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Electron Microscopy Sciences

Technical Data Sheets

Fluorescence for Coral Recruitment Research

EMS Catalog #SFA

Fluorescence is a valuable tool for research on coral recruitment and survivorship. Fluorescence makes it much easier to locate tiny corals both on the reef and in the lab.

On the reef:

With conventional searching techniques it is essentially impossible to locate juveniles until they are at least 5-10mm in diameter. By this time they are between 6 months and a year old. This misses an important early part of their life history and makes it difficult to estimate survivorship in natural conditions.

Using dive lights you can use fluorescence to search for coral recruits and juveniles both at night and in the daytime. Detecting small things is all about contrast, and fluorescence brings juvenile corals out in strong contrast. If a coral fluoresces it will generally appear as a bright green spot against a dark background. Researchers can find corals 5mm in diameter from more than 2 meters away, and corals as small as 1mm in diameter in routine sweeps of patches of reef.

  • stereo microscope fluorescence
  • coral recruits

In the lab:

Researchers often deploy settlement tiles on the reef and collect them later to find out what has settled on them. Fluorescence makes it easier to find corals on the settlement tiles. The images below are white-light and fluorescence images of a settlement tile that had been deployed on the reef in Bonaire. It helps to inspect them under a stereo microscope, and it is now easy to add fluorescence with the NIGHTSEA Stereo Microscope Fluorescence Adapter. Fluorescence is also valuable when inspecting the tiles under the microscope.

  • Settlement tile – white light. Photograph © NIGHTSEA/Charles Mazel
  • Settlement tile – fluorescence. Photograph © NIGHTSEA/Charles Mazel
  • Coral polyp on settlement tile – white light.
  • Coral polyp on settlement tile – fluorescence.

Several publications deal specifically with this application of fluorescence:

Baird, A. H., A. Salih, and A. Trevor-Jones, 2006. Fluorescence census techniques for the early detection of coral recruits. Coral Reefs, 25:73-76.

Hart, J. R., 2011. Coral recruitment on a high-latitude reef at Sodwana Bay, South Africa: Research methods and dynamics. MSc thesis, University of Kwa-Zulu, Natal, 81pp.

Korzen, L., A. Israel, and A. Abelson, 2011. Grazing effects of fish versus sea urchins on turf algae and coral recruits: Possible implications for coral reef resilience and restoration. Journal of Marine Biology, Vol. 2011, Article ID 960207 doi:10.1155/2011/960207.

Piniak, G. A., N. D. Fogarty, C. M. Addison, and J. Kenworthy, 2005. Fluorescence census techniques for coral recruits. Coral Reefs, 24:496-500.

Roth, M. S., and N. Knowlton, 2009. Distribution, abundance, and microhabitat characterization of small juvenile corals at Palmyra Atoll. Mar. Ecol. Prog. Ser., 376:133-142.

Salinas-de-León, P., A. Costales-Carrera, S. Zeljkovic, D. J. Smith, and J. J. Bell, 2011. Scleractinian settlement patterns to natural cleared reef substrata and artificial settlement panels on an Indonesian coral reef. Estuarine, Coastal and Shelf Science, 93: 80-85.

Salinas-de-León, P., C. Dryden, D. J. Smith, and J. J. Bell, 2013. Temporal and spatial variability in coral recruitment on two Indonesian coral reefs: consistently lower recruitment to a degraded reef. Marine Biology, 160(1): 97-105.

Schmidt-Roach, S., A. Kunzmann and P. Martinez Abrizu, 2008. In situ observation of coral recruitment using fluorescence census techniques. JEMBE, 367:37-40.

Answers to some common questions:

Can you find ALL recruits with fluorescence?

No. Not all coral recruits fluoresce, and some that do fluoresce do not glow brightly enough to be found easily. Keep in mind that not all adult corals fluoresce either, and even within a species there may be both fluorescent and non-fluorescent morphs.

How far away can you spot a 1mm recruit?

From our own experience, we have certainly seen many, many small bright dots from a meter or more away without looking very hard.

If you do find a small (1-2mm) fluorescing feature, is it definitely a coral?

Not necessarily. There are many small non-corals that fluoresce, including anemones, corallimorphs, zoanthids, hydroids, etc. Even small mobile invertebrates such as some polychaetes fluoresce. It can be a challenge to know what is a coral and what is not.

How do you identify what you have found?

Identifying to species difficult to impossible. In the field you at least want to be able to distinguish coral from non-coral. A good magnifier is recommended.

Do you have to dive at night?

No. Using dive lights you can find coral recruits in the daytime.

Does fluorescence work with settlement tiles?

Yes, using dive lights to inspect settlement tiles visually, or use fluorescence photography to systematically document growth on the tiles. Fluorescence also works well when inspecting settlement tiles under a stereomicroscope. NIGHTSEA offers a simple adapter that adds a fluorescence capabillity to just about any existing stereomicroscope.

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