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Electron Microscopy Sciences

Technical Data Sheets

Giemsa Stain For Glycol Methacrylate Sections

EMS Catalog # 14653-14654

Technovit H7100 / H8100 Giemsa Stain

This Giemsa stain works well for a variety of tissues.

Procedure

  1. Stain in Giemsa working solution at room temperature for 1½ hours.
  2. Rinse in dilute acetic acid (four drops in 100 ml DI water) for two seconds.
  3. Dip in 96% alcohol.
  4. Dip in 96% alcohol.
  5. Rinse in isopropyl alcohol three times for two minutes each.
  6. Clear in xylene and mount.

Results

Nuclei violet
Cytoplasm blue
Erythrocyten pink

Solutions

Giemsa Solution:

  • 0.15 g Azure A
  • 0.30 g Methylene Blue
  • 0.36 g Eosin Y
  • 0.04 g Phloxine B
  • 50 ml Glycerine
  • 40 ml Methanol
  • Add dyes to Glycerine and methanol, mix well. Incubate overnight in 55-58°C oven stirring occasionally. Stable for year.

Giemsa Working Solution:

  • 4 ml Giemsa stock solution
  • 40 ml phosphate buffer solution

Phosphate Buffer Solution:

  • 7.25 g Sodium phosphate, monobasic
  • 2.75 g Sodium phosphate, dibasic
  • 1000 ml Distilled water
  • 10 ml 10% Triton X-100
  • Store at 4°C.

Product Information

Technovit Glycol Methacylate Kits H7100 / H8100