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Electron Microscopy Sciences

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Fluorophore-Infiltrated Resin Microscopy (FIRM)

(E) is a published TEM image drosophila photoreceptors (http://www.pnas.org/content/105/7/2693.full) at same magnification as (D).

  • Human anterior pituitary, FIRM, rhodamine channel
    Note that the fluorophore illuminates the LR White resin, providing an image of tissue structure primarily in negative relief. Bar,100 µm. All subsequent FIRM images are shown in monochrome.
  • Human anterior pituitary
    FIRM image taken with 150X glycerin objective. Individual secretory granules are easily visualized, ranging from 150-350nm in diameter. Scale bar, 5 µm.
  • Human anterior pituitary
    Formalin-fixed autopsy specimen, comparison of H&E paraffin section (left) with FIRM (right). 20x dry objective used for both. Scale bar, 20 µm.
  • Correlative FIRM/fluorescent lectin labeling Figure 4
    A single LR White thin section was labeled first with Concanavalin AAlexa-488 (B) imaged, then stained for FIRM imaging (A). Densely granulated cells (arrows in A) are strongly labeled with Con-A (arrows in B). Dark structures resembling large lysosomes or residual bodies (arrowheads in A) are also strongly labeled by Con-A, consistent with reports in the literature.
  • Toluidine Blue: Bright Field Figure 5 A
    FIGURE 5: Comparison of toluidine blue staining (A) to FIRM (B) on near adjacent sections of human kidney. Note enhanced contrast, especially of glomerular basement membranes in FIRM image. High power FIRM image (C) of glomerulus. Note resolution of podocyte foot processes (arrows). Scale bars in A, B, 20 µm, in C, 5 µm.
  • Toluidine Blue: FIRM Figure 5 B
    FIGURE 5: Comparison of toluidine blue staining (A) to FIRM (B) on near adjacent sections of human kidney. Note enhanced contrast, especially of glomerular basement membranes in FIRM image. High power FIRM image (C) of glomerulus. Note resolution of podocyte foot processes (arrows). Scale bars in A, B, 20 µm, in C, 5 µm.
  • Toluidine Blue: FIRM Figure 5 C
    FIGURE 5: Comparison of toluidine blue staining (A) to FIRM (B) on near adjacent sections of human kidney. Note enhanced contrast, especially of glomerular basement membranes in FIRM image. High power FIRM image (C) of glomerulus. Note resolution of podocyte foot processes (arrows). Scale bars in A, B, 20 µm, in C, 5 µm.
  • FIRM Arteriole
  • FIRM Peripheral Nerve
  • FIRM Drosophila Eye
  • FIRM Drosophila Eye
  • TEM Drosophila Eye
    a published TEM image of drosophila photoreceptors (http://www.pnas.org/content/105/7/2693.full) at same magnification
 

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