Stage Graticules

These stage graticules are intended for the routine calibration of eyepiece patterns, particularly when alternating between objectives on one microscope, or when using the same graticule in different microscopes. Their robust construction makes them ideal for student use and for instructional purposes. The scale or grid is centered on a glass disc mounted in a black anodized aluminum slide 75mm x 24mm x 2mm thick. For applications requiring traceable standards, see B. Stage Calibration Standards.

Two types are available:

• Transmitted light
• Reflected light, as is used with metallurgical microscopes

Table 1: Accuracy and Line Widths of Stage Micrometers

Pattern	Line Width	Accuracy (overall)	Pattern	Line Width	Accuracy (overall)
S1 or PS1	0.005mm	± 0.002mm	S16 or PS16	0.0015mm	± 0.001mm
S2	0.005mm	± 0.00015mm	S20 or 21	0.0025mm	± 0.0015mm
S4 or PS4 S5 or PS5	0.002mm	± 0.0001"	S22	0.0025mm	± 0.0015mm
S8 or PS8	0.002mm	± 0.001mm	S48	0.0027mm	± 0.001mm
S11	0.001mm	± 0.00005"	S78 or PS78	0.003mm	± 0.001mm
S12 or PS12	0.001mm	± 0.001mm	—	—	—

A. Scales and Micrometers

Standard and Crossed Patterns - S1, S2, S4, S5, S8, S11, S12, S16, S20, S21, S22, S48

Pattern	Cat #	Type, Length	Nos. Div.	Div. Size	Qty	Price
S1	68040-01	Horizontal, 10mm	100	0.1mm	each	204.00	Add to Cart
S2	68039-02	Horizontal, 5.0mm	100	0.05mm	each	230.00	Add to Cart
S4	68039-04	Horizontal, 0.1"	100	0.001"	each	230.00	Add to Cart
S5	68039-05	Horizontal, 20mm	200	0.01mm	each	230.00	Add to Cart
S8	68042-08	Horizontal, 1.0mm	100	0.01mm	each	204.00	Add to Cart
S11	68039-11	Horizontal, 0.005"	50	0.0001"	each	346.00	Add to Cart
S12	68044-12	Horizontal, 0.1mm	50	0.002mm	each	346.00	Add to Cart
S16	68039-16	Crossed Scale, 1.0mm	100	0.01mm	each	286.00	Add to Cart
S20	68043-20	Double micrometer	10	0.01mm	each	220.00	Add to Cart
S21	68043-21	Micrometer scale	100	0.5mm	each	230.00	Add to Cart
S22	68039-22	Vertical Scale, 2.0mm	200	0.01mm	each	230.00	Add to Cart
S48	68041-48	No Coverglass, 1.0mm	100	0.01mm	each	226.00	Add to Cart

Certifications

Cat #	Certification	Qty	Price
68047-C	UKAS Certification for SI - S70	each	400.00	Add to Cart
68047-Cert	NPL Certification for SI - S70	each	1,000.00	Add to Cart

S18 Brightfield Stage Micrometer

EMS is proud to introduce the S18 Brightfield Stage Micrometer: Graduated Metric and English Scales for the calibration of measurement instruments across a wide range of magnifications.

This flexible graduated scale stage micrometer is suitable for low magnification stereo inspection microscopes to higher magnification compound microscopes.

Key Features

• Dual Scales: 25mm and 1 Inch
• Graduated Divisions, fine for low magnifications, mid for medium magnifications, and large for high magnifications:
  • 25mm/0.5mm, 5mm/0.1mm, 1mm/0.01mm
  • 1"/0.05", 4/20"/0.01", 1/20"/0.001"
• High Definition, chrome on glass image
• Ideal for you if you use both metric and English measurements

Detail of Scales

The scales are centered on all glass slides 76mm x 25mm x 1.5mm thick.

