enlarge

The first image to the right shows a test target with circles of 2, 5, and 10cm diameter. This photograph was taken with a conventional camera under normal room light, and with the IR light head illuminated at full power, directed at the target center from a distance of approximately 10cm (4 in.). The illumination spot is not visible either to this camera or to the naked eye. The secind image to the right is a photograph made under the same lighting conditions, but with a camera that had been modified to image only infrared light. We recommend that you use the DIM base with the LH-IR850 in order to have finer control of illumination intensity.

Please note that infrared light is not visible to the human eye and to many cameras. You will need an appropriate camera to record the observations.

Ordering Information

SFA-LH-IR850 Modular Infrared Light Head each 250.00 Add to Cart
SYS-IR850 Modular Infrared Light Head w/DIM Base each 915.00 Add to Cart
Battery and Chargerarrow12Battery and Charger

Compact battery pack that can run the Stereo Microscope Fluorescence Adapter (SFA) all day long. The battery enables truly portable operation so that you can set up demos anywhere or explore fluorescence in the field, without having to worry about plugging into the power grid.

Just plug the battery into the SFA base instead of the usual power connector. The battery has been tested at over 8 hours of continuous operation, and it will last even longer if you turn the system off when you don't need it. Plug it into the included charger overnight to recharge.

Specifications

Battery Type Nickel Metal Hydride (NiMH)
Capacity 12V, 3.8 Ah
Dimensions 11.4 x 8.9 x 5.6 cm (4.5 x 3.5x 2.19 in)
Weight 0.7 kg (1 lb 9 oz)
Operation Duration 8 hours continuous
Charge time Overnight
Charger 50/60 Hz, 110/220V (US type plug)

SFA-BATT Battery and Charger each 280.00 Add to Cart


SFA + Eclipse MicroTent + Battery = Fluorescence Everywhere

Combine the battery with the Eclipse MicroTent and you not only don't need a place to plug in, you don't even need to be in the dark! We have used this combination to do fluorescence microscopy at a beach in the middle of the day, in the desert at high noon, and more. Fluorescence can be found everywhere, and now you have the tools to go there.

arrow12SFA A LA CARTE

Need an extra, not a set? Order from here

arrow12SFA Light Heads
SFA-LH-UV Light Head, Ultraviolet each 400.00 Add to Cart
SFA-LH-VI Light Head, Violet each 250.00 Add to Cart
SFA-LH-RB Light Head, Royal Blue each 250.00 Add to Cart
SFA-LH-CY Light Head, Cyan each 250.00 Add to Cart
SFA-LH-GR Light Head, Green each 250.00 Add to Cart
arrow12SFA Barrier Filters
SFA-BF-UV Barrier Filter, Ultraviolet each 208.00 Add to Cart
SFA-BF-VI Barrier Filter, Violet each 208.00 Add to Cart
SFA-BF-RB Barrier Filter, Royal Blue each 208.00 Add to Cart
SFA-BF-RB-GO Barrier Filter, Green Only each 208.00 Add to Cart
SFA-BF-CY Barrier Filter, Cyan each 208.00 Add to Cart
SFA-BF-GR Barrier Filter, Green each 208.00 Add to Cart
arrow12SFA Filter Shields
SFA-SH-UV Filter Shield, Ultraviolet each 105.00 Add to Cart
SFA-SH-VI Filter Shield, Violet each 105.00 Add to Cart
SFA-SH-RB Filter Shield, Royal Blue each 105.00 Add to Cart
SFA-SH-RB-GO Filter Shield, Green Only each 105.00 Add to Cart
SFA-SH-CY Filter Shield, Cyan each 105.00 Add to Cart
SFA-SH-GR Filter Shield, Green each 105.00 Add to Cart
arrow12SFA Adapters
SFA-AD Adapter each 168.00 Add to Cart
SFAZ-AD Leica EZ4 Adapter each 168.00 Add to Cart
SFA-XL-AD Oversize Adapter each 168.00 Add to Cart
arrow12SFA Bases
SFA-BASE Standard Base each 415.00 Add to Cart
SFA-DIM-N DIM Base each 665.00 Add to Cart
SFA-PULSE-N PULSE Base each 665.00 Add to Cart
Light Head Hangers, long and short working distanceSFA Dual Light Head CableSFA Single Light Head Cable, BNCarrow12Light Head Hangers and Cables
  • Light Head Hanger, long working distance
  • Light Head Hanger, long working distance, highest position
  • Light Head Hanger, long working distance, lowest position
  • Light Head Hanger, short working distance
  • Light Head Hanger, short working distance, highest position
  • Short Arm Light Head Hanger, lowest position
  • Single Light Head Cable, BNC
  • Dual Light Head Cable, BNC

