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Electron Microscopy Sciences

Technical Data Sheets

Neutralex™ Starter Kit

EMS Catalog #63330-SK


  • Formalin Neutralizer
  • Formalin Neutralizer Test Reagent


The Formalin Neutralizer is ready to use. Warm the Formalin Neutralizer Test Reagent to room temperature before use.


Solution to be treated Quantity of Solution
to be Treated
Formalin Neutralizer
1% Formaldehyde 1 gallon 2 level scoops*
10% Neutral Buffered Formalin 1 liter 2 level scoops
10% Neutral Buffered Formalin 1 gallon 2 level scoops*
* Use scoop provided in kit
  1. Add Formalin Neutralizer as indicated in the table above and mix until dissolved.
  2. Wait at least 10 minutes.
  3. Test neutralized formalin solution.
    1. Add 100µl of neutralized formalin solution to 100µl of Formalin Neutralizer Test Reagent.
    2. Cap the tube – invert 3 times to mix thoroughly.
    3. Wait 5 minutes for color to develop
    4. Test solutions which are colorless, light or medium violet or pink, have been neutralized

Note: Colorless solutions have a formaldehyde content <0.001%. Solutions that yield a deep pink or burgundy color after treatment have a formaldehyde content >0.05% and should be retreated. Repeat Steps 1 to 3.

Run a positive control using untreated formalin waste solution – see Step 3 above. Solution should turn a deep pink to deep burgundy. If solution does not turn a deep burgundy color, contact Electron Microscopy Sciences for assistance.

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