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Electron Microscopy Sciences

Technical Data Sheets

PHEM Buffer

EMS #11162, 11163

PHEM buffer has been used primarily for tissues and cell cultures being processed for immunocytochemical studies. Most antigens, especially intra cellular ones, stain better using PHEM than those processed using PBS, it also has a more limited effect on biochemical reactions and enzymes. Very good preservation of cellular ultrastructure, notably microtubules, is also an advantage of PHEM use.

Desired Fixative 2.5% Glutaraldehyde, 1.5% Paraformaldehyde in 0.1M PHEM buffer

Glutaraldehyde 10 ml 25% 100 ml, 2.5% 25% x V1 = 2.5% x 100 ml - V1 = 10 ml
Paraformaldehyde 32% 100 ml, 1.5% 32% x V1 = 1.5% x 100ml - V1 = 4.7 ml
0.2 M PHEM stock 100 ml, 0.1M 0.2 M x V1 = 0.1 M x 100 ml-V1 = 50 ml
QS with distilled water to 100 ml. Approximately 35.3 ml

Product Information

PHEM Buffer