The NIGHTSEA light head hangers and cables conveniently hold the light head in lieu of the gooseneck lamp. The hangers mount to the end of any of the thumbscrews on the SFA adapter ring. They are available with two different pivot arm lengths for use with long and short working distance microscopes.

The cables are available in single and dual styles. With the single cable and one light head hanger you can remove the light head from the gooseneck and mount it on the SFA adapter ring. With the dual cable you can power TWO light heads from one base. Just add a second light head and two light head hangers and you have double the excitation intensity.

The light head hangers are offered for microscopes with different working distances below the objectives

  • Long working distance (LWD) – 6.5 cm (2.56 in.) and greater
  • Short working distance (SWD) – 6.3-4.6 cm (2.48-1.81 in.)

The light head hangers and cables are available individually or in combination kits.

Ordering Information

SFA-LHH-L Light Head Hanger, long working distance each 95.00 Add to Cart
SFA-LHH-S Light Head Hanger, short working distance each 95.00 Add to Cart
SFA-LHC Single Light Head Cable each 30.00 Add to Cart
SFA-DLHC Dual Light Head Cable each 65.00 Add to Cart
SFA-HK-L Single hanger kit, long working distance, SFA-LHC + SFA-LHH-L kit 125.00 Add to Cart
SFA-HK-S Single hanger kit, short working distance, SFA-LHC + SFA-LHH-S kit 125.00 Add to Cart
SFA-DHK-L Dual hanger kit, long working distance, SFA-DLHC + 2x SFA-LHH-L kit 245.00 Add to Cart
SFA-DHK-S Dual hanger kit, short working distance, SFA-DLHC + 2x SFA-LHH-S kit 245.00 Add to Cart
arrow12Mobile Extension Kit
  • Hand grip with light head enclosure; extension cable; and barrier filter glasses
  • Hand grip with Royal Blue light head installed and connected to the power cable, and the Royal Blue filter glasses
  • Inspecting artwork with the SFA Mobile Extension Kit

The Mobile Extension Kit provides added versatility to your SFA microscope fluorescence adapter system by enabling you to use the light head as a handheld light source. Do you work with both macro and micro subjects? Do you want to screen larger specimens before dissection or other microscope work? Can't fit everything under the microscope? Then the SFA-MEK Mobile Extension Kit is a way to get more use out of your system.

The kit includes three components

  • Rubber-coated hand grip with wrist lanyard and custom made enclosure that holds an SFA Light Head securely. The slots on the side of the enclosure provide for cooling and also let you see the color code of an installed light head.
  • Lightweight extension cable.
  • Barrier filter glasses.

To use the kit just take the light head off the SFA gooseneck base, connect it to one end of the extension cable, and attach the other end of the cable to the gooseneck. Insert the light head in the holder and screw down the thumbscrew to secure it in place. Put on the filter glasses that correspond to the light head color and you're ready to go! If you want to use the Mobile Extender with more than one excitation wavelength you can add additional barrier filter glasses at any time.

If you want to use the Mobile Extension Kit with the SFA system you already own, then the SFA-MEK is all you need.

