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Electron Microscopy Sciences

Technical Data Sheets

Application: Screening FISH-Labeled Planaria (Schmidtea Mediterranea)

EMS Catalog #SFA

FISH (fluorescence in situ hybridization) is routinely used to label features in planarians (Schmidtea mediterranea). The NIGHTSEA Model SFA Stereo Microscope Fluorescence Adapter can be added to just about any existing stereo microscope to create a practical system for screening samples for successful preparation prior to moving to higher resolution imaging techniques.

PIWI gene (marker for stem cells) labeled with FAM (fluorescein amidite). Specimens courtesy of Donald Von Kannon, laboratory of Dr. Wendy Beane, Western Michigan University.

  • PIWI SMED labeled with FAM
  • PIWI SMED labeled with FAM
  • PIWI SMED labeled with FAM
  • PIWI SMED labeled with FAM
  • PIWI SMED labeled with FAM

All specimens below courtesy of Lauren Cote, Reddien Laboratory, Whitehead Institute, MIT

Collagen labeled with FITC (fluorescein isothiocyanate)

  • SMED colagen labeled with FITC
  • SMED colagen labeled with FITC
  • SMED colagen labeled with FITC
  • SMED colagen labeled with FITC
  • SMED colagen labeled with FITC

Now the same samples at higher magnification, imaged with the Keyence VHX-5000 with the NIGHTSEA™ fluorescence adapter.

  • SMED NB.221e labeled with Rhodamine

Opsin and wntP-2 labeled with FITC

  • PIWI SMED labeled with FAM
  • PIWI SMED labeled with FAM
  • PIWI SMED labeled with FAM

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