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Electron Microscopy Sciences

Technical Data Sheets

Lacquers's Method for Alcoholic Hyalin

EMS Catalog #: 26306-Series


10% Buffered Neutral Formalin or Zenker’s


Cut Paraffin, 6 microns.

Staining Procedures:

  1. Deparaffinize and hydrate to distilled water.
  2. Stain in Mayer Hematoxylin (#26306-01) for 15 minutes. Wash in running water for 15 minutes.
  3. Flood slides with Acid Fuchsin -:Aniline  Solution (#26306-02). Shake bottle well before using, solution will separate upon standing, and heat gently until aniline fumes escapes, view against a dark background. Let stand for 15 minutes. Wash in water, 3 dips.
  4. Differentiate in Alcoholic Picric Acid (#26306-05) until only hyaline and red cells remain red, and collagen is faint gray or unstained. Wash in running water for 3 minutes.
  5. Mordant in 1% Phosphomolybdic Acid (#26306-04) for 4 to 18 hours.
  6. Counterstain in Light Green Solution (#26306-03) for 1 hour. Wash in running water for 1 minute.
  7. Fibers are seen as distinct fibrils.
  8. Dehydrate in 95% alcohol, absolute alcohol, and clear in xylene, two changes each.
  9. Mount with Permount (#17986-01).

Stain Results:

Mallory Bodies Bright Red
Erythrocytes Red
Cystoplasm Pale Brown
Bile Pigment Green
Protinaceous material seen occasionally in some liver cells Red
Hemosiderin and lipofichsin Unstained


Lacquer, G.I., Amer. J. Clin. Path., 20:689-690, 1950.

Luna, L.G. (ed), Manual of Histologic Staining Methods of the AFIP, 3rd edition, McGraw-Hill, NY, c 1968

Lillies, R.D. Fullmer, H.M., Histopathologic Technic and Pratical Histochemistry 4th ed., McGraw-Hill, N.Y. 1976, pp 670-671.

Clark, G., (ed). Staining Procedures, 3rd edition, Williams & Wilkins, Baltimore, p. 52, c.1973.

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Lacquers's Method for Alcoholic Hyalin