May Grunwald Giemsa For H-Pylori
EMS Catalog #26114
Fixation
10% Buffered Neutral Formalin
Sections
3 microns
Procedure
- Deparaffinize and hydrate to distilled water.
- Place promptly in methanol (methyl alcohol), two changes, three times each.
- Stain in Jenner's Working solution for 6 minutes.
To prepare working solution mix:
• 1 part Jenners Stain
• 1 part Distilled water
Prepare fresh!
- Stain in Working Geimsa solution for 10 minutes.
To prepare working solution mix:
• 2.4 ml Geimsa Stock
• 50 ml Distilled water
Prepare fresh-do not reuse!
- Differentiate in Acetic Acid, 0.1% (A-114-3) one slide at a time, gently agitate, checking often under microscope until defined.
DO NOT OVER DIFFERENTIATE! - Dehydrate quickly in tert-Butyl Alcohol to Xylene
- Mount.
Stain Results
| H-pylori | Blue |
| Background | Light purple |
References
Carson, F. Histotechnology: A Self-Instructional Text . 1980-109.
Product Information
Jenner’s Stain Stock Solution
Giemsa
Stock Solution
Acetic
Acid, 0.1% Solution
May
Grunwald Giemsa For H-Pylori
