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Electron Microscopy Sciences

Technical Data Sheets

Maximow's Method for Bone Marrow

EMS Catalog #: 26252-Series

Fixation:

Zenker’s / Formalin

Sections:

Paraffin at 6 microns

Staining Procedures:              

  1. Deparaffinize and hydrate to distilled water.
  2. Mayer’s Hematoxylin (#26252-01) for 15 minutes. Wash in tepid tap water for 15 minutes.
  3. Stain in working solution overnight. Prepare working solution fresh each time as follows:
    1. Eosin Y, 0.1%, Aqueous (#26252-02) - 10 ml
      Triple distilled water or Phosphate Buffer (#26252-04) - 100 ml
      Azur II, 0.1%, Aqueous (#26252-03) - 10 ml
      Glacial Acetic Acid - 2 drops
  4. Differentiate in 95% alcohol until blue ceases to come out into alcohol and erythrocytes and collagen are pink.
  5. Dehydrate in absolute alcohol, clear in xylene, two changes each.
  6. Mount with Permount (#17986-01).

Stain Results:

Nuclei Blue
Basophile leucocyte, mast cell Purple to Violet
Cartilage Purple
Erythrocytes, eosinophile granules Pink
Cytoplasm Blue to Pink

References:

AFIP, Manual of Histologic Staining Methods, 3rd edition, Ed. L. Luna: New York: McGraw Hill Publications, c. 1968, p. 120

Mallory, F.B., Pathological Technique, New York, Hafner Publishing Co., 1961, p. 196.

Product Information

Maximow's Method for Bone Marrow