Maldonado's Method For Pancreatic Islet Cells
EMS Catalog #26458
Fixation
Bouin's without Acetic Acid
Section
Paraffin, @ 6 Microns
Staining Procedure
- Deparaffinize and hydrate to distilled water.
- Stain for 10 minutes in Phloxine Solution, 1% (26202-01) Rinse in distilled water.
- Place for 1 minute in Phosphotungstic Acid, 3% (26458-02). Rinse in distilled water.
- Stain for 30 seconds in Azure II Solution, 0.05% (26458-03) Rinse in distilled water.
-
Stain in Weigert's working solution for 1 minute. Rinse in distilled water.
To prepare Weigert's Working solution:
Mix just before use:
1 part Weigert's A (26458-04)
1 part Weigert's B (26458-05) DO NOT REUSE!!!!! - Dehydrate in 95% alcohol, absolute alcohol and clear in xylene, two changes each.
- Mount with Permount (17985)
Results
Human Tissue |
|
| Alpha granules: | Purple |
| Beta granules: | Violet Blue |
| D-cell granules: | Light blue with evident granules |
| Exocrine cells: | Grayish blue with red granules |
References
Maldonado, R. and San Jose, H. Stain Techn., 42:11-13, 1967
Luna , L.G., (ed.), Manual of Histologic Staining Methods of the AFIP, 3rd edition, McGraw, N.Y.
p. 105, c. 1968
Product Information
Phloxine Solution, 1%
Phosphotungstic Acid, 3%
Azure II Solution, 0.05%
Weigert's A
Weigert's B
Maldonado's Method For Pancreatic Islet Cells
