View Cart || Request a 2021 Catalog

Electron Microscopy Sciences

Technical Data Sheets

Giemsa For H-Pylori And Mast Cells (microwave )

EMS Catalog #26110


10% Buffered Neutral Formalin


3 microns


  1. Deparaffinize and hydrate to distilled water.
  2. Place promptly in Methanol for 2 minutes.
  3. Preheat Working Geimsa solution in microwave on high for 45 seconds. Filter the hot solution into a coplin jar containing the slides. Stain for 2 minutes.
    To prepare working solution mix:

    •  7.0 ml Geimsa Stock
    •  38 ml Distilled water
    •  5 ml Methanol
    Prepare fresh- do not reuse!!
  4. Rinse quickly in distilled water
  5. Dip one slide at a time in Acetic Acid, 1% .
  6. Rinse quickly in distilled water.
  7. Rinse in 3 changes of Methanol.
  8. Clear in Xylene and mount.

Stain Results

Red Blood Cells Pink
Nuclei Blue
H-Pylori, fungi and other bacteria Blue
Mast Cells Purple
Rickettsia Purple

NOTE: The use of methanol to dehydrate stained sections prior to mounting seems to provide lighter staining of surrounding tissue, offering better contrast with stained organisms.


Helicobacter pylori in peptic ulcer disease, NIH Consensus Statement Online, January 1994, 12 (1): 1:23 . Luna l AFIP 3 rd ed. McGraw Hill, 236.

Fail, Rena , AS , HT (ASCP) Medical University of South Carolina , Histologic . Vol. XXXIV, No. 2, Nov. 2001. P 32.

Product Information

Giemsa Stock Solution
Acetic Acid, 1%
Giemsa For H-Pylori And Mast Cells