Specifications

Line Width	Accuracy (overall)	Image Type
0.0025mm (2.5 micron)	.0.002mm	Low reflective chrome surface

Ordering

Pattern	Cat #	Description	Qty	Price
S18	68043-30	S18	each	262.00	Add to Cart
S18	68043-35	S18 with UKAS Certificate	each	895.00	Add to Cart
S18	68043-40	S18 with NPL Certificate	each	1,250.00	Add to Cart

Combined Metric/Imperial Pattern

Pattern	Cat #	Description	Qty	Price
S20	68043-20	Double micrometer scale 2mm in 0.01mm divisions and 0.1 inch in 0.005 inch divisions	each	330.00	Add to Cart

Grouped Graduation Pattern - S21

Pattern	Cat #	Description	Qty	Price
S21	68043-21	Micrometer scale 5mm in 0.5mm divisions, 2mm in 0.1mm divisions, and 0.2mm in 0.01mm divisions	each	242.00	Add to Cart

Counting Grids - S9, S10, S28, S29

Pattern	Cat #	Description	Qty	Price
S9	68045-09	Counting Slide 0.01mm squares.	each	242.00	Add to Cart
S10	68045-10	Counting Slide 0.05mm squares	each	242.00	Add to Cart
S28	68045-28	0.01mm grid / 0.2 x 0.2mm overall	each	260.00	Add to Cart
S29	68045-29	0.01mm grid / 1.5 x 1.5mm overall	each	260.00	Add to Cart

SG7 Particle Analysis Test Calibration Slide

Calibration Test Slide for Particle Sizing with 200 particles of various shapes and sizes.

Pattern	Cat #	Description	Qty	Price
SG7	68045-30	Calibration Test Slide for Particle Sizing	each	225.00	Add to Cart

For Reflected Light

This scale is etched through highly reflective vacuum coated metal. When viewed under vertical illumination, as with a metallurgical microscope, the scale appears black against a bright background.

S1R Reflected Light Stage Micrometer

Reflected light stage micrometer with 10mm scale subdivided into 100 divisions of 0.1mm (100µm).

Pattern	Cat #	Description	Qty	Price
S1R	68047-1R-GR	S1R with Graticule Certificate	each	624.00	Add to Cart
S1R	68047-1R-NA	S1R with UKAS Certificate	each	700.00	Add to Cart
S1R	68047-1R-NP	S1R with NPL Certificate	each	1,200.00	Add to Cart

S4R Reflected Light Stage Micrometer

Reflected light stage micrometer with 0.1" scale subdivided into 0.001" divisions.

Pattern	Cat #	Description	Qty	Price
S4R	68047-4R-GR	S4R with Graticule Certificate	each	675.00	Add to Cart
S4R	68047-4R-NA	S4R with UKAS Certificate	each	720.00	Add to Cart
S4R	68047-4R-NP	S4R with NPL Certificate	each	1,500.00	Add to Cart

Reflected Light – S78

Pattern	Cat #	Description	Qty	Price
S78	68043-78	Micrometer scale 1mm in 0.01mm divisions.	each	237.00	Add to Cart

B. STAGE CALIBRATION STANDARDS

These stage micrometers differ from the standard stage micrometers in that they are available with a certificate giving precise details about their accuracy. These calbrated standards provide traceability for the precise calibration and confirmation of accuracy of optical measuring instruments, which is necessary under ISO provisions.

Micrometer Standards – PS1, PS4, PS4R, PS5, PS8, PS12, PS16, PS78

These standards are different from the stage micrometers listed in the previous section. The glass discs are mounted in stainless steel slides with engraved serial numbers. Each slide is supplied in a polished wooden storage case to distinguish it as a traceable standard of high value. We can arrange for the calibration of its scales and grids to be carried out by the most appropriate laboratory to suit the customers requirements – the choice of laboratory is normally dependent on the nature of the calibration and the accuracy required.

