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Technical TipElectron Microscopy Sciences

Parafin and Plastic Techniques for Preparation of Bone Marrow Specimen

Karen Noe, B.S., HTL (ASCP)
Muhlenberg Regional Medical center
Plainfield, NJ 07060

Handling of Bone Marrow

Aspirated Marrow Units:

  1. On the floor, about 5ml of marrow aspirate is placed into lavender top B-D vacutainer containing 0.07 ml 15% EDTA and mix the contents quickly and thoroughly.
  2. After arriving in the laboratory, the contents of the B-D tube are then filtered through either templefiber or Histowrap recovering only units.
  3. The units are wrapped in templefiber or Histowrap and placed in a bottle containing working B-5 Fixative. The units are fixed for 1 hour.
  4. The wrapped units are placed in a plastic processing cassette (do not use metal) and stored in 70% ethyl alcohol until processing.

Needle Biopsy of the Marrow:

  1. On the floor the biopsy material is placed in a bottle working B-5 fixative.
  2. After arriving in the laboratory, the biopsy is fixed in a minimum of 30 minutes; 1 hour preferable.
  3. After fixation, rinse the biopsy in tap water.
  4. The specimen is placed in RDO decalcifying solution for 15-30 minutes.
  5. When the decalcification is completed, the specimen is wrapped in lens paper, placed in a plastic bag processing cassette (no metal) and washed in running tap water for 30 minutes.
  6. Process as usual
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