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Electron Microscopy Sciences

Technical Data Sheets

Pizzolato's Method For Calcium Oxalate

EMS Catalog #26212


Buffered Neutral Formalin, 10%


Paraffin @ 6 microns


  1. Deparaffinize and hydrate to distilled water.
  2. Pour enough working silver nitrate-hydrogen peroxide solution* onto slides to cover sections. Place a 60-watt light bulb 6 inches from sections for 30 minutes. Rinse thoroughly with distilled water.

    Prepare working solution by mixing, just before use, equal quantities of
    •  Silver Nitrate, 5%
    •  Hydrogen Peroxide, 30%.
  3. Counterstain for 5 minutes in Nuclear Fast Red Solution. Rinse in distilled water.
  4. Dehydrate in 95% alcohol, absolute alcohol and clear in Xylene, two changes each.
  5. Mount with Permount.

Stain Results

Calcium oxalate Black
All other tissue elements Red


Note: If a large amount of egg albumin section adhesive is present, numerous gas bubbles will develop during the light bulb staining treatment (step 2). This tends to inhibit the reaction. Should this occur, pour off the mixture and add fresh solution.


Pizzolato, P., J. Histochem. Cytochem., 12:333-336, 1964.

Luna, L.G., (ed), Manual of Staining Methods of the AFIP, 3 rd edition, McGraw-Hill, NY, c 1968, p 177.

Online Ordering

Silver Nitrate, 5% Aqueous, Hydrogen Peroxide, 30%, and Nuclear Fast Red (Kennechtrot) Solution for Pizzolato's Method For Calcium Oxalate are available online from the EMS Catalog. For ordering or product information, click here.