1. Calibration by NPL: The National Physical Laboratory carries out measurements at selected points on the scale and grids and issues a certificate of calibration. Order Code: NP. The NPL is the English equivalent of NIST.
2. Calibration by UKAS accredited laboratory: A UKAS accredited laboratory carries out measurements at selected points on the scales and grids and issues a calibration certificate. Order Code: NA
3. Measurement by Pyser-SGI, Graticules Division: For applications which do not require the accuracy or traceability provided by calibrations carried out by NPL or a UKAS accredited laboratory, we can provide a Certificate of Comparison. The scale or grid is compared with NPL calibrated in-house standards and a statement is provided on the accuracy of the item with respect to these standards. Order Code: GR

Pattern	Cat #	Scale Specs	Description	Qty	Price
PS1	68047-1GR	10mm in 0.1mm	PS1 with Graticules Certificate	each	630.00	Add to Cart
PS1	68047-1NA	10mm in 0.1mm	PS1 with UKAS Certificate	each	725.00	Add to Cart
PS1	68047-1NP	10mm in 0.1mm	PS1 with NPL Certificate	each	1,350.00	Add to Cart
PS4	68047-4GR	0.1" in 0.001"	PS4 with Graticules Certificate	each	625.00	Add to Cart
PS4	68047-4NA	0.1" in 0.001"	PS4 with UKAS Certificate	each	705.00	Add to Cart
PS4	68047-4NP	0.1" in 0.001"	PS4 with NPL Certificate	each	1,400.00	Add to Cart
PS4R	68047-PS4R	0.1" in 0.001"	Micrometer Scale 0.1"; in 0.001 inch divisions for reflected light	each	305.00	Add to Cart
PS4R	68047-PS4R-GR	0.1" in 0.001"	PS4R (reflected) with Grats Certificate	each	620.00	Add to Cart
PS4R	68047-PS4R-NA	0.1" in 0.001"	PS4R (reflected) with UKAS Certificate	each	710.00	Add to Cart
PS4R	68047-PS4R-NP	0.1" in 0.001"	PS4R (reflected) with NPL Certificate	each	1,500.00	Add to Cart
PS5	68047-5GR	20mm in 0.01mm	PS5 with Graticules Certificate	each	725.00	Add to Cart
PS5	68047-5NA	20mm in 0.01mm	PS5 with UKAS Certificate	each	875.00	Add to Cart
PS5	68047-5NP	20mm in 0.01mm	PS5 with NPL Certificate	each	1,650.00	Add to Cart
PS8	68047-8GR	1mm in 0.01mm	PS8 with Graticules Certificate	each	640.00	Add to Cart
PS8	68047-8NA	1mm in 0.01mm	PS8 with UKAS Certificate	each	725.00	Add to Cart
PS8	68047-8NP	1mm in 0.01mm	PS8 with NPL Certificate	each	1,500.00	Add to Cart
PS12	68047-12GR	0.1mm in 0.002mm	PS12 with Graticules Certificate	each	825.00	Add to Cart
PS12	68047-12NA	0.1mm in 0.002mm	PS12 with UKAS Certificate	each	1,400.00	Add to Cart
PS12	68047-12NP	0.1mm in 0.002mm	PS12 with NPL Certificate	each	1,650.00	Add to Cart
PS16	68047-16GR	Crossed, 1mm in 0.01mm	PS16 with Graticules Certificate	each	795.00	Add to Cart
PS16	68047-16NA	Crossed, 1mm in 0.01mm	PS16 with UKAS Certificate	each	1,100.00	Add to Cart
PS16	68047-16NP	Crossed, 1mm in 0.01mm	PS16 with NPL Certificate	each	2,400.00	Add to Cart
PS78	68047-78GR	1mm in 0.01mm	PS78 (reflected) with Graticules Certificate	each	675.00	Add to Cart
PS78	68047-78NA	1mm in 0.01mm	PS78 (reflected) with UKAS Certificate	each	760.00	Add to Cart
PS78	68047-78NP	1mm in 0.01mm	PS78 (reflected) with NPL Certificate	each	1,400.00	Add to Cart

PS52P & PS52N Dual Scale Calibration Slides

Key Features

• New Dual-Scale Calibration Slides
• 2" Imperial (English) and 50mm Metric Scales on a Single Slide
• Positive and Negative Versions
• Unique Serial Number for Traceability
• Available with Internationally Traceable Certificates of Calibration

EMS has introduced two new calibration slides that have the benefit of dual imperial/metric scales. The PS52P is for transmitted light applications and has a bright chrome positive image. The PS52N has a negative pattern, formed in low reflective chrome for incident light applications, to give excellent contrast. Both are ideal for calibrating optical products with a large field of view, such as stereo microscopes or imaging systems.

