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Electron Microscopy Sciences

Technical Data Sheets

Relative Acidophilia Stain

EMS Catalog #26901

Fixation

Formalin Solutions

Sections

Paraffin

Staining (Use 5 Slides)

  1. Deparaffinize and hydrate to distilled water.
  2. Stain for all 5 slides in Phioxine B, 0.5% Solution for 30 minutes.
  3. Remove one slide, rinse in water and 95% alcohol and hold in n-Butyl Alcohol. Rinse the remaining 4 slides quickly in water.
  4. Stain the remaining 4 slides in Tartrazine Solution and remove at 2,5, 10 and 20 minutes. Each time, rinse in 95% alcohol and hold in n-Butyl Alcohol following removal.
  5. When all 5 slides are in n-Butyl Alcohol, change to fresh n-Butyl Alcohol for 4 minutes, then clear and mount.

Staining Results

The least acidophilic elements stained in the phloxine control slide lose the red color after 2 minutes in the tartrazine solution. The most acidophilic retain the phloxine after 20 minutes in the tartrazine.

Reference

Lendrum, A.C., J. Path. Bact ., 59:399-404, 1947.

Clark ,G., (ed.), Staining Procedures , 3 rd edition, Williams & Wilkins, Baltimore , p. 163, c 1973.

Product Information

Phloxine B Staining Solution
Tartrazine Saturated Sol. in Cellosolve
n-Butyl Alcohol
Relative Acidophilia Stain