Sweat-Puchter Method For Amyloid
EMS Catalog #26303
Fixation
10% Buffered Neutral Formalin
Sections
Paraffin at 6 microns. 12 micron sections may be used to demonstrate minute amyloid deposits. Staining is brighter on 12 micron sections.
Staining
- Deparaffinize and hydrate to distilled water.
- Wash in running water 5 minutes. Mordant in Buffered Neutral Formalin overnight. Wash in water for 15 minutes.
- Alkaline Alcohol Solution for 1 hour. Rinse in distilled water for 10 seconds.
- Stain in preheated Sirius Red Solution for 90 minutes @ 60 °C. Rinse briefly in two changes of Borate Buffer. Wash in running water for 5 minutes.
- Stain in Hematoxylin Solution for five minutes. Wash in running water for at least 20 minutes to prevent fading of stain.
- Hydrate rapidly in Absolute Alcohol. Clear in three changes of Xylene. Mount.
Results
| Amyloid | Pink to Red |
| Nuclei | Blue |
| Elasticia | Pink to Red |
| Background | Unstained |
References
Sweat, F. and Puchtler, H.: Arch. Path. 80:613, 1965.
AFIP Manual of Histologic Staining Methods, 3 rd edition, Ed L. Luna: New York : McGraw Hill Publications, c. 1968, p. 154.
Product Information
Sirius Red
F3BA Solution
Mayer
Hematoxylin
Borate
Buffer
Alkaline
Alcohol
Sweat-Puchter
Method