Pattern	Cat #	Description	Qty	Price
PS52P	68040-05	Dual Micrometer Calibration Slide, 76mm x 25mm, positive image, 50mm in 0.1mm divisions and 2" in 0.005" divisions, serial numbered, supplied in wooden case	each	530.00	Add to Cart
	68040-06	PS52P with UKAS Certificate	each	1,200.00	Add to Cart
PS52N	68040-07	Dual Micrometer Calibration Slide, 76mm x 25mm, negative image, 50mm in 0.1mm divisions and 2" in 0.005" divisions, serial numbered, supplied in wooden case	each	550.00	Add to Cart
	68040-08	PS52N with UKAS Certificate	each	1,115.00	Add to Cart

H.S.E./N.P.L. Test Slide for Phase Contrast Microscopy – S84

This test slide is made in the UK and is under license from the National Physical Laboratory. It is an epoxy replica of a master slide produced and certified by that laboratory. The replicas are mounted on microscope slides of 1.2mm thickness with cover glass of 0.17mm thickness. This information is transferred to the accompanying table, which indicates the maximum phase change passing through test objects to test slide. A satisfactory system will detect block 5. Full details are supplied with the slide.

Pattern	Cat #	Description	Qty	Price
S84	68038-84H	HSE Test Slide for Calibration in Asbestos Analysis	each	1,625.00	Add to Cart

Universal Calibration Slide – PS20

Calibration of microscopes and image analysis systems is becoming more sophisticated, with the requirement being for a variety of image patterns to satisfy the numerous parameters. We're now introducing a new multi-function calibration standard specifically for these applications.

Multiple images on a slide provide the most cost-effective solution to calibration and resolution checking of microscopes and image analysis systems. The combination of scales, dots, circles, squares, rulings, grids and angles can be supplied with an internationally traceable certificate of calibration for those who require ISO conformity.

Each glass slide has a permanent serial number and can be supplied with full or partial UKAS Certificate of Accuracy.

Starting from a fixed 'Datum Point' mark, each individual pattern or array can be located using X, Y, coordinates.

General Tolerance (microns)

Feature size	≤ 10	10 - 50	50 - 127	127 - 250	>250
Tolerance	0.5	1.0	1.3	1.9	2.54

Specifications

Coating	Enduring evaporated chrome image
Optical density	>2.54
Substrate	Soda lime glass
Size	76mm x 25mm x 1.5mm
Package	Polished wooden case

