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Electron Microscopy Sciences

Technical Data Sheets

Bismuth Stain Kit for Specific En Bloc Staining

EMS #11430

Bismuth Stain Kits for Specific En Bloc Staining Kit consists of:

  • 10 x 5 ml Solution A (Sodium Tartrate, NaOH and Bismuth Subnitrate)
  • 10 x 10 ml Solution B (.2M Triethanolamine-HCl buffer, pH 7.0)

Procedures

Staining solution:

Mix Solution A and B together (One part of A into two parts of B) then adjust to pH 7.0 with 1N HCl.

Aldehyde-fixed tissue blocks are rinsed in the above buffer and stained en-bloc with Bismuth for one hour at room temperature on a gentle rotary shaker. Postfixation is carried out with 1% Osmium Tetroxide in 0.05 M Sodium Cacodylate buffer, pH 7.2.

Post-staining is not needed.

Reference

Locke & Huie, (1977). Bismuth staining for Light and Electron Microscopy. Tissue Cell 9, 347.

Additional Technical Data Sheets

Bismuth Stain Kit for Enhancing Contrast in Uranyl Acetate Treated Tissue
Bismuth Stain Kit for Mucosubstances and Polysaccharides

Product Information

Bismuth Stain Kits for Specific En Bloc Staining