Darrow Red
EMS Catalog #26674
Fixation
Will follow most fixatives; Formalin is usually used.
Sections
Paraffin, Frozen or Celloidin
Staining
- Stain well washed Weil stained sections or Kluver-Barrera stained sections (with either of these the sections should be no thicker than 20 µ) or unstained sections for 20 minutes in Darrow Red Staining Solution.
- Pass rapidly through ascending alcohols.
- Two changes of n-butyl alcohol for a total of 4 minutes.
- Terpineol: Xylene (1:4) overnight.
- Clear and mount.
Staining Results
| Nissl Bodies and Nuclear Chromatin | Red |
References
Clark, G.: Staining Procedures , Williams and Wilkins Co., Baltimore , 3 rd Ed., c. 1973, p. 89.
M.M., Clark, G., Darrow, M.A. and Emmel, V.M., Staining Techn ., 35:19-21, 1960.
Powers, M.M. and Clark, G., Stain Techn ., 38:289, 1963.
Ordering
Darrow Red Staining Solution, N-Butyl Alcohol and Terpineol-Xylene 1:4 for Darrow Red are available by calling EMS.
