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Electron Microscopy Sciences

Technical Data Sheets

Giemsa Thin Film Stain

EMS Catalog #: 26153-Series

Fixation:

Streak a drop of blood, leukocytes, rickettsiae culture, pus or other exudates over a sterile glass slide by means of another slide or cover glass. Fix film in Methyl Alcohol for 2-3 minutes.

Staining:

  1. Place in Ethyl or Methyl Alcohol for 5 – 7 minutes. Air dry.
  2. Stain in the Giemsa Thin Film Staining solution (#26153-01)*. Because different cells accept the stain at varying rates, prepare 3 – 4 sample slides and stain from 15 – 20 minutes. Some membranous structures (Trypanosomes) may require 1 – 2 hour periods.
    1. To prepare Giemsa Thin Film Staining solution from Giemsa Stock solution (#26153-03), mix before use:
      Giemsa Stock Solution (#26153-03) - 1 ml
      Phosphate Buffer (#26153-02) -50 ml
  3. Wash in distilled water and air dry.

Stain Results:

Nuclei, additional Leukocyte features are similar to Wright Stain results. Reddish Purple
Plasmodic cytoplasm Blue
Chromatin Red

References:

Clark, G., Staining Procedures, Williams and Wilkins Co. Baltimore, 3rd, c. 1973, p. 125

Product Information

Giemsa Thin Film Stain