Nile Blue A
EMS Catalog #26511
Fixation
Formalin fixatives
Application
Show differentiation of melanins and lipofuscins
Staining
- Deparaffinize and hydrate to distilled water.
- Stain 20 minutes in Nile Blue A Staining solution.
- Rinse quickly with sulfuric acid, 1%.
- Dehydrate immediately in four changes of acetone, 15 seconds each. Rinse in Xylene-acetone. Clear in two changes of Xylene.
- Mount.
Stain Results
| Lipofuscins | Unstained - natural yellow |
| Melanins | Green |
References
Staining Procedures, G. Clark: Williams and Wilkins Co., Baltimore, 3 rd ed., c. 1973, p. 122.
Lillie, R.D.: Stain Techn ., 31:151.