PS20 Universal Calibration Slide Image Details

ID	Pattern Name	Location	Description
A*	Concentric Circles	X =2; Y = 10	1, 2, 3, 4, 5mm Circles with Cross Line & circle identifier. Line width 20µm
B*	Concentric Squares	X=10; Y=10	1, 2, 3, 4, 5mm Squares with Cross Line & circle identifier. Line width 20 nm
C*	Line Grating	X=18; Y=10	12.5 Line Pairs permm (40µm line 40µm space)
D*	Line Grating	X=26; Y=10	50 Line Pairs permm (10µm line 10µm space)
E*	Half Protractor	X=34; Y=10	15 Spacing, Line width 20µm
F*	Grid Array Coarse	X=40; Y=10	5mm square array with 0.5mm divisions and central 2mm square with 0.25mm divisions. Line width 20µm
G*	Grid Array Fine	X=48; Y=10	5mm square array with 0.1mm divisions and central 2mm square with 0.05mm divisions. Line width 8µm divisions and central 2mm square with 0.05mm divisions. Line width 8µm
H*	Dot Array	X=56; Y=10	Dot diameter 0.25mm, dot centre to centre spacing 0.050mm – 11x11 grid = 121 dots to centre spacing 0.050mm – 11x11 grid = 121 dots
I*	Geometric progression of Opaque Dots	X=2; Y=4	Line array of dots or square shapes, of either clear or opaque. Reducing in size in a Root 2 progression for the purposes of edge threshold detection to enable an image analyzer to measure the size correctly, or general shape size comparison.
J*	Geometric	X=17; Y=4	Root 2 progression of 21 dots or square shapes, from 3.5µm to 3.5µm.
K*	Geometric progression of Clear Dots	X=32; Y=4	Nominal size in mm Dot/square size – Large to small inmm 3.5833; 2.5338; 1.7917; 1.2669; 0.8959; 0.6335; 0.4479; 0.3167; 0.2240; 0.1584; 0.1120; 0.0792; 0.0560; 0.0396; 0.0280; 0.0198; 0.0140; 0.0099; 0.0070; 0.0049; 0.0035
L*	Geometric progression of Clear Squares	X=47; Y=4
M*	Vertical Scale Fine Variable	X=63	Overall Scale length 10mm. 5mm in 0.5mm divisions. Line width 20µm 4mm in 0.1mm divisions. Line width 10µm 1mm in 0.01mm divisions. Line width 3 µm
N*	Horizontal	X=0; Y=0	Scale length 62mm in 2mm Scale Coarse divisions, subdivided in 1mm divisions with a 20µm line width.

*If you require a calibration certificate for any or all of the points A-N, please state which points and we will send you a quote.

Pattern	Cat #	Description	Qty	Price
PS20	68053-20	Universal Calibration Slide PS20	each	410.00	Add to Cart

PS25 Hardness Tester Calibration Slides – Vickers/Rockwell

Reflected light calibration slide with diamond shapes and scales for calibrating Vickers and Rockwell hardness testers.

For Vickers and Rockwell methods, we offer the PS25 which has a series of diamond shapes of varying size and x-y scales. Each of the markings on the slide is clearly identified with its size. The PS25 has a glass disc with the image precision marked in vacuum deposited chrome and this is cemented into a stainless steel slide mount, making the item very durable. The slide has a unique serial number indelibly marked on the slide mount and can be supplied with an internationally traceable certificate of calibration.

PS25 Pattern Detail

• Diamonds: (Point to point,mms) 0.5, 0.3, 0.2, 0.1. 0.05. 0.02
• Scales: Horizontal & vertical, 2mm in 0.1mm divisions

This product is supplied in a polished wooden case.

Pattern	Cat #	Description	Qty	Price
PS25	68049-05	Calibration Slide for Hardness Testers -Vickers/Rockwell	each	360.00	Add to Cart
PS25	68049-06	PS25 with UKAS Certificate	each	1,250.00	Add to Cart

PS26 Hardness Tester Calibration Slides – Brinell

Reflected light calibration slide with circle shapes and scales for calibrating Brinell hardness testers

For Brinell methods, we offer the PS26 which has a series of circles to represent the ball indentation shape of varying size and x-y scales. The PS26 has a precision marked chrome deposition image on a glass slide. Each of the markings on the slide is clearly identified with its size. The slide can be supplied with an internationally traceable certificate of calibration. This product is supplied in a polished wooden case.

