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Electron Microscopy Sciences

Technical Data Sheets

Nile Blue A

EMS Catalog #26511

Fixation

Formalin fixatives

Application

Show differentiation of melanins and lipofuscins

Staining

  1. Deparaffinize and hydrate to distilled water.
  2. Stain 20 minutes in Nile Blue A Staining solution.
  3. Rinse quickly with sulfuric acid, 1%.
  4. Dehydrate immediately in four changes of acetone, 15 seconds each. Rinse in Xylene-acetone. Clear in two changes of Xylene.
  5. Mount.

Stain Results

Lipofuscins Unstained - natural yellow
Melanins Green

 

References

Staining Procedures, G. Clark: Williams and Wilkins Co., Baltimore, 3 rd ed., c. 1973, p. 122.

Lillie, R.D.: Stain Techn ., 31:151.

Product Information

Nile Blue A Staining Solution
Sulfuric Acid 1% Aqueous