Neat Stain Hematology Stain Kit
EMS #27104-01
This kit is designed for the differentiation of morphological cell types in peripheral blood smears. The staining characteristics are similar to the traditional Wright’s and Wright-Giemsa stains.
The simple three-step procedure is useful for laboratories performing manual staining of microorganisms and spermatozoa. The kit is also well suited for stat procedures.
Each kit contains the following components:
- 4 oz. bottle of QUICK III Fixative Solution
- 4 oz. bottle of QUICK III Solution I
- 4 oz. bottle of QUICK III Solution II
The Neat Stain Hematology Stain Kit contains exceptional stain quality with each reagent in a convenient, wide-mouth reagent bottle with a screw cap lid.
Storage Conditions
Store at controlled room temperature (15-30°C). Keep cuvettes tightly sealed when not in use. Do not use after specified expiration date.
Indications of Deterioration
Mold growth or excessive precipitation in reagents are indications of contamination and or degradation.
Specimen Collection & Preparation
Smears prepared from fresh uncoagulated venous or arterial blood should be air-dried. If the smears are not prepared immediately, 2mgs of EDTA/ml of blood sample should be added to prevent coagulation.
Technique
- Dip the prepared slide into the QUICK III Fixative Solution for 1 second and then remove, repeat for a total of 5 dips total. Ensure that the sample area on the slide is submerged in solution while dipping. Drain or blot the slide carefully to remove excess reagent. (Slides can be dipped directly into the reagent bottle for convenience.)
- Repeat the procedure in Step 1, substituting the QUICK III Solution I.
- Repeat the procedure in Step 1, substituting the QUICK III Solution II.
- Rinse slides with deionized water and allow to dry.
Note:
Note: Staining intensity may be varied by increasing or decreasing the number of dips in Solution I or Solution II. For a more eosinophilic result (red intensity), increase the number of dips into Solution I; for a more basophilic result (blue intensity), increase the number of dips into Solution II. Ideally, five one second dips into each Solution will produce the desired results. Any variation of the procedure other than described above may adversely affect the results.
Stained Specimen Results
| Specimen | Nucleus | Granules | Cytoplasum |
| Erythrocytes | n/a | n/a | Pink |
| Leukocytes Granular | |||
| Polymorphonuclear | |||
| Neutrophils | Purple | Red-lilac | Light pink |
| Eosinophils | Dark blue | Red/orange | Medium blue |
| Basophils | Dark blue | Dark purple | Light blue |
| Leucocytes Nongranular Monocytes | Violet | n/a | Light blue |
| Lymphocytes | Violet | n/a | Medium blue |
| Platelets | n/a | Purple | Lilac |
| Microorganisms | Blue/violet | n/a | n/a |
| Spermatozoa | Dark blue |
Danger
Poison may be fatal or cause blindness if swallowed. Vapor is harmful and will cause eye irritation. Do not breathe vapor. Avoid contact with eyes, skin or clothing. Keep container closed. Use with adequate ventilation. Keep from heat, sparks and open flame. Wash thoroughly after handling.
CANNOT BE MADE NONPOISONOUS.
First Aid:
If swallowed, call a physician immediately. In case of contact with eyes, immediately flush with water for 15 minutes and notify a physician. Wash exposed areas thoroughly after handling.
Online Ordering
Neat Stain Hematology Stain Kit is available online from the EMS Catalog. For ordering or product information, click here.