If you want to add additional excitation/emission combinations to use only for handheld operation and not for your microscope, you would need a Light Head plus a pair of Barrier Filter Glasses for each color.

Ordering Information

SFA-MEK Mobile Extension Kit each 225.00 Add to Cart
Technical Data SheetSwitch Box Kitarrow12Switch Box Kit

Easily change the lighting conditions while you work with the Switch Box Kit.

Kit consists of a BNC switch box, 3 BNC cables (including one with a female to female adapter to attach to the gooseneck), and 2 Light Head Hangers.

Ordering Information

SFA-SWK-S Switch Box Kit, Short working distance: BNC switch box, 3 BNC cables, one with female/female gooseneck adapter, 2 LH Hangers each 350.00 Add to Cart
SFA-SWK-L Switch Box Kit, Long working distance: BNC switch box, 3 BNC cables, one with female/female gooseneck adapter, 2 LH Hangers each 350.00 Add to Cart
arrow11Lamp Base Light Control Options

The SFA lamp base is available in three versions: Standard, DIM, and PULSE. Standard lamp bases provide a steady light when turned on. DIM lamp bases feature an OFF/ON/DIM switch to change the intensity of the light. PULSE lamp bases incorporate a BNC connector that accepts a voltage signal to control the excitation source ON/OFF.

Only one control option is available per base. This DIM and PULSE options can be purchased as part of a new system, as a retrofit to an existing base, or in a stand-alone lamp base purchased a la carte.

Dimmer switch option on SFA

Target at max and min excitation

arrow12NIGHTSEA DIM Option

The DIM option adds a combined switch and dimmer control.

When you first turn the unit on it is at full power.

As you rotate the switch the intensity decreases, reaching about 30% power at the minimum setting.

You have finer control in the brighter portion of the adjustment range.

An intensity indicator (1-10) makes it easy to record and repeat preferred settings.

Ordering Information

SFA-DIM-R Dim Option - Retrofit each 275.00 Add to Cart
BNC input connector for external controlBNC input connector for external control

arrow12NIGHTSEA PULSE Option

The PULSE option adds a BNC connector on the rear of the base that accepts a user-supplied voltage input to turn the light on and off. Some possible applications

  • External control (computer, function generator, etc.) for precise illumination timing for photoactivation, behavioral experiments
  • PWM input for light dimming
  • Footswitch for hands-free operation - contact EMS for details

Specifications

  • Voltage input - 2.8 - 6.0 VDC for ON, <0.6VDC OFF
  • Maximum operation frequency - 10kHz
  • BNC cable not included

Ordering Information

SFA-PULSE-R PULSE Option - Retrofit each 275.00 Add to Cart
Training ManualNIGHTSEA DFP-1 Dual Fluorescent Protein Flashlightarrow12NIGHTSEA™ DFP™ Dual Fluorescent Protein Excitation Flashlights

Rapid screening of your fluorescent transgenic experiments

  • Dual Fluorescent Protein Flashlight
  • Dual Fluorescent Protein Flashlight

The Model DFP Dual Fluorescent Protein Flashlights include two different, switch-selectable high intensity LEDs for excitation of fluorescent proteins. For years the most popular fluorescent colors have been green (GFP, eGFP, fluorescein, etc.) and red (DsRed, TdTomato, etc.), and we offered only one version of the DFP, with a combination of blue and green LEDs for exciting these fluorophores. This is still our 'standard' DFP product, but we are now offering build-to-order combinations using any two of the excitation colors that are available with our popular Model SFA Stereo Microscope Fluorescence Adapter.

With every DFP light you will receive two pairs of barrier filter glasses and a convenient storage case. The glasses are well matched to the excitation so that they block the reflected excitation light while transmitting the fluorescence with high efficiency, providing excellent viewing contrast.

