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Electron Microscopy Sciences

Technical Data Sheets

Prussian Blue Stain for Glycol Methacrylate Sections

EMS Catalog #14653 - 14654

Technovit H7100 / H8100 Prussian Blue Stain

Procedure

  1. Stain in Potassium Ferricyanide solution at 60°C for 15 minutes. Filter solution after heating and before use.
  2. Wash in distilled water.
  3. Stain in Safranin O for two to five minutes.
  4. Wash in 1% acetic acid.
  5. Dehydrate in 96% alcohol two times then 100% alcohol.
  6. Clear in xylene and mount.

Results

Nuclei
red
Hemosiderin
blue / green

Solutions

Potassium Ferricyanide Solution:

  • Potassium Ferricyanide - 1 g
  • Distilled water - 50 ml
  • 2% Hydrochloric acid - 50 ml

Safranin O:

  • Safranin O (C.I. #50240) - 0.2 g
  • 1% acetic Acid - 100 ml

Citation

Gerrits, P.O. and Smid, L., "Staining Procedures for Tissues Embedded in 2-Hydroxyethyl Methacrylate", Heraeus Kulzer

Product Information

Technovit Glycol Methacylate Kits H7100 / H8100