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Electron Microscopy Sciences

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NIGHTSEA Model SFA adapter system for Hirox Fluorescence Images

The NIGHTSEA Model SFA adapter system adds a versatile fluorescence capability to the Hirox digital microscope series. It can be used in a wide variety of applications, as illustrated by the examples below.

Electronic component failure analysis

Examining electronic components that was embedded in epoxy, cross-sectioned, polished, and highlighted with a fluorescent epoxy-like dye. While the manufacturer of the fluorescent dye recommended excitation with ultraviolet light, our Royal Blue light head was used for these images, and was found to be superior to ultraviolet.

  • Fluorescent penetrant highlighting cracks in integrated circuit, 80x, white light (top) and fluorescence under Royal Blue excitation

Circuit board conformal coating

Conformal coating on a circuit board, with damage. Images made with Ultraviolet excitation (left) and white light.

  • Conformal coating on circuit board, 20x, fluorescence under UV excitation and white light

Concrete thin section

The images below were made from a 20µm concrete thin section with the NIGHTSEA Royal Blue excitation. As with the electronic components above, the manufacturer of the fluorescent dye recommended excitation with ultraviolet light. Our Royal Blue light head was used for these images and was found to be superior to ultraviolet.

  • 20x
  • 50x

Thin section courtesy of Department of Mineralogy and Geochemistry, Institute of Geoscience and Geography, University Halle-Wittenberg, Germany.

Epoxy on a motor shaft

Small motor shaft with epoxy where it does not belong. Imaged with white light (left) and ultraviolet excitation, 20x magnification.

  • Epoxy on motor shaft, 20x, white light
  •     Epoxy on motor shaft, 20x, UV excitation

Gel defect in a nylon granule

Nylon 6,6 granules can manifest a process defect called "gel" and if there is too much of this in a production batch it can compromise downstream production. The gel shows up as a brighter fluorescent area within the fluorescing granule. Images made with Royal Blue excitation.

  • Nylon granules with gel defect, 20x, white light and fluorescence under Royal Blue excitation
  • Nylon granules with gel defect, 60x, white light and fluorescence under Royal Blue excitation

Naturally fluorescent mineral

This mineral sample, collected at the Sterling Hill Mine in Ogdensburg, New Jersey, contains willemite (green fluorescence), calcite (red fluorescence) and franklinite (black–no fluorescence).

  • Fluorescent mineral, 20x, white light and fluorescence under Royal Blue excitation
  • Fluorescent mineral, 60x, white light and fluorescence under Royal Blue excitation
  •     Fluorescent mineral, 100x, white light and fluorescence under Royal Blue excitation

Bone fragment

Small bone fragment in sand.

  • Bone in sand, 20x, fluorescence under UV excitation (top) and white light

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