Grid Stick Glue (For recoating Grid-Stick)
EMS Catalog #71178
All About the Grid-Stick Glue
Grid-Stick Glue is a strong adhesive that will adhere to Synaptek Grid-Sticks within various staining applications. Note that the Grid-Stick Glue, once cured and immersed into inorganic solvents, will maintain its adhesiveness. Electron Microscopy Sciences sells this product in 5 ml containers, enough for about 150-200 coatings. This product has a shelf life of approximately 6 months to one year.
What you will need
- Pyrex petri dish (optional)
- Grid Sticks (Cat. #71175)
- Synaptek staining pipettes (Cat. #71177)
- Grid-Stick Glue (Cat. #71178)
- A small applicator (i.e. toothpick; small brush)
- Oven that heats up to 150°C
Instructions
- Open the Grid-Stick Glue and dip just the tip of the small applicator into the adhesive.
- Lightly apply the glue onto the notched side of the GridStick over the length of the slot as well as between each notch.
- Let the Grid-Stick Glue to dry for approximately 20 minutes.
Note: When drying, make sure that the environment is free of dust, particles, or any other contaminants.
- Place the GridStick in a Pyrex petri dish (if available) with the uncoated side facing down and the top edge of the GridStick resting on the glass rim.
Note: This will avoid the glue from sticking to the bottom of the petri dish.
Note: GridSticks that are coated but uncured can be placed into a Synaptek pipette. BE SURE to indicate through labeling or marking that the pipette is not to be used for staining procedures.
- Place the pipette with Grid-Stick Glue (or the petri dish if available) into the oven set at 150°C for approximately 5 to 10 minutes.
- Carefully remove the pipette and let it cool for at least 10 minutes prior to use
Note: If the GridStick does not adhere or loses adhesion, it is an indication of dirt built-up on its surface. As mentioned above, PLEASE make sure that the adhering area is dust-free and completely clear of contaminants.
Note: If the Grid-Stick Glue seems to be too thick or sticky for your grids, you may choose to dilute the product with Xylene. If, on the other hand, a more thick and sticky coating is desired, this may be attained by removing the cap of the Grid-Stick Glue and leaving it off for 20 minutes so that the Xylene can evaporate, increasing the viscosity of the adhesive.
Note: To remove the Grid-Stick Glue from the GridStick, soak the GridStick in Xylene solution for 24 hours. After soaking, clean the GridStick by soaking it in Xylene or 99% Isopropanol. Be sure to wipe using lens-paper. After the process, you may begin the recoating process.