Specifications

Filter Set   Excitation Emission Fluorophores
  UV - Ultraviolet 360-380nm 415nm LP DAPI, ...
  VI - Violet 400-415nm 460nm LP BFP, CFP, ...
  RB - Royal Blue 440-460nm 500nm LP GFP, fluorescein, lucifer yellow, ...
 CY - Cyan490-515nm550nm LPYFP, Venus, ...
  GR - Green 510-540nm 600nm LP DsRed, TdTomato, RFP, ...
Dimensions 5 x 3 x 2
Battery Type 4 C-cell
Bulb Type Two high intensity 3W LED
Burn Time 4 hours at full power
Lamp Life 10,000 hours

Ordering Information

DFP-1 DFP Flashlight with RB and GR LEDs, matching filter glasses, carrying case each 755.00 Add to Cart
DFP-CG DFP Flashlight with CY and GR LEDs, matching filter glasses, carrying case each 815.00 Add to Cart
DFP-RC DFP Flashlight with RB and CY LEDs, matching filter glasses, carrying case each 815.00 Add to Cart
DFP-VC DFP Flashlight with VI and CY LEDs, matching filter glasses, carrying case each 815.00 Add to Cart
DFP-VG DFP Flashlight with VI and GR LEDs, matching filter glasses, carrying case each 815.00 Add to Cart
DFP-VR DFP Flashlight with VI and RB LEDs, matching filter glasses, carrying case each 815.00 Add to Cart
DFP-UC DFP Flashlight with UV and CY LEDs, matching filter glasses, carrying case each 945.00 Add to Cart
DFP-UG DFP Flashlight with UV and GR LEDs, matching filter glasses, carrying case each 945.00 Add to Cart
DFP-UR DFP Flashlight with UV and RB LEDs, matching filter glasses, carrying case each 945.00 Add to Cart
DFP-UV DFP Flashlight with UV and VI LEDs, matching filter glasses, carrying case each 945.00 Add to Cart
NIGHTSEA BlueStararrow11NIGHTSEA™ BlueStar™ Flashlight

The BlueStar flashlight combined with the matched barrier filter glasses is a convenient, powerful tool for exciting fluorescence in many subjects.

The BlueStar uses the latest in high intensity LED technology combined with specialized optics to produce an ultra-tight, ultra-bright 10-degree beam pattern for maximum excitation of fluorescence.

Comes with adjustable, cushioned wrist lanyard, rubber glare guard. Made in USA.

Specifications

Bulb Type High intensity 1W LED Royal Blue, 440-460nm
Burn Time 6 hours at full power
Lamp Life >10,000 hours
Battery 3 C-cell

Ordering Information

BLS-1 BlueStar light plus style FG-RB-1 filter glasses each 205.00 Add to Cart
BLS-2 BlueStar light plus style FG-RB-2 filter glasses each 215.00 Add to Cart
BLS-3 BlueStar light plus style FG-RB-3 filter glasses each 215.00 Add to Cart
NIGHTSEA Barrier Filter Glassesarrow11NIGHTSEA™ Barrier Filter Glasses

Styles 1 and 2 fit over eyeglasses, Style 3 does not.

Glasses meet ANSI Z87.1 impact standards for safety glasses.

 

 

 

Ordering Information

FG-UV Filter glasses, Ultraviolet each 55.00 Add to Cart
FG-VI Filter glasses, Violet each 55.00 Add to Cart
FG-RB-1 Filter glasses, Royal Blue, style 1 each 55.00 Add to Cart
FG-RB-2 Filter glasses, Royal Blue, style 2 each 55.00 Add to Cart
FG-RB-3 Filter glasses, Royal Blue, style 3 each 55.00 Add to Cart
FG-RB-GO Filter glasses, Green Only each 55.00 Add to Cart
FG-CY Filter glasses, Cyan each 55.00 Add to Cart
FG-GR Filter glasses, Green, style 2 each 55.00 Add to Cart

NIGHTSEA™ FAQs arrow13arrow13