Modified
Movats Stain
EMS Catalog #26385
Fixation
10% Buffered Neutral Formalin or Bouin's Solution
Note: If formalin fixative is used, decerated tissue sections must be mordanted in Bouins Fluid for 1 hour in a 50 °C oven. Wash well in running water to remove picric acid deposits.
Sections
4-6 microns
Procedure
- Decerate section and hydrate to distilled water.
- Stain in Alcian Blue, 1% for 20 minutes.
- Dip five times in distilled water.
- Place in Alkaline Alcohol in a 56°C oven for 10 minutes.
- Wash in running tap water for 2 minutes.
- Stain in Orcein-Verhoeff working solution for 2 hours.
To prepare Orcein-Verhoeff working solution:
Immediately before use, in the following order, combine the solutions below:
Orcein 0.2% 25.0 ml Alcoholic Hematoxylin 5% 8.0 ml Ferric Chloride 10% 5.0 ml Lugol's Iodine 5.0 ml
- Wash in running tap water for 3 minutes.
- Stain in Woodstain Scarlet- Acid Fuchsin working solution for 2 1/2 minutes.
- Place in Acetic Acid, 0.5% for 30 seconds.
- Differentiate in Phosphotungstic Acid, 5% for 5-10 minutes, well differentiated sections demonstrate colorless collagen and blue-green mucopolysaccharides.
- Rinse in Acetic Acid, 0.5% for 30 seconds.
- Three changes of 100% ethyl alcohol, 1 minute each,
- Stain in Alcoholic Saffron for 8 minutes.
- Dehydrate in 100% ethyl alcohol, two changes.
- Clear in xylene, three changes.
- Coverslip.
Stain Results
| Nuclei | Black |
| Cytoplasm | Red |
| Elastic Fibers | Purple to Black |
| Collagen and Bone | Yellow |
| Mucopolysaccharides | Blue-Green |
| Muscle | Red |
References
Movat, H.Z .,: Demonstation of all C onnecti ve Tissue Elements in a Single S ection.
Arch Path., 60:289-295, 1955.
Roman N., et al: Orcein-Hematoxylin in I odize d Ferric Chloride as a
Stain for E lastic T issue.
Stain Techn ., 42:199-202, 1967.
Silverman Jefferey., A Modified Movat Pentachrome Stain. Histologic , p. 33. 1972
Product Information
Alcian Blue
1%
Alkaline
Alcohol
Orcein
0.2%
Hematoxylin
Alcoholic 5%
Ferric
Chloride 10%
Lugol’s
Iodine
Woodstain
Scarlet-Acid Fuchsin Working Solution
Acetic
Acid 0.5%
Phosphotungstic
Acid 5%
Alcoholic
Saffron
Modified
Movat's Stain