PS26 Pattern Detail

• Circles: (Diameter, mms) 5.0, 2.5, 1.0, 0.7, 0.3
• Scales: Horizontal & vertical, 2mm in 0.1mm divisions

Pattern	Cat #	Description	Qty	Price
PS26	68049-15	Calibration Slide for Hardness Testers - Brinell	each	350.00	Add to Cart
PS26	68049-18	PS26 with UKAS Certificate	each	1,250.00	Add to Cart

High Definition Long Linear Combination Glass Scales

Superior PS-XO Versions

• High definition images for accurate calibration - line edge or line center
• Bright chrome image on green float glass
• Line width 0.03mm
• 300mm, 200mm, 150mm and 100mm lengths, all in 0.1mm divisions
• Extended 0.5mm, 1.0mm and 5.0mm lines allow calibrations in those increments, too

Pattern	Cat #	Description	Qty	Price
PS300XO	68049-20	300mm combination scale, 330mm x 30mm x 6mm, 300mm ruling in 0.1mm divisions, 1mm dots at 10mm centers, crosses at 10mm centers	each	1,000.00	Add to Cart
	68049-21	As above but with UKAS certificate of calibration, 10 points measured on scale	each	1,500.00	Add to Cart
PS200XO	68049-22	2 00mm combination scale, 230mm x 30mm x 6mm, 200mm ruling in 0.1mm divisions, 1mm dots at 10mm centers, crosses at 10mm centers	each	1,000.00	Add to Cart
	68049-23	As above but with UKAS certificate of calibration, 10 points measured on scale	each	1,500.00	Add to Cart
PS150XO	68049-24	150mm combination scale, 180mm x 30mm x 6mm, 150mm ruling in 0.1mm divisions, 1mm dots at 10mm centers, crosses at 10mm centers	each	925.00	Add to Cart
	68049-25	As above but with UKAS certificate of calibration, 10 points measured on scale	each	1,200.00	Add to Cart
PS100XO	68049-26	100mm combination scale, 130mm x 30mm x 6mm, 100mm ruling in 0.1mm divisions, 1mm dots at 10mm centers, crosses at 10mm centers	each	825.00	Add to Cart
	68049-27	As above but with UKAS certificate of calibration, 10 points measured on scale	each	1,200.00	Add to Cart

High Definition Long Linear Glass Scales

• Parallax free readings – ideal for measuring systems and CMMs
• High levels of accuracy and pattern definition. Hard wearing scales in vacuum deposited chrome on
  substantial glass substrates. For measurement and calibration of instruments and standards.
• Line width 0.07mm
• Overall accuracy within 0.007mm

All available with Certificate of Calibration.

Pattern	Cat #	Description	Qty	Price
PS100HS MKII	68050-01	100mm long scale in 1mm divisions, 130mm x 30mm x 6mm	each	595.00	Add to Cart
PS150HS MKII	68050-02	150mm long scale in 1mm divisions, 180mm x 30mm x 6mm	each	695.00	Add to Cart
PS200HS MKII	68050-03	200mm long scale in 1mm divisions, 230mm x 30mm x 6mm	each	800.00	Add to Cart
PS300HS MKII	68050-04	300mm long scale in 1mm divisions, 330mm x 30mm x 6mm	each	825.00	Add to Cart

Standard Definition Long Linear Glass Scales

• Parallax free readings - ideal for profile projectors
• High levels of accuracy and pattern definition. Hard wearing scales in vacuum deposited chrome on substantial glass substrates. For measurement and calibration of instruments and standards

Pattern	Cat #	Description	Line Width	Accuracy Within	Qty	Price
PS50	68051-10	50mm micrometer scale in 0.1mm divisions, 75mm x 75mm x 3mm	0.012mm	0.007mm	each	495.00	Add to Cart
PS100	68051-11	100mm long scale in 0.1mm divisions, 130mm x 30mm x 6mm	0.03mm	0.015mm	each	495.00	Add to Cart
PS150	68051-12	150mm long scale in 0.1mm divisions, 180mm x 30mm x 6mmn	0.03mm	0.015mm	each	600.00	Add to Cart
PS300	68051-13	300mm long scale in 0.1mm divisions, 330mm x 30mm x 6mm	0.03mm	0.025mm	each	625.00	Add to Cart
PS500	68051-14	500mm long scale in 0.1mm divisions, 530mm x 30mm x 6mm	0.07mm	0.025mm	each	1,500.00	Add to Cart
PS1000	68051-15	1,000mm long scale in 0.1mm divisions, 1,060mm x 30mm x 6mm	0.07mm	0.025mm	each	2,600.00	Add to Cart

Measuring Scales

These are standard glass scales for in-contact measurements. Ideal for direct vision, for pocket magnifiers and for use in measuring profiles on projector screens.

