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Electron Microscopy Sciences

Technical Data Sheets

Carstairs' Method for Fibrin & Platelets

EMS Catalog #: 26381-Series    

Fixation:

Formalin-Saline for 48 hours or more.

Sections:

Cut paraffin at 5 µm sections.

Staining Procedures:

  1. Hydrate slides to distilled water.
  2. Mordant in Ferric Ammonium Sulfate, 5% (#26381-01) for 5 minutes. Rinse in running tap water.
  3. Stain with Mayer Hematoxylin (#26381-02) for 5 minutes. Rinse in running tap water.
  4. Stain in the Picric Acid-Orange G Solution (#26381-03) for 30 minutes to 1 hour. Rinse once in distilled water.
  5. Stain in Ponceau Fuchsin Solution (#26381-04) for 1 to 5 minutes. Rinse in distilled water.
  6. Differentiate with Phosphotungstic Acid, 1% (#26381-05) until the muscle is red and background pale pink. Rinse in distilled water.
  7. Stain with Aniline Blue Solution (#26381-06) for 30 minutes to 2 hours. Rinse in several changes of distilled water.
  8. Dehydrate, clear in Xylene and coverslip, using a synthetic mounting medium.

Stain Results

Fixation Time: 48 Hours or more Less than 48 hours
Fibrin Bright Red Orange to Orange Red
Platelets Gray Blue or Navy Light Gray
Collagen Bright Blue Bright Blue
Muscle Red Red
Red Blood Cells Clear Yellow Red, Green or Yellow

Product Information

Carstairs' Method for Fibrin & Platelets