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Electron Microscopy Sciences

Technical Data Sheets

Van Gieson's Solution

EMS Catalog #26046

Van Gieson's Method for Collagen Fibers 

Fixation:

Any

Sections:

Paraffin @ 6 microns or Celloidin

Staining:

  1. Deparaffinize and hydrate to distilled water
  2. Weigert's Working Hematoxylin* for 10 minutes.
  3. Wash in distilled water.
  4. Stain 1-3 minutes in Van Gieson's Solution (#26046-05)
  5. Dehydrate in 95% alcohol, absolute alcohol, two changes each. Clear in two changes xylene.
  6. Mount with Permount (#17986-01) or equivalents.

*Weigert's Working Solution is prepared by mixing equal parts of Weigert's Iron Hematoxylin A (EMS Cat #26044-05) and Weigert's Iron Hematoxylin B (EMS Cat. #26044-15)

Staining Results:

Collagen: Red

Smooth and striated muscle: Yellowish to Brownish

Cornified epthelium: Yellow

Hyalin: Yellow

Nuclei: Blue to Black

References:

Clark, G., (ed.) Staining Procedures 3 rd Ed., Williams & Wilkins Co. Baltimore, c. 1973, p.50

Luna, L., (ed.) AFIP Manual of Histological Staining Methods, 3 rd Ed., New York . McGraw Hill Publication, c. 1968, p. 76.

Van Gieson, I. , New York Med. J., 50:57-60, 1889.

Mallory, F.B., Pathological Technique, W.B. Saunders Co., Philadelphia 1938.

Verhoeff's Elastic Tissue Stain 

Fixation:

10% Formalin or Zenker's Stock Solution (EMS Cat. #64123)

Section:

Paraffin @ 6 microns.

Staining Procedure:

  1. Deparaffinize and hydrate to distilled water.
  2. Place in staining solution for 15 minutes. Staining solution should be prepared fresh.
    Combine: 25 ml Iron solution.
    10 ml Potassium Iodide, 3%, aqueous.
    3.5 ml Alcoholic Hematoxylin, 10%.
    Use once and discard.
  3. Wash in tap water. Differentiate in Iron Solution, diluted 1-4 with distilled water
  4. Counter stain with Van Gieson's Solution, 3 - 5 minutes.
  5. Dehydrate, clear and mount.

Results:

Elastic Fibers: Intensely blue-black

Nuclei: Blue to black

Collagen: Red

Smooth and Striated Muscle: Brownish

References:

Clark, G., Ed: Staining Procedures. 3 rd ed., Baltimore : William and Wilkens Co. c. 1972, p. 61

AFIP Manual of Histologic Staining Techniques. 3 rd ed., Ed. G.. Luna: New York : McGraw-Hill Publications, C. 1968, p. 76

Ordering Information

Van Gieson's Solution
Zenker's Stock Solution
Weigert's Iron Hematoxylin