Pattern	Cat #	Description	Line Width	Qty	Price
P6	68051-21	Contact nonparallax scale, 100mm in 0.1mm divisions, 125mm x 25mm x 3.0mm	0.025mm	each	500.00	Add to Cart
P16	68051-22	Contact nonparallax scale, 300mm in 0.5mm divisions, 330mm x 30mm x 6.0mm	0.10mm	each	625.00	Add to Cart

Calibration Grids

For checking two-dimensional instruments for straightness and accuracy. The patterns are produced in vacuum deposited chrome on glass. Lines are every 10mm with a central 20mm subdivided in 1mm rulings.

Specifications

	PGR 100
Overall Divided Area	100 x 100mm
Glass Size	120 x 120mm
Glass Thickness	6mm
Line Width	0.008mm
Linear Straightness	0.002mm
Angular Accuracy	within 5 seconds

Ordering

Cat #	Description	Qty	Price
68051-31	PGR100 Calibration Grid	each	1,250.00	Add to Cart

The Grid Dot Array

Green Float glass, high reflective Chrome image

The ideal product for testing image area, distortion, field flatness, and other parameters in optical and imaging systems. The three array areas give options for different magnifications or field size.

The R76, a unique product with three dot arrays to suit different magnification or image areas.

Three image areas – dot size and pitch different in each area

• 12 x 9 array of 1mm dots at 5mm pitch
• 16 x 12 array of 0.5mm dots at 2mm pitch
• 24 x 18 array of 0.2mm dots at 1mm pitch

Cat #	Description	Size	Qty	Price
68073-R76	Grid Dot Array, 3 image areas	101 x 101 x 2.2mm	each	460.00	Add to Cart

Image Analysis Standard (Reference Stage Graticule)

This high precision image analysis standard provides four test areas designed for calibrating image analysis systems and identifying deviations and distortions in optical imaging systems.

The standard, which can also be used as a high precision stage micrometer, is supplied with recommendations for its use and an individual certificate of calibration.

It is produced on a 75mm x 25mm slide and has a square grid accuracy of ±0.1µm and a dot accuracy of ±0.3µm (except for the smallest and largest two dots on the root-2 array, where accuracy is ±0.5µm).

The Four Test Areas
	400µm x 400µm square grid which is subdivided into 200, 100, 50 and 25 micron squares provides a means of detecting gross image distortions, and can be used as an accurate two dimensional stage micrometer.

A 20 x 17 array of nominally 15µm diameter dots can be used to identify lens distortions. i.e. to set the field of view to eliminate edge distortion.

A log-normal distribution array of 100 spots ranging from 4.5µm to 27µm diameter enables the mean and standard deviation to be determined and compared with the certified values. This is an idealized distribution of maximum dynamic range for a full screen.

A root-2 array of spots from 3µm to 48µm diameter is used for determining the threshold levels of cameras and microscope systems.

Ordering

Cat #	Description	Qty	Price
68049	Reference Stage Graticule 75mm x 25mm slide.	each	3,950.00	Add to Cart

C. Vibration

FOE PPL Dot – S25

The pair of dots, which appear to merge into a single dot, determines the amount of vibration of the slide in the appropriate axis. The pattern on the S25 is an array of 20 pairs of dots converging on a single dot. The distance between each dot pair increases by 0.001 inch to a maximum of 0.02 inch, pairs being equispaced 0.25 inch.

Pattern	Cat #	Description	Qty	Price
S25	68038-25	FOE PPL Dot Vibration Test	each	29.00	Add to Cart

D. Volume/Counting Cells

These are used to create precise volumes of liquid to enable counting of particles in a specified volume of liquid. A thick slide (glass or plastic) has an area cut into its surface that will hold precise volumes of bathing fluid. The shallow well facilitates easy counting of the contained particles. In use, a single drop of liquid is placed on the slide and a coverglass is placed on the platform, providing equal and accurate subdivisions of the fluid, each of which can be observed for particle counting.

Sedgewick Rafter and Cover Glass – S50, S51, S52

For use in water analysis, culture inspection, and any fluid where particles per unit volume need to be determined.

Available in two versions

1. S50 is an economically priced plastic cell.
2. S52 is made from glass, with a chromium surface image. This is intended for professional use and when using phase contrast. In each type, a central cell is filled with fluid and a coverglass limits the volume to 1 milliliter. The grid subdivides this into micro-liters.

S51 is the spare cover glass for S50 and S52. Each cell is supplied with one cover glass.

Pattern	Cat #	Description	Qty	Price
S50	68050-50	S50, Sedgewick Rafter, Plastic	each	53.00	Add to Cart
S52	68050-52	S52, Sedgewick Rafter, Glass	each	260.00	Add to Cart
S51	68050-51	S51, Cover Glass for S50 & S52	each	16.00	Add to Cart

Howard

The Howard cell is a glass slide 76mm x 35mm with a central circular island and is used for counting mold fibers and spores in fruit juices, especially from tomatoes. With the K20 coverglass in place, a 0.1mm thickness of liquid is contained over the central island. The coverglass has 25 calibrated fields of 1.382mm diameter through which to view the particles. This coverglass removes the necessity of precise adjustment of the microscope and eyepiece graticule in the original Howard method.

Pattern	Cat #	Description	Qty	Price
S60	68051-60	Howard Cell for Fruit Juices	each	345.00	Add to Cart
K20	68051-20	K20 Calibrated Cover Glass	each	102.00	Add to Cart

E. Finder Graticles

Finder graticules are used to swiftly and accurately give a position of reference to an area of interest on a specimen.

The England Finder – S7

S7 is a glass slide 75mm x 26mm (3 x 1") marked over the top surface in a way that a referenced position can be directly read relative to the locating edges. That means it is marked with a square grid at 1mm intervals. Each square contains a center ring bearing reference letters and numbers. The remainder of the square is subdivided into four segments numbered 1 to 4.

Reference numbers run horizontally 1 to 75, and letters vertically A to Z (omitting I). The main locating edge is the bottom of the slide, which is used in conjunction with either the left or right vertical edge of the slide.

All England Finders produced for over 40 years are identical. The purpose of the finder is to give the microscopist an easy method of recording the position of a particular field of interest, so that another person in another laboratory can relocate at a later date, or the same position, or when using any other England Finder on any other microscope.

The location of the arrows is identical for all England Finder slides. Mark a label on the left hand side of the specimen slide indicating the orientation to be repeated. By replacing the specimen slide with England Finder, taking care not to disturb the position, feature of interest can be noted. The feature can also be relocated at another place or time by reversing the procedure. A total of 100 positions on a slide can be accurately located.

Pattern	Cat #	Description	Qty	Price
S7	68048-07	The England Finder	each	262.00	Add to Cart

The Halton Finder – S30

The Halton Finder features the same pattern as the England Finder but only covers a 5mm x 5mm area in the form of a stage graticule.

Pattern	Cat #	Description	Qty	Price
S30	68049-07	The Halton Finder	each	288.00	Add to Cart

Makler

For Rapid Semen Analysis

The Makler counting chamber is only 10 microns deep, which is one tenth the depth of other hemacytometers, making it the shallowest of known chambers. This quality chamber is constructed from two pieces of optically flat glass: the first is the chamber, the second is the cover glass which has a fine grid pattern of 1mm squares and a center area further subdivided into 0.1mm squares. Four quartz pins of precise height hold the cover glass to give an exact trapped specimen depth of 10 microns.

Cat #	Description	Qty	Price
68052-01	Makler Counting Chamber	each	1,800.00	Add to Cart

Optical Resolution Charts, Ronchi Rulings and Grids